SRX3641596 T8 48h SRP132029 bp0 Total RNA was isolated from the frozen seedlings of tartary buckwheat using an RNeasy Mini Kit (Qiagen, Valencia, USA) and cleaned by ethanol precipitation. We removed the rRNAs in the total RNA using a ribo-zero rRNA removal kit (Epicentre, RZPL11016) and constructed a cDNA library for RNA sequencing using a TruSeq stranded total RNA sample prep kit-LT set A and B (Illumina, RS-122-2301 and 2302) according to the manufacturer s instructions (Illumina, San Diego, CA, USA). The cDNA library was sequenced in 76 bp length paired-end (PE) reads in an Illumina NextSeq500 sequencer (Illumina Inc., San Diego, CA, USA) to produce 69,570,892 raw sequencing reads. SRS2907778 Tartary buckwheat T8 48h RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX3641597 T8 24h SRP132029 bp0 Total RNA was isolated from the frozen seedlings of tartary buckwheat using an RNeasy Mini Kit (Qiagen, Valencia, USA) and cleaned by ethanol precipitation. We removed the rRNAs in the total RNA using a ribo-zero rRNA removal kit (Epicentre, RZPL11016) and constructed a cDNA library for RNA sequencing using a TruSeq stranded total RNA sample prep kit-LT set A and B (Illumina, RS-122-2301 and 2302) according to the manufacturer s instructions (Illumina, San Diego, CA, USA). The cDNA library was sequenced in 76 bp length paired-end (PE) reads in an Illumina NextSeq500 sequencer (Illumina Inc., San Diego, CA, USA) to produce 69,570,892 raw sequencing reads. SRS2907778 Tartary buckwheat T8 24h RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX3641598 T8 4h SRP132029 bp0 Total RNA was isolated from the frozen seedlings of tartary buckwheat using an RNeasy Mini Kit (Qiagen, Valencia, USA) and cleaned by ethanol precipitation. We removed the rRNAs in the total RNA using a ribo-zero rRNA removal kit (Epicentre, RZPL11016) and constructed a cDNA library for RNA sequencing using a TruSeq stranded total RNA sample prep kit-LT set A and B (Illumina, RS-122-2301 and 2302) according to the manufacturer s instructions (Illumina, San Diego, CA, USA). The cDNA library was sequenced in 76 bp length paired-end (PE) reads in an Illumina NextSeq500 sequencer (Illumina Inc., San Diego, CA, USA) to produce 69,570,892 raw sequencing reads. SRS2907778 Tartary buckwheat T8 4h RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX3641599 T8 0h SRP132029 bp0 Total RNA was isolated from the frozen seedlings of tartary buckwheat using an RNeasy Mini Kit (Qiagen, Valencia, USA) and cleaned by ethanol precipitation. We removed the rRNAs in the total RNA using a ribo-zero rRNA removal kit (Epicentre, RZPL11016) and constructed a cDNA library for RNA sequencing using a TruSeq stranded total RNA sample prep kit-LT set A and B (Illumina, RS-122-2301 and 2302) according to the manufacturer s instructions (Illumina, San Diego, CA, USA). The cDNA library was sequenced in 76 bp length paired-end (PE) reads in an Illumina NextSeq500 sequencer (Illumina Inc., San Diego, CA, USA) to produce 69,570,892 raw sequencing reads. SRS2907778 Tartary buckwheat T8 0h RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500