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    <TITLE>GSM3239802: MGAS6180 (1); Streptococcus pyogenes; RNA-Seq</TITLE>
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        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was isolated and rRNAs were depleted using the Ribo-Zero Gram-Positive rRNA Removal Kit (Epicentre). The rRNA-depleted RNA was used to generate cDNA libraries using the ScriptSeq kit (Epicentre). Briefly, RNA was fragmented, cDNA was synthesized using random hexamers containing a 5′ tagging sequence, the RNA was hydrolyzed, and the cDNA was tagged at the 3′ end. A limited number of PCR cycles (n=12) were used to amplify the libraries via the 5′ and 3′ tags (the libraries were barcoded by using different primers), and the libraries size-selected (170 to 300 bp). The size-selected and barcoded libraries were run on an Illumina HiSeq flowcell.</LIBRARY_CONSTRUCTION_PROTOCOL>
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    <TITLE>GSM3239803: MGAS6180 (2); Streptococcus pyogenes; RNA-Seq</TITLE>
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    <TITLE>GSM3239804: M28ΔRD2 (1); Streptococcus pyogenes; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP151710</PRIMARY_ID>
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          <SINGLE/>
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        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was isolated and rRNAs were depleted using the Ribo-Zero Gram-Positive rRNA Removal Kit (Epicentre). The rRNA-depleted RNA was used to generate cDNA libraries using the ScriptSeq kit (Epicentre). Briefly, RNA was fragmented, cDNA was synthesized using random hexamers containing a 5′ tagging sequence, the RNA was hydrolyzed, and the cDNA was tagged at the 3′ end. A limited number of PCR cycles (n=12) were used to amplify the libraries via the 5′ and 3′ tags (the libraries were barcoded by using different primers), and the libraries size-selected (170 to 300 bp). The size-selected and barcoded libraries were run on an Illumina HiSeq flowcell.</LIBRARY_CONSTRUCTION_PROTOCOL>
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      <PRIMARY_ID>SRX4328985</PRIMARY_ID>
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    <TITLE>GSM3239805: M28ΔRD2 (2); Streptococcus pyogenes; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP151710</PRIMARY_ID>
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          <SINGLE/>
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        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was isolated and rRNAs were depleted using the Ribo-Zero Gram-Positive rRNA Removal Kit (Epicentre). The rRNA-depleted RNA was used to generate cDNA libraries using the ScriptSeq kit (Epicentre). Briefly, RNA was fragmented, cDNA was synthesized using random hexamers containing a 5′ tagging sequence, the RNA was hydrolyzed, and the cDNA was tagged at the 3′ end. A limited number of PCR cycles (n=12) were used to amplify the libraries via the 5′ and 3′ tags (the libraries were barcoded by using different primers), and the libraries size-selected (170 to 300 bp). The size-selected and barcoded libraries were run on an Illumina HiSeq flowcell.</LIBRARY_CONSTRUCTION_PROTOCOL>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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