SRX3504433 Ac_UNTN_42028_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781987 SAMN08214222 Ac_UNTN_42028_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504434 Ac_Camp_27004_5 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781988 SAMN08214221 Ac_Camp_27004_5 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504435 Ac_Camp_27004_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781989 SAMN08214220 Ac_Camp_27004_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504436 Ac_Camp_27004_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781990 SAMN08214219 Ac_Camp_27004_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504437 Av_SFWB_42074_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781991 SAMN08214226 Av_SFWB_42074_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504438 Av_SFWB_42074_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781993 SAMN08214225 Av_SFWB_42074_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504439 Ac_UNTN_42028_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781992 SAMN08214224 Ac_UNTN_42028_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504440 Ac_UNTN_42028_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781994 SAMN08214223 Ac_UNTN_42028_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504441 Sc_Croo_17006_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781995 SAMN08214228 Sc_Croo_17006_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504442 Av_SFWB_42074_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781996 SAMN08214227 Av_SFWB_42074_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504443 Sc_Dutc_18068_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781998 SAMN08214236 Sc_Dutc_18068_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504444 Sc_Dutc_18068_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781997 SAMN08214235 Sc_Dutc_18068_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504445 Sc_Dutc_18068_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2781999 SAMN08214234 Sc_Dutc_18068_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504446 Sc_Dixo_17010_5 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782000 SAMN08214233 Sc_Dixo_17010_5 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504447 Sc_Dixo_17010_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782001 SAMN08214232 Sc_Dixo_17010_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504448 Sc_Dixo_17010_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782002 SAMN08214231 Sc_Dixo_17010_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504449 Sc_Dixo_17010_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782003 SAMN08214230 Sc_Dixo_17010_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504450 Sc_Croo_17006_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782004 SAMN08214229 Sc_Croo_17006_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504451 Sv_Deer_17008_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782005 SAMN08214250 Sv_Deer_17008_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504452 Sv_Deer_17008_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782006 SAMN08214249 Sv_Deer_17008_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504453 Sv_Deer_17008_5 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782007 SAMN08214252 Sv_Deer_17008_5 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504454 Sv_Deer_17008_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782008 SAMN08214251 Sv_Deer_17008_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504455 Sv_Ston_17021_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782011 SAMN08214254 Sv_Ston_17021_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504456 Sv_Ston_17021_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782009 SAMN08214253 Sv_Ston_17021_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504457 Sc_Mocc_18073_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782010 SAMN08214238 Sc_Mocc_18073_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504458 Sc_Mocc_18073_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782012 SAMN08214237 Sc_Mocc_18073_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504459 Sv_Ston_17021_5 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782013 SAMN08214256 Sv_Ston_17021_5 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504460 Sv_Ston_17021_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782014 SAMN08214255 Sv_Ston_17021_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504461 Sv_Cold_17005_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782016 SAMN08214247 Sv_Cold_17005_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504462 Sv_Deer_17008_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782015 SAMN08214248 Sv_Deer_17008_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504463 Sc_Mocc_18073_5 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782017 SAMN08214241 Sc_Mocc_18073_5 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504464 Sc_UNTW_42016_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782018 SAMN08214242 Sc_UNTW_42016_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504465 Sc_Mocc_18073_3 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782019 SAMN08214239 Sc_Mocc_18073_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504466 Sc_Mocc_18073_4 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782021 SAMN08214240 Sc_Mocc_18073_4 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504467 Sc_Wist_18069_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782020 SAMN08214245 Sc_Wist_18069_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504468 Sv_Cold_17005_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782022 SAMN08214246 Sv_Cold_17005_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504469 Sc_UNTW_42016_2 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782023 SAMN08214243 Sc_UNTW_42016_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX3504470 Sc_Wist_18069_1 SRP127387 PRJNA423063 Total RNA was isolated using standard methods. RNA was polyA selected to enrich for mRNA, then fragmented chemically. After the mRNA was fragmented, cDNA was synthesized from the mRNA by reverse transcription (RT). Platform-specific RT primers, designed with a random 3-nucleotide barcode, anneal to the fragmented mRNA and extend to the 5 end of the mRNA. The SMARTScribe RT enzyme inserts a non-templated CCC sequence to the 3 end of the first synthesized strand. This allows the SMART7.5 strand-switching primer s GGG sequence to anneal to the CCC sequence, which allows extension of the template for the first synthesized cDNA strand to include PCR primer sequences. AMPure bead purification was used to separate the primers from the cDNA. The cDNA was subsequently amplified for a small number of cycles by PCR and products of the desired size range (400-500 bp) purified on agarose gels. SRS2782024 SAMN08214244 Sc_Wist_18069_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500