SRX4286574 SF7B6 SRP151167 PRJNA316661 DNA was extracted by using a CTAB lysozyme procedure20. Genomes were sequenced with the Pacific Biosciences RSII (PacBio, USA) single molecule real time (SMRT) system and non-hybrid hierarchical genome (HGAP version 2.3) pre-assembly Quiver (http://www.pacificbiosciences.com/devnet/). Whole genome alignment used the Web ACT program (Artemis Comparison Tool, Center for Bioinformatics). Circular genome mapping and analysis were conducted with BLAST Ring Image Generator (BRIG) v 0.95 (http://sourceforge.net/projects/brig/) SRS3450592 SAMN04589202 SF7B6 WGS GENOMIC size fractionation PacBio RS II