SRX4388751 GSM3270821 SRP153371 SRS3545093 GSM3270821 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270821 GSM3270821 GEO Accession GSM3270821 SRX4388752 GSM3270822 SRP153371 SRS3545092 GSM3270822 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270822 GSM3270822 GEO Accession GSM3270822 SRX4388753 GSM3270823 SRP153371 SRS3545094 GSM3270823 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270823 GSM3270823 GEO Accession GSM3270823 SRX4388754 GSM3270824 SRP153371 SRS3545096 GSM3270824 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270824 GSM3270824 GEO Accession GSM3270824 SRX4388755 GSM3270825 SRP153371 SRS3545095 GSM3270825 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270825 GSM3270825 GEO Accession GSM3270825 SRX4388756 GSM3270826 SRP153371 SRS3545099 GSM3270826 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270826 GSM3270826 GEO Accession GSM3270826 SRX4388757 GSM3270827 SRP153371 SRS3545098 GSM3270827 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270827 GSM3270827 GEO Accession GSM3270827 SRX4388758 GSM3270828 SRP153371 SRS3545097 GSM3270828 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270828 GSM3270828 GEO Accession GSM3270828 SRX4388759 GSM3270829 SRP153371 SRS3545100 GSM3270829 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270829 GSM3270829 GEO Accession GSM3270829 SRX4388760 GSM3270830 SRP153371 SRS3545101 GSM3270830 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270830 GSM3270830 GEO Accession GSM3270830 SRX4388761 GSM3270831 SRP153371 SRS3545114 GSM3270831 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270831 GSM3270831 GEO Accession GSM3270831 SRX4388762 GSM3270832 SRP153371 SRS3545103 GSM3270832 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270832 GSM3270832 GEO Accession GSM3270832 SRX4388763 GSM3270833 SRP153371 SRS3545102 GSM3270833 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270833 GSM3270833 GEO Accession GSM3270833 SRX4388764 GSM3270834 SRP153371 SRS3545116 GSM3270834 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270834 GSM3270834 GEO Accession GSM3270834 SRX4388765 GSM3270835 SRP153371 SRS3545104 GSM3270835 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270835 GSM3270835 GEO Accession GSM3270835 SRX4388766 GSM3270836 SRP153371 SRS3545105 GSM3270836 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270836 GSM3270836 GEO Accession GSM3270836 SRX4388767 GSM3270837 SRP153371 SRS3545106 GSM3270837 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270837 GSM3270837 GEO Accession GSM3270837 SRX4388768 GSM3270838 SRP153371 SRS3545107 GSM3270838 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270838 GSM3270838 GEO Accession GSM3270838 SRX4388769 GSM3270839 SRP153371 SRS3545108 GSM3270839 OTHER TRANSCRIPTOMIC other mice were sacrificed by cervical dislocation, whole-body perfusion with PBS was conducted, and organs were extracted for ex vivo analyses. Lymph nodes cells were obtained after grinding the tissue against a 30um filter mesh in Hank's balanced salt solution. A cardiac cell suspension was obtained after enzimatic digestion with Collagenase-II. CD4+ TCRbeta+ single cells were FACSed (FACS Aria-II, purity > 98%) into RLT lysis buffer (Qiagen, Hilden, Germany) and stored at -80oC. RNA purification was performed using the Qiagen microKit plus. RNA quality was checked using the Agilent 2100 Bioanalyzer. TCRb-sequencing was outsourced to the specialized company iRepertoire. For the construction of mouse TCR beta chain libraries, iRepertoire used amplicon rescued multiplex PCR (arm-PCR) to amplify each of the 19 submitted RNA samples. The cDNA synthesis protocol involved initial first-round RT-PCR using high concentrations of gene-specific primers (iRepertoire's MTBIvc primers), followed by Beckman Coulter's SPRIselect bead-cleanup procedure, and PCR2 using universal primers to amplify the cDNA exponentially. Each constructed library was measured for concentration and quality using Thermoscientific's Nanodrop 1000, then pooled using 200 ng per constructed library. This pool was QC'ed using a bioanalyzer, KAPA qPCR analysis, and Qubit quantitation. The QC'ed pool was then sequenced according to Illumina's HiSeq sequencing guidelines using a 300-cycle reagent kit. Libraries were pooled and sequenced with the Illumina HiSeq. The output of the mouse TRB sequence covers within the third framework region through the CDR3 and the beginning of the constant region. Illumina HiSeq 1000 gds 303270839 GSM3270839 GEO Accession GSM3270839