SRX3823722 ABR_Inf_Cold SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073120 GV0363 ABR_Inf_Cold AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823723 ABR_C2_Cold_BioRep SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073121 GV0361 ABR_C2_Cold_BioRep AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823724 ABR_C3_Cold_BioRep SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073123 GV0362 ABR_C3_Cold_BioRep AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823725 ABR_C1_Warm SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073122 GV0351 ABR_C1_Warm AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823726 ABR_C2_Warm SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073124 GV0352 ABR_C2_Warm AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823727 ABR_C2_Warm_BioRep SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073125 GV0353 ABR_C2_Warm_BioRep AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823728 ABR_C3_Warm SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073126 GV0354 ABR_C3_Warm AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823729 ABR_C3_Warm_BioRep SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073127 GV0355 ABR_C3_Warm_BioRep AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823730 ABR_Inf_Warm SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073128 GV0356 ABR_Inf_Warm AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823731 ABR_Inf_Warm_BioRep SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073129 GV0357 ABR_Inf_Warm_BioRep AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823732 ABR_C2_Cold SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073130 GV0358 ABR_C2_Cold AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823733 ABR_C1_Cold SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073131 GV0359 ABR_C1_Cold AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3823734 ABR_C3_Cold SRP136078 bp0 Genomic DNA was extracted from 2.0 ml of anaerobic sludge using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer s protocol and stored at -80 C. DNA was quantified using a Qubit Fluorometer and a Qubit dsDNA High Sensitivity Assay Kit (Thermo-Fisher, Inc.). DNA samples were amplified using primers 515F (5 GTGCCAGCMGCCGCGGTAA3 ) and 806R (5 GGACTACHVGGGTWTCTAAT3 ) following the two-step amplification and barcoding strategy described in Stamps et al. (2016). SRS3073132 GV0360 ABR_C3_Cold AMPLICON METAGENOMIC PCR Illumina MiSeq