SRX4232634 Cte-07 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431274 Cte-07 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232635 Cte-05 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431275 Cte-05 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232636 Cte-08 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431273 Cte-08 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232637 Cte-14 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431280 Cte-14 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232638 Cte-12 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431277 Cte-12 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232639 Cte-11 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431282 Cte-11 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232640 Cte-04 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431278 Cte-04 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232641 Cte-03 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431276 Cte-03 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232642 Cte-02 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431279 Cte-02 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232643 Cte-01 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431281 Cte-01 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232644 Cte-06 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431283 Cte-06 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232645 Cte-10 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431284 Cte-10 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000 SRX4232646 Cte-09 SRP150789 A mutagenized library of template DNA was made by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). Mutations were introduced to the aptamer and expression platform domains using a 16 cycle error-prone PCR reaction. Nonmutagenized regions upstream of the aptamer, including a promoter, were added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). This library was used as DNA template for an in vitro transcription reaction containing the specified concentration of ligand (glycine or alanine). DNA template was removed by DNase treatment with TURBO DNase (Thermo Fisher Scientific). A pre-adenylated adaptor was then added to the 3' end using T4 RNA Ligase II, Trunc. K227Q (NEB). Using this adaptor as a handle, the RNA was reverse-transcribed into DNA with SuperScript III (Thermo Fisher Scientific). Illumina adaptors were then added through a two-step PCR reaction with Phusion HF polymerase (NEB or Thermo Fisher Scientific). SRS3431287 Cte-09 RNA-Seq OTHER RT-PCR 300 0 Application Read Forward 1 1 Application Read Reverse 151 Illumina HiSeq 4000