SRX4320059 GSM3230545 SRP151565 SRS3482682 GSM3230545 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230545 GSM3230545 GEO Accession GSM3230545 SRX4320060 GSM3230546 SRP151565 SRS3482683 GSM3230546 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230546 GSM3230546 GEO Accession GSM3230546 SRX4320061 GSM3230547 SRP151565 SRS3482684 GSM3230547 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230547 GSM3230547 GEO Accession GSM3230547 SRX4320062 GSM3230548 SRP151565 SRS3482725 GSM3230548 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230548 GSM3230548 GEO Accession GSM3230548 SRX4320063 GSM3230549 SRP151565 SRS3482688 GSM3230549 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230549 GSM3230549 GEO Accession GSM3230549 SRX4320064 GSM3230550 SRP151565 SRS3482689 GSM3230550 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230550 GSM3230550 GEO Accession GSM3230550 SRX4320065 GSM3230551 SRP151565 SRS3482686 GSM3230551 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230551 GSM3230551 GEO Accession GSM3230551 SRX4320066 GSM3230552 SRP151565 SRS3482687 GSM3230552 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230552 GSM3230552 GEO Accession GSM3230552 SRX4320067 GSM3230553 SRP151565 SRS3482685 GSM3230553 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230553 GSM3230553 GEO Accession GSM3230553 SRX4320068 GSM3230554 SRP151565 SRS3482690 GSM3230554 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230554 GSM3230554 GEO Accession GSM3230554 SRX4320069 GSM3230555 SRP151565 SRS3482691 GSM3230555 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230555 GSM3230555 GEO Accession GSM3230555 SRX4320070 GSM3230556 SRP151565 SRS3482694 GSM3230556 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230556 GSM3230556 GEO Accession GSM3230556 SRX4320071 GSM3230557 SRP151565 SRS3482693 GSM3230557 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230557 GSM3230557 GEO Accession GSM3230557 SRX4320072 GSM3230558 SRP151565 SRS3482692 GSM3230558 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230558 GSM3230558 GEO Accession GSM3230558 SRX4320073 GSM3230559 SRP151565 SRS3482695 GSM3230559 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230559 GSM3230559 GEO Accession GSM3230559 SRX4320074 GSM3230560 SRP151565 SRS3482696 GSM3230560 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230560 GSM3230560 GEO Accession GSM3230560 SRX4320075 GSM3230561 SRP151565 SRS3482697 GSM3230561 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230561 GSM3230561 GEO Accession GSM3230561 SRX4320076 GSM3230562 SRP151565 SRS3482698 GSM3230562 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230562 GSM3230562 GEO Accession GSM3230562 SRX4320077 GSM3230563 SRP151565 SRS3482699 GSM3230563 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230563 GSM3230563 GEO Accession GSM3230563 SRX4320078 GSM3230564 SRP151565 SRS3482700 GSM3230564 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230564 GSM3230564 GEO Accession GSM3230564 SRX4320079 GSM3230565 SRP151565 SRS3482701 GSM3230565 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230565 GSM3230565 GEO Accession GSM3230565 SRX4320080 GSM3230566 SRP151565 SRS3482704 GSM3230566 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230566 GSM3230566 GEO Accession GSM3230566 SRX4320081 GSM3230567 SRP151565 SRS3482703 GSM3230567 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230567 GSM3230567 GEO Accession GSM3230567 SRX4320082 GSM3230568 SRP151565 SRS3482705 GSM3230568 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230568 GSM3230568 GEO Accession GSM3230568 SRX4320083 GSM3230569 SRP151565 SRS3482702 GSM3230569 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230569 GSM3230569 GEO Accession GSM3230569 SRX4320084 GSM3230570 SRP151565 SRS3482706 GSM3230570 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230570 GSM3230570 GEO Accession GSM3230570 SRX4320085 GSM3230571 SRP151565 SRS3482707 GSM3230571 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230571 GSM3230571 GEO Accession GSM3230571 SRX4320086 GSM3230572 SRP151565 SRS3482708 GSM3230572 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230572 GSM3230572 GEO Accession GSM3230572 SRX4320087 GSM3230573 SRP151565 SRS3482710 GSM3230573 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230573 GSM3230573 GEO Accession GSM3230573 SRX4320088 GSM3230574 SRP151565 SRS3482709 GSM3230574 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230574 GSM3230574 GEO Accession GSM3230574 SRX4320089 GSM3230575 SRP151565 SRS3482713 GSM3230575 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230575 GSM3230575 GEO Accession GSM3230575 SRX4320090 GSM3230576 SRP151565 SRS3482712 GSM3230576 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230576 GSM3230576 GEO Accession GSM3230576 SRX4320091 GSM3230577 SRP151565 SRS3482715 GSM3230577 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230577 GSM3230577 GEO Accession GSM3230577 SRX4320092 GSM3230578 SRP151565 SRS3482714 GSM3230578 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230578 GSM3230578 GEO Accession GSM3230578 SRX4320093 GSM3230579 SRP151565 SRS3482716 GSM3230579 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230579 GSM3230579 GEO Accession GSM3230579 SRX4320094 GSM3230580 SRP151565 SRS3482717 GSM3230580 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230580 GSM3230580 GEO Accession GSM3230580 SRX4320095 GSM3230581 SRP151565 SRS3482718 GSM3230581 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230581 GSM3230581 GEO Accession GSM3230581 SRX4320096 GSM3230582 SRP151565 SRS3482719 GSM3230582 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230582 GSM3230582 GEO Accession GSM3230582 SRX4320097 GSM3230583 SRP151565 SRS3482720 GSM3230583 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230583 GSM3230583 GEO Accession GSM3230583 SRX4320098 GSM3230584 SRP151565 SRS3482721 GSM3230584 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230584 GSM3230584 GEO Accession GSM3230584 SRX4320099 GSM3230585 SRP151565 SRS3482722 GSM3230585 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230585 GSM3230585 GEO Accession GSM3230585 SRX4320100 GSM3230586 SRP151565 SRS3482723 GSM3230586 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230586 GSM3230586 GEO Accession GSM3230586 SRX4320101 GSM3230587 SRP151565 SRS3482724 GSM3230587 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230587 GSM3230587 GEO Accession GSM3230587 SRX4320102 GSM3230588 SRP151565 SRS3482727 GSM3230588 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230588 GSM3230588 GEO Accession GSM3230588 SRX4320103 GSM3230589 SRP151565 SRS3482726 GSM3230589 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230589 GSM3230589 GEO Accession GSM3230589 SRX4320104 GSM3230590 SRP151565 SRS3482728 GSM3230590 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230590 GSM3230590 GEO Accession GSM3230590 SRX4320105 GSM3230591 SRP151565 SRS3482729 GSM3230591 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230591 GSM3230591 GEO Accession GSM3230591 SRX4320106 GSM3230592 SRP151565 SRS3482730 GSM3230592 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from embryos using the MagMAX™-96 Total RNA Isolation kit (ThermoFisher, Waltham, MA) following the manufacturer's protocol supplied with the kit, except as noted below. Homogenization of embryos was performed in 1.5 ml microcentrifuge tubes using a Bullet Blender© Storm 24 homogenizer (Next Advance, Troy, NY) with a 3.2 mm stainless steel bead added to each sample. Each embryo was homogenized in 100 µl of MagMax Lysis/Binding solution supplemented with 0.7 µl β-mercaptoethanol. Contaminating DNA was removed from samples using the supplied Turbo DNase. Both the duration of DNase treatment and incubation temperature were increased, to 20 min and 37 °C, to increase the effectiveness of digestion. RNA was eluted from the magnetic beads using 25 µl of Elution Buffer. During the elution step, samples were incubated at 37 °C Libraries were prepared (n = 16 per treatment) using the TruSeq Stranded mRNA Library Prep Kit for Neoprep (Illumina, San Diego, CA) according to the manufacturer's protocol. One hundred ng RNA was used as input into the automated NeoPrep System (Illumina, San Diego, CA). The NeoPrep is able to prepare libraries for up to 16 samples. Samples were blocked by treatment for a multiplex level of 16 samples. This level of multiplexing provides a theoretical sequencing depth of 18.75 M sequences per sample. Following library preparation, individual samples were quantified using the Qubit fluorometer (Thermofisher), normalized to 10 nM and pooled Illumina HiSeq 4000 gds 303230592 GSM3230592 GEO Accession GSM3230592