SRX3427485 R16102069 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718937 SAMN08100001 R16102069 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427486 R16102070 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718939 SAMN08100002 R16102070 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427487 R16120900 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718940 SAMN08099999 R16120900 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427488 R16102068 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718938 SAMN08100000 R16102068 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427489 R16102062 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718941 SAMN08099997 R16102062 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427490 R16120896 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718942 SAMN08099998 R16120896 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427491 R16102066 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718943 SAMN08099993 R16102066 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427492 R16102067 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718944 SAMN08099994 R16102067 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427493 R16102061 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718945 SAMN08099991 R16102061 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427494 R16102065 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB843 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718946 SAMN08099992 R16102065 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427495 R16102059 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718947 SAMN08099989 R16102059 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3427496 R16102060 SRP125789 PRJNA419859 Total RNA was isolated from roots of Pearl millet inbred line ICMB863 with Trizol (Sigma-Aldrich, Cat.no:15596026), and genomic DNA was digested with RNase-Free DNase (Qiagen). RNA degradation and potential contamination was checked by gel electrophoresis. RNA integrity number (RIN) and quantitation check was performed by Agilent 2100 bioanalyzer. After the Quality check procedures, mRNA from roots was enriched using oligo(dT) beads. Company name First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers, after which a custom second strand synthesis buffer (Illumina), dNTPs, Rnase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment. The qualified libraries were subjected to sequencing by Illumina Hiseq through NOVOgene Co.,ltd. SRS2718948 SAMN08099990 R16102060 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500