SRX3452888 GSM2879336 SRP126298 SRS2741641 GSM2879336 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879336 GSM2879336 GEO Accession GSM2879336 SRX3452889 GSM2879337 SRP126298 SRS2741640 GSM2879337 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879337 GSM2879337 GEO Accession GSM2879337 SRX3452890 GSM2879338 SRP126298 SRS2741644 GSM2879338 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879338 GSM2879338 GEO Accession GSM2879338 SRX3452891 GSM2879339 SRP126298 SRS2741642 GSM2879339 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879339 GSM2879339 GEO Accession GSM2879339 SRX3452892 GSM2879340 SRP126298 SRS2741643 GSM2879340 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879340 GSM2879340 GEO Accession GSM2879340 SRX3452893 GSM2879341 SRP126298 SRS2741645 GSM2879341 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879341 GSM2879341 GEO Accession GSM2879341 SRX3452894 GSM2879342 SRP126298 SRS2741646 GSM2879342 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879342 GSM2879342 GEO Accession GSM2879342 SRX3452895 GSM2879343 SRP126298 SRS2741647 GSM2879343 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879343 GSM2879343 GEO Accession GSM2879343 SRX3452896 GSM2879344 SRP126298 SRS2741648 GSM2879344 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879344 GSM2879344 GEO Accession GSM2879344 SRX3452897 GSM2879345 SRP126298 SRS2741649 GSM2879345 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879345 GSM2879345 GEO Accession GSM2879345 SRX3452898 GSM2879346 SRP126298 SRS2741650 GSM2879346 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879346 GSM2879346 GEO Accession GSM2879346 SRX3452899 GSM2879347 SRP126298 SRS2741651 GSM2879347 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879347 GSM2879347 GEO Accession GSM2879347 SRX3452900 GSM2879348 SRP126298 SRS2741652 GSM2879348 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879348 GSM2879348 GEO Accession GSM2879348 SRX3452901 GSM2879349 SRP126298 SRS2741653 GSM2879349 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879349 GSM2879349 GEO Accession GSM2879349 SRX3452902 GSM2879350 SRP126298 SRS2741655 GSM2879350 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879350 GSM2879350 GEO Accession GSM2879350 SRX3452903 GSM2879351 SRP126298 SRS2741654 GSM2879351 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879351 GSM2879351 GEO Accession GSM2879351 SRX3452904 GSM2879352 SRP126298 SRS2741656 GSM2879352 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879352 GSM2879352 GEO Accession GSM2879352 SRX3452905 GSM2879353 SRP126298 SRS2741657 GSM2879353 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879353 GSM2879353 GEO Accession GSM2879353 SRX3452906 GSM2879354 SRP126298 SRS2741658 GSM2879354 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879354 GSM2879354 GEO Accession GSM2879354 SRX3452907 GSM2879355 SRP126298 SRS2741659 GSM2879355 RNA-Seq TRANSCRIPTOMIC cDNA Mice were sacrificed and spinal cords were isolated and transferred to ice-cold PBS (Sigma-Aldrich). Tissue was then cut in small pieces and digested in RPMI containing 2% fetal calf serum (Sigma-Aldrich), 25mM HEPES (Sigma-Aldrich), DNase I (10ng/ml, StemCell Technologies) and Collagenase D (0.8mg/ml, Roche) for 30 min at 37°C. Reaction was stopped by addition 1:100 dilution of a 0.5M EDTA (Sigma-Aldrich) solution. Suspension was filtered through 70 µm cell strainers (Falcon) and resuspended in a 30% solution of Percoll (Sigma-Aldrich). After 30 min of gradient centrifugation at 10.800 r.p.m., the top (myelin) and lower (red cells) layers were removed and the remaining solution was filtered through 70µm cell strainers (Falcon) before further analysis. Cells were stored and shipped in RNAprotect buffer at 2-8 °C. After pelleting, the RNAprotect buffer was replaced by RLT Plus and the samples were homogenized by vortexing for 30 sec. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next, ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease-free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc., Mountain View, CA, USA) was used to generate first strand cDNA from 1 ng total-RNA. Double stranded cDNA was amplified by LD PCR (11 cycles) and purified via magnetic bead clean-up. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step, 1:5 dilutions of index 1 (i7) and index 2 (i5) primers were used. The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Illumina HiSeq 1000 gds 302879355 GSM2879355 GEO Accession GSM2879355