SRX3441545 RA2334-d17-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732358 RA2334-d17-liver-M1 RA2334-d17-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441546 RA2334-PBMC-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732357 RA2334-PBMC-liver-M1 RA2334-PBMC-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441547 RA1866-d129-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732359 RA1866-d129-liver-M1 RA1866-d129-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441548 RA1866-d17-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732360 RA1866-d17-liver-M1 RA1866-d17-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441549 RA2125-PBMC-liver-M2-5020267 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732361 RA2125-PBMC-liver-M2-5020267 RA2125-PBMC-liver-M2-5020267 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441550 RA2125-PBMC-liver-M2-41164165 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732362 RA2125-PBMC-liver-M2-41164165 RA2125-PBMC-liver-M2-41164165 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441551 RA1829-d17-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732363 RA1829-d17-liver-M1 RA1829-d17-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441552 RA1829-PBMC-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732364 RA1829-PBMC-M1 RA1829-PBMC-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441553 RA2125-d18-liver-M2-41164165 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732365 RA2125-d18-liver-M2-41164165 RA2125-d18-liver-M2-41164165 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441554 RA2125-PBMC-liver-M2-69311382 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732366 RA2125-PBMC-liver-M2-69311382 RA2125-PBMC-liver-M2-69311382 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441555 GUIDEseq-A097WL-control SRP126071 PRJNA420894 GUIDEseq SRS2732413 GUIDEseq-A097WL-control GUIDEseq-A097WL-control AMPLICON GENOMIC PCR Illumina MiSeq SRX3441556 GUIDEseq-A109WL SRP126071 PRJNA420894 GUIDEseq SRS2732367 GUIDEseq-A109WL GUIDEseq-A109WL AMPLICON GENOMIC PCR Illumina MiSeq SRX3441557 RA2343-d18-liver-M2-41164165 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732368 RA2343-d18-liver-M2-41164165 RA2343-d18-liver-M2-41164165 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441558 GUIDEseq-A097WL-Meganuclease 2 SRP126071 PRJNA420894 GUIDEseq SRS2732369 GUIDEseq-A097WL-Meganuclease 2 GUIDEseq-A097WL-Meganuclease 2 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441559 GUIDEseq-A122WL SRP126071 PRJNA420894 GUIDEseq SRS2732370 GUIDEseq-A122WL GUIDEseq-A122WL AMPLICON GENOMIC PCR Illumina MiSeq SRX3441560 GUIDEseq-A097WL-Meganuclease 1 SRP126071 PRJNA420894 GUIDEseq SRS2732371 GUIDEseq-A097WL-Meganuclease 1 GUIDEseq-A097WL-Meganuclease 1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441561 GUIDEseq-A075VC SRP126071 PRJNA420894 GUIDEseq SRS2732372 GUIDEseq-A075VC GUIDEseq-A075VC AMPLICON GENOMIC PCR Illumina MiSeq SRX3441562 RA2343-d18-liver-M2-69311382 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732373 RA2343-d18-liver-M2-69311382 RA2343-d18-liver-M2-69311382 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441563 RA2343-d18-liver-M2 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732374 RA2343-d18-liver-M2 RA2343-d18-liver-M2 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441564 RA1857-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732376 RA1857-PBMC-417 RA1857-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441565 RA1857-d129-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732375 RA1857-d129-liver-417 RA1857-d129-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441566 RA1866-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732377 RA1866-PBMC-417 RA1866-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441567 RA1866-d129-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732378 RA1866-d129-liver-417 RA1866-d129-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441568 RA1857-PBMC-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732380 RA1857-PBMC-251 RA1857-PBMC-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441569 RA1866-PBMC-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732379 RA1866-PBMC-251 RA1866-PBMC-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441570 RA1857-d17-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732381 RA1857-d17-liver-251 RA1857-d17-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441571 RA1866-d17-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732382 RA1866-d17-liver-251 RA1866-d17-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441572 RA1857-d129-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732383 RA1857-d129-liver-251 RA1857-d129-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441573 RA1866-d129-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732384 RA1866-d129-liver-251 RA1866-d129-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441574 RA2343-d128-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732385 RA2343-d128-liver-251 RA2343-d128-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441575 AMP-Seq SRP126071 PRJNA420894 On-Target AMP-Seq SRS2732386 AMP-Seq AMP-Seq AMPLICON GENOMIC PCR Illumina MiSeq SRX3441576 RA2343-PBMC-liver-M2 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732387 RA2343-PBMC-liver-M2 RA2343-PBMC-liver-M2 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441577 RA2125-d187-liver-M2 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732388 RA2125-d187-liver-M2 RA2125-d187-liver-M2 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441578 RA1857-d129-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732390 RA1857-d129-liver-M1 RA1857-d129-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441579 RA2125-PBMC-liver-M2 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732389 RA2125-PBMC-liver-M2 RA2125-PBMC-liver-M2 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441580 RA1857-PBMC-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732391 RA1857-PBMC-liver-M1 RA1857-PBMC-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441581 RA2125-d18-liver-M2-5020267 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732392 RA2125-d18-liver-M2-5020267 RA2125-d18-liver-M2-5020267 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441582 RA1857-d17-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732393 RA1857-d17-liver-M1 RA1857-d17-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441583 RA2343-PBMC-M2-5020267 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732394 RA2343-PBMC-M2-5020267 RA2343-PBMC-M2-5020267 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441584 RA2343-PBMC-M2-69311382 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732395 RA2343-PBMC-M2-69311382 RA2343-PBMC-M2-69311382 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441585 RA2343-d18-liver-M2-5020267 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732396 RA2343-d18-liver-M2-5020267 RA2343-d18-liver-M2-5020267 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441586 RA2343-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732397 RA2343-PBMC-417 RA2343-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441587 RA1829-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732398 RA1829-PBMC-417 RA1829-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441588 RA1857-d17-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732400 RA1857-d17-liver-417 RA1857-d17-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441589 RA1866-d17-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732399 RA1866-d17-liver-417 RA1866-d17-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441590 RA2334-d17-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732401 RA2334-d17-liver-417 RA2334-d17-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441591 RA2125-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732402 RA2125-PBMC-417 RA2125-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441592 RA1829-d17-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732403 RA1829-d17-liver-417 RA1829-d17-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441593 RA2334-PBMC-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732404 RA2334-PBMC-417 RA2334-PBMC-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441594 RA1866-PBMC-liver-M1 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732405 RA1866-PBMC-liver-M1 RA1866-PBMC-liver-M1 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441595 RA2125-d18-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732406 RA2125-d18-liver-417 RA2125-d18-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441596 RA2343-d18-liver-417 SRP126071 PRJNA420894 On-Target 417-bp Amplicon SRS2732407 RA2343-d18-liver-417 RA2343-d18-liver-417 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441597 RA2125-d18-liver-M2-69311382 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732408 RA2125-d18-liver-M2-69311382 RA2125-d18-liver-M2-69311382 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441598 RA2125-d128-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732409 RA2125-d128-liver-251 RA2125-d128-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441599 RA2334-d129-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732410 RA2334-d129-liver-251 RA2334-d129-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441600 RA2343-PBMC-M2-41164165 SRP126071 PRJNA420894 In vivo off-target Validation SRS2732411 RA2343-PBMC-M2-41164165 RA2343-PBMC-M2-41164165 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441601 RA2334-PBMC-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732412 RA2334-PBMC-251 RA2334-PBMC-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441602 RA1829-PBMC-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732414 RA1829-PBMC-251 RA1829-PBMC-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441603 RA2334-d17-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732415 RA2334-d17-liver-251 RA2334-d17-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441604 RA1829-d17-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732416 RA1829-d17-liver-251 RA1829-d17-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441605 RA2125-d18-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732417 RA2125-d18-liver-251 RA2125-d18-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441606 RA2343-PBMC-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732418 RA2343-PBMC-251 RA2343-PBMC-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441607 RA1829-d129-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732419 RA1829-d129-liver-251 RA1829-d129-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq SRX3441608 RA2343-d18-liver-251 SRP126071 PRJNA420894 On-Target 251-bp Amplicon SRS2732420 RA2343-d18-liver-251 RA2343-d18-liver-251 AMPLICON GENOMIC PCR Illumina MiSeq