SRX3481797 GSM2893780 SRP127006 SRS2767620 GSM2893780 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893780 GSM2893780 GEO Accession GSM2893780 SRX3481798 GSM2893781 SRP127006 SRS2767621 GSM2893781 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893781 GSM2893781 GEO Accession GSM2893781 SRX3481799 GSM2893782 SRP127006 SRS2767622 GSM2893782 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893782 GSM2893782 GEO Accession GSM2893782 SRX3481800 GSM2893783 SRP127006 SRS2767623 GSM2893783 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893783 GSM2893783 GEO Accession GSM2893783 SRX3481801 GSM2893784 SRP127006 SRS2767624 GSM2893784 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893784 GSM2893784 GEO Accession GSM2893784 SRX3481802 GSM2893785 SRP127006 SRS2767625 GSM2893785 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893785 GSM2893785 GEO Accession GSM2893785 SRX3481803 GSM2893786 SRP127006 SRS2767626 GSM2893786 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893786 GSM2893786 GEO Accession GSM2893786 SRX3481804 GSM2893787 SRP127006 SRS2767627 GSM2893787 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893787 GSM2893787 GEO Accession GSM2893787 SRX3481805 GSM2893788 SRP127006 SRS2767628 GSM2893788 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893788 GSM2893788 GEO Accession GSM2893788 SRX3481806 GSM2893789 SRP127006 SRS2767636 GSM2893789 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893789 GSM2893789 GEO Accession GSM2893789 SRX3481807 GSM2893790 SRP127006 SRS2767629 GSM2893790 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893790 GSM2893790 GEO Accession GSM2893790 SRX3481808 GSM2893791 SRP127006 SRS2767630 GSM2893791 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893791 GSM2893791 GEO Accession GSM2893791 SRX3481809 GSM2893792 SRP127006 SRS2767631 GSM2893792 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893792 GSM2893792 GEO Accession GSM2893792 SRX3481810 GSM2893793 SRP127006 SRS2767632 GSM2893793 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893793 GSM2893793 GEO Accession GSM2893793 SRX3481811 GSM2893794 SRP127006 SRS2767633 GSM2893794 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893794 GSM2893794 GEO Accession GSM2893794 SRX3481812 GSM2893795 SRP127006 SRS2767634 GSM2893795 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893795 GSM2893795 GEO Accession GSM2893795 SRX3481813 GSM2893796 SRP127006 SRS2767635 GSM2893796 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893796 GSM2893796 GEO Accession GSM2893796 SRX3481814 GSM2893797 SRP127006 SRS2767637 GSM2893797 ChIP-Seq GENOMIC ChIP For H3K4me3 and H3K364me3 ChIP, nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. For DMC1 ChIP, spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. H3K4me3 and H3K36me3 ChIP libraries were prepared for sequencing using standard Illumina protocols. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles. Illumina HiSeq 2500 gds 302893797 GSM2893797 GEO Accession GSM2893797