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  <EXPERIMENT accession="SRX3887004" alias="Bare_Fallow">
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      <PRIMARY_ID>SRX3887004</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB3867557">Bare_Fallow</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Metogenome Barefallow</TITLE>
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        <PRIMARY_ID>SRP137590</PRIMARY_ID>
        <SUBMITTER_ID namespace="SUB3867557">bp0</SUBMITTER_ID>
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      <DESIGN_DESCRIPTION>After a treatment of the sample, the DNA extraction was made with the Powermax soil DNA isolation kit following the manufacturers instructions. The purity was made using a Nanodrop ND-1000 spectrophotometer. The DNA concentration was determined using a Qubit 2.0 flurometer and dsDNA BR Assay kit (Invitrogen ). The integrity of the DNA was confirmed by electrophoresis in a 0.8 % agarose gel with 1 X TAE buffer. Sequencing was done on the Illumina MiSeq  platform at the High-throughput Genome Analysis Core (HGAC). Fungal ITS1 region was amplified using primers ITS1F (5 -CTTGGCCATTTAGAGGAAGTAA-3 ) and ITS2 (5 -GCTGCGTTCTTCATCGATGC-3 ) using the method described by Smith &amp; Peay (2014). The 16S bioinformatics pipeline was applied, recommended by the Brazilian Microbiome Project available at http://www.brmicrobiome.org/its-profiling-new-illumina (Pylro et al., 2014) that apply Qiime and Usearch 7.0 for filtering, clustering and diversity analysis.</DESIGN_DESCRIPTION>
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          <PRIMARY_ID>SRS3125557</PRIMARY_ID>
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        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
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          <PAIRED/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX3887005" alias="Grassland">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX3887005</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB3867557">Grassland</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Metogenome Grassland</TITLE>
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        <PRIMARY_ID>SRP137590</PRIMARY_ID>
        <SUBMITTER_ID namespace="SUB3867557">bp0</SUBMITTER_ID>
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    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>After a treatment of the sample, the DNA extraction was made with the Powermax soil DNA isolation kit following the manufacturers instructions. The purity was made using a Nanodrop ND-1000 spectrophotometer. The DNA concentration was determined using a Qubit 2.0 flurometer and dsDNA BR Assay kit (Invitrogen ). The integrity of the DNA was confirmed by electrophoresis in a 0.8 % agarose gel with 1 X TAE buffer. Sequencing was done on the Illumina MiSeq  platform at the High-throughput Genome Analysis Core (HGAC). Fungal ITS1 region was amplified using primers ITS1F (5 -CTTGGCCATTTAGAGGAAGTAA-3 ) and ITS2 (5 -GCTGCGTTCTTCATCGATGC-3 ) using the method described by Smith &amp; Peay (2014). The 16S bioinformatics pipeline was applied, recommended by the Brazilian Microbiome Project available at http://www.brmicrobiome.org/its-profiling-new-illumina (Pylro et al., 2014) that apply Qiime and Usearch 7.0 for filtering, clustering and diversity analysis.</DESIGN_DESCRIPTION>
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          <PRIMARY_ID>SRS3125558</PRIMARY_ID>
          <SUBMITTER_ID namespace="pda|elcioneto">G</SUBMITTER_ID>
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        <LIBRARY_NAME>Grassland</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
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          <PAIRED/>
        </LIBRARY_LAYOUT>
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    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX3887006" alias="Trindade Island">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX3887006</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB3867557">Trindade Island</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Metogenome Trindade Island</TITLE>
    <STUDY_REF accession="SRP137590">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP137590</PRIMARY_ID>
        <SUBMITTER_ID namespace="SUB3867557">bp0</SUBMITTER_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>After a treatment of the sample, the DNA extraction was made with the Powermax soil DNA isolation kit following the manufacturers instructions. The purity was made using a Nanodrop ND-1000 spectrophotometer. The DNA concentration was determined using a Qubit 2.0 flurometer and dsDNA BR Assay kit (Invitrogen ). The integrity of the DNA was confirmed by electrophoresis in a 0.8 % agarose gel with 1 X TAE buffer. Sequencing was done on the Illumina MiSeq  platform at the High-throughput Genome Analysis Core (HGAC). Fungal ITS1 region was amplified using primers ITS1F (5 -CTTGGCCATTTAGAGGAAGTAA-3 ) and ITS2 (5 -GCTGCGTTCTTCATCGATGC-3 ) using the method described by Smith &amp; Peay (2014). The 16S bioinformatics pipeline was applied, recommended by the Brazilian Microbiome Project available at http://www.brmicrobiome.org/its-profiling-new-illumina (Pylro et al., 2014) that apply Qiime and Usearch 7.0 for filtering, clustering and diversity analysis.</DESIGN_DESCRIPTION>
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          <PRIMARY_ID>SRS3125559</PRIMARY_ID>
          <SUBMITTER_ID namespace="pda|elcioneto">T</SUBMITTER_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>Trindade Island</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
</EXPERIMENT_SET>
