SRX3741065 GSM3021797 SRP133438 SRS2997737 GSM3021797 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021797 GSM3021797 GEO Accession GSM3021797 SRX3741066 GSM3021798 SRP133438 SRS2997738 GSM3021798 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021798 GSM3021798 GEO Accession GSM3021798 SRX3741067 GSM3021799 SRP133438 SRS2997739 GSM3021799 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021799 GSM3021799 GEO Accession GSM3021799 SRX3741068 GSM3021800 SRP133438 SRS2997740 GSM3021800 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021800 GSM3021800 GEO Accession GSM3021800 SRX3741069 GSM3021801 SRP133438 SRS2997741 GSM3021801 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021801 GSM3021801 GEO Accession GSM3021801 SRX3741070 GSM3021802 SRP133438 SRS2997742 GSM3021802 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021802 GSM3021802 GEO Accession GSM3021802 SRX3741071 GSM3021803 SRP133438 SRS2997743 GSM3021803 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021803 GSM3021803 GEO Accession GSM3021803 SRX3741072 GSM3021804 SRP133438 SRS2997744 GSM3021804 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021804 GSM3021804 GEO Accession GSM3021804 SRX3741073 GSM3021805 SRP133438 SRS2997745 GSM3021805 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021805 GSM3021805 GEO Accession GSM3021805 SRX3741074 GSM3021806 SRP133438 SRS2997746 GSM3021806 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021806 GSM3021806 GEO Accession GSM3021806 SRX3741075 GSM3021807 SRP133438 SRS2997747 GSM3021807 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021807 GSM3021807 GEO Accession GSM3021807 SRX3741076 GSM3021808 SRP133438 SRS2997748 GSM3021808 RNA-Seq TRANSCRIPTOMIC cDNA Human organoid samples: Pools of 5-10 organoids per replicate were harvested in TRIzol at day 28 for RNA extraction. Mouse organoid samples: Three pools of 16 organoids were isolated in TRIzol after 6 days of differentiation for EV and NOTCH2NL-Sh_T197I organoids. RNA was isolated using standard TRIzol protocol, followed by DNaseI treatment (Rochte) and column purification (Zymo RNA clean and concentrator 5). mRNA was isolated from total RNA using polyA selection Dynabeads mRNA DIRECT Micro Purification Kit (Thermofisher). Library was prepared using strand-specific Ion Total RNA-seq Kit v2 (Thermofisher) and Ion Xpress RNA-seq Barcode 1-16 (Thermofisher) to label different samples. Ion Torrent Proton gds 303021808 GSM3021808 GEO Accession GSM3021808