SRX3565011 SO_5329 SRP129756 PRJNA430159 Small RNA libraries for sequencing were constructed according to the Illumina TruSeq Small RNA library protocol outlined in TruSeq Small RNA Sample Preparation Guide (Part # 15004197; Rev. G; January 2015). 1 g of Total RNA was used as starting material. Briefly, 3' adaptors were ligated to the specific 3' OH group of microRNAs followed by 5' adaptor ligation. The ligated products were reverse transcribed with Superscript III reverse transcriptase by priming with reverse transcriptase primers. The cDNA was enriched and barcoded by PCR (15 cycles) and separated on polyacrylamide gel. The libraries were size selected in the range of 140 bp 160 bp followed by overnight gel elution and salt precipitation using Glycogen, 3 M Sodium Acetate and absolute ethanol. The precipitate was re-suspended in Nuclease free water. The prepared libraries were quantified using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). The resulting cDNAs were sequenced using Illumina NextSeq500 75*1 sequencing platform. SRS2838618 SAMN08366959 SO_5329 miRNA-Seq TRANSCRIPTOMIC PCR NextSeq 500