SRX3516446 T16.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785120 SAMN08197056 T16.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516447 T16.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785120 SAMN08197056 T16.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516448 T15.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785121 SAMN08197055 T15.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516449 T16.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785120 SAMN08197056 T16.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516450 T15.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785121 SAMN08197055 T15.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516451 T15.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785121 SAMN08197055 T15.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516452 T14.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785114 SAMN08197054 T14.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516453 T14.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785114 SAMN08197054 T14.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516454 T17.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785119 SAMN08197057 T17.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516455 T17.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785119 SAMN08197057 T17.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516456 T12.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785117 SAMN08197052 T12.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516457 T12.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785117 SAMN08197052 T12.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516458 T13.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785115 SAMN08197053 T13.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516459 T13.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785115 SAMN08197053 T13.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516460 T11.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785116 SAMN08197051 T11.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516461 T11.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785116 SAMN08197051 T11.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516462 T11.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785116 SAMN08197051 T11.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516463 T12.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785117 SAMN08197052 T12.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516464 T13.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785115 SAMN08197053 T13.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516465 T14.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785114 SAMN08197054 T14.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516466 T18.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785118 SAMN08197058 T18.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516467 T18.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785118 SAMN08197058 T18.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516468 T18.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785118 SAMN08197058 T18.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516469 T17.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785119 SAMN08197057 T17.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516470 T20.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785123 SAMN08197060 T20.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516471 T19.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785122 SAMN08197059 T19.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516472 T19.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785122 SAMN08197059 T19.2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516473 T19.1 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785122 SAMN08197059 T19.1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516474 T20.3 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785123 SAMN08197060 T20.3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3516475 T20.2 SRP127476 PRJNA422681 Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94 C for 10 min; (2) 25 cycles of further denaturation at 94 C for 45 s, annealing at 53 C for 45 s, and extension at 72 C for 1 min; (3) concluding extension at 72 C for 10 min. The PCR mixture contained 1.25 ?L DMSO. SRS2785123 SAMN08197060 T20.2 AMPLICON METAGENOMIC PCR Illumina MiSeq