SRX3782462 GSM3037914 SRP134957 SRS3035742 GSM3037914 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037914 GSM3037914 GEO Accession GSM3037914 SRX3782463 GSM3037915 SRP134957 SRS3035741 GSM3037915 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037915 GSM3037915 GEO Accession GSM3037915 SRX3782464 GSM3037916 SRP134957 SRS3035743 GSM3037916 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037916 GSM3037916 GEO Accession GSM3037916 SRX3782465 GSM3037917 SRP134957 SRS3035744 GSM3037917 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037917 GSM3037917 GEO Accession GSM3037917 SRX3782466 GSM3037918 SRP134957 SRS3035745 GSM3037918 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037918 GSM3037918 GEO Accession GSM3037918 SRX3782467 GSM3037919 SRP134957 SRS3035746 GSM3037919 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037919 GSM3037919 GEO Accession GSM3037919 SRX3782468 GSM3037920 SRP134957 SRS3035747 GSM3037920 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037920 GSM3037920 GEO Accession GSM3037920 SRX3782469 GSM3037921 SRP134957 SRS3035748 GSM3037921 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037921 GSM3037921 GEO Accession GSM3037921 SRX3782470 GSM3037922 SRP134957 SRS3035749 GSM3037922 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037922 GSM3037922 GEO Accession GSM3037922 SRX3782471 GSM3037923 SRP134957 SRS3035750 GSM3037923 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037923 GSM3037923 GEO Accession GSM3037923 SRX3782472 GSM3037924 SRP134957 SRS3035752 GSM3037924 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037924 GSM3037924 GEO Accession GSM3037924 SRX3782473 GSM3037925 SRP134957 SRS3035751 GSM3037925 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037925 GSM3037925 GEO Accession GSM3037925 SRX3782474 GSM3037926 SRP134957 SRS3035753 GSM3037926 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037926 GSM3037926 GEO Accession GSM3037926 SRX3782475 GSM3037927 SRP134957 SRS3035754 GSM3037927 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037927 GSM3037927 GEO Accession GSM3037927 SRX3782476 GSM3037928 SRP134957 SRS3035755 GSM3037928 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037928 GSM3037928 GEO Accession GSM3037928 SRX3782477 GSM3037929 SRP134957 SRS3035757 GSM3037929 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037929 GSM3037929 GEO Accession GSM3037929 SRX3782478 GSM3037930 SRP134957 SRS3035756 GSM3037930 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037930 GSM3037930 GEO Accession GSM3037930 SRX3782479 GSM3037931 SRP134957 SRS3035758 GSM3037931 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037931 GSM3037931 GEO Accession GSM3037931 SRX3782480 GSM3037932 SRP134957 SRS3035759 GSM3037932 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037932 GSM3037932 GEO Accession GSM3037932 SRX3782481 GSM3037933 SRP134957 SRS3035760 GSM3037933 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037933 GSM3037933 GEO Accession GSM3037933 SRX3782482 GSM3037934 SRP134957 SRS3035761 GSM3037934 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037934 GSM3037934 GEO Accession GSM3037934 SRX3782483 GSM3037935 SRP134957 SRS3035762 GSM3037935 ChIP-Seq GENOMIC ChIP ChIP was performed as described previously (PMID: 27425608). Briefly, cells were fixed with 1% formaldehyde for 10min, quenched with 0.125M glycine for 5min and washed with ice cold PBS containing protease inhibitor. Cell pellets were snap frozen in liquid nitrogen and saved in -80oC. Cells were lysed in lysis buffer as described here PMID: 27425608 and chromatin was sheared using Diagenode Bioruptor plus (16 min, 30 sec on/30 sec off, setting high). Immunoprecipitation was performed using 25 million cells for each H3k27Ac (Abcam, ab4729, 5µg per IP) and RNA Pol II (Biolegend 920102 Clone 8WG16, 5µl per IP). 75 million cells and 5µg antibody was used for Brd4 IP (Bethyl A301-985A). IP Samples were reverse cross linked and DNA was purified using phenol:chloroform:isoamyl alcohol. Purified DNA samples were sent to WIGTC for NGS library preparation and sequencing. TruSeq ChIP library preparation kit was used according to manufacturer's instructions (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html) ChIP-Seq with single end 40bp sequencing Illumina HiSeq 2500 gds 303037935 GSM3037935 GEO Accession GSM3037935