SRX3643081 D1 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908066 SAMN08450010 D1 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643082 D2 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908067 SAMN08450011 D2 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643083 C1 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908068 SAMN08450098 C1 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643084 C2 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908069 SAMN08450099 C2 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643085 B1 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908070 SAMN08450096 B1 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643086 B2 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908071 SAMN08450097 B2 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643087 A1 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908072 SAMN08450094 A1 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500 SRX3643088 A2 SRP132048 PRJNA432483 16S rRNA was partially amplified using the forward primer 515F (5?-GTGCCAGCMGCCGCGG-3?) and the reverse primer 907R (5?-CCGTCAATTCCTTTGAGTTT-3?).19 The PCR reaction program was as follows: 0.2? ?of each primer and 0.2?mM dNTP, and 1.5?mM MgCl2 and 0.2?units of Taq polymerase. PCR amplification (initial denaturation at 95? C for 2?min, and 25 cycles at 95? C for 30?s, 55? C for 30?s and 72? C for 30?s, and a final extension at 72? C for 5?min) and gel purification procedures were conducted SRS2908073 SAMN08450095 A2 miRNA-Seq METAGENOMIC PCR Illumina HiSeq 2500