GSM2895966: BT-1_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq

SRX3491000 GSM2895966 GSM2895966: BT-1_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774880 GSM2895966 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895966 GSM2895966 GEO Accession GSM2895966 SRX3491001 GSM2895967 GSM2895967: BT-2_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774879 GSM2895967 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895967 GSM2895967 GEO Accession GSM2895967 SRX3491002 GSM2895968 GSM2895968: BT-3_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774881 GSM2895968 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895968 GSM2895968 GEO Accession GSM2895968 SRX3491003 GSM2895969 GSM2895969: BT-4_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774883 GSM2895969 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895969 GSM2895969 GEO Accession GSM2895969 SRX3491004 GSM2895970 GSM2895970: BT-5_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774882 GSM2895970 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895970 GSM2895970 GEO Accession GSM2895970 SRX3491005 GSM2895971 GSM2895971: BT-7_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774884 GSM2895971 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895971 GSM2895971 GEO Accession GSM2895971 SRX3491006 GSM2895972 GSM2895972: BT-8_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774886 GSM2895972 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895972 GSM2895972 GEO Accession GSM2895972 SRX3491007 GSM2895973 GSM2895973: BT-9_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774885 GSM2895973 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895973 GSM2895973 GEO Accession GSM2895973 SRX3491008 GSM2895974 GSM2895974: BT-10_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774887 GSM2895974 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895974 GSM2895974 GEO Accession GSM2895974 SRX3491009 GSM2895975 GSM2895975: BT-11_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774888 GSM2895975 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895975 GSM2895975 GEO Accession GSM2895975 SRX3491010 GSM2895976 GSM2895976: BT-6_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774889 GSM2895976 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895976 GSM2895976 GEO Accession GSM2895976 SRX3491011 GSM2895977 GSM2895977: BT-12_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774890 GSM2895977 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895977 GSM2895977 GEO Accession GSM2895977 SRX3491012 GSM2895978 GSM2895978: BT-13_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774891 GSM2895978 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895978 GSM2895978 GEO Accession GSM2895978 SRX3491013 GSM2895979 GSM2895979: BT-14_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774892 GSM2895979 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895979 GSM2895979 GEO Accession GSM2895979 SRX3491014 GSM2895980 GSM2895980: BT-15_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774893 GSM2895980 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895980 GSM2895980 GEO Accession GSM2895980 SRX3491015 GSM2895981 GSM2895981: BT-16_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774895 GSM2895981 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895981 GSM2895981 GEO Accession GSM2895981 SRX3491016 GSM2895982 GSM2895982: BT-17_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774894 GSM2895982 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895982 GSM2895982 GEO Accession GSM2895982 SRX3491017 GSM2895983 GSM2895983: BT-18_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774897 GSM2895983 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895983 GSM2895983 GEO Accession GSM2895983 SRX3491018 GSM2895984 GSM2895984: BT-19_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774896 GSM2895984 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895984 GSM2895984 GEO Accession GSM2895984 SRX3491019 GSM2895985 GSM2895985: BT-20_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774899 GSM2895985 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895985 GSM2895985 GEO Accession GSM2895985 SRX3491020 GSM2895986 GSM2895986: BT-21_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774898 GSM2895986 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895986 GSM2895986 GEO Accession GSM2895986 SRX3491021 GSM2895987 GSM2895987: BT-22_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774900 GSM2895987 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895987 GSM2895987 GEO Accession GSM2895987 SRX3491022 GSM2895988 GSM2895988: BT-23_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774901 GSM2895988 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895988 GSM2895988 GEO Accession GSM2895988 SRX3491023 GSM2895989 GSM2895989: BT-24_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774906 GSM2895989 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895989 GSM2895989 GEO Accession GSM2895989 SRX3491024 GSM2895990 GSM2895990: BT-25_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774902 GSM2895990 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895990 GSM2895990 GEO Accession GSM2895990 SRX3491025 GSM2895991 GSM2895991: BT-26_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774903 GSM2895991 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895991 GSM2895991 GEO Accession GSM2895991 SRX3491026 GSM2895992 GSM2895992: BT-27_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774907 GSM2895992 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895992 GSM2895992 GEO Accession GSM2895992 SRX3491027 GSM2895993 GSM2895993: BT-28_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774904 GSM2895993 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895993 GSM2895993 GEO Accession GSM2895993 SRX3491028 GSM2895994 GSM2895994: BT-29_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774905 GSM2895994 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 4000 gds 302895994 GSM2895994 GEO Accession GSM2895994 SRX3491029 GSM2895995 GSM2895995: NSF-D_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774909 GSM2895995 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895995 GSM2895995 GEO Accession GSM2895995 SRX3491030 GSM2895996 GSM2895996: NSF-A_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774912 GSM2895996 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895996 GSM2895996 GEO Accession GSM2895996 SRX3491031 GSM2895997 GSM2895997: NSH-D_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774908 GSM2895997 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895997 GSM2895997 GEO Accession GSM2895997 SRX3491032 GSM2895998 GSM2895998: NSH-A_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774910 GSM2895998 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895998 GSM2895998 GEO Accession GSM2895998 SRX3491033 GSM2895999 GSM2895999: NSF-E_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774913 GSM2895999 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302895999 GSM2895999 GEO Accession GSM2895999 SRX3491034 GSM2896000 GSM2896000: NSF-B_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774911 GSM2896000 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896000 GSM2896000 GEO Accession GSM2896000 SRX3491035 GSM2896001 GSM2896001: NSH-E_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774914 GSM2896001 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896001 GSM2896001 GEO Accession GSM2896001 SRX3491036 GSM2896002 GSM2896002: NSH-B_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774915 GSM2896002 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896002 GSM2896002 GEO Accession GSM2896002 SRX3491037 GSM2896003 GSM2896003: NSF-F_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774917 GSM2896003 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896003 GSM2896003 GEO Accession GSM2896003 SRX3491038 GSM2896004 GSM2896004: NSF-C_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774916 GSM2896004 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896004 GSM2896004 GEO Accession GSM2896004 SRX3491039 GSM2896005 GSM2896005: NSH-F_WT-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774919 GSM2896005 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896005 GSM2896005 GEO Accession GSM2896005 SRX3491040 GSM2896006 GSM2896006: NSH-C_K27M-Tag_RNA-seq; Mus musculus; RNA-Seq SRP127264 SRS2774918 GSM2896006 RNA-Seq TRANSCRIPTOMIC cDNA 1 ml of Trizol (Invitrogen, 15596-018) was added to 2 x 106 dissociated neurosphere cells. 2 ul of 1:20 diluted ERCC spike in (Ambion Cat #4456740, Lot # 1412017) was added directly to the Trizol. For tumor tissue, a piece of snap frozen tissue was mechanically dissociated in Trizol. RNA was extracted following the standard Trizol protocol except the final 70% EtOH wash was repeated three times. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina.) Illumina HiSeq 2500 gds 302896006 GSM2896006 GEO Accession GSM2896006