SRX3426759 GSM2867552 SRP125768 PRJNA420091 SRS2718273 SAMN08105415 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867552 GSM2867552 GEO Accession GSM2867552 SRX3426760 GSM2867553 SRP125768 PRJNA420091 SRS2718271 SAMN08105414 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867553 GSM2867553 GEO Accession GSM2867553 SRX3426761 GSM2867554 SRP125768 PRJNA420091 SRS2718272 SAMN08105413 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867554 GSM2867554 GEO Accession GSM2867554 SRX3426762 GSM2867555 SRP125768 PRJNA420091 SRS2718274 SAMN08105412 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867555 GSM2867555 GEO Accession GSM2867555 SRX3426763 GSM2867556 SRP125768 PRJNA420091 SRS2718275 SAMN08105411 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867556 GSM2867556 GEO Accession GSM2867556 SRX3426764 GSM2867557 SRP125768 PRJNA420091 SRS2718276 SAMN08105410 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867557 GSM2867557 GEO Accession GSM2867557 SRX3426765 GSM2867558 SRP125768 PRJNA420091 SRS2718277 SAMN08105409 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867558 GSM2867558 GEO Accession GSM2867558 SRX3426766 GSM2867559 SRP125768 PRJNA420091 SRS2718278 SAMN08105408 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867559 GSM2867559 GEO Accession GSM2867559 SRX3426767 GSM2867560 SRP125768 PRJNA420091 SRS2718279 SAMN08105407 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867560 GSM2867560 GEO Accession GSM2867560 SRX3426768 GSM2867561 SRP125768 PRJNA420091 SRS2718280 SAMN08105406 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867561 GSM2867561 GEO Accession GSM2867561 SRX3426769 GSM2867562 SRP125768 PRJNA420091 SRS2718281 SAMN08105405 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867562 GSM2867562 GEO Accession GSM2867562 SRX3426770 GSM2867563 SRP125768 PRJNA420091 SRS2718282 SAMN08105404 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867563 GSM2867563 GEO Accession GSM2867563 SRX3426771 GSM2867564 SRP125768 PRJNA420091 SRS2718284 SAMN08105403 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867564 GSM2867564 GEO Accession GSM2867564 SRX3426773 GSM2867565 SRP125768 PRJNA420091 SRS2718285 SAMN08105402 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867565 GSM2867565 GEO Accession GSM2867565 SRX3426774 GSM2867566 SRP125768 PRJNA420091 SRS2718286 SAMN08105401 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867566 GSM2867566 GEO Accession GSM2867566 SRX3426775 GSM2867567 SRP125768 PRJNA420091 SRS2718287 SAMN08105400 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867567 GSM2867567 GEO Accession GSM2867567 SRX3426776 GSM2867568 SRP125768 PRJNA420091 SRS2718288 SAMN08105399 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867568 GSM2867568 GEO Accession GSM2867568 SRX3426777 GSM2867569 SRP125768 PRJNA420091 SRS2718289 SAMN08105398 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867569 GSM2867569 GEO Accession GSM2867569 SRX3426778 GSM2867570 SRP125768 PRJNA420091 SRS2718290 SAMN08105397 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867570 GSM2867570 GEO Accession GSM2867570 SRX3426779 GSM2867571 SRP125768 PRJNA420091 SRS2718291 SAMN08105396 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867571 GSM2867571 GEO Accession GSM2867571 SRX3426780 GSM2867572 SRP125768 PRJNA420091 SRS2718292 SAMN08105395 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867572 GSM2867572 GEO Accession GSM2867572 SRX3426781 GSM2867573 SRP125768 PRJNA420091 SRS2718293 SAMN08105394 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867573 GSM2867573 GEO Accession GSM2867573 SRX3426782 GSM2867574 SRP125768 PRJNA420091 SRS2718294 SAMN08105393 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867574 GSM2867574 GEO Accession GSM2867574 SRX3426783 GSM2867575 SRP125768 PRJNA420091 SRS2718295 SAMN08105392 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867575 GSM2867575 GEO Accession GSM2867575 SRX3426784 GSM2867576 SRP125768 PRJNA420091 SRS2718296 SAMN08105391 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867576 GSM2867576 GEO Accession GSM2867576 SRX3426785 GSM2867577 SRP125768 PRJNA420091 SRS2718297 SAMN08105390 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 302867577 GSM2867577 GEO Accession GSM2867577 SRX3426786 GSM2867578 SRP125768 PRJNA420091 SRS2718298 SAMN08105389 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867578 GSM2867578 GEO Accession GSM2867578 SRX3426787 GSM2867579 SRP125768 PRJNA420091 SRS2718299 SAMN08105384 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry NextSeq 500 gds 302867579 GSM2867579 GEO Accession GSM2867579 SRX3426788 GSM2867580 SRP125768 PRJNA420091 SRS2718300 SAMN08105383 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867580 GSM2867580 GEO Accession GSM2867580 SRX3426789 GSM2867581 SRP125768 PRJNA420091 SRS2718301 SAMN08105385 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867581 GSM2867581 GEO Accession GSM2867581 SRX3426790 GSM2867582 SRP125768 PRJNA420091 SRS2718302 SAMN08105387 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867582 GSM2867582 GEO Accession GSM2867582 SRX3426791 GSM2867583 SRP125768 PRJNA420091 SRS2718303 SAMN08105386 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867583 GSM2867583 GEO Accession GSM2867583 SRX3426792 GSM2867584 SRP125768 PRJNA420091 SRS2718304 SAMN08105388 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867584 GSM2867584 GEO Accession GSM2867584 SRX3426793 GSM2867585 SRP125768 PRJNA420091 SRS2718305 SAMN08105382 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867585 GSM2867585 GEO Accession GSM2867585 SRX3426794 GSM2867586 SRP125768 PRJNA420091 SRS2718306 SAMN08105420 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq Illumina HiSeq 4000 gds 302867586 GSM2867586 GEO Accession GSM2867586 SRX3426795 GSM2867587 SRP125768 PRJNA420091 SRS2718307 SAMN08105419 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq NextSeq 500 gds 302867587 GSM2867587 GEO Accession GSM2867587 SRX3426796 GSM2867588 SRP125768 PRJNA420091 SRS2718308 SAMN08105418 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq NextSeq 500 gds 302867588 GSM2867588 GEO Accession GSM2867588 SRX3426797 GSM2867589 SRP125768 PRJNA420091 SRS2718309 SAMN08105417 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq NextSeq 500 gds 302867589 GSM2867589 GEO Accession GSM2867589 SRX3426798 GSM2867590 SRP125768 PRJNA420091 SRS2718310 SAMN08105416 ATAC-seq GENOMIC other Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. Two brains were used for each ATAC-seq reaction, one male and one female. ATAC-seq was performed as previously described in Davie and Jacobs et al 2015 (PLOS Genetics) ATAC-seq NextSeq 500 gds 302867590 GSM2867590 GEO Accession GSM2867590 SRX4084549 GSM3142552 SRP125768 PRJNA420091 SRS3301608 SAMN09210681 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142552 GSM3142552 GEO Accession GSM3142552 SRX4084550 GSM3142553 SRP125768 PRJNA420091 SRS3301609 SAMN09210725 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142553 GSM3142553 GEO Accession GSM3142553 SRX4084551 GSM3142534 SRP125768 PRJNA420091 SRS3301610 SAMN09210696 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 15 hours in a mix of Collagenase (75µl @ 100mg/ml), Dispase (50µl @ 3mg/ml) and Trypsin 0.05% EDTA (125µl), the sample was pipette mixed every 5 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 303142534 GSM3142534 GEO Accession GSM3142534 SRX4084552 GSM3142535 SRP125768 PRJNA420091 SRS3301618 SAMN09210695 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 15 hours in a mix of Collagenase (75µl @ 100mg/ml), Dispase (50µl @ 3mg/ml) and Trypsin 0.05% EDTA (125µl), the sample was pipette mixed every 5 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 303142535 GSM3142535 GEO Accession GSM3142535 SRX4084553 GSM3142536 SRP125768 PRJNA420091 SRS3301611 SAMN09210694 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 15 hours in a mix of Collagenase (75µl @ 100mg/ml), Dispase (50µl @ 3mg/ml) and Trypsin 0.05% EDTA (125µl), the sample was pipette mixed every 5 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry Illumina HiSeq 4000 gds 303142536 GSM3142536 GEO Accession GSM3142536 SRX4084554 GSM3142537 SRP125768 PRJNA420091 SRS3301612 SAMN09210693 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing barcoded CEL-seq2 primers. CEL-seq2 NextSeq 500 gds 303142537 GSM3142537 GEO Accession GSM3142537 SRX4084555 GSM3142538 SRP125768 PRJNA420091 SRS3301613 SAMN09210692 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142538 GSM3142538 GEO Accession GSM3142538 SRX4084556 GSM3142539 SRP125768 PRJNA420091 SRS3301635 SAMN09210691 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142539 GSM3142539 GEO Accession GSM3142539 SRX4084557 GSM3142540 SRP125768 PRJNA420091 SRS3301614 SAMN09210690 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142540 GSM3142540 GEO Accession GSM3142540 SRX4084558 GSM3142541 SRP125768 PRJNA420091 SRS3301615 SAMN09210689 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142541 GSM3142541 GEO Accession GSM3142541 SRX4084559 GSM3142542 SRP125768 PRJNA420091 SRS3301616 SAMN09210688 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142542 GSM3142542 GEO Accession GSM3142542 SRX4084560 GSM3142543 SRP125768 PRJNA420091 SRS3301617 SAMN09210687 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142543 GSM3142543 GEO Accession GSM3142543 SRX4084561 GSM3142544 SRP125768 PRJNA420091 SRS3301623 SAMN09210686 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142544 GSM3142544 GEO Accession GSM3142544 SRX4084562 GSM3142545 SRP125768 PRJNA420091 SRS3301619 SAMN09210680 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142545 GSM3142545 GEO Accession GSM3142545 SRX4084563 GSM3142546 SRP125768 PRJNA420091 SRS3301620 SAMN09210679 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142546 GSM3142546 GEO Accession GSM3142546 SRX4084564 GSM3142547 SRP125768 PRJNA420091 SRS3301621 SAMN09210678 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142547 GSM3142547 GEO Accession GSM3142547 SRX4084565 GSM3142548 SRP125768 PRJNA420091 SRS3301622 SAMN09210685 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142548 GSM3142548 GEO Accession GSM3142548 SRX4084566 GSM3142549 SRP125768 PRJNA420091 SRS3301636 SAMN09210684 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142549 GSM3142549 GEO Accession GSM3142549 SRX4084567 GSM3142550 SRP125768 PRJNA420091 SRS3301624 SAMN09210683 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142550 GSM3142550 GEO Accession GSM3142550 SRX4084568 GSM3142551 SRP125768 PRJNA420091 SRS3301625 SAMN09210682 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142551 GSM3142551 GEO Accession GSM3142551 SRX4084569 GSM3142554 SRP125768 PRJNA420091 SRS3301626 SAMN09210724 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142554 GSM3142554 GEO Accession GSM3142554 SRX4084570 GSM3142555 SRP125768 PRJNA420091 SRS3301628 SAMN09210723 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142555 GSM3142555 GEO Accession GSM3142555 SRX4084571 GSM3142556 SRP125768 PRJNA420091 SRS3301627 SAMN09210677 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142556 GSM3142556 GEO Accession GSM3142556 SRX4084572 GSM3142557 SRP125768 PRJNA420091 SRS3301629 SAMN09210676 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142557 GSM3142557 GEO Accession GSM3142557 SRX4084573 GSM3142558 SRP125768 PRJNA420091 SRS3301630 SAMN09210675 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142558 GSM3142558 GEO Accession GSM3142558 SRX4084574 GSM3142559 SRP125768 PRJNA420091 SRS3301647 SAMN09210674 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142559 GSM3142559 GEO Accession GSM3142559 SRX4084575 GSM3142560 SRP125768 PRJNA420091 SRS3301673 SAMN09210673 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142560 GSM3142560 GEO Accession GSM3142560 SRX4084576 GSM3142561 SRP125768 PRJNA420091 SRS3301631 SAMN09210722 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142561 GSM3142561 GEO Accession GSM3142561 SRX4084577 GSM3142562 SRP125768 PRJNA420091 SRS3301632 SAMN09210721 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142562 GSM3142562 GEO Accession GSM3142562 SRX4084578 GSM3142563 SRP125768 PRJNA420091 SRS3301633 SAMN09210720 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142563 GSM3142563 GEO Accession GSM3142563 SRX4084579 GSM3142564 SRP125768 PRJNA420091 SRS3301634 SAMN09210672 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142564 GSM3142564 GEO Accession GSM3142564 SRX4084580 GSM3142565 SRP125768 PRJNA420091 SRS3301653 SAMN09210671 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142565 GSM3142565 GEO Accession GSM3142565 SRX4084581 GSM3142566 SRP125768 PRJNA420091 SRS3301658 SAMN09210670 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142566 GSM3142566 GEO Accession GSM3142566 SRX4084582 GSM3142567 SRP125768 PRJNA420091 SRS3301637 SAMN09210669 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142567 GSM3142567 GEO Accession GSM3142567 SRX4084583 GSM3142568 SRP125768 PRJNA420091 SRS3301638 SAMN09210668 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142568 GSM3142568 GEO Accession GSM3142568 SRX4084584 GSM3142569 SRP125768 PRJNA420091 SRS3301640 SAMN09210667 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142569 GSM3142569 GEO Accession GSM3142569 SRX4084585 GSM3142570 SRP125768 PRJNA420091 SRS3301639 SAMN09210666 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142570 GSM3142570 GEO Accession GSM3142570 SRX4084586 GSM3142571 SRP125768 PRJNA420091 SRS3301641 SAMN09210665 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142571 GSM3142571 GEO Accession GSM3142571 SRX4084587 GSM3142572 SRP125768 PRJNA420091 SRS3301642 SAMN09210664 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142572 GSM3142572 GEO Accession GSM3142572 SRX4084588 GSM3142573 SRP125768 PRJNA420091 SRS3301643 SAMN09210663 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142573 GSM3142573 GEO Accession GSM3142573 SRX4084589 GSM3142574 SRP125768 PRJNA420091 SRS3301644 SAMN09210662 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142574 GSM3142574 GEO Accession GSM3142574 SRX4084590 GSM3142575 SRP125768 PRJNA420091 SRS3301645 SAMN09210661 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142575 GSM3142575 GEO Accession GSM3142575 SRX4084591 GSM3142576 SRP125768 PRJNA420091 SRS3301646 SAMN09210660 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142576 GSM3142576 GEO Accession GSM3142576 SRX4084592 GSM3142577 SRP125768 PRJNA420091 SRS3301648 SAMN09210659 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142577 GSM3142577 GEO Accession GSM3142577 SRX4084593 GSM3142578 SRP125768 PRJNA420091 SRS3301649 SAMN09210658 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142578 GSM3142578 GEO Accession GSM3142578 SRX4084594 GSM3142579 SRP125768 PRJNA420091 SRS3301650 SAMN09210657 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142579 GSM3142579 GEO Accession GSM3142579 SRX4084595 GSM3142580 SRP125768 PRJNA420091 SRS3301651 SAMN09210656 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142580 GSM3142580 GEO Accession GSM3142580 SRX4084596 GSM3142581 SRP125768 PRJNA420091 SRS3301652 SAMN09210655 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142581 GSM3142581 GEO Accession GSM3142581 SRX4084597 GSM3142582 SRP125768 PRJNA420091 SRS3301654 SAMN09210654 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq NextSeq 500 gds 303142582 GSM3142582 GEO Accession GSM3142582 SRX4084598 GSM3142583 SRP125768 PRJNA420091 SRS3301655 SAMN09210653 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142583 GSM3142583 GEO Accession GSM3142583 SRX4084599 GSM3142584 SRP125768 PRJNA420091 SRS3301657 SAMN09210652 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142584 GSM3142584 GEO Accession GSM3142584 SRX4084600 GSM3142585 SRP125768 PRJNA420091 SRS3301656 SAMN09210651 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142585 GSM3142585 GEO Accession GSM3142585 SRX4084601 GSM3142586 SRP125768 PRJNA420091 SRS3301660 SAMN09210650 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142586 GSM3142586 GEO Accession GSM3142586 SRX4084602 GSM3142587 SRP125768 PRJNA420091 SRS3301659 SAMN09210649 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142587 GSM3142587 GEO Accession GSM3142587 SRX4084603 GSM3142588 SRP125768 PRJNA420091 SRS3301661 SAMN09210648 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142588 GSM3142588 GEO Accession GSM3142588 SRX4084604 GSM3142589 SRP125768 PRJNA420091 SRS3301662 SAMN09210647 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142589 GSM3142589 GEO Accession GSM3142589 SRX4084605 GSM3142590 SRP125768 PRJNA420091 SRS3301663 SAMN09210646 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142590 GSM3142590 GEO Accession GSM3142590 SRX4084606 GSM3142591 SRP125768 PRJNA420091 SRS3301664 SAMN09210645 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142591 GSM3142591 GEO Accession GSM3142591 SRX4084607 GSM3142592 SRP125768 PRJNA420091 SRS3301666 SAMN09210698 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142592 GSM3142592 GEO Accession GSM3142592 SRX4084608 GSM3142593 SRP125768 PRJNA420091 SRS3301665 SAMN09210697 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142593 GSM3142593 GEO Accession GSM3142593 SRX4084609 GSM3142594 SRP125768 PRJNA420091 SRS3301668 SAMN09210719 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142594 GSM3142594 GEO Accession GSM3142594 SRX4084610 GSM3142595 SRP125768 PRJNA420091 SRS3301667 SAMN09210718 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142595 GSM3142595 GEO Accession GSM3142595 SRX4084611 GSM3142596 SRP125768 PRJNA420091 SRS3301669 SAMN09210717 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142596 GSM3142596 GEO Accession GSM3142596 SRX4084612 GSM3142597 SRP125768 PRJNA420091 SRS3301670 SAMN09210716 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142597 GSM3142597 GEO Accession GSM3142597 SRX4084613 GSM3142598 SRP125768 PRJNA420091 SRS3301671 SAMN09210715 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142598 GSM3142598 GEO Accession GSM3142598 SRX4084614 GSM3142599 SRP125768 PRJNA420091 SRS3301672 SAMN09210714 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142599 GSM3142599 GEO Accession GSM3142599 SRX4084615 GSM3142600 SRP125768 PRJNA420091 SRS3301675 SAMN09210713 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142600 GSM3142600 GEO Accession GSM3142600 SRX4084616 GSM3142601 SRP125768 PRJNA420091 SRS3301674 SAMN09210712 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142601 GSM3142601 GEO Accession GSM3142601 SRX4084617 GSM3142602 SRP125768 PRJNA420091 SRS3301676 SAMN09210711 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142602 GSM3142602 GEO Accession GSM3142602 SRX4084618 GSM3142603 SRP125768 PRJNA420091 SRS3301677 SAMN09210710 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142603 GSM3142603 GEO Accession GSM3142603 SRX4084619 GSM3142604 SRP125768 PRJNA420091 SRS3301678 SAMN09210709 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142604 GSM3142604 GEO Accession GSM3142604 SRX4084620 GSM3142605 SRP125768 PRJNA420091 SRS3301679 SAMN09210708 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142605 GSM3142605 GEO Accession GSM3142605 SRX4084621 GSM3142606 SRP125768 PRJNA420091 SRS3301681 SAMN09210707 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142606 GSM3142606 GEO Accession GSM3142606 SRX4084622 GSM3142607 SRP125768 PRJNA420091 SRS3301682 SAMN09210706 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142607 GSM3142607 GEO Accession GSM3142607 SRX4084623 GSM3142608 SRP125768 PRJNA420091 SRS3301680 SAMN09210705 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142608 GSM3142608 GEO Accession GSM3142608 SRX4084624 GSM3142609 SRP125768 PRJNA420091 SRS3301683 SAMN09210704 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142609 GSM3142609 GEO Accession GSM3142609 SRX4084625 GSM3142610 SRP125768 PRJNA420091 SRS3301684 SAMN09210703 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142610 GSM3142610 GEO Accession GSM3142610 SRX4084626 GSM3142611 SRP125768 PRJNA420091 SRS3301685 SAMN09210702 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142611 GSM3142611 GEO Accession GSM3142611 SRX4084627 GSM3142612 SRP125768 PRJNA420091 SRS3301686 SAMN09210701 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142612 GSM3142612 GEO Accession GSM3142612 SRX4084628 GSM3142613 SRP125768 PRJNA420091 SRS3301687 SAMN09210700 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142613 GSM3142613 GEO Accession GSM3142613 SRX4084629 GSM3142614 SRP125768 PRJNA420091 SRS3301689 SAMN09210699 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142614 GSM3142614 GEO Accession GSM3142614 SRX4084630 GSM3142615 SRP125768 PRJNA420091 SRS3301690 SAMN09210727 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142615 GSM3142615 GEO Accession GSM3142615 SRX4084631 GSM3142616 SRP125768 PRJNA420091 SRS3301688 SAMN09210733 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing RTL buffer. Adapted SMART-Seq NextSeq 500 gds 303142616 GSM3142616 GEO Accession GSM3142616 SRX4084632 GSM3142617 SRP125768 PRJNA420091 SRS3301696 SAMN09210732 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, they were processed using the Nuclei EZ kit and RNA was isolated from collected nuclei using the Ambion RNAqueous-micro kit. Tn5 Mediated RNA-seq Illumina HiSeq 4000 gds 303142617 GSM3142617 GEO Accession GSM3142617 SRX4084633 GSM3142618 SRP125768 PRJNA420091 SRS3301691 SAMN09210731 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, they were processed using the Nuclei EZ kit and RNA was isolated from collected nuclei using the Ambion RNAqueous-micro kit. Tn5 Mediated RNA-seq NextSeq 500 gds 303142618 GSM3142618 GEO Accession GSM3142618 SRX4084634 GSM3142619 SRP125768 PRJNA420091 SRS3301692 SAMN09210730 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, RNA was isolated using the Ambion RNAqueous-micro kit. Illumina Stranded RNA-seq kit NextSeq 500 gds 303142619 GSM3142619 GEO Accession GSM3142619 SRX4084635 GSM3142620 SRP125768 PRJNA420091 SRS3301694 SAMN09210729 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, RNA was isolated using the Ambion RNAqueous-micro kit. Tn5 Mediated RNA-seq NextSeq 500 gds 303142620 GSM3142620 GEO Accession GSM3142620 SRX4084636 GSM3142621 SRP125768 PRJNA420091 SRS3301693 SAMN09210728 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, RNA was isolated using the Ambion RNAqueous-micro kit. Illumina Stranded RNA-seq kit NextSeq 500 gds 303142621 GSM3142621 GEO Accession GSM3142621 SRX4084637 GSM3142622 SRP125768 PRJNA420091 SRS3301695 SAMN09210726 RNA-Seq TRANSCRIPTOMIC cDNA Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, RNA was isolated using the Ambion RNAqueous-micro kit. Tn5 Mediated RNA-seq NextSeq 500 gds 303142622 GSM3142622 GEO Accession GSM3142622