SRX3453027 Basmati SRP126302 PRJNA419635 Whole genome sequencing (WGS) libraries were prepared with Illumina-compatible NEXTflex DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.) at the Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, approx. 1 g of genomic DNA was sheared using Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approx. fragment size distribution from 300 bp to 600 bp. The fragment size distribution was checked on Agilent 2200 TapeStation with D1000 DNA screen tapes and reagents (Agilent Technologies, Palo Alto, CA, USA) and subsequently purified using HighPrep magnetic beads (Magbio Genomics Inc, USA). The purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTFlex DNA sequencing kit protocol. The adapter-ligated DNA was purified with HighPrep beads and then size selected on 2% low melting agarose gel and cleaned using MinElute column (QIAGEN). The resultant fragments were amplified for 10 cycles of PCR using Illumina-compatible primers provided in the NEXTFlex DNA sequencing kit. The final PCR product (sequencing library) was purified with HighPrep beads, followed by library quality control check. The Illumina-compatible sequencing library was initially quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent TapeStation. Finally, the sequencing library was accurately quantified by quantitative PCR using Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). The qPCR quantified library was subjected to sequencing on an Illumina sequencer for 150 bp paired-end chemistry. SRS2741779 SAMN08094039 Basmati WGS GENOMIC PCR Illumina HiSeq 4000