SRX3453237 GSM2879474 SRP126320 SRS2741941 GSM2879474 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879474 GSM2879474 GEO Accession GSM2879474 SRX3453238 GSM2879475 SRP126320 SRS2741942 GSM2879475 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879475 GSM2879475 GEO Accession GSM2879475 SRX3453239 GSM2879476 SRP126320 SRS2741940 GSM2879476 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879476 GSM2879476 GEO Accession GSM2879476 SRX3453240 GSM2879477 SRP126320 SRS2741946 GSM2879477 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879477 GSM2879477 GEO Accession GSM2879477 SRX3453241 GSM2879478 SRP126320 SRS2741943 GSM2879478 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879478 GSM2879478 GEO Accession GSM2879478 SRX3453242 GSM2879479 SRP126320 SRS2741944 GSM2879479 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879479 GSM2879479 GEO Accession GSM2879479 SRX3453243 GSM2879480 SRP126320 SRS2741945 GSM2879480 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879480 GSM2879480 GEO Accession GSM2879480 SRX3453244 GSM2879481 SRP126320 SRS2741949 GSM2879481 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879481 GSM2879481 GEO Accession GSM2879481 SRX3453245 GSM2879482 SRP126320 SRS2741947 GSM2879482 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879482 GSM2879482 GEO Accession GSM2879482 SRX3453246 GSM2879483 SRP126320 SRS2741948 GSM2879483 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879483 GSM2879483 GEO Accession GSM2879483 SRX3453247 GSM2879484 SRP126320 SRS2741950 GSM2879484 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879484 GSM2879484 GEO Accession GSM2879484 SRX3453248 GSM2879485 SRP126320 SRS2741951 GSM2879485 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879485 GSM2879485 GEO Accession GSM2879485 SRX3453249 GSM2879486 SRP126320 SRS2741952 GSM2879486 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879486 GSM2879486 GEO Accession GSM2879486 SRX3453250 GSM2879487 SRP126320 SRS2741953 GSM2879487 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879487 GSM2879487 GEO Accession GSM2879487 SRX3453251 GSM2879488 SRP126320 SRS2741954 GSM2879488 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879488 GSM2879488 GEO Accession GSM2879488 SRX3453252 GSM2879489 SRP126320 SRS2741955 GSM2879489 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879489 GSM2879489 GEO Accession GSM2879489 SRX3453253 GSM2879490 SRP126320 SRS2741956 GSM2879490 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879490 GSM2879490 GEO Accession GSM2879490 SRX3453254 GSM2879491 SRP126320 SRS2741957 GSM2879491 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879491 GSM2879491 GEO Accession GSM2879491 SRX3453255 GSM2879492 SRP126320 SRS2741958 GSM2879492 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879492 GSM2879492 GEO Accession GSM2879492 SRX3453256 GSM2879493 SRP126320 SRS2741961 GSM2879493 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879493 GSM2879493 GEO Accession GSM2879493 SRX3453257 GSM2879494 SRP126320 SRS2741959 GSM2879494 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879494 GSM2879494 GEO Accession GSM2879494 SRX3453258 GSM2879495 SRP126320 SRS2741960 GSM2879495 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879495 GSM2879495 GEO Accession GSM2879495 SRX3453259 GSM2879496 SRP126320 SRS2741963 GSM2879496 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879496 GSM2879496 GEO Accession GSM2879496 SRX3453260 GSM2879497 SRP126320 SRS2741962 GSM2879497 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879497 GSM2879497 GEO Accession GSM2879497 SRX3453261 GSM2879498 SRP126320 SRS2741965 GSM2879498 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879498 GSM2879498 GEO Accession GSM2879498 SRX3453262 GSM2879499 SRP126320 SRS2741964 GSM2879499 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879499 GSM2879499 GEO Accession GSM2879499 SRX3453263 GSM2879500 SRP126320 SRS2741966 GSM2879500 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879500 GSM2879500 GEO Accession GSM2879500 SRX3453264 GSM2879501 SRP126320 SRS2741967 GSM2879501 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879501 GSM2879501 GEO Accession GSM2879501 SRX3453265 GSM2879502 SRP126320 SRS2741968 GSM2879502 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879502 GSM2879502 GEO Accession GSM2879502 SRX3453266 GSM2879503 SRP126320 SRS2741969 GSM2879503 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879503 GSM2879503 GEO Accession GSM2879503 SRX3453267 GSM2879504 SRP126320 SRS2741970 GSM2879504 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879504 GSM2879504 GEO Accession GSM2879504 SRX3453268 GSM2879505 SRP126320 SRS2741971 GSM2879505 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879505 GSM2879505 GEO Accession GSM2879505 SRX3453269 GSM2879506 SRP126320 SRS2741973 GSM2879506 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879506 GSM2879506 GEO Accession GSM2879506 SRX3453270 GSM2879507 SRP126320 SRS2741972 GSM2879507 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879507 GSM2879507 GEO Accession GSM2879507 SRX3453271 GSM2879508 SRP126320 SRS2741974 GSM2879508 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879508 GSM2879508 GEO Accession GSM2879508 SRX3453272 GSM2879509 SRP126320 SRS2741975 GSM2879509 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879509 GSM2879509 GEO Accession GSM2879509 SRX3453273 GSM2879510 SRP126320 SRS2741976 GSM2879510 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879510 GSM2879510 GEO Accession GSM2879510 SRX3453274 GSM2879511 SRP126320 SRS2741977 GSM2879511 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879511 GSM2879511 GEO Accession GSM2879511 SRX3453275 GSM2879512 SRP126320 SRS2741978 GSM2879512 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879512 GSM2879512 GEO Accession GSM2879512 SRX3453276 GSM2879513 SRP126320 SRS2741979 GSM2879513 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879513 GSM2879513 GEO Accession GSM2879513 SRX3453277 GSM2879514 SRP126320 SRS2741980 GSM2879514 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879514 GSM2879514 GEO Accession GSM2879514 SRX3453278 GSM2879515 SRP126320 SRS2741982 GSM2879515 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879515 GSM2879515 GEO Accession GSM2879515 SRX3453279 GSM2879516 SRP126320 SRS2741981 GSM2879516 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879516 GSM2879516 GEO Accession GSM2879516 SRX3453280 GSM2879517 SRP126320 SRS2741983 GSM2879517 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879517 GSM2879517 GEO Accession GSM2879517 SRX3453281 GSM2879518 SRP126320 SRS2741984 GSM2879518 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879518 GSM2879518 GEO Accession GSM2879518 SRX3453282 GSM2879519 SRP126320 SRS2741985 GSM2879519 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879519 GSM2879519 GEO Accession GSM2879519 SRX3453283 GSM2879520 SRP126320 SRS2741986 GSM2879520 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879520 GSM2879520 GEO Accession GSM2879520 SRX3453284 GSM2879521 SRP126320 SRS2741987 GSM2879521 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using the miRNeasy Mini Kit (Qiagen; 217004) according to the manufacturer's protocol, with an additional homogenization and mechanical disruption step using a bead beater (BioSpec, Bartlesville, OK, USA) with Zirconia/Silica beads (BioSpec; 11079101z). RNA quality was assessed using the 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified using a spectrophotometer (DeNovix, Wilmington, DE, USA) and was stored at -80°C until DNase treatment. DNA was removed using the TURBO DNA-free kit (Life Technologies; AM1907) according to the manufacturer's protocol. RNA was quantified and quality assessed, as stated above, and genomic DNA depletion was confirmed using qPCR with 16S rRNA gene primers [19] and iQ SYBR Green Supermix (Bio-Rad; 170-8882) according to the manufacturer's instructions. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumina; MRZGP126) according to the manufacturer's protocol. The quality of rRNA depleted samples was assessed using a 2100 BioAnalyzer prior to processing for sequencing library generation. TruSeq Stranded mRNA Sample Preparation Kit (Illumina; RS-122-2101) was used to prepare the rRNA depleted RNA for sequencing according to the manufacturer's protocol. Libraries were quantified using the Kapa Library Quantification Kit (KapaBiosystems; KK4854) according to the manufacturer's instructions, then normalized and pooled for sequencing according to the Denature and Dilute Libraries Guide for the NextSeq 500 (Illumina; Doc No – 15048776 v02). NextSeq 500 gds 302879521 GSM2879521 GEO Accession GSM2879521