SRX3465319 GSM2886274 SRP126691 SRS2753268 GSM2886274 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886274 GSM2886274 GEO Accession GSM2886274 SRX3465320 GSM2886275 SRP126691 SRS2753272 GSM2886275 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886275 GSM2886275 GEO Accession GSM2886275 SRX3465321 GSM2886276 SRP126691 SRS2753274 GSM2886276 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886276 GSM2886276 GEO Accession GSM2886276 SRX3465322 GSM2886277 SRP126691 SRS2753271 GSM2886277 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886277 GSM2886277 GEO Accession GSM2886277 SRX3465323 GSM2886278 SRP126691 SRS2753269 GSM2886278 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886278 GSM2886278 GEO Accession GSM2886278 SRX3465324 GSM2886279 SRP126691 SRS2753270 GSM2886279 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886279 GSM2886279 GEO Accession GSM2886279 SRX3465325 GSM2886280 SRP126691 SRS2753273 GSM2886280 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886280 GSM2886280 GEO Accession GSM2886280 SRX3465326 GSM2886281 SRP126691 SRS2753275 GSM2886281 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886281 GSM2886281 GEO Accession GSM2886281 SRX3465327 GSM2886282 SRP126691 SRS2753276 GSM2886282 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886282 GSM2886282 GEO Accession GSM2886282 SRX3465328 GSM2886283 SRP126691 SRS2753281 GSM2886283 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886283 GSM2886283 GEO Accession GSM2886283 SRX3465329 GSM2886284 SRP126691 SRS2753278 GSM2886284 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886284 GSM2886284 GEO Accession GSM2886284 SRX3465330 GSM2886285 SRP126691 SRS2753282 GSM2886285 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886285 GSM2886285 GEO Accession GSM2886285 SRX3465331 GSM2886286 SRP126691 SRS2753280 GSM2886286 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886286 GSM2886286 GEO Accession GSM2886286 SRX3465332 GSM2886287 SRP126691 SRS2753277 GSM2886287 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886287 GSM2886287 GEO Accession GSM2886287 SRX3465333 GSM2886288 SRP126691 SRS2753283 GSM2886288 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886288 GSM2886288 GEO Accession GSM2886288 SRX3465334 GSM2886289 SRP126691 SRS2753279 GSM2886289 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886289 GSM2886289 GEO Accession GSM2886289 SRX3465335 GSM2886290 SRP126691 SRS2753284 GSM2886290 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886290 GSM2886290 GEO Accession GSM2886290 SRX3465336 GSM2886291 SRP126691 SRS2753285 GSM2886291 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886291 GSM2886291 GEO Accession GSM2886291 SRX3465337 GSM2886292 SRP126691 SRS2753286 GSM2886292 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886292 GSM2886292 GEO Accession GSM2886292 SRX3465338 GSM2886293 SRP126691 SRS2753292 GSM2886293 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886293 GSM2886293 GEO Accession GSM2886293 SRX3465339 GSM2886294 SRP126691 SRS2753288 GSM2886294 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886294 GSM2886294 GEO Accession GSM2886294 SRX3465340 GSM2886295 SRP126691 SRS2753289 GSM2886295 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886295 GSM2886295 GEO Accession GSM2886295 SRX3465341 GSM2886296 SRP126691 SRS2753287 GSM2886296 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886296 GSM2886296 GEO Accession GSM2886296 SRX3465342 GSM2886297 SRP126691 SRS2753293 GSM2886297 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886297 GSM2886297 GEO Accession GSM2886297 SRX3465343 GSM2886298 SRP126691 SRS2753291 GSM2886298 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886298 GSM2886298 GEO Accession GSM2886298 SRX3465344 GSM2886299 SRP126691 SRS2753290 GSM2886299 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886299 GSM2886299 GEO Accession GSM2886299 SRX3465345 GSM2886300 SRP126691 SRS2753294 GSM2886300 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886300 GSM2886300 GEO Accession GSM2886300 SRX3465346 GSM2886301 SRP126691 SRS2753295 GSM2886301 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886301 GSM2886301 GEO Accession GSM2886301 SRX3465347 GSM2886302 SRP126691 SRS2753296 GSM2886302 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886302 GSM2886302 GEO Accession GSM2886302 SRX3465348 GSM2886303 SRP126691 SRS2753297 GSM2886303 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886303 GSM2886303 GEO Accession GSM2886303 SRX3465349 GSM2886304 SRP126691 SRS2753298 GSM2886304 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886304 GSM2886304 GEO Accession GSM2886304 SRX3465350 GSM2886305 SRP126691 SRS2753303 GSM2886305 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886305 GSM2886305 GEO Accession GSM2886305 SRX3465351 GSM2886306 SRP126691 SRS2753302 GSM2886306 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886306 GSM2886306 GEO Accession GSM2886306 SRX3465352 GSM2886307 SRP126691 SRS2753300 GSM2886307 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886307 GSM2886307 GEO Accession GSM2886307 SRX3465353 GSM2886308 SRP126691 SRS2753299 GSM2886308 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886308 GSM2886308 GEO Accession GSM2886308 SRX3465354 GSM2886309 SRP126691 SRS2753301 GSM2886309 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886309 GSM2886309 GEO Accession GSM2886309 SRX3465355 GSM2886310 SRP126691 SRS2753304 GSM2886310 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886310 GSM2886310 GEO Accession GSM2886310 SRX3465356 GSM2886311 SRP126691 SRS2753305 GSM2886311 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886311 GSM2886311 GEO Accession GSM2886311 SRX3465357 GSM2886312 SRP126691 SRS2753306 GSM2886312 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886312 GSM2886312 GEO Accession GSM2886312 SRX3465358 GSM2886313 SRP126691 SRS2753307 GSM2886313 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886313 GSM2886313 GEO Accession GSM2886313 SRX3465359 GSM2886314 SRP126691 SRS2753308 GSM2886314 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886314 GSM2886314 GEO Accession GSM2886314 SRX3465360 GSM2886315 SRP126691 SRS2753310 GSM2886315 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886315 GSM2886315 GEO Accession GSM2886315 SRX3465361 GSM2886316 SRP126691 SRS2753309 GSM2886316 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886316 GSM2886316 GEO Accession GSM2886316 SRX3465362 GSM2886317 SRP126691 SRS2753311 GSM2886317 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886317 GSM2886317 GEO Accession GSM2886317 SRX3465363 GSM2886318 SRP126691 SRS2753312 GSM2886318 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886318 GSM2886318 GEO Accession GSM2886318 SRX3465364 GSM2886319 SRP126691 SRS2753313 GSM2886319 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886319 GSM2886319 GEO Accession GSM2886319 SRX3465365 GSM2886320 SRP126691 SRS2753314 GSM2886320 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886320 GSM2886320 GEO Accession GSM2886320 SRX3465366 GSM2886321 SRP126691 SRS2753315 GSM2886321 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886321 GSM2886321 GEO Accession GSM2886321 SRX3465367 GSM2886322 SRP126691 SRS2753316 GSM2886322 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886322 GSM2886322 GEO Accession GSM2886322 SRX3465368 GSM2886323 SRP126691 SRS2753320 GSM2886323 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886323 GSM2886323 GEO Accession GSM2886323 SRX3465369 GSM2886324 SRP126691 SRS2753319 GSM2886324 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886324 GSM2886324 GEO Accession GSM2886324 SRX3465370 GSM2886325 SRP126691 SRS2753317 GSM2886325 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886325 GSM2886325 GEO Accession GSM2886325 SRX3465371 GSM2886326 SRP126691 SRS2753318 GSM2886326 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886326 GSM2886326 GEO Accession GSM2886326 SRX3465372 GSM2886327 SRP126691 SRS2753321 GSM2886327 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886327 GSM2886327 GEO Accession GSM2886327 SRX3465373 GSM2886328 SRP126691 SRS2753322 GSM2886328 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886328 GSM2886328 GEO Accession GSM2886328 SRX3465374 GSM2886329 SRP126691 SRS2753324 GSM2886329 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886329 GSM2886329 GEO Accession GSM2886329 SRX3465375 GSM2886330 SRP126691 SRS2753323 GSM2886330 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886330 GSM2886330 GEO Accession GSM2886330 SRX3465376 GSM2886331 SRP126691 SRS2753325 GSM2886331 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886331 GSM2886331 GEO Accession GSM2886331 SRX3465377 GSM2886332 SRP126691 SRS2753327 GSM2886332 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886332 GSM2886332 GEO Accession GSM2886332 SRX3465378 GSM2886333 SRP126691 SRS2753329 GSM2886333 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886333 GSM2886333 GEO Accession GSM2886333 SRX3465379 GSM2886334 SRP126691 SRS2753328 GSM2886334 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886334 GSM2886334 GEO Accession GSM2886334 SRX3465380 GSM2886335 SRP126691 SRS2753330 GSM2886335 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886335 GSM2886335 GEO Accession GSM2886335 SRX3465381 GSM2886336 SRP126691 SRS2753326 GSM2886336 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886336 GSM2886336 GEO Accession GSM2886336 SRX3465382 GSM2886337 SRP126691 SRS2753331 GSM2886337 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886337 GSM2886337 GEO Accession GSM2886337 SRX3465383 GSM2886338 SRP126691 SRS2753332 GSM2886338 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886338 GSM2886338 GEO Accession GSM2886338 SRX3465384 GSM2886339 SRP126691 SRS2753333 GSM2886339 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886339 GSM2886339 GEO Accession GSM2886339 SRX3465385 GSM2886340 SRP126691 SRS2753334 GSM2886340 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886340 GSM2886340 GEO Accession GSM2886340 SRX3465386 GSM2886341 SRP126691 SRS2753335 GSM2886341 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886341 GSM2886341 GEO Accession GSM2886341 SRX3465387 GSM2886342 SRP126691 SRS2753336 GSM2886342 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886342 GSM2886342 GEO Accession GSM2886342 SRX3465388 GSM2886343 SRP126691 SRS2753442 GSM2886343 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886343 GSM2886343 GEO Accession GSM2886343 SRX3465389 GSM2886344 SRP126691 SRS2753337 GSM2886344 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886344 GSM2886344 GEO Accession GSM2886344 SRX3465390 GSM2886345 SRP126691 SRS2753338 GSM2886345 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886345 GSM2886345 GEO Accession GSM2886345 SRX3465391 GSM2886346 SRP126691 SRS2753339 GSM2886346 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886346 GSM2886346 GEO Accession GSM2886346 SRX3465392 GSM2886347 SRP126691 SRS2753341 GSM2886347 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886347 GSM2886347 GEO Accession GSM2886347 SRX3465393 GSM2886348 SRP126691 SRS2753340 GSM2886348 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886348 GSM2886348 GEO Accession GSM2886348 SRX3465394 GSM2886349 SRP126691 SRS2753342 GSM2886349 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886349 GSM2886349 GEO Accession GSM2886349 SRX3465395 GSM2886350 SRP126691 SRS2753343 GSM2886350 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886350 GSM2886350 GEO Accession GSM2886350 SRX3465396 GSM2886351 SRP126691 SRS2753345 GSM2886351 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886351 GSM2886351 GEO Accession GSM2886351 SRX3465397 GSM2886352 SRP126691 SRS2753346 GSM2886352 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886352 GSM2886352 GEO Accession GSM2886352 SRX3465398 GSM2886353 SRP126691 SRS2753347 GSM2886353 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886353 GSM2886353 GEO Accession GSM2886353 SRX3465399 GSM2886354 SRP126691 SRS2753348 GSM2886354 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886354 GSM2886354 GEO Accession GSM2886354 SRX3465400 GSM2886355 SRP126691 SRS2753349 GSM2886355 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886355 GSM2886355 GEO Accession GSM2886355 SRX3465401 GSM2886356 SRP126691 SRS2753351 GSM2886356 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886356 GSM2886356 GEO Accession GSM2886356 SRX3465402 GSM2886357 SRP126691 SRS2753350 GSM2886357 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886357 GSM2886357 GEO Accession GSM2886357 SRX3465403 GSM2886358 SRP126691 SRS2753352 GSM2886358 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886358 GSM2886358 GEO Accession GSM2886358 SRX3465404 GSM2886359 SRP126691 SRS2753353 GSM2886359 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886359 GSM2886359 GEO Accession GSM2886359 SRX3465405 GSM2886360 SRP126691 SRS2753354 GSM2886360 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886360 GSM2886360 GEO Accession GSM2886360 SRX3465406 GSM2886361 SRP126691 SRS2753356 GSM2886361 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886361 GSM2886361 GEO Accession GSM2886361 SRX3465407 GSM2886362 SRP126691 SRS2753357 GSM2886362 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886362 GSM2886362 GEO Accession GSM2886362 SRX3465408 GSM2886363 SRP126691 SRS2753358 GSM2886363 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886363 GSM2886363 GEO Accession GSM2886363 SRX3465409 GSM2886364 SRP126691 SRS2753360 GSM2886364 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886364 GSM2886364 GEO Accession GSM2886364 SRX3465410 GSM2886365 SRP126691 SRS2753361 GSM2886365 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886365 GSM2886365 GEO Accession GSM2886365 SRX3465411 GSM2886366 SRP126691 SRS2753359 GSM2886366 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886366 GSM2886366 GEO Accession GSM2886366 SRX3465412 GSM2886367 SRP126691 SRS2753362 GSM2886367 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886367 GSM2886367 GEO Accession GSM2886367 SRX3465413 GSM2886368 SRP126691 SRS2753363 GSM2886368 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886368 GSM2886368 GEO Accession GSM2886368 SRX3465414 GSM2886369 SRP126691 SRS2753364 GSM2886369 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886369 GSM2886369 GEO Accession GSM2886369 SRX3465415 GSM2886370 SRP126691 SRS2753370 GSM2886370 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886370 GSM2886370 GEO Accession GSM2886370 SRX3465416 GSM2886371 SRP126691 SRS2753367 GSM2886371 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886371 GSM2886371 GEO Accession GSM2886371 SRX3465417 GSM2886372 SRP126691 SRS2753366 GSM2886372 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886372 GSM2886372 GEO Accession GSM2886372 SRX3465418 GSM2886373 SRP126691 SRS2753365 GSM2886373 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886373 GSM2886373 GEO Accession GSM2886373 SRX3465419 GSM2886374 SRP126691 SRS2753371 GSM2886374 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886374 GSM2886374 GEO Accession GSM2886374 SRX3465420 GSM2886375 SRP126691 SRS2753368 GSM2886375 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886375 GSM2886375 GEO Accession GSM2886375 SRX3465421 GSM2886376 SRP126691 SRS2753369 GSM2886376 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886376 GSM2886376 GEO Accession GSM2886376 SRX3465422 GSM2886377 SRP126691 SRS2753511 GSM2886377 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886377 GSM2886377 GEO Accession GSM2886377 SRX3465423 GSM2886378 SRP126691 SRS2753375 GSM2886378 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886378 GSM2886378 GEO Accession GSM2886378 SRX3465424 GSM2886379 SRP126691 SRS2753372 GSM2886379 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886379 GSM2886379 GEO Accession GSM2886379 SRX3465425 GSM2886380 SRP126691 SRS2753373 GSM2886380 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886380 GSM2886380 GEO Accession GSM2886380 SRX3465426 GSM2886381 SRP126691 SRS2753378 GSM2886381 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886381 GSM2886381 GEO Accession GSM2886381 SRX3465427 GSM2886382 SRP126691 SRS2753374 GSM2886382 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886382 GSM2886382 GEO Accession GSM2886382 SRX3465428 GSM2886383 SRP126691 SRS2753377 GSM2886383 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886383 GSM2886383 GEO Accession GSM2886383 SRX3465429 GSM2886384 SRP126691 SRS2753379 GSM2886384 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886384 GSM2886384 GEO Accession GSM2886384 SRX3465430 GSM2886385 SRP126691 SRS2753376 GSM2886385 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886385 GSM2886385 GEO Accession GSM2886385 SRX3465431 GSM2886386 SRP126691 SRS2753380 GSM2886386 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886386 GSM2886386 GEO Accession GSM2886386 SRX3465432 GSM2886387 SRP126691 SRS2753383 GSM2886387 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886387 GSM2886387 GEO Accession GSM2886387 SRX3465433 GSM2886388 SRP126691 SRS2753381 GSM2886388 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886388 GSM2886388 GEO Accession GSM2886388 SRX3465434 GSM2886389 SRP126691 SRS2753385 GSM2886389 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886389 GSM2886389 GEO Accession GSM2886389 SRX3465435 GSM2886390 SRP126691 SRS2753384 GSM2886390 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886390 GSM2886390 GEO Accession GSM2886390 SRX3465436 GSM2886391 SRP126691 SRS2753382 GSM2886391 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886391 GSM2886391 GEO Accession GSM2886391 SRX3465437 GSM2886392 SRP126691 SRS2753386 GSM2886392 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886392 GSM2886392 GEO Accession GSM2886392 SRX3465438 GSM2886393 SRP126691 SRS2753532 GSM2886393 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886393 GSM2886393 GEO Accession GSM2886393 SRX3465439 GSM2886394 SRP126691 SRS2753387 GSM2886394 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886394 GSM2886394 GEO Accession GSM2886394 SRX3465440 GSM2886395 SRP126691 SRS2753389 GSM2886395 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886395 GSM2886395 GEO Accession GSM2886395 SRX3465441 GSM2886396 SRP126691 SRS2753388 GSM2886396 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886396 GSM2886396 GEO Accession GSM2886396 SRX3465442 GSM2886397 SRP126691 SRS2753393 GSM2886397 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886397 GSM2886397 GEO Accession GSM2886397 SRX3465443 GSM2886398 SRP126691 SRS2753390 GSM2886398 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886398 GSM2886398 GEO Accession GSM2886398 SRX3465444 GSM2886399 SRP126691 SRS2753392 GSM2886399 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886399 GSM2886399 GEO Accession GSM2886399 SRX3465445 GSM2886400 SRP126691 SRS2753391 GSM2886400 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886400 GSM2886400 GEO Accession GSM2886400 SRX3465446 GSM2886401 SRP126691 SRS2753394 GSM2886401 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886401 GSM2886401 GEO Accession GSM2886401 SRX3465447 GSM2886402 SRP126691 SRS2753400 GSM2886402 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886402 GSM2886402 GEO Accession GSM2886402 SRX3465448 GSM2886403 SRP126691 SRS2753399 GSM2886403 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886403 GSM2886403 GEO Accession GSM2886403 SRX3465449 GSM2886404 SRP126691 SRS2753397 GSM2886404 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886404 GSM2886404 GEO Accession GSM2886404 SRX3465450 GSM2886405 SRP126691 SRS2753396 GSM2886405 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886405 GSM2886405 GEO Accession GSM2886405 SRX3465451 GSM2886406 SRP126691 SRS2753395 GSM2886406 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886406 GSM2886406 GEO Accession GSM2886406 SRX3465452 GSM2886407 SRP126691 SRS2753398 GSM2886407 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886407 GSM2886407 GEO Accession GSM2886407 SRX3465453 GSM2886408 SRP126691 SRS2753404 GSM2886408 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886408 GSM2886408 GEO Accession GSM2886408 SRX3465454 GSM2886409 SRP126691 SRS2753401 GSM2886409 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886409 GSM2886409 GEO Accession GSM2886409 SRX3465455 GSM2886410 SRP126691 SRS2753402 GSM2886410 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886410 GSM2886410 GEO Accession GSM2886410 SRX3465456 GSM2886411 SRP126691 SRS2753407 GSM2886411 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886411 GSM2886411 GEO Accession GSM2886411 SRX3465457 GSM2886412 SRP126691 SRS2753405 GSM2886412 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886412 GSM2886412 GEO Accession GSM2886412 SRX3465458 GSM2886413 SRP126691 SRS2753403 GSM2886413 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886413 GSM2886413 GEO Accession GSM2886413 SRX3465459 GSM2886414 SRP126691 SRS2753406 GSM2886414 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886414 GSM2886414 GEO Accession GSM2886414 SRX3465460 GSM2886415 SRP126691 SRS2753409 GSM2886415 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886415 GSM2886415 GEO Accession GSM2886415 SRX3465461 GSM2886416 SRP126691 SRS2753408 GSM2886416 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886416 GSM2886416 GEO Accession GSM2886416 SRX3465462 GSM2886417 SRP126691 SRS2753410 GSM2886417 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886417 GSM2886417 GEO Accession GSM2886417 SRX3465463 GSM2886418 SRP126691 SRS2753412 GSM2886418 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886418 GSM2886418 GEO Accession GSM2886418 SRX3465464 GSM2886419 SRP126691 SRS2753411 GSM2886419 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886419 GSM2886419 GEO Accession GSM2886419 SRX3465465 GSM2886420 SRP126691 SRS2753413 GSM2886420 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886420 GSM2886420 GEO Accession GSM2886420 SRX3465466 GSM2886421 SRP126691 SRS2753414 GSM2886421 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886421 GSM2886421 GEO Accession GSM2886421 SRX3465467 GSM2886422 SRP126691 SRS2753415 GSM2886422 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886422 GSM2886422 GEO Accession GSM2886422 SRX3465468 GSM2886423 SRP126691 SRS2753416 GSM2886423 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886423 GSM2886423 GEO Accession GSM2886423 SRX3465469 GSM2886424 SRP126691 SRS2753417 GSM2886424 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886424 GSM2886424 GEO Accession GSM2886424 SRX3465470 GSM2886425 SRP126691 SRS2753418 GSM2886425 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886425 GSM2886425 GEO Accession GSM2886425 SRX3465471 GSM2886426 SRP126691 SRS2753420 GSM2886426 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886426 GSM2886426 GEO Accession GSM2886426 SRX3465472 GSM2886427 SRP126691 SRS2753419 GSM2886427 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886427 GSM2886427 GEO Accession GSM2886427 SRX3465473 GSM2886428 SRP126691 SRS2753421 GSM2886428 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886428 GSM2886428 GEO Accession GSM2886428 SRX3465474 GSM2886429 SRP126691 SRS2753423 GSM2886429 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886429 GSM2886429 GEO Accession GSM2886429 SRX3465475 GSM2886430 SRP126691 SRS2753424 GSM2886430 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886430 GSM2886430 GEO Accession GSM2886430 SRX3465476 GSM2886431 SRP126691 SRS2753422 GSM2886431 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886431 GSM2886431 GEO Accession GSM2886431 SRX3465477 GSM2886432 SRP126691 SRS2753427 GSM2886432 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886432 GSM2886432 GEO Accession GSM2886432 SRX3465478 GSM2886433 SRP126691 SRS2753425 GSM2886433 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886433 GSM2886433 GEO Accession GSM2886433 SRX3465479 GSM2886434 SRP126691 SRS2753426 GSM2886434 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886434 GSM2886434 GEO Accession GSM2886434 SRX3465480 GSM2886435 SRP126691 SRS2753428 GSM2886435 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886435 GSM2886435 GEO Accession GSM2886435 SRX3465481 GSM2886436 SRP126691 SRS2753430 GSM2886436 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886436 GSM2886436 GEO Accession GSM2886436 SRX3465482 GSM2886437 SRP126691 SRS2753432 GSM2886437 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886437 GSM2886437 GEO Accession GSM2886437 SRX3465483 GSM2886438 SRP126691 SRS2753431 GSM2886438 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886438 GSM2886438 GEO Accession GSM2886438 SRX3465484 GSM2886439 SRP126691 SRS2753429 GSM2886439 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886439 GSM2886439 GEO Accession GSM2886439 SRX3465485 GSM2886440 SRP126691 SRS2753433 GSM2886440 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886440 GSM2886440 GEO Accession GSM2886440 SRX3465486 GSM2886441 SRP126691 SRS2753435 GSM2886441 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886441 GSM2886441 GEO Accession GSM2886441 SRX3465487 GSM2886442 SRP126691 SRS2753434 GSM2886442 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886442 GSM2886442 GEO Accession GSM2886442 SRX3465488 GSM2886443 SRP126691 SRS2753437 GSM2886443 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886443 GSM2886443 GEO Accession GSM2886443 SRX3465489 GSM2886444 SRP126691 SRS2753438 GSM2886444 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886444 GSM2886444 GEO Accession GSM2886444 SRX3465490 GSM2886445 SRP126691 SRS2753441 GSM2886445 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886445 GSM2886445 GEO Accession GSM2886445 SRX3465491 GSM2886446 SRP126691 SRS2753440 GSM2886446 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886446 GSM2886446 GEO Accession GSM2886446 SRX3465492 GSM2886447 SRP126691 SRS2753436 GSM2886447 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886447 GSM2886447 GEO Accession GSM2886447 SRX3465493 GSM2886448 SRP126691 SRS2753439 GSM2886448 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from 1 ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Applied Biosystems/Thermo Fisher Scientific) according to the manufacturer's instructions. Globin RNA was depleted from total RNA (1.5–2 µg) using the human GLOBINclear kit (Thermo Fisher Scientific) according to manufacturer's instructions. RNA yield of total and globin-reduced RNA was assessed using a NanoDrop™ 8000 spectrophotometer (Thermo Fisher Scientific). Quality and integrity of total and globin-reduced RNA were assessed with the HT RNA Assay reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer) and assigned an RNA Quality Score (RQS). Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system), using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired end reads (75 bp) per sample. Illumina HiSeq 4000 gds 302886448 GSM2886448 GEO Accession GSM2886448