SRX4299302 pooled dengue prototype SRP151336 bp0 RNA of five prototype strains were separately extracted and converted to cDNA using serotype specific primer. cDNA of each strain were amplified to two overlapping fragment and mixed together. DNA fragments of five dengue strains were pooled and sequenced in a single sequencing run by 454 GS FLX. SRS3462146 pooled dengue prototype pooled dengue prototype WGS VIRAL RNA RANDOM 454 GS FLX