SRX4287437 FW_64 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451250 SAMN09475712 FW_64 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287438 FW_63 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451251 SAMN09475711 FW_63 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287439 FW_62 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451249 SAMN09475710 FW_62 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287440 FW_61 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451252 SAMN09475709 FW_61 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287441 FW_68 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451254 SAMN09475716 FW_68 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287442 FW_67 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451253 SAMN09475715 FW_67 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287443 FW_66 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451255 SAMN09475714 FW_66 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287444 FW_65 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451257 SAMN09475713 FW_65 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287445 FW_70 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451256 SAMN09475718 FW_70 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287446 FW_69 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451258 SAMN09475717 FW_69 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287447 FW_82 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451259 SAMN09475730 FW_82 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287448 FW_81 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451260 SAMN09475729 FW_81 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287449 FW_84 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451261 SAMN09475732 FW_84 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287450 FW_83 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451262 SAMN09475731 FW_83 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287451 FW_86 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451263 SAMN09475734 FW_86 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287452 FW_85 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451264 SAMN09475733 FW_85 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287453 FW_88 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451265 SAMN09475736 FW_88 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287454 FW_87 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451266 SAMN09475735 FW_87 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287455 FW_90 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451267 SAMN09475738 FW_90 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287456 FW_89 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451336 SAMN09475737 FW_89 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287457 FW_79 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451268 SAMN09475727 FW_79 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287458 FW_80 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451269 SAMN09475728 FW_80 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287459 FW_75 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451270 SAMN09475723 FW_75 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287460 FW_76 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451271 SAMN09475724 FW_76 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287461 FW_77 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451272 SAMN09475725 FW_77 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287462 FW_78 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451273 SAMN09475726 FW_78 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287463 FW_71 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451274 SAMN09475719 FW_71 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287464 FW_72 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451275 SAMN09475720 FW_72 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287465 FW_73 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451276 SAMN09475721 FW_73 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287466 FW_74 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451277 SAMN09475722 FW_74 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287467 FW_19 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451278 SAMN09475667 FW_19 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287468 FW_20 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451279 SAMN09475668 FW_20 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287469 FW_13 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451280 SAMN09475661 FW_13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287470 FW_14 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451281 SAMN09475662 FW_14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287471 FW_11 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451283 SAMN09475659 FW_11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287472 FW_12 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451282 SAMN09475660 FW_12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287473 FW_17 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451285 SAMN09475665 FW_17 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287474 FW_18 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451284 SAMN09475666 FW_18 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287475 FW_15 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451286 SAMN09475663 FW_15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287476 FW_16 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451287 SAMN09475664 FW_16 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287477 FW_28 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451289 SAMN09475676 FW_28 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287478 FW_27 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451288 SAMN09475675 FW_27 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287479 FW_26 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451290 SAMN09475674 FW_26 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287480 FW_25 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451291 SAMN09475673 FW_25 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287481 FW_24 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451292 SAMN09475672 FW_24 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287482 FW_23 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451293 SAMN09475671 FW_23 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287483 FW_22 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451294 SAMN09475670 FW_22 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287484 FW_21 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451295 SAMN09475669 FW_21 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287485 FW_30 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451296 SAMN09475678 FW_30 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287486 FW_29 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451297 SAMN09475677 FW_29 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287487 FW_46 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451298 SAMN09475694 FW_46 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287488 FW_45 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451299 SAMN09475693 FW_45 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287489 FW_48 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451300 SAMN09475696 FW_48 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287490 FW_47 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451301 SAMN09475695 FW_47 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287491 FW_42 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451302 SAMN09475690 FW_42 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287492 FW_41 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451303 SAMN09475689 FW_41 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287493 FW_44 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451304 SAMN09475692 FW_44 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287494 FW_43 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451305 SAMN09475691 FW_43 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287495 FW_50 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451306 SAMN09475698 FW_50 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287496 FW_49 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451307 SAMN09475697 FW_49 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287497 FW_31 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451308 SAMN09475679 FW_31 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287498 FW_32 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451310 SAMN09475680 FW_32 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287499 FW_33 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451309 SAMN09475681 FW_33 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287500 FW_34 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451311 SAMN09475682 FW_34 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287501 FW_35 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451312 SAMN09475683 FW_35 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287502 FW_36 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451313 SAMN09475684 FW_36 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287503 FW_37 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451314 SAMN09475685 FW_37 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287504 FW_38 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451315 SAMN09475686 FW_38 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287505 FW_39 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451316 SAMN09475687 FW_39 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287506 FW_40 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451317 SAMN09475688 FW_40 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287507 FW_57 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451318 SAMN09475705 FW_57 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287508 FW_58 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451319 SAMN09475706 FW_58 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287509 FW_55 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451321 SAMN09475703 FW_55 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287510 FW_56 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451320 SAMN09475704 FW_56 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287511 FW_53 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451322 SAMN09475701 FW_53 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287512 FW_54 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451323 SAMN09475702 FW_54 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287513 FW_51 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451324 SAMN09475699 FW_51 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287514 FW_52 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451325 SAMN09475700 FW_52 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287515 FW_59 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451326 SAMN09475707 FW_59 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287516 FW_60 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451327 SAMN09475708 FW_60 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287517 FW_1 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451328 SAMN09475649 FW_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287518 FW_2 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451329 SAMN09475650 FW_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287519 FW_3 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451330 SAMN09475651 FW_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287520 FW_4 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451331 SAMN09475652 FW_4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287521 FW_5 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451332 SAMN09475653 FW_5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287522 FW_6 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451333 SAMN09475654 FW_6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287523 FW_7 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451334 SAMN09475655 FW_7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287524 FW_8 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451335 SAMN09475656 FW_8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287525 FW_9 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451337 SAMN09475657 FW_9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4287526 FW_10 SRP151193 PRJNA477646 The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors. SRS3451338 SAMN09475658 FW_10 AMPLICON METAGENOMIC PCR Illumina MiSeq