SRP126578 PRJNA422027 We applied pair-end whole-genome sequencing to 9 Leishmania strains corresponding to 4 different species from 8 distinct geographical areas Raw sequence reads In this work we aim to improve our understanding of the complexity and extent of the leishmania evolution in culture. Here we applied whole-genome sequencing of freshly culture adapted field isolates to mine epidemiologically relevant information, including chromosome aneuploidy and gene copy number variant (CNV) profiles. Leishmania