SRX3824072 Tricolor_green_layer SRP136096 bp0 100 ng of metagenomic DNA was fragmented to 200 300 bp by sonication with the Covaris S220 System (Covaris, Woburn, Massachusetts, USA). The Ion Xpress Plus Fragment Library Kit and the Ion Xpress Barcode Adapters 1 16 Kit (Life Technologies) was then used to prepare a barcoded shotgun library. The Ion PGM Template OT2 200 Kit (Life Technologies) was used for emulsion PCR. Sequencing was performed with the genomic analyser Ion Torrent PGM (Life Technologies) using Ion 318 Chip Kit v2 and the Ion PGM Sequencing 200 Kit v2 (Life Technologies). SRS3073353 Trekhsvetnoe lake metagenome Tricolor_green_layer WGS METAGENOMIC size fractionation Ion Torrent PGM