SRX3549422 GSM2928425 SRP128723 SRS2823392 GSM2928425 ChIP-Seq GENOMIC ChIP NS-5 cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde to cross-link chromatin. Chromatin was prepared for sonication with 20 mM Tris-HCl pH8, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100. We used 15 cycles of 30 seconds ON, 30 seconds OFF on a Bioruptor Pico sonication device (Diagenode Cat# B01060001) to shear chromatin to 150-200bp fragments. The resulting chromatin extract was incubated overnight at 4°C with 100ul of Dynal Protein G magnetic beads that had been pre-incubated with approximately 10 μg of the appropriate antibody. Beads were washed 1X with the sonication buffer, 1X with 20 mM Tris-HCl pH8, 500 mM NaCl, 2 mM EDTA, 0.1% SDS, 1%Triton X-100, 1X with 10 mM Tris-HCl pH8, 250 mM LiCl, 2 mM EDTA, 1% NP40 and 1X with TE containing 50 mM NaCl. Bound complexes were eluted from the beads (50 mM Tris-Hcl, pH 8.0, 10 mM EDTA and 1% SDS) by heating at 65°C for 1 hr with occasional vortexing and crosslinking was reversed by overnight incubation at 65°C. Finally ,DNA was precipitated using phenol:chloroform:isoamyl alcohol (PCI). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit Illumina HiSeq 2000 gds 302928425 GSM2928425 GEO Accession GSM2928425 SRX3549423 GSM2928426 SRP128723 SRS2823393 GSM2928426 ChIP-Seq GENOMIC ChIP NS-5 cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde to cross-link chromatin. Chromatin was prepared for sonication with 20 mM Tris-HCl pH8, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100. We used 15 cycles of 30 seconds ON, 30 seconds OFF on a Bioruptor Pico sonication device (Diagenode Cat# B01060001) to shear chromatin to 150-200bp fragments. The resulting chromatin extract was incubated overnight at 4°C with 100ul of Dynal Protein G magnetic beads that had been pre-incubated with approximately 10 μg of the appropriate antibody. Beads were washed 1X with the sonication buffer, 1X with 20 mM Tris-HCl pH8, 500 mM NaCl, 2 mM EDTA, 0.1% SDS, 1%Triton X-100, 1X with 10 mM Tris-HCl pH8, 250 mM LiCl, 2 mM EDTA, 1% NP40 and 1X with TE containing 50 mM NaCl. Bound complexes were eluted from the beads (50 mM Tris-Hcl, pH 8.0, 10 mM EDTA and 1% SDS) by heating at 65°C for 1 hr with occasional vortexing and crosslinking was reversed by overnight incubation at 65°C. Finally ,DNA was precipitated using phenol:chloroform:isoamyl alcohol (PCI). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit Illumina HiSeq 2000 gds 302928426 GSM2928426 GEO Accession GSM2928426 SRX3549424 GSM2928427 SRP128723 SRS2823394 GSM2928427 ChIP-Seq GENOMIC ChIP NS-5 cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde to cross-link chromatin. Chromatin was prepared for sonication with 20 mM Tris-HCl pH8, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100. We used 15 cycles of 30 seconds ON, 30 seconds OFF on a Bioruptor Pico sonication device (Diagenode Cat# B01060001) to shear chromatin to 150-200bp fragments. The resulting chromatin extract was incubated overnight at 4°C with 100ul of Dynal Protein G magnetic beads that had been pre-incubated with approximately 10 μg of the appropriate antibody. Beads were washed 1X with the sonication buffer, 1X with 20 mM Tris-HCl pH8, 500 mM NaCl, 2 mM EDTA, 0.1% SDS, 1%Triton X-100, 1X with 10 mM Tris-HCl pH8, 250 mM LiCl, 2 mM EDTA, 1% NP40 and 1X with TE containing 50 mM NaCl. Bound complexes were eluted from the beads (50 mM Tris-Hcl, pH 8.0, 10 mM EDTA and 1% SDS) by heating at 65°C for 1 hr with occasional vortexing and crosslinking was reversed by overnight incubation at 65°C. Finally ,DNA was precipitated using phenol:chloroform:isoamyl alcohol (PCI). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit Illumina HiSeq 2000 gds 302928427 GSM2928427 GEO Accession GSM2928427 SRX3549425 GSM2928428 SRP128723 SRS2823397 GSM2928428 ChIP-Seq GENOMIC ChIP NS-5 cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde to cross-link chromatin. Chromatin was prepared for sonication with 20 mM Tris-HCl pH8, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100. We used 15 cycles of 30 seconds ON, 30 seconds OFF on a Bioruptor Pico sonication device (Diagenode Cat# B01060001) to shear chromatin to 150-200bp fragments. The resulting chromatin extract was incubated overnight at 4°C with 100ul of Dynal Protein G magnetic beads that had been pre-incubated with approximately 10 μg of the appropriate antibody. Beads were washed 1X with the sonication buffer, 1X with 20 mM Tris-HCl pH8, 500 mM NaCl, 2 mM EDTA, 0.1% SDS, 1%Triton X-100, 1X with 10 mM Tris-HCl pH8, 250 mM LiCl, 2 mM EDTA, 1% NP40 and 1X with TE containing 50 mM NaCl. Bound complexes were eluted from the beads (50 mM Tris-Hcl, pH 8.0, 10 mM EDTA and 1% SDS) by heating at 65°C for 1 hr with occasional vortexing and crosslinking was reversed by overnight incubation at 65°C. Finally ,DNA was precipitated using phenol:chloroform:isoamyl alcohol (PCI). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit Illumina HiSeq 2000 gds 302928428 GSM2928428 GEO Accession GSM2928428