SRX3906783 16p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 272 bp sequencing. SRS3142866 SAMN08874492 16p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906784 18p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 273 bp sequencing. SRS3142867 SAMN08874493 18p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906785 12p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 270 bp sequencing. SRS3142868 SAMN08874490 12p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906786 13p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 271 bp sequencing. SRS3142869 SAMN08874491 13p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906787 22p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 276 bp sequencing. SRS3142870 SAMN08874496 22p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906788 24p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 277 bp sequencing. SRS3142871 SAMN08874497 24p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906789 19p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 274 bp sequencing. SRS3142872 SAMN08874494 19p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906790 1p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 275 bp sequencing. SRS3142873 SAMN08874495 1p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906791 25p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 278 bp sequencing. SRS3142874 SAMN08874498 25p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906792 29p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 279 bp sequencing. SRS3142875 SAMN08874499 29p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906793 11p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 269 bp sequencing. SRS3142876 SAMN08874489 11p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906794 4h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 268 bp sequencing. SRS3142877 SAMN08874488 4h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906795 47h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 267 bp sequencing. SRS3142878 SAMN08874487 47h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906796 45h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 266 bp sequencing. SRS3142879 SAMN08874486 45h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906797 44h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 265 bp sequencing. SRS3142880 SAMN08874485 44h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906798 43h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 264 bp sequencing. SRS3142881 SAMN08874484 43h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906799 41h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 263 bp sequencing. SRS3142882 SAMN08874483 41h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906800 40h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 262 bp sequencing. SRS3142883 SAMN08874482 40h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906801 39h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 261 bp sequencing. SRS3142884 SAMN08874481 39h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906802 37h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 260 bp sequencing. SRS3142885 SAMN08874480 37h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906803 30p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 281 bp sequencing. SRS3142886 SAMN08874501 30p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906804 2p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 280 bp sequencing. SRS3142887 SAMN08874500 2p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906805 34p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 283 bp sequencing. SRS3142888 SAMN08874503 34p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906806 32p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 282 bp sequencing. SRS3142889 SAMN08874502 32p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906807 5p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 285 bp sequencing. SRS3142890 SAMN08874505 5p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906808 3p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 284 bp sequencing. SRS3142891 SAMN08874504 3p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906809 6p_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 286 bp sequencing. SRS3142892 SAMN08874506 6p_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906810 36h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 259 bp sequencing. SRS3142893 SAMN08874479 36h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906811 35h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 258 bp sequencing. SRS3142894 SAMN08874478 35h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906812 21h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 253 bp sequencing. SRS3142895 SAMN08874473 21h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906813 20h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 252 bp sequencing. SRS3142896 SAMN08874472 20h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906814 17h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 251 bp sequencing. SRS3142912 SAMN08874471 17h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906815 15h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 250 bp sequencing. SRS3142897 SAMN08874470 15h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906816 33h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 257 bp sequencing. SRS3142898 SAMN08874477 33h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906817 28h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 256 bp sequencing. SRS3142899 SAMN08874476 28h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906818 27h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 255 bp sequencing. SRS3142900 SAMN08874475 27h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3906819 26h_V2 SRP139003 PRJNA447916 Stool total bacterial DNA extraction was performed using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturer's instructions. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Library concentration and exact product size were measured using a KAPA Library Quantification Kit (Kapa Biosystems,Woburn, MA, USA) and an Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA), respectively. A pooled library (20 nM) and a PhiX control v3 (20 nM) (Illumina) were mixed with 0.2 N fresh NaOH and hybridization buffer HT1 (Illumina) to produce the final concentration at 12 pM each. The resulting library was mixed with the PhiX control v3 (5%, v/v) (Illumina) and 600 L loaded on a MiSeq v2 (500 cycle) Reagent cartridge for obtaining a paired-end 2 254 bp sequencing. SRS3142901 SAMN08874474 26h_V2 AMPLICON METAGENOMIC PCR Illumina MiSeq