SRX3941764 MEM-006-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172374 MEM-006-LOD MEM-006-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2500 SRX3941765 MEM-005-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172373 MEM-005-LOD MEM-005-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2500 SRX3941766 MEM-008-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172372 MEM-008-LOD MEM-008-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2500 SRX3941767 MEM-007-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172376 MEM-007-LOD MEM-007-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2500 SRX3941768 MEM-002-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172375 MEM-002-LOD MEM-002-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2000 SRX3941769 MEM-001-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172377 MEM-001-LOD MEM-001-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2000 SRX3941770 MEM-004-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172379 MEM-004-LOD MEM-004-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2000 SRX3941771 MEM-003-LOD SRP140497 bp0 Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 60 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 180 fragments. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters. Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 4 cycles using 0.5 uM Illuminal universal and sample specific index primers. SRS3172380 MEM-003-LOD MEM-003-LOD Targeted-Capture GENOMIC Hybrid Selection Illumina HiSeq 2000