SRX3451499 soil_ITS2_trindade SRP126269 PRJNA421085 DNA was extracted with power soil DNA extraction kits from MoBio, pcr was conducted with primers ITS1F and ITS2 from Smith, D. P., & Peay, K. G. (2014). Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing.PLoS One, 9(2), e90234. We have 7 samples with the following barcodes: #SampleID BarcodeSequence NR1 TTAAGACAGTCG CCGCTGCGTTCTTCATCGATGC NR2 TCTGCACTGAGC CCGCTGCGTTCTTCATCGATGC NR3 CGCAGATTAGTA CCGCTGCGTTCTTCATCGATGC CR1 TGGGTCCCACAT CCGCTGCGTTCTTCATCGATGC CR2 CACTGGTGCATA CCGCTGCGTTCTTCATCGATGC CR3 AACGTAGGCTCT CCGCTGCGTTCTTCATCGATGC CR4 AGTTGTAGTCCG CCGCTGCGTTCTTCATCGATGC SRS2740360 ITS2_fungi_soil soil_ITS2_trindade AMPLICON METAGENOMIC PCR Illumina MiSeq