SRX3889781 GSM3081986 SRP137692 SRS3127636 GSM3081986 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081986 GSM3081986 GEO Accession GSM3081986 SRX3889782 GSM3081987 SRP137692 SRS3127635 GSM3081987 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081987 GSM3081987 GEO Accession GSM3081987 SRX3889783 GSM3081988 SRP137692 SRS3127634 GSM3081988 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081988 GSM3081988 GEO Accession GSM3081988 SRX3889784 GSM3081989 SRP137692 SRS3127637 GSM3081989 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081989 GSM3081989 GEO Accession GSM3081989 SRX3889785 GSM3081990 SRP137692 SRS3127638 GSM3081990 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081990 GSM3081990 GEO Accession GSM3081990 SRX3889786 GSM3081991 SRP137692 SRS3127639 GSM3081991 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081991 GSM3081991 GEO Accession GSM3081991 SRX3889787 GSM3081992 SRP137692 SRS3127640 GSM3081992 ATAC-seq GENOMIC other Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081992 GSM3081992 GEO Accession GSM3081992 SRX3889788 GSM3081993 SRP137692 SRS3127641 GSM3081993 ATAC-seq GENOMIC other Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081993 GSM3081993 GEO Accession GSM3081993 SRX3889789 GSM3081994 SRP137692 SRS3127642 GSM3081994 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081994 GSM3081994 GEO Accession GSM3081994 SRX3889790 GSM3081995 SRP137692 SRS3127643 GSM3081995 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081995 GSM3081995 GEO Accession GSM3081995 SRX3889791 GSM3081996 SRP137692 SRS3127644 GSM3081996 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081996 GSM3081996 GEO Accession GSM3081996 SRX3889792 GSM3081997 SRP137692 SRS3127645 GSM3081997 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303081997 GSM3081997 GEO Accession GSM3081997 SRX3889809 GSM3082014 SRP137692 SRS3127662 GSM3082014 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082014 GSM3082014 GEO Accession GSM3082014 SRX3889810 GSM3082015 SRP137692 SRS3127663 GSM3082015 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082015 GSM3082015 GEO Accession GSM3082015 SRX3889811 GSM3082016 SRP137692 SRS3127664 GSM3082016 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082016 GSM3082016 GEO Accession GSM3082016 SRX3889812 GSM3082017 SRP137692 SRS3127665 GSM3082017 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082017 GSM3082017 GEO Accession GSM3082017 SRX3889813 GSM3082018 SRP137692 SRS3127666 GSM3082018 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082018 GSM3082018 GEO Accession GSM3082018 SRX3889814 GSM3082019 SRP137692 SRS3127667 GSM3082019 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082019 GSM3082019 GEO Accession GSM3082019 SRX3889815 GSM3082020 SRP137692 SRS3127669 GSM3082020 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082020 GSM3082020 GEO Accession GSM3082020 SRX3889816 GSM3082021 SRP137692 SRS3127668 GSM3082021 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082021 GSM3082021 GEO Accession GSM3082021 SRX3889819 GSM3082024 SRP137692 SRS3127672 GSM3082024 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082024 GSM3082024 GEO Accession GSM3082024 SRX3889820 GSM3082025 SRP137692 SRS3127673 GSM3082025 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082025 GSM3082025 GEO Accession GSM3082025 SRX3889821 GSM3082026 SRP137692 SRS3127675 GSM3082026 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082026 GSM3082026 GEO Accession GSM3082026 SRX3889822 GSM3082027 SRP137692 SRS3127677 GSM3082027 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082027 GSM3082027 GEO Accession GSM3082027 SRX3889823 GSM3082028 SRP137692 SRS3127674 GSM3082028 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082028 GSM3082028 GEO Accession GSM3082028 SRX3889824 GSM3082029 SRP137692 SRS3127676 GSM3082029 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082029 GSM3082029 GEO Accession GSM3082029 SRX3889825 GSM3082030 SRP137692 SRS3127678 GSM3082030 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082030 GSM3082030 GEO Accession GSM3082030 SRX3889826 GSM3082031 SRP137692 SRS3127679 GSM3082031 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082031 GSM3082031 GEO Accession GSM3082031 SRX3889827 GSM3082032 SRP137692 SRS3127680 GSM3082032 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082032 GSM3082032 GEO Accession GSM3082032 SRX3889828 GSM3082033 SRP137692 SRS3127681 GSM3082033 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082033 GSM3082033 GEO Accession GSM3082033 SRX3889829 GSM3082034 SRP137692 SRS3127683 GSM3082034 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082034 GSM3082034 GEO Accession GSM3082034 SRX3889830 GSM3082035 SRP137692 SRS3127682 GSM3082035 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082035 GSM3082035 GEO Accession GSM3082035 SRX3889831 GSM3082036 SRP137692 SRS3127684 GSM3082036 ChIP-Seq GENOMIC ChIP Total RNA was extracted with TRIzol (Thermo Fisher) and was cleaned with RNeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. DNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol (Thermo Fisher). ChIP-seq libraries were constructed using Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. RNA-seq library was prepared using Next Ultra Directional RNA Library Prep Kit Illumina (New England Biolabs). ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 6-9 total cycles. Illumina HiSeq 2500 gds 303082036 GSM3082036 GEO Accession GSM3082036 SRX6357997 GSM3903793 SRP137692 PRJNA448883 SRS5020663 GSM3903793 ATAC-seq GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. Illumina HiSeq 2000 gds 303903793 GSM3903793 GEO Accession GSM3903793 SRX6357998 GSM3903794 SRP137692 PRJNA448883 SRS5020664 GSM3903794 ATAC-seq GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. NextSeq 500 gds 303903794 GSM3903794 SRX6357999 GSM3903795 SRP137692 PRJNA448883 SRS5020665 GSM3903795 OTHER GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. NextSeq 500 gds 303903795 GSM3903795 SRX6358000 GSM3903796 SRP137692 PRJNA448883 SRS5020666 GSM3903796 OTHER GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. NextSeq 500 gds 303903796 GSM3903796 SRX6358001 GSM3903797 SRP137692 PRJNA448883 SRS5020667 GSM3903797 OTHER GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. NextSeq 500 gds 303903797 GSM3903797 SRX6358002 GSM3903798 SRP137692 PRJNA448883 SRS5020668 GSM3903798 OTHER GENOMIC other HiChIP was performed as previously described (Mumbach et al 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/ul MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/ul T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). ATAC-seq was performed as described (Buenrostro et al., 2013) on FACS isolated EryP and EryD cells. 2.5 x 104 cells were used per sample. 2.5 l Tn5 Transposases (Illuminar) was used to make the transposition reaction. Libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina HiSeq2000 with 100 bp paired-end reads. HiChIP was performed as previously described (Mumbach et al., 2016) using the Gata1 antibody (ab11852, Abcam). In brief, 15 million of either peripheral blood cells of E10.5 mouse embryos or single cells of E14.5 fetal liver were cross-linked with 1% v/v formaldehyde at room temperature for 10 minutes, and were quenched with 0.125 M Glycine for 5minutes. Crosslinked cells were lysed in cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP40, 1x Roche protease inhibitors) and followed by 1 U/l MboI (NEB) digestion at 37oC for 6 h, fill-in of overhangs with biotin-dATP (Thermo Fisher) at 37oC for 1.5 h, and ligation with 4 U/l T4 DNA Ligase (NEB) at room temperature for 6 h. The nuclear pellet was sheared using a Covaris E220 with following parameters: Duty Factor = 5.0, Peak Power = 140, Cycle/Burst = 200, Time = 240 seconds. Chromatin extracts were immunoprecipitated with Gata1 antibodies overnight at 4oC, and then incubated with protein A Dynabeads (Invitrogen) for another 4 hours. ChIP complexes were eluted and cross-linking reversed by heating at 65oC overnight. DNA was extracted with DNA Clean and Concentrator columns (Zymo Research). For library preparation, Biotin labeled post-ChIP DNA was captured by 5 l Streptavidin C-1 beads (Thermo Fisher), and followed by Tn5 treatment. The amount of Tn5 used and the number of PCR cycles performed was based on the post-ChIP Qubit amounts, as previously described (Mumbach et al., 2016). HiChIP libraries were size-selected for 300-800bp fragments. All libraries were sequenced on the Illumina Nextseq 500 to an average depth of 200 million total reads. NextSeq 500 gds 303903798 GSM3903798