SRX4535286 831_E9_hg38_chr17:74968459-74968481_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653157 831_E9_hg38_chr17:74968459-74968481_HEK293_edited_rep_1 831_E9_hg38_chr17:74968459-74968481_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535287 1734_E3_hg38_chr8:143666899-143666921_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653159 1734_E3_hg38_chr8:143666899-143666921_HEK293_control_rep_1 1734_E3_hg38_chr8:143666899-143666921_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535288 840_A2_hg19_chr5:169511413-169511435_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653160 840_A2_hg19_chr5:169511413-169511435_HEK293_edited_rep_1 840_A2_hg19_chr5:169511413-169511435_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535289 831_D9_hg38_chr17:74892980-74893002_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653158 831_D9_hg38_chr17:74892980-74893002_HEK293_edited_rep_1 831_D9_hg38_chr17:74892980-74893002_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535290 836_C10_hg19_chr5:176741530-176741552_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653162 836_C10_hg19_chr5:176741530-176741552_HEK293_control_rep_1 836_C10_hg19_chr5:176741530-176741552_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535291 836_H8_hg19_chr12:9540554-9540576_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653161 836_H8_hg19_chr12:9540554-9540576_HEK293_control_rep_1 836_H8_hg19_chr12:9540554-9540576_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535292 836_A9_hg19_chr7:151103009-151103031_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653163 836_A9_hg19_chr7:151103009-151103031_HEK293_control_rep_1 836_A9_hg19_chr7:151103009-151103031_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535293 836_F8_hg19_chr17:80776142-80776164_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653164 836_F8_hg19_chr17:80776142-80776164_HEK293_control_rep_1 836_F8_hg19_chr17:80776142-80776164_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535294 836_G8_hg19_chr2:101551413-101551435_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653165 836_G8_hg19_chr2:101551413-101551435_HEK293_control_rep_1 836_G8_hg19_chr2:101551413-101551435_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535295 836_D8_hg19_chr15:96865161-96865183_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653166 836_D8_hg19_chr15:96865161-96865183_HEK293_control_rep_1 836_D8_hg19_chr15:96865161-96865183_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535296 836_E8_hg19_chr2:148314533-148314555_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653169 836_E8_hg19_chr2:148314533-148314555_HEK293_control_rep_1 836_E8_hg19_chr2:148314533-148314555_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535297 836_B8_hg19_chr4:182898567-182898589_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653168 836_B8_hg19_chr4:182898567-182898589_HEK293_control_rep_1 836_B8_hg19_chr4:182898567-182898589_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535298 836_C8_hg19_chr16:84886878-84886900_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653167 836_C8_hg19_chr16:84886878-84886900_HEK293_control_rep_1 836_C8_hg19_chr16:84886878-84886900_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535299 836_H7_hg19_chr6:166184438-166184460_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653171 836_H7_hg19_chr6:166184438-166184460_HEK293_control_rep_1 836_H7_hg19_chr6:166184438-166184460_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535300 836_A8_hg19_chr3:85611236-85611258_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653170 836_A8_hg19_chr3:85611236-85611258_HEK293_control_rep_1 836_A8_hg19_chr3:85611236-85611258_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535301 839_E4_hg19_chr14:103398376-103398398_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653172 839_E4_hg19_chr14:103398376-103398398_HEK293_edited_rep_1 839_E4_hg19_chr14:103398376-103398398_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535302 1734_E4_hg38_chr1:6300948-6300970_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653173 1734_E4_hg38_chr1:6300948-6300970_HEK293_control_rep_1 1734_E4_hg38_chr1:6300948-6300970_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535303 1734_E2_hg38_chr4:184260137-184260159_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653174 1734_E2_hg38_chr4:184260137-184260159_HEK293_control_rep_1 1734_E2_hg38_chr4:184260137-184260159_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535304 831_C10_hg38_chr18:28129593-28129615_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653175 831_C10_hg38_chr18:28129593-28129615_HEK293_edited_rep_1 831_C10_hg38_chr18:28129593-28129615_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535305 1734_D4_hg38_chr2:147556965-147556987_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653176 1734_D4_hg38_chr2:147556965-147556987_HEK293_control_rep_1 1734_D4_hg38_chr2:147556965-147556987_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535306 832_G5_hg38_chr2:119066831-119066853_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653177 832_G5_hg38_chr2:119066831-119066853_HEK293_edited_rep_1 832_G5_hg38_chr2:119066831-119066853_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535307 832_F5_hg38_chr2:118070071-118070093_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653181 832_F5_hg38_chr2:118070071-118070093_HEK293_edited_rep_1 832_F5_hg38_chr2:118070071-118070093_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535308 831_B10_hg38_chr18:366708-366730_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653178 831_B10_hg38_chr18:366708-366730_HEK293_edited_rep_1 831_B10_hg38_chr18:366708-366730_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535309 1734_C4_hg38_chr1:181220941-181220963_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653182 1734_C4_hg38_chr1:181220941-181220963_HEK293_control_rep_1 1734_C4_hg38_chr1:181220941-181220963_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535310 832_G4_hg38_chr2:85810772-85810794_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653180 832_G4_hg38_chr2:85810772-85810794_HEK293_edited_rep_1 832_G4_hg38_chr2:85810772-85810794_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535311 832_F4_hg38_chr2:76397546-76397568_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653183 832_F4_hg38_chr2:76397546-76397568_HEK293_edited_rep_1 832_F4_hg38_chr2:76397546-76397568_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535312 832_A5_hg38_chr2:95390891-95390913_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653184 832_A5_hg38_chr2:95390891-95390913_HEK293_edited_rep_1 832_A5_hg38_chr2:95390891-95390913_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535313 832_H4_hg38_chr2:88127149-88127171_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653179 832_H4_hg38_chr2:88127149-88127171_HEK293_edited_rep_1 832_H4_hg38_chr2:88127149-88127171_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535314 832_C5_hg38_chr2:104399495-104399517_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653187 832_C5_hg38_chr2:104399495-104399517_HEK293_edited_rep_1 832_C5_hg38_chr2:104399495-104399517_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535315 832_B5_hg38_chr2:96702795-96702817_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653186 832_B5_hg38_chr2:96702795-96702817_HEK293_edited_rep_1 832_B5_hg38_chr2:96702795-96702817_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535316 832_E5_hg38_chr2:113232729-113232751_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653185 832_E5_hg38_chr2:113232729-113232751_HEK293_edited_rep_1 832_E5_hg38_chr2:113232729-113232751_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535317 832_D5_hg38_chr2:108321838-108321860_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653188 832_D5_hg38_chr2:108321838-108321860_HEK293_edited_rep_1 832_D5_hg38_chr2:108321838-108321860_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535318 838_C4_hg19_chr9:130268812-130268834_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653192 838_C4_hg19_chr9:130268812-130268834_HEK293_edited_rep_1 838_C4_hg19_chr9:130268812-130268834_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535319 838_B4_hg19_chr5:858465-858487_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653191 838_B4_hg19_chr5:858465-858487_HEK293_edited_rep_1 838_B4_hg19_chr5:858465-858487_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535320 838_A4_hg19_chr19:35022998-35023020_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653189 838_A4_hg19_chr19:35022998-35023020_HEK293_edited_rep_1 838_A4_hg19_chr19:35022998-35023020_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535321 838_H3_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653190 838_H3_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 838_H3_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535322 838_G4_hg19_chr7:30946099-30946121_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653193 838_G4_hg19_chr7:30946099-30946121_HEK293_edited_rep_1 838_G4_hg19_chr7:30946099-30946121_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535323 838_F4_hg19_chrX:11701311-11701333_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653194 838_F4_hg19_chrX:11701311-11701333_HEK293_edited_rep_1 838_F4_hg19_chrX:11701311-11701333_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535324 838_E4_hg19_chr16:31484903-31484925_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653195 838_E4_hg19_chr16:31484903-31484925_HEK293_edited_rep_1 838_E4_hg19_chr16:31484903-31484925_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535325 838_D4_hg19_chr17:43334265-43334287_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653196 838_D4_hg19_chr17:43334265-43334287_HEK293_edited_rep_1 838_D4_hg19_chr17:43334265-43334287_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535326 839_F4_hg19_chr17:80529816-80529838_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653198 839_F4_hg19_chr17:80529816-80529838_HEK293_edited_rep_1 839_F4_hg19_chr17:80529816-80529838_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535327 839_G4_hg19_chr10:133014883-133014905_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653199 839_G4_hg19_chr10:133014883-133014905_HEK293_edited_rep_1 839_G4_hg19_chr10:133014883-133014905_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535328 838_A5_hg19_chr17:73608985-73609007_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653200 838_A5_hg19_chr17:73608985-73609007_HEK293_edited_rep_1 838_A5_hg19_chr17:73608985-73609007_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535329 838_H4_hg19_chr16:68431546-68431568_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653197 838_H4_hg19_chr16:68431546-68431568_HEK293_edited_rep_1 838_H4_hg19_chr16:68431546-68431568_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535330 839_B5_hg19_chr17:34883889-34883911_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653201 839_B5_hg19_chr17:34883889-34883911_HEK293_edited_rep_1 839_B5_hg19_chr17:34883889-34883911_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535331 838_F5_hg19_chr15:89175080-89175102_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653202 838_F5_hg19_chr15:89175080-89175102_HEK293_edited_rep_1 838_F5_hg19_chr15:89175080-89175102_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535332 839_H4_hg19_chr3:48606566-48606588_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653290 839_H4_hg19_chr3:48606566-48606588_HEK293_edited_rep_1 839_H4_hg19_chr3:48606566-48606588_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535333 839_A5_hg19_chr8:23409382-23409404_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653203 839_A5_hg19_chr8:23409382-23409404_HEK293_edited_rep_1 839_A5_hg19_chr8:23409382-23409404_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535334 836_E3_hg19_chr3:148674153-148674175_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653205 836_E3_hg19_chr3:148674153-148674175_HEK293_control_rep_1 836_E3_hg19_chr3:148674153-148674175_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535335 836_D3_hg19_chr6:155472166-155472188_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653206 836_D3_hg19_chr6:155472166-155472188_HEK293_control_rep_1 836_D3_hg19_chr6:155472166-155472188_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535336 836_G3_hg19_chr22:23427175-23427197_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653204 836_G3_hg19_chr22:23427175-23427197_HEK293_control_rep_1 836_G3_hg19_chr22:23427175-23427197_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535337 836_F3_hg19_chr5:176638327-176638349_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653207 836_F3_hg19_chr5:176638327-176638349_HEK293_control_rep_1 836_F3_hg19_chr5:176638327-176638349_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535338 836_A3_hg19_chr3:186559608-186559630_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653208 836_A3_hg19_chr3:186559608-186559630_HEK293_control_rep_1 836_A3_hg19_chr3:186559608-186559630_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535339 836_H2_hg19_chr14:65417229-65417251_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653209 836_H2_hg19_chr14:65417229-65417251_HEK293_control_rep_1 836_H2_hg19_chr14:65417229-65417251_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535340 836_C3_hg19_chr11:117711639-117711661_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653210 836_C3_hg19_chr11:117711639-117711661_HEK293_control_rep_1 836_C3_hg19_chr11:117711639-117711661_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535341 836_B3_hg19_chr15:63199386-63199408_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653214 836_B3_hg19_chr15:63199386-63199408_HEK293_control_rep_1 836_B3_hg19_chr15:63199386-63199408_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535342 838_D5_hg19_chr12:42790960-42790982_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653212 838_D5_hg19_chr12:42790960-42790982_HEK293_edited_rep_1 838_D5_hg19_chr12:42790960-42790982_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535343 836_A4_hg19_chr10:133774386-133774408_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653213 836_A4_hg19_chr10:133774386-133774408_HEK293_control_rep_1 836_A4_hg19_chr10:133774386-133774408_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535344 836_H3_hg19_chr11:46498098-46498120_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653215 836_H3_hg19_chr11:46498098-46498120_HEK293_control_rep_1 836_H3_hg19_chr11:46498098-46498120_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535345 839_C5_hg19_chr1:6529315-6529337_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653211 839_C5_hg19_chr1:6529315-6529337_HEK293_edited_rep_1 839_C5_hg19_chr1:6529315-6529337_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535346 838_E5_hg19_chr8:10472670-10472692_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653216 838_E5_hg19_chr8:10472670-10472692_HEK293_edited_rep_1 838_E5_hg19_chr8:10472670-10472692_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535347 838_G5_hg19_chr12:57006033-57006055_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653217 838_G5_hg19_chr12:57006033-57006055_HEK293_edited_rep_1 838_G5_hg19_chr12:57006033-57006055_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535348 838_G12_hg19_chr4:183758607-183758629_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653219 838_G12_hg19_chr4:183758607-183758629_HEK293_edited_rep_1 838_G12_hg19_chr4:183758607-183758629_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535349 838_B5_hg19_chr6:159181238-159181260_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653221 838_B5_hg19_chr6:159181238-159181260_HEK293_edited_rep_1 838_B5_hg19_chr6:159181238-159181260_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535350 838_C5_hg19_chr10:87923063-87923085_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653222 838_C5_hg19_chr10:87923063-87923085_HEK293_edited_rep_1 838_C5_hg19_chr10:87923063-87923085_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535351 1734_D6_hg38_chr20:20329213-20329235_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653220 1734_D6_hg38_chr20:20329213-20329235_HEK293_control_rep_1 1734_D6_hg38_chr20:20329213-20329235_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535352 1734_E6_hg38_chr12:132672514-132672536_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653300 1734_E6_hg38_chr12:132672514-132672536_HEK293_control_rep_1 1734_E6_hg38_chr12:132672514-132672536_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535353 1734_B4_hg38_chr2:69498921-69498943_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653218 1734_B4_hg38_chr2:69498921-69498943_HEK293_control_rep_1 1734_B4_hg38_chr2:69498921-69498943_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535354 837_H7_hg19_chr1:175846343-175846365_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653223 837_H7_hg19_chr1:175846343-175846365_HEK293_control_rep_1 837_H7_hg19_chr1:175846343-175846365_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535355 837_A8_hg19_chr4:2294117-2294139_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653224 837_A8_hg19_chr4:2294117-2294139_HEK293_control_rep_1 837_A8_hg19_chr4:2294117-2294139_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535356 837_F7_hg19_chr1:41235601-41235623_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653225 837_F7_hg19_chr1:41235601-41235623_HEK293_control_rep_1 837_F7_hg19_chr1:41235601-41235623_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535357 837_G7_hg19_chr1:12625319-12625341_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653226 837_G7_hg19_chr1:12625319-12625341_HEK293_control_rep_1 837_G7_hg19_chr1:12625319-12625341_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535358 837_D7_hg19_chr1:16318642-16318664_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653227 837_D7_hg19_chr1:16318642-16318664_HEK293_control_rep_1 837_D7_hg19_chr1:16318642-16318664_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535359 837_E7_hg19_chr1:21928481-21928503_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653229 837_E7_hg19_chr1:21928481-21928503_HEK293_control_rep_1 837_E7_hg19_chr1:21928481-21928503_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535360 837_B7_hg19_chr11:125589348-125589370_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653228 837_B7_hg19_chr11:125589348-125589370_HEK293_control_rep_1 837_B7_hg19_chr11:125589348-125589370_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535361 837_C7_hg19_chr15:41059404-41059426_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653232 837_C7_hg19_chr15:41059404-41059426_HEK293_control_rep_1 837_C7_hg19_chr15:41059404-41059426_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535362 837_B8_hg19_chr13:20176711-20176733_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653230 837_B8_hg19_chr13:20176711-20176733_HEK293_control_rep_1 837_B8_hg19_chr13:20176711-20176733_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535363 837_C8_hg19_chr14:103273237-103273259_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653231 837_C8_hg19_chr14:103273237-103273259_HEK293_control_rep_1 837_C8_hg19_chr14:103273237-103273259_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535364 837_A12_hg19_chr22:20167209-20167231_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653233 837_A12_hg19_chr22:20167209-20167231_HEK293_control_rep_1 837_A12_hg19_chr22:20167209-20167231_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535365 834_C8_hg38_chr6:166768192-166768214_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653234 834_C8_hg38_chr6:166768192-166768214_HEK293_edited_rep_1 834_C8_hg38_chr6:166768192-166768214_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535366 834_B8_hg38_chr6:164327314-164327336_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653235 834_B8_hg38_chr6:164327314-164327336_HEK293_edited_rep_1 834_B8_hg38_chr6:164327314-164327336_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535367 834_E8_hg38_chr7:3009200-3009222_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653236 834_E8_hg38_chr7:3009200-3009222_HEK293_edited_rep_1 834_E8_hg38_chr7:3009200-3009222_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535368 834_D8_hg38_chr6:167714050-167714072_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653237 834_D8_hg38_chr6:167714050-167714072_HEK293_edited_rep_1 834_D8_hg38_chr6:167714050-167714072_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535369 834_G8_hg38_chr7:4581061-4581083_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653239 834_G8_hg38_chr7:4581061-4581083_HEK293_edited_rep_1 834_G8_hg38_chr7:4581061-4581083_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535370 834_F8_hg38_chr7:4245211-4245233_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653238 834_F8_hg38_chr7:4245211-4245233_HEK293_edited_rep_1 834_F8_hg38_chr7:4245211-4245233_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535371 834_A9_hg38_chr7:26159207-26159229_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653240 834_A9_hg38_chr7:26159207-26159229_HEK293_edited_rep_1 834_A9_hg38_chr7:26159207-26159229_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535372 834_H8_hg38_chr7:21519826-21519848_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653241 834_H8_hg38_chr7:21519826-21519848_HEK293_edited_rep_1 834_H8_hg38_chr7:21519826-21519848_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535373 834_C9_hg38_chr7:27186065-27186087_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653242 834_C9_hg38_chr7:27186065-27186087_HEK293_edited_rep_1 834_C9_hg38_chr7:27186065-27186087_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535374 834_B9_hg38_chr7:26551139-26551161_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653321 834_B9_hg38_chr7:26551139-26551161_HEK293_edited_rep_1 834_B9_hg38_chr7:26551139-26551161_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535375 840_E2_hg19_chr2:45523611-45523633_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653243 840_E2_hg19_chr2:45523611-45523633_HEK293_edited_rep_1 840_E2_hg19_chr2:45523611-45523633_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535376 840_D2_hg19_chr11:132300785-132300807_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653244 840_D2_hg19_chr11:132300785-132300807_HEK293_edited_rep_1 840_D2_hg19_chr11:132300785-132300807_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535377 840_C3_hg19_chr19:40898615-40898637_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653246 840_C3_hg19_chr19:40898615-40898637_HEK293_edited_rep_1 840_C3_hg19_chr19:40898615-40898637_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535378 840_B3_hg19_chr20:31098410-31098432_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653245 840_B3_hg19_chr20:31098410-31098432_HEK293_edited_rep_1 840_B3_hg19_chr20:31098410-31098432_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535379 840_A3_hg19_chr10:128585309-128585331_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653248 840_A3_hg19_chr10:128585309-128585331_HEK293_edited_rep_1 840_A3_hg19_chr10:128585309-128585331_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535380 840_H2_hg19_chr5:54187613-54187635_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653247 840_H2_hg19_chr5:54187613-54187635_HEK293_edited_rep_1 840_H2_hg19_chr5:54187613-54187635_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535381 831_G7_hg38_chr17:44025246-44025268_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653249 831_G7_hg38_chr17:44025246-44025268_HEK293_edited_rep_1 831_G7_hg38_chr17:44025246-44025268_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535382 831_F7_hg38_chr17:42698738-42698760_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653252 831_F7_hg38_chr17:42698738-42698760_HEK293_edited_rep_1 831_F7_hg38_chr17:42698738-42698760_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535383 837_H11_hg19_chr19:55627117-55627139_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653251 837_H11_hg19_chr19:55627117-55627139_HEK293_control_rep_1 837_H11_hg19_chr19:55627117-55627139_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535384 840_A10_hg19_chr2:110014698-110014720_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653250 840_A10_hg19_chr2:110014698-110014720_HEK293_edited_rep_1 840_A10_hg19_chr2:110014698-110014720_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535385 838_E7_hg19_chr16:1153458-1153480_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653253 838_E7_hg19_chr16:1153458-1153480_HEK293_edited_rep_1 838_E7_hg19_chr16:1153458-1153480_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535386 838_D7_hg19_chr2:27039862-27039884_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653254 838_D7_hg19_chr2:27039862-27039884_HEK293_edited_rep_1 838_D7_hg19_chr2:27039862-27039884_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535387 840_D10_hg19_chr15:29414767-29414789_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653255 840_D10_hg19_chr15:29414767-29414789_HEK293_edited_rep_1 840_D10_hg19_chr15:29414767-29414789_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535388 840_E10_hg19_chr1:232797737-232797759_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653257 840_E10_hg19_chr1:232797737-232797759_HEK293_edited_rep_1 840_E10_hg19_chr1:232797737-232797759_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535389 831_G6_hg38_chr17:27857294-27857316_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653256 831_G6_hg38_chr17:27857294-27857316_HEK293_edited_rep_1 831_G6_hg38_chr17:27857294-27857316_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535390 831_F6_hg38_chr17:22521284-22521306_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653258 831_F6_hg38_chr17:22521284-22521306_HEK293_edited_rep_1 831_F6_hg38_chr17:22521284-22521306_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535391 831_A7_hg38_chr17:31388828-31388850_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653259 831_A7_hg38_chr17:31388828-31388850_HEK293_edited_rep_1 831_A7_hg38_chr17:31388828-31388850_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535392 831_H6_hg38_chr17:28684330-28684352_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653261 831_H6_hg38_chr17:28684330-28684352_HEK293_edited_rep_1 831_H6_hg38_chr17:28684330-28684352_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535393 831_C7_hg38_chr17:34659300-34659322_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653260 831_C7_hg38_chr17:34659300-34659322_HEK293_edited_rep_1 831_C7_hg38_chr17:34659300-34659322_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535394 831_B7_hg38_chr17:31425937-31425959_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653262 831_B7_hg38_chr17:31425937-31425959_HEK293_edited_rep_1 831_B7_hg38_chr17:31425937-31425959_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535395 831_E7_hg38_chr17:41913038-41913060_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653263 831_E7_hg38_chr17:41913038-41913060_HEK293_edited_rep_1 831_E7_hg38_chr17:41913038-41913060_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535396 831_D7_hg38_chr17:41640070-41640092_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653264 831_D7_hg38_chr17:41640070-41640092_HEK293_edited_rep_1 831_D7_hg38_chr17:41640070-41640092_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535397 837_B1_hg19_chr3:71744235-71744257_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653265 837_B1_hg19_chr3:71744235-71744257_HEK293_control_rep_1 837_B1_hg19_chr3:71744235-71744257_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535398 837_C1_hg19_chr6:137307894-137307916_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653267 837_C1_hg19_chr6:137307894-137307916_HEK293_control_rep_1 837_C1_hg19_chr6:137307894-137307916_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535399 836_H12_hg19_chr16:32037129-32037151_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653266 836_H12_hg19_chr16:32037129-32037151_HEK293_control_rep_1 836_H12_hg19_chr16:32037129-32037151_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535400 837_A1_hg19_chr16:32980690-32980712_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653268 837_A1_hg19_chr16:32980690-32980712_HEK293_control_rep_1 837_A1_hg19_chr16:32980690-32980712_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535401 833_E12_hg38_chr5:14223412-14223434_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653269 833_E12_hg38_chr5:14223412-14223434_HEK293_edited_rep_1 833_E12_hg38_chr5:14223412-14223434_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535402 833_D12_hg38_chr5:7067029-7067051_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653270 833_D12_hg38_chr5:7067029-7067051_HEK293_edited_rep_1 833_D12_hg38_chr5:7067029-7067051_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535403 837_D1_hg19_chr3:25347412-25347434_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653271 837_D1_hg19_chr3:25347412-25347434_HEK293_control_rep_1 837_D1_hg19_chr3:25347412-25347434_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535404 837_E1_hg19_chr15:66292443-66292465_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653272 837_E1_hg19_chr15:66292443-66292465_HEK293_control_rep_1 837_E1_hg19_chr15:66292443-66292465_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535405 833_A12_hg38_chr4:184325001-184325023_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653273 833_A12_hg38_chr4:184325001-184325023_HEK293_edited_rep_1 833_A12_hg38_chr4:184325001-184325023_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535406 833_H11_hg38_chr4:183455764-183455786_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653274 833_H11_hg38_chr4:183455764-183455786_HEK293_edited_rep_1 833_H11_hg38_chr4:183455764-183455786_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535407 833_C12_hg38_chr5:2823512-2823534_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653275 833_C12_hg38_chr5:2823512-2823534_HEK293_edited_rep_1 833_C12_hg38_chr5:2823512-2823534_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535408 833_B12_hg38_chr5:2536523-2536545_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653276 833_B12_hg38_chr5:2536523-2536545_HEK293_edited_rep_1 833_B12_hg38_chr5:2536523-2536545_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535409 833_E11_hg38_chr4:156496792-156496814_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653358 833_E11_hg38_chr4:156496792-156496814_HEK293_edited_rep_1 833_E11_hg38_chr4:156496792-156496814_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535410 833_D11_hg38_chr4:151709920-151709942_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653277 833_D11_hg38_chr4:151709920-151709942_HEK293_edited_rep_1 833_D11_hg38_chr4:151709920-151709942_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535411 833_G11_hg38_chr4:179017703-179017725_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653359 833_G11_hg38_chr4:179017703-179017725_HEK293_edited_rep_1 833_G11_hg38_chr4:179017703-179017725_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535412 833_F11_hg38_chr4:169303006-169303028_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653278 833_F11_hg38_chr4:169303006-169303028_HEK293_edited_rep_1 833_F11_hg38_chr4:169303006-169303028_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535413 839_F11_hg19_chr6:158387927-158387949_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653279 839_F11_hg19_chr6:158387927-158387949_HEK293_edited_rep_1 839_F11_hg19_chr6:158387927-158387949_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535414 839_G11_hg19_chr2:8357445-8357467_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653280 839_G11_hg19_chr2:8357445-8357467_HEK293_edited_rep_1 839_G11_hg19_chr2:8357445-8357467_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535415 839_D11_hg19_chr6:34439724-34439746_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653281 839_D11_hg19_chr6:34439724-34439746_HEK293_edited_rep_1 839_D11_hg19_chr6:34439724-34439746_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535416 839_E11_hg19_chr22:22874988-22875010_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653282 839_E11_hg19_chr22:22874988-22875010_HEK293_edited_rep_1 839_E11_hg19_chr22:22874988-22875010_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535417 839_B11_hg19_chr2:16151657-16151679_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653283 839_B11_hg19_chr2:16151657-16151679_HEK293_edited_rep_1 839_B11_hg19_chr2:16151657-16151679_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535418 839_C11_hg19_chr17:70285215-70285237_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653284 839_C11_hg19_chr17:70285215-70285237_HEK293_edited_rep_1 839_C11_hg19_chr17:70285215-70285237_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535419 839_H10_hg19_chr22:37508524-37508546_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653285 839_H10_hg19_chr22:37508524-37508546_HEK293_edited_rep_1 839_H10_hg19_chr22:37508524-37508546_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535420 839_A11_hg19_chr16:49848326-49848348_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653286 839_A11_hg19_chr16:49848326-49848348_HEK293_edited_rep_1 839_A11_hg19_chr16:49848326-49848348_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535421 839_F10_hg19_chr3:63898822-63898844_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653287 839_F10_hg19_chr3:63898822-63898844_HEK293_edited_rep_1 839_F10_hg19_chr3:63898822-63898844_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535422 839_G10_hg19_chr2:112173057-112173079_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653288 839_G10_hg19_chr2:112173057-112173079_HEK293_edited_rep_1 839_G10_hg19_chr2:112173057-112173079_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535423 835_A11_hg19_chr11:68201475-68201497_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653289 835_A11_hg19_chr11:68201475-68201497_HEK293_control_rep_1 835_A11_hg19_chr11:68201475-68201497_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535424 835_H10_hg19_chr2:43201029-43201051_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653291 835_H10_hg19_chr2:43201029-43201051_HEK293_control_rep_1 835_H10_hg19_chr2:43201029-43201051_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535425 835_A10_hg19_chr10:131639488-131639510_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653292 835_A10_hg19_chr10:131639488-131639510_HEK293_control_rep_1 835_A10_hg19_chr10:131639488-131639510_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535426 835_H9_hg19_chr4:109424089-109424111_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653294 835_H9_hg19_chr4:109424089-109424111_HEK293_control_rep_1 835_H9_hg19_chr4:109424089-109424111_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535427 835_C10_hg19_chr14:88659440-88659462_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653293 835_C10_hg19_chr14:88659440-88659462_HEK293_control_rep_1 835_C10_hg19_chr14:88659440-88659462_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535428 835_B10_hg19_chr1:32043285-32043307_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653295 835_B10_hg19_chr1:32043285-32043307_HEK293_control_rep_1 835_B10_hg19_chr1:32043285-32043307_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535429 835_E10_hg19_chr12:48218221-48218243_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653298 835_E10_hg19_chr12:48218221-48218243_HEK293_control_rep_1 835_E10_hg19_chr12:48218221-48218243_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535430 835_D10_hg19_chr10:105830316-105830338_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653297 835_D10_hg19_chr10:105830316-105830338_HEK293_control_rep_1 835_D10_hg19_chr10:105830316-105830338_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535431 835_G10_hg19_chr22:46954109-46954131_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653296 835_G10_hg19_chr22:46954109-46954131_HEK293_control_rep_1 835_G10_hg19_chr22:46954109-46954131_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535432 835_F10_hg19_chr1:154801598-154801620_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653299 835_F10_hg19_chr1:154801598-154801620_HEK293_control_rep_1 835_F10_hg19_chr1:154801598-154801620_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535433 840_D11_hg19_chr5:61100911-61100933_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653303 840_D11_hg19_chr5:61100911-61100933_HEK293_edited_rep_1 840_D11_hg19_chr5:61100911-61100933_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535434 836_C9_hg19_chr8:23351234-23351256_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653302 836_C9_hg19_chr8:23351234-23351256_HEK293_control_rep_1 836_C9_hg19_chr8:23351234-23351256_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535435 836_B9_hg19_chr2:27603837-27603859_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653301 836_B9_hg19_chr2:27603837-27603859_HEK293_control_rep_1 836_B9_hg19_chr2:27603837-27603859_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535436 838_B1_hg19_chr16:10133368-10133390_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653304 838_B1_hg19_chr16:10133368-10133390_HEK293_edited_rep_1 838_B1_hg19_chr16:10133368-10133390_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535437 838_C1_hg19_chr11:62101680-62101702_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653305 838_C1_hg19_chr11:62101680-62101702_HEK293_edited_rep_1 838_C1_hg19_chr11:62101680-62101702_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535438 1734_D5_hg38_chr1:40769929-40769951_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653308 1734_D5_hg38_chr1:40769929-40769951_HEK293_control_rep_1 1734_D5_hg38_chr1:40769929-40769951_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535439 833_D1_hg38_chr22:24160640-24160662_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653306 833_D1_hg38_chr22:24160640-24160662_HEK293_edited_rep_1 833_D1_hg38_chr22:24160640-24160662_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535440 833_E1_hg38_chr22:25982750-25982772_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653307 833_E1_hg38_chr22:25982750-25982772_HEK293_edited_rep_1 833_E1_hg38_chr22:25982750-25982772_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535441 833_F1_hg38_chr22:26373859-26373881_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653309 833_F1_hg38_chr22:26373859-26373881_HEK293_edited_rep_1 833_F1_hg38_chr22:26373859-26373881_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535442 833_G1_hg38_chr22:26544483-26544505_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653310 833_G1_hg38_chr22:26544483-26544505_HEK293_edited_rep_1 833_G1_hg38_chr22:26544483-26544505_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535443 833_H1_hg38_chr22:28107968-28107990_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653313 833_H1_hg38_chr22:28107968-28107990_HEK293_edited_rep_1 833_H1_hg38_chr22:28107968-28107990_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535444 833_A2_hg38_chr22:28802492-28802514_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653311 833_A2_hg38_chr22:28802492-28802514_HEK293_edited_rep_1 833_A2_hg38_chr22:28802492-28802514_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535445 833_B2_hg38_chr22:29697393-29697415_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653314 833_B2_hg38_chr22:29697393-29697415_HEK293_edited_rep_1 833_B2_hg38_chr22:29697393-29697415_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535446 833_C2_hg38_chr22:35681958-35681980_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653312 833_C2_hg38_chr22:35681958-35681980_HEK293_edited_rep_1 833_C2_hg38_chr22:35681958-35681980_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535447 833_D2_hg38_chr22:37642234-37642256_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653315 833_D2_hg38_chr22:37642234-37642256_HEK293_edited_rep_1 833_D2_hg38_chr22:37642234-37642256_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535448 833_E2_hg38_chr22:38755230-38755252_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653317 833_E2_hg38_chr22:38755230-38755252_HEK293_edited_rep_1 833_E2_hg38_chr22:38755230-38755252_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535449 837_B12_hg19_chr1:942044-942066_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653476 837_B12_hg19_chr1:942044-942066_HEK293_control_rep_1 837_B12_hg19_chr1:942044-942066_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535450 836_B10_hg19_chr11:11302409-11302431_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653316 836_B10_hg19_chr11:11302409-11302431_HEK293_control_rep_1 836_B10_hg19_chr11:11302409-11302431_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535451 837_H12_hg19_chr3:128295785-128295807_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653318 837_H12_hg19_chr3:128295785-128295807_HEK293_control_rep_1 837_H12_hg19_chr3:128295785-128295807_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535452 838_A1_hg19_chr5:142433047-142433069_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653320 838_A1_hg19_chr5:142433047-142433069_HEK293_edited_rep_1 838_A1_hg19_chr5:142433047-142433069_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535453 837_F12_hg19_chr9:95711879-95711901_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653322 837_F12_hg19_chr9:95711879-95711901_HEK293_control_rep_1 837_F12_hg19_chr9:95711879-95711901_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535454 837_G12_hg19_chr22:38483674-38483696_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653319 837_G12_hg19_chr22:38483674-38483696_HEK293_control_rep_1 837_G12_hg19_chr22:38483674-38483696_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535455 838_G10_hg19_chr22:46954109-46954131_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653323 838_G10_hg19_chr22:46954109-46954131_HEK293_edited_rep_1 838_G10_hg19_chr22:46954109-46954131_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535456 838_F10_hg19_chr1:154801598-154801620_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653327 838_F10_hg19_chr1:154801598-154801620_HEK293_edited_rep_1 838_F10_hg19_chr1:154801598-154801620_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535457 838_A11_hg19_chr11:68201475-68201497_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653325 838_A11_hg19_chr11:68201475-68201497_HEK293_edited_rep_1 838_A11_hg19_chr11:68201475-68201497_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535458 837_C12_hg19_chr1:113235335-113235357_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653324 837_C12_hg19_chr1:113235335-113235357_HEK293_control_rep_1 837_C12_hg19_chr1:113235335-113235357_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535459 836_B5_hg19_chr17:34883889-34883911_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653326 836_B5_hg19_chr17:34883889-34883911_HEK293_control_rep_1 836_B5_hg19_chr17:34883889-34883911_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535460 836_C5_hg19_chr1:6529315-6529337_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653328 836_C5_hg19_chr1:6529315-6529337_HEK293_control_rep_1 836_C5_hg19_chr1:6529315-6529337_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535461 838_E10_hg19_chr12:48218221-48218243_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653333 838_E10_hg19_chr12:48218221-48218243_HEK293_edited_rep_1 838_E10_hg19_chr12:48218221-48218243_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535462 838_D10_hg19_chr10:105830316-105830338_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653330 838_D10_hg19_chr10:105830316-105830338_HEK293_edited_rep_1 838_D10_hg19_chr10:105830316-105830338_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535463 836_F4_hg19_chr17:80529816-80529838_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653332 836_F4_hg19_chr17:80529816-80529838_HEK293_control_rep_1 836_F4_hg19_chr17:80529816-80529838_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535464 836_G4_hg19_chr10:133014883-133014905_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653331 836_G4_hg19_chr10:133014883-133014905_HEK293_control_rep_1 836_G4_hg19_chr10:133014883-133014905_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535465 836_H4_hg19_chr3:48606566-48606588_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653329 836_H4_hg19_chr3:48606566-48606588_HEK293_control_rep_1 836_H4_hg19_chr3:48606566-48606588_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535466 836_A5_hg19_chr8:23409382-23409404_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653340 836_A5_hg19_chr8:23409382-23409404_HEK293_control_rep_1 836_A5_hg19_chr8:23409382-23409404_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535467 836_B4_hg19_chr18:47549368-47549390_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653334 836_B4_hg19_chr18:47549368-47549390_HEK293_control_rep_1 836_B4_hg19_chr18:47549368-47549390_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535468 836_C4_hg19_chr10:102879400-102879422_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653339 836_C4_hg19_chr10:102879400-102879422_HEK293_control_rep_1 836_C4_hg19_chr10:102879400-102879422_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535469 836_D4_hg19_chr2:75874071-75874093_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653336 836_D4_hg19_chr2:75874071-75874093_HEK293_control_rep_1 836_D4_hg19_chr2:75874071-75874093_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535470 836_E4_hg19_chr14:103398376-103398398_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653341 836_E4_hg19_chr14:103398376-103398398_HEK293_control_rep_1 836_E4_hg19_chr14:103398376-103398398_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535471 832_F1_hg38_chr19:35056024-35056046_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653338 832_F1_hg38_chr19:35056024-35056046_HEK293_edited_rep_1 832_F1_hg38_chr19:35056024-35056046_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535472 832_G1_hg38_chr19:38313798-38313820_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653335 832_G1_hg38_chr19:38313798-38313820_HEK293_edited_rep_1 832_G1_hg38_chr19:38313798-38313820_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535473 834_B4_hg38_chr6:4044021-4044043_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653337 834_B4_hg38_chr6:4044021-4044043_HEK293_edited_rep_1 834_B4_hg38_chr6:4044021-4044043_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535474 834_C4_hg38_chr6:4160952-4160974_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653343 834_C4_hg38_chr6:4160952-4160974_HEK293_edited_rep_1 834_C4_hg38_chr6:4160952-4160974_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535475 832_B1_hg38_chr19:33111776-33111798_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653344 832_B1_hg38_chr19:33111776-33111798_HEK293_edited_rep_1 832_B1_hg38_chr19:33111776-33111798_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535476 832_C1_hg38_chr19:33201367-33201389_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653342 832_C1_hg38_chr19:33201367-33201389_HEK293_edited_rep_1 832_C1_hg38_chr19:33201367-33201389_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535477 831_H12_hg38_chr19:30359074-30359096_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653348 831_H12_hg38_chr19:30359074-30359096_HEK293_edited_rep_1 831_H12_hg38_chr19:30359074-30359096_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535478 832_A1_hg38_chr19:31257046-31257068_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653347 832_A1_hg38_chr19:31257046-31257068_HEK293_edited_rep_1 832_A1_hg38_chr19:31257046-31257068_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535479 834_D3_hg38_chr5:171671114-171671136_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653345 834_D3_hg38_chr5:171671114-171671136_HEK293_edited_rep_1 834_D3_hg38_chr5:171671114-171671136_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535480 834_E3_hg38_chr5:173500728-173500750_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653346 834_E3_hg38_chr5:173500728-173500750_HEK293_edited_rep_1 834_E3_hg38_chr5:173500728-173500750_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535481 834_B3_hg38_chr5:170175663-170175685_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653442 834_B3_hg38_chr5:170175663-170175685_HEK293_edited_rep_1 834_B3_hg38_chr5:170175663-170175685_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535482 834_C3_hg38_chr5:170875369-170875391_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653349 834_C3_hg38_chr5:170875369-170875391_HEK293_edited_rep_1 834_C3_hg38_chr5:170875369-170875391_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535483 834_H3_hg38_chr6:2428676-2428698_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653350 834_H3_hg38_chr6:2428676-2428698_HEK293_edited_rep_1 834_H3_hg38_chr6:2428676-2428698_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535484 834_A4_hg38_chr6:3131736-3131758_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653351 834_A4_hg38_chr6:3131736-3131758_HEK293_edited_rep_1 834_A4_hg38_chr6:3131736-3131758_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535485 834_F3_hg38_chr5:180226814-180226836_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653352 834_F3_hg38_chr5:180226814-180226836_HEK293_edited_rep_1 834_F3_hg38_chr5:180226814-180226836_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535486 834_G3_hg38_chr6:1131157-1131179_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653353 834_G3_hg38_chr6:1131157-1131179_HEK293_edited_rep_1 834_G3_hg38_chr6:1131157-1131179_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535487 838_H10_hg19_chr2:43201029-43201051_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653355 838_H10_hg19_chr2:43201029-43201051_HEK293_edited_rep_1 838_H10_hg19_chr2:43201029-43201051_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535488 840_E8_hg19_chr3:9271305-9271327_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653354 840_E8_hg19_chr3:9271305-9271327_HEK293_edited_rep_1 840_E8_hg19_chr3:9271305-9271327_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535489 840_D8_hg19_chr17:33185175-33185197_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653356 840_D8_hg19_chr17:33185175-33185197_HEK293_edited_rep_1 840_D8_hg19_chr17:33185175-33185197_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535490 840_C8_hg19_chr14:103273237-103273259_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653357 840_C8_hg19_chr14:103273237-103273259_HEK293_edited_rep_1 840_C8_hg19_chr14:103273237-103273259_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535491 838_C10_hg19_chr14:88659440-88659462_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653360 838_C10_hg19_chr14:88659440-88659462_HEK293_edited_rep_1 838_C10_hg19_chr14:88659440-88659462_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535492 840_A8_hg19_chr4:2294117-2294139_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653364 840_A8_hg19_chr4:2294117-2294139_HEK293_edited_rep_1 840_A8_hg19_chr4:2294117-2294139_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535493 840_H7_hg19_chr1:175846343-175846365_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653361 840_H7_hg19_chr1:175846343-175846365_HEK293_edited_rep_1 840_H7_hg19_chr1:175846343-175846365_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535494 840_G7_hg19_chr1:12625319-12625341_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653363 840_G7_hg19_chr1:12625319-12625341_HEK293_edited_rep_1 840_G7_hg19_chr1:12625319-12625341_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535495 840_F7_hg19_chr1:41235601-41235623_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653365 840_F7_hg19_chr1:41235601-41235623_HEK293_edited_rep_1 840_F7_hg19_chr1:41235601-41235623_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535496 840_E7_hg19_chr1:21928481-21928503_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653362 840_E7_hg19_chr1:21928481-21928503_HEK293_edited_rep_1 840_E7_hg19_chr1:21928481-21928503_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535497 838_B10_hg19_chr1:32043285-32043307_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653366 838_B10_hg19_chr1:32043285-32043307_HEK293_edited_rep_1 838_B10_hg19_chr1:32043285-32043307_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535498 831_D4_hg38_chr16:8669350-8669372_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653368 831_D4_hg38_chr16:8669350-8669372_HEK293_edited_rep_1 831_D4_hg38_chr16:8669350-8669372_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535499 831_E4_hg38_chr16:11086318-11086340_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653370 831_E4_hg38_chr16:11086318-11086340_HEK293_edited_rep_1 831_E4_hg38_chr16:11086318-11086340_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535500 831_B4_hg38_chr16:5952778-5952800_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653367 831_B4_hg38_chr16:5952778-5952800_HEK293_edited_rep_1 831_B4_hg38_chr16:5952778-5952800_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535501 831_C4_hg38_chr16:6246714-6246736_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653373 831_C4_hg38_chr16:6246714-6246736_HEK293_edited_rep_1 831_C4_hg38_chr16:6246714-6246736_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535502 831_H4_hg38_chr16:27195739-27195761_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653369 831_H4_hg38_chr16:27195739-27195761_HEK293_edited_rep_1 831_H4_hg38_chr16:27195739-27195761_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535503 835_D3_hg19_chr5:38575912-38575934_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653372 835_D3_hg19_chr5:38575912-38575934_HEK293_control_rep_1 835_D3_hg19_chr5:38575912-38575934_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535504 831_F4_hg38_chr16:23927248-23927270_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653371 831_F4_hg38_chr16:23927248-23927270_HEK293_edited_rep_1 831_F4_hg38_chr16:23927248-23927270_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535505 831_G4_hg38_chr16:26816708-26816730_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653375 831_G4_hg38_chr16:26816708-26816730_HEK293_edited_rep_1 831_G4_hg38_chr16:26816708-26816730_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535506 831_B5_hg38_chr16:49693538-49693560_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653374 831_B5_hg38_chr16:49693538-49693560_HEK293_edited_rep_1 831_B5_hg38_chr16:49693538-49693560_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535507 834_H1_hg38_chr5:133898698-133898720_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653376 834_H1_hg38_chr5:133898698-133898720_HEK293_edited_rep_1 834_H1_hg38_chr5:133898698-133898720_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535508 839_D5_hg19_chr20:42338563-42338585_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653377 839_D5_hg19_chr20:42338563-42338585_HEK293_edited_rep_1 839_D5_hg19_chr20:42338563-42338585_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535509 839_H7_hg19_chr6:166184438-166184460_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653378 839_H7_hg19_chr6:166184438-166184460_HEK293_edited_rep_1 839_H7_hg19_chr6:166184438-166184460_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535510 839_A8_hg19_chr3:85611236-85611258_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653459 839_A8_hg19_chr3:85611236-85611258_HEK293_edited_rep_1 839_A8_hg19_chr3:85611236-85611258_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535511 839_E5_hg19_chr10:21056679-21056701_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653379 839_E5_hg19_chr10:21056679-21056701_HEK293_edited_rep_1 839_E5_hg19_chr10:21056679-21056701_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535512 840_C10_hg19_chr13:31618284-31618306_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653380 840_C10_hg19_chr13:31618284-31618306_HEK293_edited_rep_1 840_C10_hg19_chr13:31618284-31618306_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535513 839_H6_hg19_chr7:2144143-2144165_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653381 839_H6_hg19_chr7:2144143-2144165_HEK293_edited_rep_1 839_H6_hg19_chr7:2144143-2144165_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535514 837_G4_hg19_chr9:42802556-42802578_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653460 837_G4_hg19_chr9:42802556-42802578_HEK293_control_rep_1 837_G4_hg19_chr9:42802556-42802578_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535515 839_B7_hg19_chr2:29617973-29617995_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653382 839_B7_hg19_chr2:29617973-29617995_HEK293_edited_rep_1 839_B7_hg19_chr2:29617973-29617995_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535516 839_C7_hg19_chr6:41529749-41529771_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653384 839_C7_hg19_chr6:41529749-41529771_HEK293_edited_rep_1 839_C7_hg19_chr6:41529749-41529771_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535517 839_D7_hg19_chr11:120064102-120064124_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653383 839_D7_hg19_chr11:120064102-120064124_HEK293_edited_rep_1 839_D7_hg19_chr11:120064102-120064124_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535518 839_E7_hg19_chr8:22635581-22635603_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653389 839_E7_hg19_chr8:22635581-22635603_HEK293_edited_rep_1 839_E7_hg19_chr8:22635581-22635603_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535519 839_F7_hg19_chr7:96623460-96623482_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653390 839_F7_hg19_chr7:96623460-96623482_HEK293_edited_rep_1 839_F7_hg19_chr7:96623460-96623482_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535520 837_F4_hg19_chr16:4749181-4749203_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653387 837_F4_hg19_chr16:4749181-4749203_HEK293_control_rep_1 837_F4_hg19_chr16:4749181-4749203_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535521 838_C11_hg19_chr20:20309857-20309879_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653386 838_C11_hg19_chr20:20309857-20309879_HEK293_edited_rep_1 838_C11_hg19_chr20:20309857-20309879_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535522 1734_G7_hg38_chr6:117602562-117602584_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653388 1734_G7_hg38_chr6:117602562-117602584_HEK293_control_rep_1 1734_G7_hg38_chr6:117602562-117602584_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535523 838_B11_hg19_chr19:2187414-2187436_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653385 838_B11_hg19_chr19:2187414-2187436_HEK293_edited_rep_1 838_B11_hg19_chr19:2187414-2187436_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535524 837_F6_hg19_chr14:23520110-23520132_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653391 837_F6_hg19_chr14:23520110-23520132_HEK293_control_rep_1 837_F6_hg19_chr14:23520110-23520132_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535525 837_G6_hg19_chr12:9730868-9730890_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653393 837_G6_hg19_chr12:9730868-9730890_HEK293_control_rep_1 837_G6_hg19_chr12:9730868-9730890_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535526 837_D6_hg19_chr5:2410480-2410502_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653392 837_D6_hg19_chr5:2410480-2410502_HEK293_control_rep_1 837_D6_hg19_chr5:2410480-2410502_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535527 837_E6_hg19_chr11:119675039-119675061_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653468 837_E6_hg19_chr11:119675039-119675061_HEK293_control_rep_1 837_E6_hg19_chr11:119675039-119675061_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535528 837_B6_hg19_chr1:181453844-181453866_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653394 837_B6_hg19_chr1:181453844-181453866_HEK293_control_rep_1 837_B6_hg19_chr1:181453844-181453866_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535529 834_A3_hg38_chr5:162206141-162206163_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653395 834_A3_hg38_chr5:162206141-162206163_HEK293_edited_rep_1 834_A3_hg38_chr5:162206141-162206163_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535530 837_H5_hg19_chr1:51754769-51754791_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653397 837_H5_hg19_chr1:51754769-51754791_HEK293_control_rep_1 837_H5_hg19_chr1:51754769-51754791_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535531 837_A6_hg19_chr1:95200617-95200639_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653396 837_A6_hg19_chr1:95200617-95200639_HEK293_control_rep_1 837_A6_hg19_chr1:95200617-95200639_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535532 834_H2_hg38_chr5:159245797-159245819_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653398 834_H2_hg38_chr5:159245797-159245819_HEK293_edited_rep_1 834_H2_hg38_chr5:159245797-159245819_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535533 839_D4_hg19_chr2:75874071-75874093_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653401 839_D4_hg19_chr2:75874071-75874093_HEK293_edited_rep_1 839_D4_hg19_chr2:75874071-75874093_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535534 837_H6_hg19_chr19:50161823-50161845_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653400 837_H6_hg19_chr19:50161823-50161845_HEK293_control_rep_1 837_H6_hg19_chr19:50161823-50161845_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535535 837_A7_hg19_chr3:13525177-13525199_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653403 837_A7_hg19_chr3:13525177-13525199_HEK293_control_rep_1 837_A7_hg19_chr3:13525177-13525199_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535536 834_H12_hg38_chr7:150472242-150472264_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653402 834_H12_hg38_chr7:150472242-150472264_HEK293_edited_rep_1 834_H12_hg38_chr7:150472242-150472264_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535537 833_D6_hg38_chr3:50289820-50289842_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653399 833_D6_hg38_chr3:50289820-50289842_HEK293_edited_rep_1 833_D6_hg38_chr3:50289820-50289842_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535538 833_G6_hg38_chr3:53250412-53250434_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653404 833_G6_hg38_chr3:53250412-53250434_HEK293_edited_rep_1 833_G6_hg38_chr3:53250412-53250434_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535539 833_F6_hg38_chr3:53067538-53067560_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653408 833_F6_hg38_chr3:53067538-53067560_HEK293_edited_rep_1 833_F6_hg38_chr3:53067538-53067560_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535540 833_A7_hg38_chr3:57150158-57150180_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653406 833_A7_hg38_chr3:57150158-57150180_HEK293_edited_rep_1 833_A7_hg38_chr3:57150158-57150180_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535541 833_H6_hg38_chr3:53971683-53971705_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653407 833_H6_hg38_chr3:53971683-53971705_HEK293_edited_rep_1 833_H6_hg38_chr3:53971683-53971705_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535542 833_C7_hg38_chr3:58176145-58176167_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653405 833_C7_hg38_chr3:58176145-58176167_HEK293_edited_rep_1 833_C7_hg38_chr3:58176145-58176167_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535543 833_B7_hg38_chr3:57530771-57530793_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653409 833_B7_hg38_chr3:57530771-57530793_HEK293_edited_rep_1 833_B7_hg38_chr3:57530771-57530793_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535544 833_E7_hg38_chr3:67480548-67480570_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653410 833_E7_hg38_chr3:67480548-67480570_HEK293_edited_rep_1 833_E7_hg38_chr3:67480548-67480570_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535545 833_D7_hg38_chr3:58897931-58897953_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653413 833_D7_hg38_chr3:58897931-58897953_HEK293_edited_rep_1 833_D7_hg38_chr3:58897931-58897953_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535546 834_B12_hg38_chr7:131878631-131878653_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653411 834_B12_hg38_chr7:131878631-131878653_HEK293_edited_rep_1 834_B12_hg38_chr7:131878631-131878653_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535547 834_C12_hg38_chr7:132609447-132609469_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653412 834_C12_hg38_chr7:132609447-132609469_HEK293_edited_rep_1 834_C12_hg38_chr7:132609447-132609469_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535548 834_D12_hg38_chr7:132791700-132791722_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653414 834_D12_hg38_chr7:132791700-132791722_HEK293_edited_rep_1 834_D12_hg38_chr7:132791700-132791722_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535549 834_E12_hg38_chr7:142130488-142130510_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653415 834_E12_hg38_chr7:142130488-142130510_HEK293_edited_rep_1 834_E12_hg38_chr7:142130488-142130510_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535550 834_F12_hg38_chr7:147685843-147685865_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653416 834_F12_hg38_chr7:147685843-147685865_HEK293_edited_rep_1 834_F12_hg38_chr7:147685843-147685865_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535551 834_G12_hg38_chr7:148551750-148551772_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653417 834_G12_hg38_chr7:148551750-148551772_HEK293_edited_rep_1 834_G12_hg38_chr7:148551750-148551772_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535552 840_H3_hg19_chr9:136892384-136892406_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653418 840_H3_hg19_chr9:136892384-136892406_HEK293_edited_rep_1 840_H3_hg19_chr9:136892384-136892406_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535553 840_A4_hg19_chr20:980410-980432_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653419 840_A4_hg19_chr20:980410-980432_HEK293_edited_rep_1 840_A4_hg19_chr20:980410-980432_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535554 840_F3_hg19_chr4:1224010-1224032_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653420 840_F3_hg19_chr4:1224010-1224032_HEK293_edited_rep_1 840_F3_hg19_chr4:1224010-1224032_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535555 840_G3_hg19_chrX:48917318-48917340_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653421 840_G3_hg19_chrX:48917318-48917340_HEK293_edited_rep_1 840_G3_hg19_chrX:48917318-48917340_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535556 840_D4_hg19_chr16:50344049-50344071_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653423 840_D4_hg19_chr16:50344049-50344071_HEK293_edited_rep_1 840_D4_hg19_chr16:50344049-50344071_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535557 840_E4_hg19_chr8:6664341-6664363_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653424 840_E4_hg19_chr8:6664341-6664363_HEK293_edited_rep_1 840_E4_hg19_chr8:6664341-6664363_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535558 840_B4_hg19_chr1:31859278-31859300_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653422 840_B4_hg19_chr1:31859278-31859300_HEK293_edited_rep_1 840_B4_hg19_chr1:31859278-31859300_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535559 840_C4_hg19_chr19:58920698-58920720_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653425 840_C4_hg19_chr19:58920698-58920720_HEK293_edited_rep_1 840_C4_hg19_chr19:58920698-58920720_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535560 1734_C2_hg38_chr15:45535695-45535717_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653426 1734_C2_hg38_chr15:45535695-45535717_HEK293_control_rep_1 1734_C2_hg38_chr15:45535695-45535717_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535561 1734_B2_hg38_chr10:101119643-101119665_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653429 1734_B2_hg38_chr10:101119643-101119665_HEK293_control_rep_1 1734_B2_hg38_chr10:101119643-101119665_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535562 840_F4_hg19_chr16:4749181-4749203_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653428 840_F4_hg19_chr16:4749181-4749203_HEK293_edited_rep_1 840_F4_hg19_chr16:4749181-4749203_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535563 840_G4_hg19_chr9:42802556-42802578_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653427 840_G4_hg19_chr9:42802556-42802578_HEK293_edited_rep_1 840_G4_hg19_chr9:42802556-42802578_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535564 1734_G1_hg38_chr5:858350-858372_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653430 1734_G1_hg38_chr5:858350-858372_HEK293_control_rep_1 1734_G1_hg38_chr5:858350-858372_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535565 1734_F1_hg38_chr6:53335290-53335312_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653431 1734_F1_hg38_chr6:53335290-53335312_HEK293_control_rep_1 1734_F1_hg38_chr6:53335290-53335312_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535566 1734_A2_hg38_chr11:61343765-61343787_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653434 1734_A2_hg38_chr11:61343765-61343787_HEK293_control_rep_1 1734_A2_hg38_chr11:61343765-61343787_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535567 1734_H1_hg38_chr8:143802950-143802972_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653435 1734_H1_hg38_chr8:143802950-143802972_HEK293_control_rep_1 1734_H1_hg38_chr8:143802950-143802972_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535568 1734_C3_hg38_chr10:119439169-119439191_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653432 1734_C3_hg38_chr10:119439169-119439191_HEK293_control_rep_1 1734_C3_hg38_chr10:119439169-119439191_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535569 836_G7_hg19_chr5:53554410-53554432_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653433 836_G7_hg19_chr5:53554410-53554432_HEK293_control_rep_1 836_G7_hg19_chr5:53554410-53554432_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535570 836_F7_hg19_chr7:96623460-96623482_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653436 836_F7_hg19_chr7:96623460-96623482_HEK293_control_rep_1 836_F7_hg19_chr7:96623460-96623482_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535571 836_C7_hg19_chr6:41529749-41529771_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653438 836_C7_hg19_chr6:41529749-41529771_HEK293_control_rep_1 836_C7_hg19_chr6:41529749-41529771_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535572 836_B7_hg19_chr2:29617973-29617995_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653437 836_B7_hg19_chr2:29617973-29617995_HEK293_control_rep_1 836_B7_hg19_chr2:29617973-29617995_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535573 836_E7_hg19_chr8:22635581-22635603_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653440 836_E7_hg19_chr8:22635581-22635603_HEK293_control_rep_1 836_E7_hg19_chr8:22635581-22635603_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535574 836_D7_hg19_chr11:120064102-120064124_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653441 836_D7_hg19_chr11:120064102-120064124_HEK293_control_rep_1 836_D7_hg19_chr11:120064102-120064124_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535575 836_G6_hg19_chr8:11553531-11553553_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653439 836_G6_hg19_chr8:11553531-11553553_HEK293_control_rep_1 836_G6_hg19_chr8:11553531-11553553_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535576 836_F6_hg19_chr9:123505399-123505421_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653443 836_F6_hg19_chr9:123505399-123505421_HEK293_control_rep_1 836_F6_hg19_chr9:123505399-123505421_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535577 836_A7_hg19_chr4:185181290-185181312_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653444 836_A7_hg19_chr4:185181290-185181312_HEK293_control_rep_1 836_A7_hg19_chr4:185181290-185181312_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535578 836_H6_hg19_chr7:2144143-2144165_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653446 836_H6_hg19_chr7:2144143-2144165_HEK293_control_rep_1 836_H6_hg19_chr7:2144143-2144165_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535579 838_F12_hg19_chr10:69247872-69247894_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653491 838_F12_hg19_chr10:69247872-69247894_HEK293_edited_rep_1 838_F12_hg19_chr10:69247872-69247894_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535580 1734_B5_hg38_chr7:16059742-16059764_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653445 1734_B5_hg38_chr7:16059742-16059764_HEK293_control_rep_1 1734_B5_hg38_chr7:16059742-16059764_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535581 832_H8_hg38_chr2:238930396-238930418_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653447 832_H8_hg38_chr2:238930396-238930418_HEK293_edited_rep_1 832_H8_hg38_chr2:238930396-238930418_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535582 832_A9_hg38_chr20:2750207-2750229_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653451 832_A9_hg38_chr20:2750207-2750229_HEK293_edited_rep_1 832_A9_hg38_chr20:2750207-2750229_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535583 832_B9_hg38_chr20:5030906-5030928_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653450 832_B9_hg38_chr20:5030906-5030928_HEK293_edited_rep_1 832_B9_hg38_chr20:5030906-5030928_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535584 832_C9_hg38_chr20:7855454-7855476_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653448 832_C9_hg38_chr20:7855454-7855476_HEK293_edited_rep_1 832_C9_hg38_chr20:7855454-7855476_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535585 832_D8_hg38_chr2:230684136-230684158_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653452 832_D8_hg38_chr2:230684136-230684158_HEK293_edited_rep_1 832_D8_hg38_chr2:230684136-230684158_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535586 832_E8_hg38_chr2:234484904-234484926_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653449 832_E8_hg38_chr2:234484904-234484926_HEK293_edited_rep_1 832_E8_hg38_chr2:234484904-234484926_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535587 832_F8_hg38_chr2:236856593-236856615_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653492 832_F8_hg38_chr2:236856593-236856615_HEK293_edited_rep_1 832_F8_hg38_chr2:236856593-236856615_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535588 832_G8_hg38_chr2:238004621-238004643_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653453 832_G8_hg38_chr2:238004621-238004643_HEK293_edited_rep_1 832_G8_hg38_chr2:238004621-238004643_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535589 840_H9_hg19_chr17:5704930-5704952_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653494 840_H9_hg19_chr17:5704930-5704952_HEK293_edited_rep_1 840_H9_hg19_chr17:5704930-5704952_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535590 832_D9_hg38_chr20:10507388-10507410_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653454 832_D9_hg38_chr20:10507388-10507410_HEK293_edited_rep_1 832_D9_hg38_chr20:10507388-10507410_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535591 832_E9_hg38_chr20:13379564-13379586_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653496 832_E9_hg38_chr20:13379564-13379586_HEK293_edited_rep_1 832_E9_hg38_chr20:13379564-13379586_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535592 839_C4_hg19_chr10:102879400-102879422_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653455 839_C4_hg19_chr10:102879400-102879422_HEK293_edited_rep_1 839_C4_hg19_chr10:102879400-102879422_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535593 839_B4_hg19_chr18:47549368-47549390_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653505 839_B4_hg19_chr18:47549368-47549390_HEK293_edited_rep_1 839_B4_hg19_chr18:47549368-47549390_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535594 832_E1_hg38_chr19:33715354-33715376_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653457 832_E1_hg38_chr19:33715354-33715376_HEK293_edited_rep_1 832_E1_hg38_chr19:33715354-33715376_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535595 839_C3_hg19_chr11:117711639-117711661_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653456 839_C3_hg19_chr11:117711639-117711661_HEK293_edited_rep_1 839_C3_hg19_chr11:117711639-117711661_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535596 839_B3_hg19_chr15:63199386-63199408_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653458 839_B3_hg19_chr15:63199386-63199408_HEK293_edited_rep_1 839_B3_hg19_chr15:63199386-63199408_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535597 839_E3_hg19_chr3:148674153-148674175_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653501 839_E3_hg19_chr3:148674153-148674175_HEK293_edited_rep_1 839_E3_hg19_chr3:148674153-148674175_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535598 839_D3_hg19_chr6:155472166-155472188_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653462 839_D3_hg19_chr6:155472166-155472188_HEK293_edited_rep_1 839_D3_hg19_chr6:155472166-155472188_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535599 839_G3_hg19_chr22:23427175-23427197_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653461 839_G3_hg19_chr22:23427175-23427197_HEK293_edited_rep_1 839_G3_hg19_chr22:23427175-23427197_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535600 839_G1_hg19_chr3:172018373-172018395_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653463 839_G1_hg19_chr3:172018373-172018395_HEK293_edited_rep_1 839_G1_hg19_chr3:172018373-172018395_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535601 839_A4_hg19_chr10:133774386-133774408_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653508 839_A4_hg19_chr10:133774386-133774408_HEK293_edited_rep_1 839_A4_hg19_chr10:133774386-133774408_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535602 839_H3_hg19_chr11:46498098-46498120_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653464 839_H3_hg19_chr11:46498098-46498120_HEK293_edited_rep_1 839_H3_hg19_chr11:46498098-46498120_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535603 835_B11_hg19_chr19:2187414-2187436_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653465 835_B11_hg19_chr19:2187414-2187436_HEK293_control_rep_1 835_B11_hg19_chr19:2187414-2187436_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535604 835_C11_hg19_chr20:20309857-20309879_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653466 835_C11_hg19_chr20:20309857-20309879_HEK293_control_rep_1 835_C11_hg19_chr20:20309857-20309879_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535605 835_D11_hg19_chr16:17399046-17399068_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653467 835_D11_hg19_chr16:17399046-17399068_HEK293_control_rep_1 835_D11_hg19_chr16:17399046-17399068_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535606 835_E11_hg19_chr10:132580816-132580838_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653676 835_E11_hg19_chr10:132580816-132580838_HEK293_control_rep_1 835_E11_hg19_chr10:132580816-132580838_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535607 835_F11_hg19_chr1:48417872-48417894_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653469 835_F11_hg19_chr1:48417872-48417894_HEK293_control_rep_1 835_F11_hg19_chr1:48417872-48417894_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535608 835_G11_hg19_chr17:26958788-26958810_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653470 835_G11_hg19_chr17:26958788-26958810_HEK293_control_rep_1 835_G11_hg19_chr17:26958788-26958810_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535609 835_H11_hg19_chr8:144885120-144885142_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653471 835_H11_hg19_chr8:144885120-144885142_HEK293_control_rep_1 835_H11_hg19_chr8:144885120-144885142_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535610 835_A12_hg19_chr10:105463950-105463972_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653472 835_A12_hg19_chr10:105463950-105463972_HEK293_control_rep_1 835_A12_hg19_chr10:105463950-105463972_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535611 835_B12_hg19_chr22:17529539-17529561_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653517 835_B12_hg19_chr22:17529539-17529561_HEK293_control_rep_1 835_B12_hg19_chr22:17529539-17529561_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535612 835_C12_hg19_chr19:4817649-4817671_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653519 835_C12_hg19_chr19:4817649-4817671_HEK293_control_rep_1 835_C12_hg19_chr19:4817649-4817671_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535613 1734_D7_hg38_chr20:999767-999789_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653473 1734_D7_hg38_chr20:999767-999789_HEK293_control_rep_1 1734_D7_hg38_chr20:999767-999789_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535614 838_A8_hg19_chr19:10753884-10753906_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653474 838_A8_hg19_chr19:10753884-10753906_HEK293_edited_rep_1 838_A8_hg19_chr19:10753884-10753906_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535615 839_B2_hg19_chr11:61111237-61111259_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653475 839_B2_hg19_chr11:61111237-61111259_HEK293_edited_rep_1 839_B2_hg19_chr11:61111237-61111259_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535616 840_H8_hg19_chr17:27303632-27303654_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653479 840_H8_hg19_chr17:27303632-27303654_HEK293_edited_rep_1 840_H8_hg19_chr17:27303632-27303654_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535617 840_F5_hg19_chr3:48562425-48562447_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653531 840_F5_hg19_chr3:48562425-48562447_HEK293_edited_rep_1 840_F5_hg19_chr3:48562425-48562447_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535618 839_F1_hg19_chr20:45321341-45321363_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653477 839_F1_hg19_chr20:45321341-45321363_HEK293_edited_rep_1 839_F1_hg19_chr20:45321341-45321363_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535619 1734_H8_hg38_chr1:6469255-6469277_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653478 1734_H8_hg38_chr1:6469255-6469277_HEK293_control_rep_1 1734_H8_hg38_chr1:6469255-6469277_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535620 1734_A9_hg38_chr6:34471947-34471969_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653480 1734_A9_hg38_chr6:34471947-34471969_HEK293_control_rep_1 1734_A9_hg38_chr6:34471947-34471969_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535621 832_H1_hg38_chr19:41303793-41303815_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653481 832_H1_hg38_chr19:41303793-41303815_HEK293_edited_rep_1 832_H1_hg38_chr19:41303793-41303815_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535622 833_E6_hg38_chr3:52303542-52303564_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653482 833_E6_hg38_chr3:52303542-52303564_HEK293_edited_rep_1 833_E6_hg38_chr3:52303542-52303564_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535623 1734_B8_hg38_chr8:23551869-23551891_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653483 1734_B8_hg38_chr8:23551869-23551891_HEK293_control_rep_1 1734_B8_hg38_chr8:23551869-23551891_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535624 1734_C8_hg38_chr1:112692713-112692735_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653484 1734_C8_hg38_chr1:112692713-112692735_HEK293_control_rep_1 1734_C8_hg38_chr1:112692713-112692735_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535625 1734_H7_hg38_chr16:1103458-1103480_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653485 1734_H7_hg38_chr16:1103458-1103480_HEK293_control_rep_1 1734_H7_hg38_chr16:1103458-1103480_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535626 832_A2_hg38_chr19:41405948-41405970_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653486 832_A2_hg38_chr19:41405948-41405970_HEK293_edited_rep_1 832_A2_hg38_chr19:41405948-41405970_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535627 838_H7_hg19_chr8:21752718-21752740_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653487 838_H7_hg19_chr8:21752718-21752740_HEK293_edited_rep_1 838_H7_hg19_chr8:21752718-21752740_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535628 840_B12_hg19_chr1:942044-942066_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653488 840_B12_hg19_chr1:942044-942066_HEK293_edited_rep_1 840_B12_hg19_chr1:942044-942066_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535629 1734_D8_hg38_chr2:27380970-27380992_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653489 1734_D8_hg38_chr2:27380970-27380992_HEK293_control_rep_1 1734_D8_hg38_chr2:27380970-27380992_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535630 1734_E8_hg38_chr5:38575810-38575832_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653490 1734_E8_hg38_chr5:38575810-38575832_HEK293_control_rep_1 1734_E8_hg38_chr5:38575810-38575832_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535631 837_A9_hg19_chr9:92206502-92206524_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653493 837_A9_hg19_chr9:92206502-92206524_HEK293_control_rep_1 837_A9_hg19_chr9:92206502-92206524_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535632 837_H8_hg19_chr17:27303632-27303654_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653495 837_H8_hg19_chr17:27303632-27303654_HEK293_control_rep_1 837_H8_hg19_chr17:27303632-27303654_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535633 837_C9_hg19_chr4:22173606-22173628_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653497 837_C9_hg19_chr4:22173606-22173628_HEK293_control_rep_1 837_C9_hg19_chr4:22173606-22173628_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535634 837_B9_hg19_chr2:60390559-60390581_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653498 837_B9_hg19_chr2:60390559-60390581_HEK293_control_rep_1 837_B9_hg19_chr2:60390559-60390581_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535635 837_E8_hg19_chr3:9271305-9271327_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653500 837_E8_hg19_chr3:9271305-9271327_HEK293_control_rep_1 837_E8_hg19_chr3:9271305-9271327_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535636 837_D8_hg19_chr17:33185175-33185197_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653499 837_D8_hg19_chr17:33185175-33185197_HEK293_control_rep_1 837_D8_hg19_chr17:33185175-33185197_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535637 837_G8_hg19_chr15:45827893-45827915_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653503 837_G8_hg19_chr15:45827893-45827915_HEK293_control_rep_1 837_G8_hg19_chr15:45827893-45827915_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535638 837_F8_hg19_chr13:112066609-112066631_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653502 837_F8_hg19_chr13:112066609-112066631_HEK293_control_rep_1 837_F8_hg19_chr13:112066609-112066631_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535639 837_E9_hg19_chr10:99272320-99272342_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653504 837_E9_hg19_chr10:99272320-99272342_HEK293_control_rep_1 837_E9_hg19_chr10:99272320-99272342_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535640 837_D9_hg19_chr7:137602541-137602563_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653507 837_D9_hg19_chr7:137602541-137602563_HEK293_control_rep_1 837_D9_hg19_chr7:137602541-137602563_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535641 1734_C6_hg38_chr2:75646945-75646967_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653506 1734_C6_hg38_chr2:75646945-75646967_HEK293_control_rep_1 1734_C6_hg38_chr2:75646945-75646967_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535642 832_C3_hg38_chr2:26578866-26578888_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653509 832_C3_hg38_chr2:26578866-26578888_HEK293_edited_rep_1 832_C3_hg38_chr2:26578866-26578888_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535643 832_B3_hg38_chr2:11982417-11982439_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653511 832_B3_hg38_chr2:11982417-11982439_HEK293_edited_rep_1 832_B3_hg38_chr2:11982417-11982439_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535644 832_A3_hg38_chr2:9816164-9816186_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653510 832_A3_hg38_chr2:9816164-9816186_HEK293_edited_rep_1 832_A3_hg38_chr2:9816164-9816186_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535645 832_H2_hg38_chr2:4937821-4937843_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653513 832_H2_hg38_chr2:4937821-4937843_HEK293_edited_rep_1 832_H2_hg38_chr2:4937821-4937843_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535646 832_G2_hg38_chr2:4042534-4042556_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653512 832_G2_hg38_chr2:4042534-4042556_HEK293_edited_rep_1 832_G2_hg38_chr2:4042534-4042556_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535647 832_F2_hg38_chr2:3156919-3156941_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653514 832_F2_hg38_chr2:3156919-3156941_HEK293_edited_rep_1 832_F2_hg38_chr2:3156919-3156941_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535648 832_E2_hg38_chr19:58468894-58468916_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653515 832_E2_hg38_chr19:58468894-58468916_HEK293_edited_rep_1 832_E2_hg38_chr19:58468894-58468916_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535649 832_D2_hg38_chr19:58366378-58366400_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653516 832_D2_hg38_chr19:58366378-58366400_HEK293_edited_rep_1 832_D2_hg38_chr19:58366378-58366400_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535650 832_C2_hg38_chr19:50952965-50952987_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653518 832_C2_hg38_chr19:50952965-50952987_HEK293_edited_rep_1 832_C2_hg38_chr19:50952965-50952987_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535651 832_B2_hg38_chr19:50643970-50643992_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653522 832_B2_hg38_chr19:50643970-50643992_HEK293_edited_rep_1 832_B2_hg38_chr19:50643970-50643992_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535652 834_H7_hg38_chr6:159959635-159959657_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653520 834_H7_hg38_chr6:159959635-159959657_HEK293_edited_rep_1 834_H7_hg38_chr6:159959635-159959657_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535653 834_A8_hg38_chr6:163281070-163281092_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653521 834_A8_hg38_chr6:163281070-163281092_HEK293_edited_rep_1 834_A8_hg38_chr6:163281070-163281092_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535654 840_B8_hg19_chr13:20176711-20176733_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653524 840_B8_hg19_chr13:20176711-20176733_HEK293_edited_rep_1 840_B8_hg19_chr13:20176711-20176733_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535655 834_D7_hg38_chr6:157983004-157983026_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653523 834_D7_hg38_chr6:157983004-157983026_HEK293_edited_rep_1 834_D7_hg38_chr6:157983004-157983026_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535656 834_E7_hg38_chr6:158559650-158559672_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653525 834_E7_hg38_chr6:158559650-158559672_HEK293_edited_rep_1 834_E7_hg38_chr6:158559650-158559672_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535657 834_F7_hg38_chr6:158584415-158584437_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653526 834_F7_hg38_chr6:158584415-158584437_HEK293_edited_rep_1 834_F7_hg38_chr6:158584415-158584437_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535658 834_G7_hg38_chr6:159936542-159936564_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653527 834_G7_hg38_chr6:159936542-159936564_HEK293_edited_rep_1 834_G7_hg38_chr6:159936542-159936564_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535659 834_H6_hg38_chr6:108749562-108749584_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653528 834_H6_hg38_chr6:108749562-108749584_HEK293_edited_rep_1 834_H6_hg38_chr6:108749562-108749584_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535660 834_A7_hg38_chr6:108963780-108963802_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653532 834_A7_hg38_chr6:108963780-108963802_HEK293_edited_rep_1 834_A7_hg38_chr6:108963780-108963802_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535661 834_B7_hg38_chr6:110398436-110398458_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653529 834_B7_hg38_chr6:110398436-110398458_HEK293_edited_rep_1 834_B7_hg38_chr6:110398436-110398458_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535662 834_C7_hg38_chr6:142377498-142377520_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653534 834_C7_hg38_chr6:142377498-142377520_HEK293_edited_rep_1 834_C7_hg38_chr6:142377498-142377520_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535663 840_A9_hg19_chr9:92206502-92206524_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653535 840_A9_hg19_chr9:92206502-92206524_HEK293_edited_rep_1 840_A9_hg19_chr9:92206502-92206524_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535664 840_G8_hg19_chr15:45827893-45827915_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653538 840_G8_hg19_chr15:45827893-45827915_HEK293_edited_rep_1 840_G8_hg19_chr15:45827893-45827915_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535665 840_F8_hg19_chr13:112066609-112066631_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653533 840_F8_hg19_chr13:112066609-112066631_HEK293_edited_rep_1 840_F8_hg19_chr13:112066609-112066631_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535666 831_H8_hg38_chr17:66897292-66897314_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653537 831_H8_hg38_chr17:66897292-66897314_HEK293_edited_rep_1 831_H8_hg38_chr17:66897292-66897314_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535667 831_A9_hg38_chr17:73514655-73514677_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653530 831_A9_hg38_chr17:73514655-73514677_HEK293_edited_rep_1 831_A9_hg38_chr17:73514655-73514677_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535668 840_C9_hg19_chr4:22173606-22173628_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653536 840_C9_hg19_chr4:22173606-22173628_HEK293_edited_rep_1 840_C9_hg19_chr4:22173606-22173628_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535669 840_B9_hg19_chr2:60390559-60390581_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653539 840_B9_hg19_chr2:60390559-60390581_HEK293_edited_rep_1 840_B9_hg19_chr2:60390559-60390581_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535670 840_E9_hg19_chr10:99272320-99272342_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653540 840_E9_hg19_chr10:99272320-99272342_HEK293_edited_rep_1 840_E9_hg19_chr10:99272320-99272342_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535671 840_D9_hg19_chr7:137602541-137602563_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653541 840_D9_hg19_chr7:137602541-137602563_HEK293_edited_rep_1 840_D9_hg19_chr7:137602541-137602563_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535672 831_B8_hg38_chr17:50266819-50266841_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653542 831_B8_hg38_chr17:50266819-50266841_HEK293_edited_rep_1 831_B8_hg38_chr17:50266819-50266841_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535673 831_C8_hg38_chr17:50913314-50913336_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653543 831_C8_hg38_chr17:50913314-50913336_HEK293_edited_rep_1 831_C8_hg38_chr17:50913314-50913336_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535674 831_H7_hg38_chr17:44313970-44313992_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653545 831_H7_hg38_chr17:44313970-44313992_HEK293_edited_rep_1 831_H7_hg38_chr17:44313970-44313992_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535675 831_A8_hg38_chr17:49240171-49240193_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653544 831_A8_hg38_chr17:49240171-49240193_HEK293_edited_rep_1 831_A8_hg38_chr17:49240171-49240193_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535676 831_F8_hg38_chr17:63931468-63931490_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653546 831_F8_hg38_chr17:63931468-63931490_HEK293_edited_rep_1 831_F8_hg38_chr17:63931468-63931490_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535677 831_G8_hg38_chr17:65039584-65039606_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653547 831_G8_hg38_chr17:65039584-65039606_HEK293_edited_rep_1 831_G8_hg38_chr17:65039584-65039606_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535678 831_D8_hg38_chr17:56531036-56531058_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653549 831_D8_hg38_chr17:56531036-56531058_HEK293_edited_rep_1 831_D8_hg38_chr17:56531036-56531058_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535679 831_E8_hg38_chr17:58411493-58411515_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653551 831_E8_hg38_chr17:58411493-58411515_HEK293_edited_rep_1 831_E8_hg38_chr17:58411493-58411515_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535680 840_G9_hg19_chr10:121198681-121198703_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653548 840_G9_hg19_chr10:121198681-121198703_HEK293_edited_rep_1 840_G9_hg19_chr10:121198681-121198703_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535681 840_F9_hg19_chr13:107769207-107769229_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653552 840_F9_hg19_chr13:107769207-107769229_HEK293_edited_rep_1 840_F9_hg19_chr13:107769207-107769229_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535682 831_A5_hg38_chr16:49015176-49015198_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653550 831_A5_hg38_chr16:49015176-49015198_HEK293_edited_rep_1 831_A5_hg38_chr16:49015176-49015198_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535683 834_F1_hg38_chr5:95884960-95884982_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653553 834_F1_hg38_chr5:95884960-95884982_HEK293_edited_rep_1 834_F1_hg38_chr5:95884960-95884982_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535684 834_G1_hg38_chr5:113145840-113145862_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653554 834_G1_hg38_chr5:113145840-113145862_HEK293_edited_rep_1 834_G1_hg38_chr5:113145840-113145862_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535685 836_A12_hg19_chr1:4425560-4425582_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653555 836_A12_hg19_chr1:4425560-4425582_HEK293_control_rep_1 836_A12_hg19_chr1:4425560-4425582_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535686 836_H11_hg19_chr8:144028903-144028925_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653556 836_H11_hg19_chr8:144028903-144028925_HEK293_control_rep_1 836_H11_hg19_chr8:144028903-144028925_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535687 836_C12_hg19_chr17:29171904-29171926_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653557 836_C12_hg19_chr17:29171904-29171926_HEK293_control_rep_1 836_C12_hg19_chr17:29171904-29171926_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535688 836_B12_hg19_chr7:73871682-73871704_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653558 836_B12_hg19_chr7:73871682-73871704_HEK293_control_rep_1 836_B12_hg19_chr7:73871682-73871704_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535689 836_E12_hg19_chr15:73460913-73460935_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653560 836_E12_hg19_chr15:73460913-73460935_HEK293_control_rep_1 836_E12_hg19_chr15:73460913-73460935_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535690 836_D12_hg19_chr12:93412649-93412671_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653559 836_D12_hg19_chr12:93412649-93412671_HEK293_control_rep_1 836_D12_hg19_chr12:93412649-93412671_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535691 833_F12_hg38_chr5:32505873-32505895_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653561 833_F12_hg38_chr5:32505873-32505895_HEK293_edited_rep_1 833_F12_hg38_chr5:32505873-32505895_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535692 833_G12_hg38_chr5:56876246-56876268_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653562 833_G12_hg38_chr5:56876246-56876268_HEK293_edited_rep_1 833_G12_hg38_chr5:56876246-56876268_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535693 833_H12_hg38_chr5:63176882-63176904_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653563 833_H12_hg38_chr5:63176882-63176904_HEK293_edited_rep_1 833_H12_hg38_chr5:63176882-63176904_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535694 834_A1_hg38_chr5:66329834-66329856_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653565 834_A1_hg38_chr5:66329834-66329856_HEK293_edited_rep_1 834_A1_hg38_chr5:66329834-66329856_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535695 834_B1_hg38_chr5:80832231-80832253_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653564 834_B1_hg38_chr5:80832231-80832253_HEK293_edited_rep_1 834_B1_hg38_chr5:80832231-80832253_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535696 834_C1_hg38_chr5:81087694-81087716_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653566 834_C1_hg38_chr5:81087694-81087716_HEK293_edited_rep_1 834_C1_hg38_chr5:81087694-81087716_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535697 834_D1_hg38_chr5:82664959-82664981_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653568 834_D1_hg38_chr5:82664959-82664981_HEK293_edited_rep_1 834_D1_hg38_chr5:82664959-82664981_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535698 834_E1_hg38_chr5:91300731-91300753_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653567 834_E1_hg38_chr5:91300731-91300753_HEK293_edited_rep_1 834_E1_hg38_chr5:91300731-91300753_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535699 839_H2_hg19_chr14:65417229-65417251_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653569 839_H2_hg19_chr14:65417229-65417251_HEK293_edited_rep_1 839_H2_hg19_chr14:65417229-65417251_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535700 835_A1_hg19_chr5:142433047-142433069_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653570 835_A1_hg19_chr5:142433047-142433069_HEK293_control_rep_1 835_A1_hg19_chr5:142433047-142433069_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535701 839_E12_hg19_chr15:73460913-73460935_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653573 839_E12_hg19_chr15:73460913-73460935_HEK293_edited_rep_1 839_E12_hg19_chr15:73460913-73460935_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535702 839_D12_hg19_chr12:93412649-93412671_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653571 839_D12_hg19_chr12:93412649-93412671_HEK293_edited_rep_1 839_D12_hg19_chr12:93412649-93412671_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535703 839_G12_hg19_chr11:121236195-121236217_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653575 839_G12_hg19_chr11:121236195-121236217_HEK293_edited_rep_1 839_G12_hg19_chr11:121236195-121236217_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535704 839_F12_hg19_chr1:35302483-35302505_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653572 839_F12_hg19_chr1:35302483-35302505_HEK293_edited_rep_1 839_F12_hg19_chr1:35302483-35302505_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535705 839_A12_hg19_chr1:4425560-4425582_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653574 839_A12_hg19_chr1:4425560-4425582_HEK293_edited_rep_1 839_A12_hg19_chr1:4425560-4425582_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535706 839_H11_hg19_chr8:144028903-144028925_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653576 839_H11_hg19_chr8:144028903-144028925_HEK293_edited_rep_1 839_H11_hg19_chr8:144028903-144028925_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535707 839_C12_hg19_chr17:29171904-29171926_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653577 839_C12_hg19_chr17:29171904-29171926_HEK293_edited_rep_1 839_C12_hg19_chr17:29171904-29171926_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535708 839_B12_hg19_chr7:73871682-73871704_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653579 839_B12_hg19_chr7:73871682-73871704_HEK293_edited_rep_1 839_B12_hg19_chr7:73871682-73871704_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535709 831_C5_hg38_chr16:66579941-66579963_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653578 831_C5_hg38_chr16:66579941-66579963_HEK293_edited_rep_1 831_C5_hg38_chr16:66579941-66579963_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535710 1734_B7_hg38_chr16:31473582-31473604_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653580 1734_B7_hg38_chr16:31473582-31473604_HEK293_control_rep_1 1734_B7_hg38_chr16:31473582-31473604_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535711 1734_G8_hg38_chr12:56612249-56612271_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653582 1734_G8_hg38_chr12:56612249-56612271_HEK293_control_rep_1 1734_G8_hg38_chr12:56612249-56612271_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535712 835_F6_hg19_chr16:67036555-67036577_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653581 835_F6_hg19_chr16:67036555-67036577_HEK293_control_rep_1 835_F6_hg19_chr16:67036555-67036577_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535713 835_G6_hg19_chr17:71928063-71928085_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653583 835_G6_hg19_chr17:71928063-71928085_HEK293_control_rep_1 835_G6_hg19_chr17:71928063-71928085_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535714 835_H6_hg19_chr1:2524160-2524182_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653585 835_H6_hg19_chr1:2524160-2524182_HEK293_control_rep_1 835_H6_hg19_chr1:2524160-2524182_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535715 835_A7_hg19_chr15:86309734-86309756_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653584 835_A7_hg19_chr15:86309734-86309756_HEK293_control_rep_1 835_A7_hg19_chr15:86309734-86309756_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535716 835_B6_hg19_chr6:36765464-36765486_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653587 835_B6_hg19_chr6:36765464-36765486_HEK293_control_rep_1 835_B6_hg19_chr6:36765464-36765486_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535717 835_C6_hg19_chr4:170332211-170332233_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653586 835_C6_hg19_chr4:170332211-170332233_HEK293_control_rep_1 835_C6_hg19_chr4:170332211-170332233_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535718 835_D6_hg19_chr11:118039016-118039038_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653588 835_D6_hg19_chr11:118039016-118039038_HEK293_control_rep_1 835_D6_hg19_chr11:118039016-118039038_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535719 835_E6_hg19_chrX:65856095-65856117_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653589 835_E6_hg19_chrX:65856095-65856117_HEK293_control_rep_1 835_E6_hg19_chrX:65856095-65856117_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535720 835_B7_hg19_chr22:36924541-36924563_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653590 835_B7_hg19_chr22:36924541-36924563_HEK293_control_rep_1 835_B7_hg19_chr22:36924541-36924563_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535721 835_C7_hg19_chr17:4423146-4423168_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653593 835_C7_hg19_chr17:4423146-4423168_HEK293_control_rep_1 835_C7_hg19_chr17:4423146-4423168_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535722 840_B10_hg19_chr1:7060175-7060197_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653591 840_B10_hg19_chr1:7060175-7060197_HEK293_edited_rep_1 840_B10_hg19_chr1:7060175-7060197_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535723 1734_E7_hg38_chr1:232661991-232662013_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653592 1734_E7_hg38_chr1:232661991-232662013_HEK293_control_rep_1 1734_E7_hg38_chr1:232661991-232662013_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535724 840_F10_hg19_chr1:245291115-245291137_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653595 840_F10_hg19_chr1:245291115-245291137_HEK293_edited_rep_1 840_F10_hg19_chr1:245291115-245291137_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535725 833_C1_hg38_chr22:23554465-23554487_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653596 833_C1_hg38_chr22:23554465-23554487_HEK293_edited_rep_1 833_C1_hg38_chr22:23554465-23554487_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535726 833_B1_hg38_chr22:21919532-21919554_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653594 833_B1_hg38_chr22:21919532-21919554_HEK293_edited_rep_1 833_B1_hg38_chr22:21919532-21919554_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535727 833_A1_hg38_chr22:20729333-20729355_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653599 833_A1_hg38_chr22:20729333-20729355_HEK293_edited_rep_1 833_A1_hg38_chr22:20729333-20729355_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535728 832_H12_hg38_chr22:19710934-19710956_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653598 832_H12_hg38_chr22:19710934-19710956_HEK293_edited_rep_1 832_H12_hg38_chr22:19710934-19710956_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535729 832_G12_hg38_chr22:17971840-17971862_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653601 832_G12_hg38_chr22:17971840-17971862_HEK293_edited_rep_1 832_G12_hg38_chr22:17971840-17971862_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535730 832_F12_hg38_chr22:17010845-17010867_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653603 832_F12_hg38_chr22:17010845-17010867_HEK293_edited_rep_1 832_F12_hg38_chr22:17010845-17010867_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535731 832_E12_hg38_chr21:46219338-46219360_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653597 832_E12_hg38_chr21:46219338-46219360_HEK293_edited_rep_1 832_E12_hg38_chr21:46219338-46219360_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535732 832_D12_hg38_chr21:44861622-44861644_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653600 832_D12_hg38_chr21:44861622-44861644_HEK293_edited_rep_1 832_D12_hg38_chr21:44861622-44861644_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535733 832_C12_hg38_chr21:42269762-42269784_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653604 832_C12_hg38_chr21:42269762-42269784_HEK293_edited_rep_1 832_C12_hg38_chr21:42269762-42269784_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535734 832_B12_hg38_chr21:37962177-37962199_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653602 832_B12_hg38_chr21:37962177-37962199_HEK293_edited_rep_1 832_B12_hg38_chr21:37962177-37962199_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535735 838_E2_hg19_chr12:50284997-50285019_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653606 838_E2_hg19_chr12:50284997-50285019_HEK293_edited_rep_1 838_E2_hg19_chr12:50284997-50285019_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535736 838_D2_hg19_chr1:9385356-9385378_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653607 838_D2_hg19_chr1:9385356-9385378_HEK293_edited_rep_1 838_D2_hg19_chr1:9385356-9385378_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535737 839_A7_hg19_chr4:185181290-185181312_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653605 839_A7_hg19_chr4:185181290-185181312_HEK293_edited_rep_1 839_A7_hg19_chr4:185181290-185181312_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535738 838_E1_hg19_chr3:10127644-10127666_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653608 838_E1_hg19_chr3:10127644-10127666_HEK293_edited_rep_1 838_E1_hg19_chr3:10127644-10127666_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535739 838_D1_hg19_chr11:120979641-120979663_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653609 838_D1_hg19_chr11:120979641-120979663_HEK293_edited_rep_1 838_D1_hg19_chr11:120979641-120979663_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535740 838_G1_hg19_chr13:106612912-106612934_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653614 838_G1_hg19_chr13:106612912-106612934_HEK293_edited_rep_1 838_G1_hg19_chr13:106612912-106612934_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535741 838_F1_hg19_chr2:111977953-111977975_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653610 838_F1_hg19_chr2:111977953-111977975_HEK293_edited_rep_1 838_F1_hg19_chr2:111977953-111977975_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535742 838_A2_hg19_chrX:23229670-23229692_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653611 838_A2_hg19_chrX:23229670-23229692_HEK293_edited_rep_1 838_A2_hg19_chrX:23229670-23229692_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535743 838_H1_hg19_chr20:60730921-60730943_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653613 838_H1_hg19_chr20:60730921-60730943_HEK293_edited_rep_1 838_H1_hg19_chr20:60730921-60730943_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535744 838_C2_hg19_chr9:34350221-34350243_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653616 838_C2_hg19_chr9:34350221-34350243_HEK293_edited_rep_1 838_C2_hg19_chr9:34350221-34350243_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535745 838_B2_hg19_chr8:141962476-141962498_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653612 838_B2_hg19_chr8:141962476-141962498_HEK293_edited_rep_1 838_B2_hg19_chr8:141962476-141962498_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535746 838_H8_hg19_chr13:111287204-111287226_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653617 838_H8_hg19_chr13:111287204-111287226_HEK293_edited_rep_1 838_H8_hg19_chr13:111287204-111287226_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535747 838_A9_hg19_chr9:39193157-39193179_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653615 838_A9_hg19_chr9:39193157-39193179_HEK293_edited_rep_1 838_A9_hg19_chr9:39193157-39193179_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535748 836_E6_hg19_chr5:858373-858395_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653618 836_E6_hg19_chr5:858373-858395_HEK293_control_rep_1 836_E6_hg19_chr5:858373-858395_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535749 836_D6_hg19_chr11:1949453-1949475_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653619 836_D6_hg19_chr11:1949453-1949475_HEK293_control_rep_1 836_D6_hg19_chr11:1949453-1949475_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535750 838_D9_hg19_chr4:1718259-1718281_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653621 838_D9_hg19_chr4:1718259-1718281_HEK293_edited_rep_1 838_D9_hg19_chr4:1718259-1718281_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535751 838_E9_hg19_chr12:132311857-132311879_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653620 838_E9_hg19_chr12:132311857-132311879_HEK293_edited_rep_1 838_E9_hg19_chr12:132311857-132311879_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535752 838_F9_hg19_chr16:33687030-33687052_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653622 838_F9_hg19_chr16:33687030-33687052_HEK293_edited_rep_1 838_F9_hg19_chr16:33687030-33687052_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535753 838_G9_hg19_chr2:28510827-28510849_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653623 838_G9_hg19_chr2:28510827-28510849_HEK293_edited_rep_1 838_G9_hg19_chr2:28510827-28510849_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535754 836_G5_hg19_chr17:45792239-45792261_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653626 836_G5_hg19_chr17:45792239-45792261_HEK293_control_rep_1 836_G5_hg19_chr17:45792239-45792261_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535755 836_F5_hg19_chr3:47057119-47057141_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653627 836_F5_hg19_chr3:47057119-47057141_HEK293_control_rep_1 836_F5_hg19_chr3:47057119-47057141_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535756 836_E5_hg19_chr10:21056679-21056701_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653629 836_E5_hg19_chr10:21056679-21056701_HEK293_control_rep_1 836_E5_hg19_chr10:21056679-21056701_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535757 836_D5_hg19_chr20:42338563-42338585_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653624 836_D5_hg19_chr20:42338563-42338585_HEK293_control_rep_1 836_D5_hg19_chr20:42338563-42338585_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535758 836_C6_hg19_chr1:181190077-181190099_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653625 836_C6_hg19_chr1:181190077-181190099_HEK293_control_rep_1 836_C6_hg19_chr1:181190077-181190099_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535759 836_B6_hg19_chr1:201367550-201367572_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653628 836_B6_hg19_chr1:201367550-201367572_HEK293_control_rep_1 836_B6_hg19_chr1:201367550-201367572_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535760 836_A6_hg19_chr9:124526198-124526220_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653630 836_A6_hg19_chr9:124526198-124526220_HEK293_control_rep_1 836_A6_hg19_chr9:124526198-124526220_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535761 836_H5_hg19_chr7:16099367-16099389_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653632 836_H5_hg19_chr7:16099367-16099389_HEK293_control_rep_1 836_H5_hg19_chr7:16099367-16099389_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535762 839_G7_hg19_chr5:53554410-53554432_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653633 839_G7_hg19_chr5:53554410-53554432_HEK293_edited_rep_1 839_G7_hg19_chr5:53554410-53554432_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535763 834_E5_hg38_chr6:40872445-40872467_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653631 834_E5_hg38_chr6:40872445-40872467_HEK293_edited_rep_1 834_E5_hg38_chr6:40872445-40872467_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535764 834_D5_hg38_chr6:39939332-39939354_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653634 834_D5_hg38_chr6:39939332-39939354_HEK293_edited_rep_1 834_D5_hg38_chr6:39939332-39939354_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535765 831_C9_hg38_chr17:74319332-74319354_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653635 831_C9_hg38_chr17:74319332-74319354_HEK293_edited_rep_1 831_C9_hg38_chr17:74319332-74319354_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535766 831_B9_hg38_chr17:73820207-73820229_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653637 831_B9_hg38_chr17:73820207-73820229_HEK293_edited_rep_1 831_B9_hg38_chr17:73820207-73820229_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535767 831_A10_hg38_chr17:82667693-82667715_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653640 831_A10_hg38_chr17:82667693-82667715_HEK293_edited_rep_1 831_A10_hg38_chr17:82667693-82667715_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535768 831_H9_hg38_chr17:82240531-82240553_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653636 831_H9_hg38_chr17:82240531-82240553_HEK293_edited_rep_1 831_H9_hg38_chr17:82240531-82240553_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535769 831_G9_hg38_chr17:76935169-76935191_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653641 831_G9_hg38_chr17:76935169-76935191_HEK293_edited_rep_1 831_G9_hg38_chr17:76935169-76935191_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535770 831_F9_hg38_chr17:75774778-75774800_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653643 831_F9_hg38_chr17:75774778-75774800_HEK293_edited_rep_1 831_F9_hg38_chr17:75774778-75774800_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535771 834_E4_hg38_chr6:10826226-10826248_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653638 834_E4_hg38_chr6:10826226-10826248_HEK293_edited_rep_1 834_E4_hg38_chr6:10826226-10826248_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535772 834_D4_hg38_chr6:6260792-6260814_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653645 834_D4_hg38_chr6:6260792-6260814_HEK293_edited_rep_1 834_D4_hg38_chr6:6260792-6260814_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535773 834_G4_hg38_chr6:15495312-15495334_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653639 834_G4_hg38_chr6:15495312-15495334_HEK293_edited_rep_1 834_G4_hg38_chr6:15495312-15495334_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535774 834_F4_hg38_chr6:14316136-14316158_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653642 834_F4_hg38_chr6:14316136-14316158_HEK293_edited_rep_1 834_F4_hg38_chr6:14316136-14316158_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535775 834_A5_hg38_chr6:21360401-21360423_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653644 834_A5_hg38_chr6:21360401-21360423_HEK293_edited_rep_1 834_A5_hg38_chr6:21360401-21360423_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535776 834_H4_hg38_chr6:17938404-17938426_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653646 834_H4_hg38_chr6:17938404-17938426_HEK293_edited_rep_1 834_H4_hg38_chr6:17938404-17938426_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535777 834_C5_hg38_chr6:36775364-36775386_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653647 834_C5_hg38_chr6:36775364-36775386_HEK293_edited_rep_1 834_C5_hg38_chr6:36775364-36775386_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535778 834_B5_hg38_chr6:33878896-33878918_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653650 834_B5_hg38_chr6:33878896-33878918_HEK293_edited_rep_1 834_B5_hg38_chr6:33878896-33878918_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535779 837_F11_hg19_chr1:157075975-157075997_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653648 837_F11_hg19_chr1:157075975-157075997_HEK293_control_rep_1 837_F11_hg19_chr1:157075975-157075997_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535780 833_B4_hg38_chr22:49685841-49685863_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653649 833_B4_hg38_chr22:49685841-49685863_HEK293_edited_rep_1 833_B4_hg38_chr22:49685841-49685863_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535781 833_C4_hg38_chr22:50327580-50327602_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653653 833_C4_hg38_chr22:50327580-50327602_HEK293_edited_rep_1 833_C4_hg38_chr22:50327580-50327602_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535782 833_H3_hg38_chr22:48555297-48555319_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653652 833_H3_hg38_chr22:48555297-48555319_HEK293_edited_rep_1 833_H3_hg38_chr22:48555297-48555319_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535783 833_A4_hg38_chr22:49304571-49304593_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653657 833_A4_hg38_chr22:49304571-49304593_HEK293_edited_rep_1 833_A4_hg38_chr22:49304571-49304593_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535784 833_F4_hg38_chr3:11492632-11492654_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653654 833_F4_hg38_chr3:11492632-11492654_HEK293_edited_rep_1 833_F4_hg38_chr3:11492632-11492654_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535785 833_G4_hg38_chr3:13391700-13391722_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653655 833_G4_hg38_chr3:13391700-13391722_HEK293_edited_rep_1 833_G4_hg38_chr3:13391700-13391722_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535786 833_D4_hg38_chr22:50673761-50673783_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653651 833_D4_hg38_chr22:50673761-50673783_HEK293_edited_rep_1 833_D4_hg38_chr22:50673761-50673783_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535787 833_E4_hg38_chr3:2312528-2312550_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653656 833_E4_hg38_chr3:2312528-2312550_HEK293_edited_rep_1 833_E4_hg38_chr3:2312528-2312550_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535788 837_C11_hg19_chr8:143220958-143220980_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653659 837_C11_hg19_chr8:143220958-143220980_HEK293_control_rep_1 837_C11_hg19_chr8:143220958-143220980_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535789 833_H4_hg38_chr3:13538653-13538675_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653660 833_H4_hg38_chr3:13538653-13538675_HEK293_edited_rep_1 833_H4_hg38_chr3:13538653-13538675_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535790 833_A5_hg38_chr3:17896136-17896158_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653658 833_A5_hg38_chr3:17896136-17896158_HEK293_edited_rep_1 833_A5_hg38_chr3:17896136-17896158_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535791 1734_F7_hg38_chr2:109398242-109398264_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653665 1734_F7_hg38_chr2:109398242-109398264_HEK293_control_rep_1 1734_F7_hg38_chr2:109398242-109398264_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535792 837_B11_hg19_chr16:2900150-2900172_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653661 837_B11_hg19_chr16:2900150-2900172_HEK293_control_rep_1 837_B11_hg19_chr16:2900150-2900172_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535793 839_E6_hg19_chr5:858373-858395_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653662 839_E6_hg19_chr5:858373-858395_HEK293_edited_rep_1 839_E6_hg19_chr5:858373-858395_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535794 839_D6_hg19_chr11:1949453-1949475_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653664 839_D6_hg19_chr11:1949453-1949475_HEK293_edited_rep_1 839_D6_hg19_chr11:1949453-1949475_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535795 839_C6_hg19_chr1:181190077-181190099_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653663 839_C6_hg19_chr1:181190077-181190099_HEK293_edited_rep_1 839_C6_hg19_chr1:181190077-181190099_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535796 839_B6_hg19_chr1:201367550-201367572_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653666 839_B6_hg19_chr1:201367550-201367572_HEK293_edited_rep_1 839_B6_hg19_chr1:201367550-201367572_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535797 839_A6_hg19_chr9:124526198-124526220_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653668 839_A6_hg19_chr9:124526198-124526220_HEK293_edited_rep_1 839_A6_hg19_chr9:124526198-124526220_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535798 839_H5_hg19_chr7:16099367-16099389_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653671 839_H5_hg19_chr7:16099367-16099389_HEK293_edited_rep_1 839_H5_hg19_chr7:16099367-16099389_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535799 839_G5_hg19_chr17:45792239-45792261_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653667 839_G5_hg19_chr17:45792239-45792261_HEK293_edited_rep_1 839_G5_hg19_chr17:45792239-45792261_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535800 839_F5_hg19_chr3:47057119-47057141_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653673 839_F5_hg19_chr3:47057119-47057141_HEK293_edited_rep_1 839_F5_hg19_chr3:47057119-47057141_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535801 837_H10_hg19_chr1:18451047-18451069_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653669 837_H10_hg19_chr1:18451047-18451069_HEK293_control_rep_1 837_H10_hg19_chr1:18451047-18451069_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535802 839_G6_hg19_chr8:11553531-11553553_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653670 839_G6_hg19_chr8:11553531-11553553_HEK293_edited_rep_1 839_G6_hg19_chr8:11553531-11553553_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535803 839_F6_hg19_chr9:123505399-123505421_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653672 839_F6_hg19_chr9:123505399-123505421_HEK293_edited_rep_1 839_F6_hg19_chr9:123505399-123505421_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535804 836_G11_hg19_chr2:8357445-8357467_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653674 836_G11_hg19_chr2:8357445-8357467_HEK293_control_rep_1 836_G11_hg19_chr2:8357445-8357467_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535805 840_G10_hg19_chr1:30953115-30953137_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653675 840_G10_hg19_chr1:30953115-30953137_HEK293_edited_rep_1 840_G10_hg19_chr1:30953115-30953137_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535806 840_F11_hg19_chr1:157075975-157075997_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653678 840_F11_hg19_chr1:157075975-157075997_HEK293_edited_rep_1 840_F11_hg19_chr1:157075975-157075997_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535807 836_F11_hg19_chr6:158387927-158387949_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653680 836_F11_hg19_chr6:158387927-158387949_HEK293_control_rep_1 836_F11_hg19_chr6:158387927-158387949_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535808 837_C5_hg19_chr6:14796008-14796030_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653677 837_C5_hg19_chr6:14796008-14796030_HEK293_control_rep_1 837_C5_hg19_chr6:14796008-14796030_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535809 837_B5_hg19_chr6:53200088-53200110_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653681 837_B5_hg19_chr6:53200088-53200110_HEK293_control_rep_1 837_B5_hg19_chr6:53200088-53200110_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535810 837_E5_hg19_chr2:73338879-73338901_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653679 837_E5_hg19_chr2:73338879-73338901_HEK293_control_rep_1 837_E5_hg19_chr2:73338879-73338901_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535811 837_D5_hg19_chr19:51952701-51952723_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653684 837_D5_hg19_chr19:51952701-51952723_HEK293_control_rep_1 837_D5_hg19_chr19:51952701-51952723_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535812 833_F8_hg38_chr3:124991976-124991998_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653686 833_F8_hg38_chr3:124991976-124991998_HEK293_edited_rep_1 833_F8_hg38_chr3:124991976-124991998_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535813 833_G8_hg38_chr3:127444039-127444061_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653682 833_G8_hg38_chr3:127444039-127444061_HEK293_edited_rep_1 833_G8_hg38_chr3:127444039-127444061_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535814 837_A5_hg19_chr7:378982-379004_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653683 837_A5_hg19_chr7:378982-379004_HEK293_control_rep_1 837_A5_hg19_chr7:378982-379004_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535815 837_H4_hg19_chr16:89774898-89774920_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653685 837_H4_hg19_chr16:89774898-89774920_HEK293_control_rep_1 837_H4_hg19_chr16:89774898-89774920_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535816 833_B8_hg38_chr3:95821314-95821336_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653688 833_B8_hg38_chr3:95821314-95821336_HEK293_edited_rep_1 833_B8_hg38_chr3:95821314-95821336_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535817 833_C8_hg38_chr3:97591805-97591827_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653687 833_C8_hg38_chr3:97591805-97591827_HEK293_edited_rep_1 833_C8_hg38_chr3:97591805-97591827_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535818 833_D8_hg38_chr3:119582232-119582254_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653689 833_D8_hg38_chr3:119582232-119582254_HEK293_edited_rep_1 833_D8_hg38_chr3:119582232-119582254_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535819 833_E8_hg38_chr3:123475106-123475128_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653695 833_E8_hg38_chr3:123475106-123475128_HEK293_edited_rep_1 833_E8_hg38_chr3:123475106-123475128_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535820 833_F7_hg38_chr3:69359253-69359275_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653697 833_F7_hg38_chr3:69359253-69359275_HEK293_edited_rep_1 833_F7_hg38_chr3:69359253-69359275_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535821 833_G7_hg38_chr3:72690894-72690916_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653693 833_G7_hg38_chr3:72690894-72690916_HEK293_edited_rep_1 833_G7_hg38_chr3:72690894-72690916_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535822 833_H7_hg38_chr3:72973390-72973412_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653701 833_H7_hg38_chr3:72973390-72973412_HEK293_edited_rep_1 833_H7_hg38_chr3:72973390-72973412_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535823 833_A8_hg38_chr3:87069500-87069522_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653696 833_A8_hg38_chr3:87069500-87069522_HEK293_edited_rep_1 833_A8_hg38_chr3:87069500-87069522_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535824 834_E11_hg38_chr7:117659140-117659162_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653698 834_E11_hg38_chr7:117659140-117659162_HEK293_edited_rep_1 834_E11_hg38_chr7:117659140-117659162_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535825 834_D11_hg38_chr7:116231162-116231184_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653690 834_D11_hg38_chr7:116231162-116231184_HEK293_edited_rep_1 834_D11_hg38_chr7:116231162-116231184_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535826 834_C11_hg38_chr7:111499142-111499164_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653692 834_C11_hg38_chr7:111499142-111499164_HEK293_edited_rep_1 834_C11_hg38_chr7:111499142-111499164_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535827 834_B11_hg38_chr7:100132891-100132913_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653691 834_B11_hg38_chr7:100132891-100132913_HEK293_edited_rep_1 834_B11_hg38_chr7:100132891-100132913_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535828 834_A11_hg38_chr7:99696803-99696825_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653699 834_A11_hg38_chr7:99696803-99696825_HEK293_edited_rep_1 834_A11_hg38_chr7:99696803-99696825_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535829 834_H10_hg38_chr7:98374465-98374487_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653700 834_H10_hg38_chr7:98374465-98374487_HEK293_edited_rep_1 834_H10_hg38_chr7:98374465-98374487_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535830 834_G10_hg38_chr7:93890555-93890577_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653702 834_G10_hg38_chr7:93890555-93890577_HEK293_edited_rep_1 834_G10_hg38_chr7:93890555-93890577_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535831 834_F10_hg38_chr7:86364187-86364209_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653694 834_F10_hg38_chr7:86364187-86364209_HEK293_edited_rep_1 834_F10_hg38_chr7:86364187-86364209_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535832 834_G11_hg38_chr7:130368297-130368319_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653703 834_G11_hg38_chr7:130368297-130368319_HEK293_edited_rep_1 834_G11_hg38_chr7:130368297-130368319_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535833 834_F11_hg38_chr7:120641561-120641583_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653704 834_F11_hg38_chr7:120641561-120641583_HEK293_edited_rep_1 834_F11_hg38_chr7:120641561-120641583_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535834 840_A5_hg19_chr7:378982-379004_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653705 840_A5_hg19_chr7:378982-379004_HEK293_edited_rep_1 840_A5_hg19_chr7:378982-379004_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535835 836_G12_hg19_chr11:121236195-121236217_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653706 836_G12_hg19_chr11:121236195-121236217_HEK293_control_rep_1 836_G12_hg19_chr11:121236195-121236217_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535836 840_C5_hg19_chr6:14796008-14796030_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653708 840_C5_hg19_chr6:14796008-14796030_HEK293_edited_rep_1 840_C5_hg19_chr6:14796008-14796030_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535837 840_B5_hg19_chr6:53200088-53200110_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653707 840_B5_hg19_chr6:53200088-53200110_HEK293_edited_rep_1 840_B5_hg19_chr6:53200088-53200110_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535838 840_E5_hg19_chr2:73338879-73338901_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653711 840_E5_hg19_chr2:73338879-73338901_HEK293_edited_rep_1 840_E5_hg19_chr2:73338879-73338901_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535839 840_D5_hg19_chr19:51952701-51952723_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653709 840_D5_hg19_chr19:51952701-51952723_HEK293_edited_rep_1 840_D5_hg19_chr19:51952701-51952723_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535840 840_G5_hg19_chr11:117099065-117099087_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653712 840_G5_hg19_chr11:117099065-117099087_HEK293_edited_rep_1 840_G5_hg19_chr11:117099065-117099087_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535841 836_F12_hg19_chr1:35302483-35302505_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653710 836_F12_hg19_chr1:35302483-35302505_HEK293_control_rep_1 836_F12_hg19_chr1:35302483-35302505_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535842 840_A6_hg19_chr1:95200617-95200639_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653713 840_A6_hg19_chr1:95200617-95200639_HEK293_edited_rep_1 840_A6_hg19_chr1:95200617-95200639_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535843 840_H5_hg19_chr1:51754769-51754791_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653714 840_H5_hg19_chr1:51754769-51754791_HEK293_edited_rep_1 840_H5_hg19_chr1:51754769-51754791_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535844 1734_F5_hg38_chr5:170084409-170084431_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653716 1734_F5_hg38_chr5:170084409-170084431_HEK293_control_rep_1 1734_F5_hg38_chr5:170084409-170084431_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535845 1734_G5_hg38_chr13:111414262-111414284_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653715 1734_G5_hg38_chr13:111414262-111414284_HEK293_control_rep_1 1734_G5_hg38_chr13:111414262-111414284_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535846 1734_H5_hg38_chr12:111626791-111626813_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653718 1734_H5_hg38_chr12:111626791-111626813_HEK293_control_rep_1 1734_H5_hg38_chr12:111626791-111626813_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535847 1734_A6_hg38_chr19:49658566-49658588_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653717 1734_A6_hg38_chr19:49658566-49658588_HEK293_control_rep_1 1734_A6_hg38_chr19:49658566-49658588_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535848 1734_B6_hg38_chr9:128495873-128495895_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653719 1734_B6_hg38_chr9:128495873-128495895_HEK293_control_rep_1 1734_B6_hg38_chr9:128495873-128495895_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535849 837_C6_hg19_chr15:44482442-44482464_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653720 837_C6_hg19_chr15:44482442-44482464_HEK293_control_rep_1 837_C6_hg19_chr15:44482442-44482464_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535850 838_G8_hg19_chr1:5708155-5708177_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653722 838_G8_hg19_chr1:5708155-5708177_HEK293_edited_rep_1 838_G8_hg19_chr1:5708155-5708177_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535851 838_F8_hg19_chr15:65693902-65693924_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653721 838_F8_hg19_chr15:65693902-65693924_HEK293_edited_rep_1 838_F8_hg19_chr15:65693902-65693924_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535852 836_D11_hg19_chr6:34439724-34439746_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653724 836_D11_hg19_chr6:34439724-34439746_HEK293_control_rep_1 836_D11_hg19_chr6:34439724-34439746_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535853 836_E11_hg19_chr22:22874988-22875010_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653723 836_E11_hg19_chr22:22874988-22875010_HEK293_control_rep_1 836_E11_hg19_chr22:22874988-22875010_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535854 836_D10_hg19_chr10:120117150-120117172_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653725 836_D10_hg19_chr10:120117150-120117172_HEK293_control_rep_1 836_D10_hg19_chr10:120117150-120117172_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535855 836_E10_hg19_chr20:44972632-44972654_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653726 836_E10_hg19_chr20:44972632-44972654_HEK293_control_rep_1 836_E10_hg19_chr20:44972632-44972654_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535856 836_F10_hg19_chr3:63898822-63898844_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653727 836_F10_hg19_chr3:63898822-63898844_HEK293_control_rep_1 836_F10_hg19_chr3:63898822-63898844_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535857 836_G10_hg19_chr2:112173057-112173079_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653728 836_G10_hg19_chr2:112173057-112173079_HEK293_control_rep_1 836_G10_hg19_chr2:112173057-112173079_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535858 836_H10_hg19_chr22:37508524-37508546_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653729 836_H10_hg19_chr22:37508524-37508546_HEK293_control_rep_1 836_H10_hg19_chr22:37508524-37508546_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535859 836_A11_hg19_chr16:49848326-49848348_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653730 836_A11_hg19_chr16:49848326-49848348_HEK293_control_rep_1 836_A11_hg19_chr16:49848326-49848348_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535860 836_B11_hg19_chr2:16151657-16151679_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653731 836_B11_hg19_chr2:16151657-16151679_HEK293_control_rep_1 836_B11_hg19_chr2:16151657-16151679_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535861 836_C11_hg19_chr17:70285215-70285237_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653732 836_C11_hg19_chr17:70285215-70285237_HEK293_control_rep_1 836_C11_hg19_chr17:70285215-70285237_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535862 840_B2_hg19_chr12:112064595-112064617_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653736 840_B2_hg19_chr12:112064595-112064617_HEK293_edited_rep_1 840_B2_hg19_chr12:112064595-112064617_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535863 840_A1_hg19_chr16:32980690-32980712_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653733 840_A1_hg19_chr16:32980690-32980712_HEK293_edited_rep_1 840_A1_hg19_chr16:32980690-32980712_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535864 839_H12_hg19_chr16:32037129-32037151_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653734 839_H12_hg19_chr16:32037129-32037151_HEK293_edited_rep_1 839_H12_hg19_chr16:32037129-32037151_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535865 832_E7_hg38_chr2:204272731-204272753_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653735 832_E7_hg38_chr2:204272731-204272753_HEK293_edited_rep_1 832_E7_hg38_chr2:204272731-204272753_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535866 832_D7_hg38_chr2:186975417-186975439_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653737 832_D7_hg38_chr2:186975417-186975439_HEK293_edited_rep_1 832_D7_hg38_chr2:186975417-186975439_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535867 832_C7_hg38_chr2:156905178-156905200_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653738 832_C7_hg38_chr2:156905178-156905200_HEK293_edited_rep_1 832_C7_hg38_chr2:156905178-156905200_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535868 832_B7_hg38_chr2:156399975-156399997_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653739 832_B7_hg38_chr2:156399975-156399997_HEK293_edited_rep_1 832_B7_hg38_chr2:156399975-156399997_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535869 832_A8_hg38_chr2:217281399-217281421_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653740 832_A8_hg38_chr2:217281399-217281421_HEK293_edited_rep_1 832_A8_hg38_chr2:217281399-217281421_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535870 832_H7_hg38_chr2:216535448-216535470_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653742 832_H7_hg38_chr2:216535448-216535470_HEK293_edited_rep_1 832_H7_hg38_chr2:216535448-216535470_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535871 832_G7_hg38_chr2:208572858-208572880_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653744 832_G7_hg38_chr2:208572858-208572880_HEK293_edited_rep_1 832_G7_hg38_chr2:208572858-208572880_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535872 832_F7_hg38_chr2:204560661-204560683_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653741 832_F7_hg38_chr2:204560661-204560683_HEK293_edited_rep_1 832_F7_hg38_chr2:204560661-204560683_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535873 832_C8_hg38_chr2:224957140-224957162_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653743 832_C8_hg38_chr2:224957140-224957162_HEK293_edited_rep_1 832_C8_hg38_chr2:224957140-224957162_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535874 832_B8_hg38_chr2:222839707-222839729_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653745 832_B8_hg38_chr2:222839707-222839729_HEK293_edited_rep_1 832_B8_hg38_chr2:222839707-222839729_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535875 839_D2_hg19_chr6:33737867-33737889_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653746 839_D2_hg19_chr6:33737867-33737889_HEK293_edited_rep_1 839_D2_hg19_chr6:33737867-33737889_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535876 839_E2_hg19_chrX:48370088-48370110_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653751 839_E2_hg19_chrX:48370088-48370110_HEK293_edited_rep_1 839_E2_hg19_chrX:48370088-48370110_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535877 839_F2_hg19_chr6:41739322-41739344_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653749 839_F2_hg19_chr6:41739322-41739344_HEK293_edited_rep_1 839_F2_hg19_chr6:41739322-41739344_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535878 839_G2_hg19_chr19:34111061-34111083_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653747 839_G2_hg19_chr19:34111061-34111083_HEK293_edited_rep_1 839_G2_hg19_chr19:34111061-34111083_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535879 839_H1_hg19_chr6:37592409-37592431_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653750 839_H1_hg19_chr6:37592409-37592431_HEK293_edited_rep_1 839_H1_hg19_chr6:37592409-37592431_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535880 839_A2_hg19_chr10:72079191-72079213_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653748 839_A2_hg19_chr10:72079191-72079213_HEK293_edited_rep_1 839_A2_hg19_chr10:72079191-72079213_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535881 836_E1_hg19_chr9:131258152-131258174_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653753 836_E1_hg19_chr9:131258152-131258174_HEK293_control_rep_1 836_E1_hg19_chr9:131258152-131258174_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535882 836_D1_hg19_chr1:231074350-231074372_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653752 836_D1_hg19_chr1:231074350-231074372_HEK293_control_rep_1 836_D1_hg19_chr1:231074350-231074372_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535883 836_C1_hg19_chr3:38639828-38639850_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653754 836_C1_hg19_chr3:38639828-38639850_HEK293_control_rep_1 836_C1_hg19_chr3:38639828-38639850_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535884 836_B1_hg19_chr2:26762118-26762140_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653755 836_B1_hg19_chr2:26762118-26762140_HEK293_control_rep_1 836_B1_hg19_chr2:26762118-26762140_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535885 836_A1_hg19_chr21:45401548-45401570_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653756 836_A1_hg19_chr21:45401548-45401570_HEK293_control_rep_1 836_A1_hg19_chr21:45401548-45401570_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535886 835_H12_hg19_chr10:121266568-121266590_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653757 835_H12_hg19_chr10:121266568-121266590_HEK293_control_rep_1 835_H12_hg19_chr10:121266568-121266590_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535887 835_G12_hg19_chr4:183758607-183758629_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653758 835_G12_hg19_chr4:183758607-183758629_HEK293_control_rep_1 835_G12_hg19_chr4:183758607-183758629_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535888 835_F12_hg19_chr10:69247872-69247894_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653759 835_F12_hg19_chr10:69247872-69247894_HEK293_control_rep_1 835_F12_hg19_chr10:69247872-69247894_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535889 835_E12_hg19_chr8:144749069-144749091_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653760 835_E12_hg19_chr8:144749069-144749091_HEK293_control_rep_1 835_E12_hg19_chr8:144749069-144749091_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535890 835_D12_hg19_chr14:90989236-90989258_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653762 835_D12_hg19_chr14:90989236-90989258_HEK293_control_rep_1 835_D12_hg19_chr14:90989236-90989258_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535891 834_H11_hg38_chr7:130987548-130987570_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653761 834_H11_hg38_chr7:130987548-130987570_HEK293_edited_rep_1 834_H11_hg38_chr7:130987548-130987570_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535892 834_A12_hg38_chr7:131558089-131558111_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653763 834_A12_hg38_chr7:131558089-131558111_HEK293_edited_rep_1 834_A12_hg38_chr7:131558089-131558111_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535893 835_D5_hg19_chr12:42790960-42790982_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653770 835_D5_hg19_chr12:42790960-42790982_HEK293_control_rep_1 835_D5_hg19_chr12:42790960-42790982_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535894 835_E5_hg19_chr8:10472670-10472692_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653768 835_E5_hg19_chr8:10472670-10472692_HEK293_control_rep_1 835_E5_hg19_chr8:10472670-10472692_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535895 835_F5_hg19_chr15:89175080-89175102_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653764 835_F5_hg19_chr15:89175080-89175102_HEK293_control_rep_1 835_F5_hg19_chr15:89175080-89175102_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535896 835_G5_hg19_chr12:57006033-57006055_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653769 835_G5_hg19_chr12:57006033-57006055_HEK293_control_rep_1 835_G5_hg19_chr12:57006033-57006055_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535897 835_H4_hg19_chr16:68431546-68431568_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653765 835_H4_hg19_chr16:68431546-68431568_HEK293_control_rep_1 835_H4_hg19_chr16:68431546-68431568_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535898 835_A5_hg19_chr17:73608985-73609007_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653767 835_A5_hg19_chr17:73608985-73609007_HEK293_control_rep_1 835_A5_hg19_chr17:73608985-73609007_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535899 835_B5_hg19_chr6:159181238-159181260_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653766 835_B5_hg19_chr6:159181238-159181260_HEK293_control_rep_1 835_B5_hg19_chr6:159181238-159181260_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535900 835_C5_hg19_chr10:87923063-87923085_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653771 835_C5_hg19_chr10:87923063-87923085_HEK293_control_rep_1 835_C5_hg19_chr10:87923063-87923085_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535901 839_C2_hg19_chr1:7448860-7448882_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653774 839_C2_hg19_chr1:7448860-7448882_HEK293_edited_rep_1 839_C2_hg19_chr1:7448860-7448882_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535902 835_H5_hg19_chr10:49999950-49999972_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653772 835_H5_hg19_chr10:49999950-49999972_HEK293_control_rep_1 835_H5_hg19_chr10:49999950-49999972_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535903 835_A6_hg19_chr11:77033596-77033618_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653773 835_A6_hg19_chr11:77033596-77033618_HEK293_control_rep_1 835_A6_hg19_chr11:77033596-77033618_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535904 832_F11_hg38_chr21:15494514-15494536_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653778 832_F11_hg38_chr21:15494514-15494536_HEK293_edited_rep_1 832_F11_hg38_chr21:15494514-15494536_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535905 832_G11_hg38_chr21:25383884-25383906_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653946 832_G11_hg38_chr21:25383884-25383906_HEK293_edited_rep_1 832_G11_hg38_chr21:25383884-25383906_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535906 832_D11_hg38_chr20:63236886-63236908_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653780 832_D11_hg38_chr20:63236886-63236908_HEK293_edited_rep_1 832_D11_hg38_chr20:63236886-63236908_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535907 832_E11_hg38_chr20:63931357-63931379_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653775 832_E11_hg38_chr20:63931357-63931379_HEK293_edited_rep_1 832_E11_hg38_chr20:63931357-63931379_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535908 832_B11_hg38_chr20:62283954-62283976_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653777 832_B11_hg38_chr20:62283954-62283976_HEK293_edited_rep_1 832_B11_hg38_chr20:62283954-62283976_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535909 832_C11_hg38_chr20:63016448-63016470_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653948 832_C11_hg38_chr20:63016448-63016470_HEK293_edited_rep_1 832_C11_hg38_chr20:63016448-63016470_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535910 832_H10_hg38_chr20:57844638-57844660_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653781 832_H10_hg38_chr20:57844638-57844660_HEK293_edited_rep_1 832_H10_hg38_chr20:57844638-57844660_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535911 832_A11_hg38_chr20:62208203-62208225_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653776 832_A11_hg38_chr20:62208203-62208225_HEK293_edited_rep_1 832_A11_hg38_chr20:62208203-62208225_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535912 1734_G6_hg38_chr20:32510607-32510629_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653779 1734_G6_hg38_chr20:32510607-32510629_HEK293_control_rep_1 1734_G6_hg38_chr20:32510607-32510629_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535913 1734_F6_hg38_chr6:165770950-165770972_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653782 1734_F6_hg38_chr6:165770950-165770972_HEK293_control_rep_1 1734_F6_hg38_chr6:165770950-165770972_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535914 1734_A7_hg38_chr17:40352108-40352130_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653783 1734_A7_hg38_chr17:40352108-40352130_HEK293_control_rep_1 1734_A7_hg38_chr17:40352108-40352130_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535915 1734_H6_hg38_chr2:42973889-42973911_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653784 1734_H6_hg38_chr2:42973889-42973911_HEK293_control_rep_1 1734_H6_hg38_chr2:42973889-42973911_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535916 1734_C7_hg38_chr2:45296472-45296494_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653785 1734_C7_hg38_chr2:45296472-45296494_HEK293_control_rep_1 1734_C7_hg38_chr2:45296472-45296494_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535917 838_A7_hg19_chr15:86309734-86309756_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653788 838_A7_hg19_chr15:86309734-86309756_HEK293_edited_rep_1 838_A7_hg19_chr15:86309734-86309756_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535918 832_H11_hg38_chr21:31779218-31779240_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653786 832_H11_hg38_chr21:31779218-31779240_HEK293_edited_rep_1 832_H11_hg38_chr21:31779218-31779240_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535919 832_A12_hg38_chr21:35744355-35744377_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653787 832_A12_hg38_chr21:35744355-35744377_HEK293_edited_rep_1 832_A12_hg38_chr21:35744355-35744377_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535920 837_F9_hg19_chr13:107769207-107769229_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653789 837_F9_hg19_chr13:107769207-107769229_HEK293_control_rep_1 837_F9_hg19_chr13:107769207-107769229_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535921 837_G9_hg19_chr10:121198681-121198703_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653790 837_G9_hg19_chr10:121198681-121198703_HEK293_control_rep_1 837_G9_hg19_chr10:121198681-121198703_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535922 837_H9_hg19_chr17:5704930-5704952_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653792 837_H9_hg19_chr17:5704930-5704952_HEK293_control_rep_1 837_H9_hg19_chr17:5704930-5704952_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535923 837_A10_hg19_chr2:110014698-110014720_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653791 837_A10_hg19_chr2:110014698-110014720_HEK293_control_rep_1 837_A10_hg19_chr2:110014698-110014720_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535924 837_B10_hg19_chr1:7060175-7060197_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653793 837_B10_hg19_chr1:7060175-7060197_HEK293_control_rep_1 837_B10_hg19_chr1:7060175-7060197_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535925 837_C10_hg19_chr13:31618284-31618306_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653794 837_C10_hg19_chr13:31618284-31618306_HEK293_control_rep_1 837_C10_hg19_chr13:31618284-31618306_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535926 837_D10_hg19_chr15:29414767-29414789_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653796 837_D10_hg19_chr15:29414767-29414789_HEK293_control_rep_1 837_D10_hg19_chr15:29414767-29414789_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535927 837_E10_hg19_chr1:232797737-232797759_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653795 837_E10_hg19_chr1:232797737-232797759_HEK293_control_rep_1 837_E10_hg19_chr1:232797737-232797759_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535928 837_F10_hg19_chr1:245291115-245291137_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653797 837_F10_hg19_chr1:245291115-245291137_HEK293_control_rep_1 837_F10_hg19_chr1:245291115-245291137_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535929 837_G10_hg19_chr1:30953115-30953137_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653798 837_G10_hg19_chr1:30953115-30953137_HEK293_control_rep_1 837_G10_hg19_chr1:30953115-30953137_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535930 839_A3_hg19_chr3:186559608-186559630_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653799 839_A3_hg19_chr3:186559608-186559630_HEK293_edited_rep_1 839_A3_hg19_chr3:186559608-186559630_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535931 1734_G2_hg38_chr1:21601988-21602010_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653800 1734_G2_hg38_chr1:21601988-21602010_HEK293_control_rep_1 1734_G2_hg38_chr1:21601988-21602010_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535932 838_H6_hg19_chr1:2524160-2524182_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653801 838_H6_hg19_chr1:2524160-2524182_HEK293_edited_rep_1 838_H6_hg19_chr1:2524160-2524182_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535933 1734_F2_hg38_chr19:51368596-51368618_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653803 1734_F2_hg38_chr19:51368596-51368618_HEK293_control_rep_1 1734_F2_hg38_chr19:51368596-51368618_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535934 832_D4_hg38_chr2:71612330-71612352_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653802 832_D4_hg38_chr2:71612330-71612352_HEK293_edited_rep_1 832_D4_hg38_chr2:71612330-71612352_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535935 832_E4_hg38_chr2:73213515-73213537_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653804 832_E4_hg38_chr2:73213515-73213537_HEK293_edited_rep_1 832_E4_hg38_chr2:73213515-73213537_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535936 832_D3_hg38_chr2:29703895-29703917_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653805 832_D3_hg38_chr2:29703895-29703917_HEK293_edited_rep_1 832_D3_hg38_chr2:29703895-29703917_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535937 832_E3_hg38_chr2:45009904-45009926_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653807 832_E3_hg38_chr2:45009904-45009926_HEK293_edited_rep_1 832_E3_hg38_chr2:45009904-45009926_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535938 832_F3_hg38_chr2:45692820-45692842_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653806 832_F3_hg38_chr2:45692820-45692842_HEK293_edited_rep_1 832_F3_hg38_chr2:45692820-45692842_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535939 832_G3_hg38_chr2:55128458-55128480_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653808 832_G3_hg38_chr2:55128458-55128480_HEK293_edited_rep_1 832_G3_hg38_chr2:55128458-55128480_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535940 832_H3_hg38_chr2:56295783-56295805_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653810 832_H3_hg38_chr2:56295783-56295805_HEK293_edited_rep_1 832_H3_hg38_chr2:56295783-56295805_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535941 840_E3_hg19_chr5:857945-857967_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653809 840_E3_hg19_chr5:857945-857967_HEK293_edited_rep_1 840_E3_hg19_chr5:857945-857967_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535942 832_B4_hg38_chr2:69952971-69952993_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653811 832_B4_hg38_chr2:69952971-69952993_HEK293_edited_rep_1 832_B4_hg38_chr2:69952971-69952993_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535943 832_C4_hg38_chr2:70862740-70862762_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653813 832_C4_hg38_chr2:70862740-70862762_HEK293_edited_rep_1 832_C4_hg38_chr2:70862740-70862762_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535944 834_G6_hg38_chr6:97122976-97122998_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653815 834_G6_hg38_chr6:97122976-97122998_HEK293_edited_rep_1 834_G6_hg38_chr6:97122976-97122998_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535945 834_F6_hg38_chr6:79431850-79431872_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653814 834_F6_hg38_chr6:79431850-79431872_HEK293_edited_rep_1 834_F6_hg38_chr6:79431850-79431872_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535946 834_A6_hg38_chr6:45586313-45586335_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653812 834_A6_hg38_chr6:45586313-45586335_HEK293_edited_rep_1 834_A6_hg38_chr6:45586313-45586335_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535947 834_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653817 834_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 834_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535948 834_G5_hg38_chr6:43060411-43060433_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653818 834_G5_hg38_chr6:43060411-43060433_HEK293_edited_rep_1 834_G5_hg38_chr6:43060411-43060433_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535949 834_F5_hg38_chr6:40925705-40925727_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653816 834_F5_hg38_chr6:40925705-40925727_HEK293_edited_rep_1 834_F5_hg38_chr6:40925705-40925727_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535950 834_E6_hg38_chr6:78745244-78745266_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653819 834_E6_hg38_chr6:78745244-78745266_HEK293_edited_rep_1 834_E6_hg38_chr6:78745244-78745266_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535951 834_D6_hg38_chr6:75205144-75205166_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653821 834_D6_hg38_chr6:75205144-75205166_HEK293_edited_rep_1 834_D6_hg38_chr6:75205144-75205166_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535952 834_C6_hg38_chr6:64549555-64549577_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653820 834_C6_hg38_chr6:64549555-64549577_HEK293_edited_rep_1 834_C6_hg38_chr6:64549555-64549577_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535953 834_B6_hg38_chr6:50927821-50927843_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653822 834_B6_hg38_chr6:50927821-50927843_HEK293_edited_rep_1 834_B6_hg38_chr6:50927821-50927843_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535954 1734_D2_hg38_chr16:32025808-32025830_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653823 1734_D2_hg38_chr16:32025808-32025830_HEK293_control_rep_1 1734_D2_hg38_chr16:32025808-32025830_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535955 1734_D1_hg38_chr10:130782552-130782574_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653825 1734_D1_hg38_chr10:130782552-130782574_HEK293_control_rep_1 1734_D1_hg38_chr10:130782552-130782574_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535956 1734_E1_hg38_chr8:22778068-22778090_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653824 1734_E1_hg38_chr8:22778068-22778090_HEK293_control_rep_1 1734_E1_hg38_chr8:22778068-22778090_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535957 840_H12_hg19_chr3:128295785-128295807_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653827 840_H12_hg19_chr3:128295785-128295807_HEK293_edited_rep_1 840_H12_hg19_chr3:128295785-128295807_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535958 1734_A1_hg38_chr19:55115749-55115771_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653826 1734_A1_hg38_chr19:55115749-55115771_HEK293_control_rep_1 1734_A1_hg38_chr19:55115749-55115771_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535959 1734_B1_hg38_chr6:36797687-36797709_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653831 1734_B1_hg38_chr6:36797687-36797709_HEK293_control_rep_1 1734_B1_hg38_chr6:36797687-36797709_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535960 1734_C1_hg38_chr13:105960563-105960585_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653833 1734_C1_hg38_chr13:105960563-105960585_HEK293_control_rep_1 1734_C1_hg38_chr13:105960563-105960585_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535961 840_D12_hg19_chr11:70330837-70330859_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653828 840_D12_hg19_chr11:70330837-70330859_HEK293_edited_rep_1 840_D12_hg19_chr11:70330837-70330859_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535962 840_E12_hg19_chr6:43468947-43468969_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653835 840_E12_hg19_chr6:43468947-43468969_HEK293_edited_rep_1 840_E12_hg19_chr6:43468947-43468969_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535963 840_F12_hg19_chr9:95711879-95711901_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653832 840_F12_hg19_chr9:95711879-95711901_HEK293_edited_rep_1 840_F12_hg19_chr9:95711879-95711901_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535964 840_G12_hg19_chr22:38483674-38483696_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653829 840_G12_hg19_chr22:38483674-38483696_HEK293_edited_rep_1 840_G12_hg19_chr22:38483674-38483696_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535965 840_G2_hg19_chrX:17811010-17811032_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653834 840_G2_hg19_chrX:17811010-17811032_HEK293_edited_rep_1 840_G2_hg19_chrX:17811010-17811032_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535966 840_F2_hg19_chr11:4621708-4621730_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653830 840_F2_hg19_chr11:4621708-4621730_HEK293_edited_rep_1 840_F2_hg19_chr11:4621708-4621730_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535967 837_D4_hg19_chr16:50344049-50344071_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653836 837_D4_hg19_chr16:50344049-50344071_HEK293_control_rep_1 837_D4_hg19_chr16:50344049-50344071_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535968 837_E4_hg19_chr8:6664341-6664363_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653837 837_E4_hg19_chr8:6664341-6664363_HEK293_control_rep_1 837_E4_hg19_chr8:6664341-6664363_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535969 837_H3_hg19_chr9:136892384-136892406_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653838 837_H3_hg19_chr9:136892384-136892406_HEK293_control_rep_1 837_H3_hg19_chr9:136892384-136892406_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535970 837_A4_hg19_chr20:980410-980432_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653840 837_A4_hg19_chr20:980410-980432_HEK293_control_rep_1 837_A4_hg19_chr20:980410-980432_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535971 837_B4_hg19_chr1:31859278-31859300_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653839 837_B4_hg19_chr1:31859278-31859300_HEK293_control_rep_1 837_B4_hg19_chr1:31859278-31859300_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535972 837_C4_hg19_chr19:58920698-58920720_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653841 837_C4_hg19_chr19:58920698-58920720_HEK293_control_rep_1 837_C4_hg19_chr19:58920698-58920720_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535973 837_D3_hg19_chr4:6455037-6455059_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653846 837_D3_hg19_chr4:6455037-6455059_HEK293_control_rep_1 837_D3_hg19_chr4:6455037-6455059_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535974 837_E3_hg19_chr5:857945-857967_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653842 837_E3_hg19_chr5:857945-857967_HEK293_control_rep_1 837_E3_hg19_chr5:857945-857967_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535975 837_F3_hg19_chr4:1224010-1224032_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653845 837_F3_hg19_chr4:1224010-1224032_HEK293_control_rep_1 837_F3_hg19_chr4:1224010-1224032_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535976 837_G3_hg19_chrX:48917318-48917340_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653843 837_G3_hg19_chrX:48917318-48917340_HEK293_control_rep_1 837_G3_hg19_chrX:48917318-48917340_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535977 834_G2_hg38_chr5:149449656-149449678_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653848 834_G2_hg38_chr5:149449656-149449678_HEK293_edited_rep_1 834_G2_hg38_chr5:149449656-149449678_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535978 834_F2_hg38_chr5:143642775-143642797_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653844 834_F2_hg38_chr5:143642775-143642797_HEK293_edited_rep_1 834_F2_hg38_chr5:143642775-143642797_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535979 834_E2_hg38_chr5:143063881-143063903_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653851 834_E2_hg38_chr5:143063881-143063903_HEK293_edited_rep_1 834_E2_hg38_chr5:143063881-143063903_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535980 834_D2_hg38_chr5:139883422-139883444_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653850 834_D2_hg38_chr5:139883422-139883444_HEK293_edited_rep_1 834_D2_hg38_chr5:139883422-139883444_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535981 834_C2_hg38_chr5:137497665-137497687_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653847 834_C2_hg38_chr5:137497665-137497687_HEK293_edited_rep_1 834_C2_hg38_chr5:137497665-137497687_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535982 834_B2_hg38_chr5:135246387-135246409_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653852 834_B2_hg38_chr5:135246387-135246409_HEK293_edited_rep_1 834_B2_hg38_chr5:135246387-135246409_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535983 834_A2_hg38_chr5:134964294-134964316_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653849 834_A2_hg38_chr5:134964294-134964316_HEK293_edited_rep_1 834_A2_hg38_chr5:134964294-134964316_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535984 835_E3_hg19_chr19:51960245-51960267_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653853 835_E3_hg19_chr19:51960245-51960267_HEK293_control_rep_1 835_E3_hg19_chr19:51960245-51960267_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535985 835_B3_hg19_chr17:20350474-20350496_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653854 835_B3_hg19_chr17:20350474-20350496_HEK293_control_rep_1 835_B3_hg19_chr17:20350474-20350496_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535986 835_C3_hg19_chrX:106857392-106857414_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653856 835_C3_hg19_chrX:106857392-106857414_HEK293_control_rep_1 835_C3_hg19_chrX:106857392-106857414_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535987 835_H2_hg19_chr9:35699142-35699164_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653855 835_H2_hg19_chr9:35699142-35699164_HEK293_control_rep_1 835_H2_hg19_chr9:35699142-35699164_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535988 835_A3_hg19_chr9:66954552-66954574_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653858 835_A3_hg19_chr9:66954552-66954574_HEK293_control_rep_1 835_A3_hg19_chr9:66954552-66954574_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535989 835_F2_hg19_chr12:56630908-56630930_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653857 835_F2_hg19_chr12:56630908-56630930_HEK293_control_rep_1 835_F2_hg19_chr12:56630908-56630930_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535990 835_G2_hg19_chr4:10245774-10245796_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653859 835_G2_hg19_chr4:10245774-10245796_HEK293_control_rep_1 835_G2_hg19_chr4:10245774-10245796_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535991 835_D2_hg19_chr1:9385356-9385378_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653860 835_D2_hg19_chr1:9385356-9385378_HEK293_control_rep_1 835_D2_hg19_chr1:9385356-9385378_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535992 835_E2_hg19_chr12:50284997-50285019_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653862 835_E2_hg19_chr12:50284997-50285019_HEK293_control_rep_1 835_E2_hg19_chr12:50284997-50285019_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535993 839_B9_hg19_chr2:27603837-27603859_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653864 839_B9_hg19_chr2:27603837-27603859_HEK293_edited_rep_1 839_B9_hg19_chr2:27603837-27603859_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535994 839_C9_hg19_chr8:23351234-23351256_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653861 839_C9_hg19_chr8:23351234-23351256_HEK293_edited_rep_1 839_C9_hg19_chr8:23351234-23351256_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535995 839_B8_hg19_chr4:182898567-182898589_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653863 839_B8_hg19_chr4:182898567-182898589_HEK293_edited_rep_1 839_B8_hg19_chr4:182898567-182898589_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535996 839_C8_hg19_chr16:84886878-84886900_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653865 839_C8_hg19_chr16:84886878-84886900_HEK293_edited_rep_1 839_C8_hg19_chr16:84886878-84886900_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535997 839_D8_hg19_chr15:96865161-96865183_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653866 839_D8_hg19_chr15:96865161-96865183_HEK293_edited_rep_1 839_D8_hg19_chr15:96865161-96865183_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535998 839_E8_hg19_chr2:148314533-148314555_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653867 839_E8_hg19_chr2:148314533-148314555_HEK293_edited_rep_1 839_E8_hg19_chr2:148314533-148314555_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4535999 839_F8_hg19_chr17:80776142-80776164_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653868 839_F8_hg19_chr17:80776142-80776164_HEK293_edited_rep_1 839_F8_hg19_chr17:80776142-80776164_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536000 839_G8_hg19_chr2:101551413-101551435_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653869 839_G8_hg19_chr2:101551413-101551435_HEK293_edited_rep_1 839_G8_hg19_chr2:101551413-101551435_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536001 839_H8_hg19_chr12:9540554-9540576_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653870 839_H8_hg19_chr12:9540554-9540576_HEK293_edited_rep_1 839_H8_hg19_chr12:9540554-9540576_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536002 839_A9_hg19_chr7:151103009-151103031_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653873 839_A9_hg19_chr7:151103009-151103031_HEK293_edited_rep_1 839_A9_hg19_chr7:151103009-151103031_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536003 1734_A8_hg38_chr10:67488114-67488136_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653872 1734_A8_hg38_chr10:67488114-67488136_HEK293_control_rep_1 1734_A8_hg38_chr10:67488114-67488136_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536004 840_C12_hg19_chr1:113235335-113235357_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653871 840_C12_hg19_chr1:113235335-113235357_HEK293_edited_rep_1 840_C12_hg19_chr1:113235335-113235357_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536005 835_G7_hg19_chr6:117923725-117923747_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653875 835_G7_hg19_chr6:117923725-117923747_HEK293_control_rep_1 835_G7_hg19_chr6:117923725-117923747_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536006 835_F7_hg19_chr5:149650770-149650792_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653874 835_F7_hg19_chr5:149650770-149650792_HEK293_control_rep_1 835_F7_hg19_chr5:149650770-149650792_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536007 835_E7_hg19_chr16:1153458-1153480_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653879 835_E7_hg19_chr16:1153458-1153480_HEK293_control_rep_1 835_E7_hg19_chr16:1153458-1153480_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536008 835_D7_hg19_chr2:27039862-27039884_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653877 835_D7_hg19_chr2:27039862-27039884_HEK293_control_rep_1 835_D7_hg19_chr2:27039862-27039884_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536009 835_C8_hg19_chr11:78498152-78498174_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653876 835_C8_hg19_chr11:78498152-78498174_HEK293_control_rep_1 835_C8_hg19_chr11:78498152-78498174_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536010 835_B8_hg19_chr2:69726053-69726075_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653878 835_B8_hg19_chr2:69726053-69726075_HEK293_control_rep_1 835_B8_hg19_chr2:69726053-69726075_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536011 835_A8_hg19_chr19:10753884-10753906_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654020 835_A8_hg19_chr19:10753884-10753906_HEK293_control_rep_1 835_A8_hg19_chr19:10753884-10753906_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536012 835_H7_hg19_chr8:21752718-21752740_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653880 835_H7_hg19_chr8:21752718-21752740_HEK293_control_rep_1 835_H7_hg19_chr8:21752718-21752740_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536013 835_E8_hg19_chr1:40144280-40144302_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653881 835_E8_hg19_chr1:40144280-40144302_HEK293_control_rep_1 835_E8_hg19_chr1:40144280-40144302_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536014 835_D8_hg19_chrX:69249871-69249893_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653884 835_D8_hg19_chrX:69249871-69249893_HEK293_control_rep_1 835_D8_hg19_chrX:69249871-69249893_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536015 838_C7_hg19_chr17:4423146-4423168_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653883 838_C7_hg19_chr17:4423146-4423168_HEK293_edited_rep_1 838_C7_hg19_chr17:4423146-4423168_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536016 838_B7_hg19_chr22:36924541-36924563_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653882 838_B7_hg19_chr22:36924541-36924563_HEK293_edited_rep_1 838_B7_hg19_chr22:36924541-36924563_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536017 1734_F8_hg38_chr9:121763919-121763941_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653886 1734_F8_hg38_chr9:121763919-121763941_HEK293_control_rep_1 1734_F8_hg38_chr9:121763919-121763941_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536018 838_E6_hg19_chrX:65856095-65856117_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653885 838_E6_hg19_chrX:65856095-65856117_HEK293_edited_rep_1 838_E6_hg19_chrX:65856095-65856117_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536019 838_D6_hg19_chr11:118039016-118039038_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653890 838_D6_hg19_chr11:118039016-118039038_HEK293_edited_rep_1 838_D6_hg19_chr11:118039016-118039038_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536020 840_E11_hg19_chr1:18132409-18132431_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653889 840_E11_hg19_chr1:18132409-18132431_HEK293_edited_rep_1 840_E11_hg19_chr1:18132409-18132431_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536021 838_G6_hg19_chr17:71928063-71928085_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653887 838_G6_hg19_chr17:71928063-71928085_HEK293_edited_rep_1 838_G6_hg19_chr17:71928063-71928085_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536022 840_C7_hg19_chr15:41059404-41059426_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653888 840_C7_hg19_chr15:41059404-41059426_HEK293_edited_rep_1 840_C7_hg19_chr15:41059404-41059426_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536023 838_F6_hg19_chr16:67036555-67036577_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653891 838_F6_hg19_chr16:67036555-67036577_HEK293_edited_rep_1 838_F6_hg19_chr16:67036555-67036577_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536024 840_D3_hg19_chr4:6455037-6455059_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653894 840_D3_hg19_chr4:6455037-6455059_HEK293_edited_rep_1 840_D3_hg19_chr4:6455037-6455059_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536025 838_C9_hg19_chr15:25467895-25467917_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653893 838_C9_hg19_chr15:25467895-25467917_HEK293_edited_rep_1 838_C9_hg19_chr15:25467895-25467917_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536026 832_H6_hg38_chr2:153456216-153456238_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653892 832_H6_hg38_chr2:153456216-153456238_HEK293_edited_rep_1 832_H6_hg38_chr2:153456216-153456238_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536027 832_A7_hg38_chr2:156150310-156150332_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653895 832_A7_hg38_chr2:156150310-156150332_HEK293_edited_rep_1 832_A7_hg38_chr2:156150310-156150332_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536028 832_B6_hg38_chr2:128442226-128442248_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653896 832_B6_hg38_chr2:128442226-128442248_HEK293_edited_rep_1 832_B6_hg38_chr2:128442226-128442248_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536029 832_C6_hg38_chr2:129016603-129016625_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653899 832_C6_hg38_chr2:129016603-129016625_HEK293_edited_rep_1 832_C6_hg38_chr2:129016603-129016625_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536030 832_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653897 832_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 832_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536031 832_A6_hg38_chr2:128350804-128350826_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653900 832_A6_hg38_chr2:128350804-128350826_HEK293_edited_rep_1 832_A6_hg38_chr2:128350804-128350826_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536032 832_F6_hg38_chr2:149457516-149457538_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653898 832_F6_hg38_chr2:149457516-149457538_HEK293_edited_rep_1 832_F6_hg38_chr2:149457516-149457538_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536033 832_G6_hg38_chr2:152565324-152565346_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653901 832_G6_hg38_chr2:152565324-152565346_HEK293_edited_rep_1 832_G6_hg38_chr2:152565324-152565346_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536034 832_D6_hg38_chr2:136745425-136745447_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653904 832_D6_hg38_chr2:136745425-136745447_HEK293_edited_rep_1 832_D6_hg38_chr2:136745425-136745447_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536035 832_E6_hg38_chr2:146449691-146449713_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653903 832_E6_hg38_chr2:146449691-146449713_HEK293_edited_rep_1 832_E6_hg38_chr2:146449691-146449713_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536036 838_B3_hg19_chr17:20350474-20350496_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653902 838_B3_hg19_chr17:20350474-20350496_HEK293_edited_rep_1 838_B3_hg19_chr17:20350474-20350496_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536037 838_C3_hg19_chrX:106857392-106857414_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653905 838_C3_hg19_chrX:106857392-106857414_HEK293_edited_rep_1 838_C3_hg19_chrX:106857392-106857414_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536038 838_D3_hg19_chr5:38575912-38575934_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653907 838_D3_hg19_chr5:38575912-38575934_HEK293_edited_rep_1 838_D3_hg19_chr5:38575912-38575934_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536039 838_E3_hg19_chr19:51960245-51960267_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653906 838_E3_hg19_chr19:51960245-51960267_HEK293_edited_rep_1 838_E3_hg19_chr19:51960245-51960267_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536040 838_F2_hg19_chr12:56630908-56630930_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653908 838_F2_hg19_chr12:56630908-56630930_HEK293_edited_rep_1 838_F2_hg19_chr12:56630908-56630930_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536041 838_G2_hg19_chr4:10245774-10245796_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653909 838_G2_hg19_chr4:10245774-10245796_HEK293_edited_rep_1 838_G2_hg19_chr4:10245774-10245796_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536042 838_H2_hg19_chr9:35699142-35699164_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653910 838_H2_hg19_chr9:35699142-35699164_HEK293_edited_rep_1 838_H2_hg19_chr9:35699142-35699164_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536043 838_A3_hg19_chr9:66954552-66954574_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654055 838_A3_hg19_chr9:66954552-66954574_HEK293_edited_rep_1 838_A3_hg19_chr9:66954552-66954574_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536044 838_E8_hg19_chr1:40144280-40144302_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653912 838_E8_hg19_chr1:40144280-40144302_HEK293_edited_rep_1 838_E8_hg19_chr1:40144280-40144302_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536045 838_D8_hg19_chrX:69249871-69249893_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653911 838_D8_hg19_chrX:69249871-69249893_HEK293_edited_rep_1 838_D8_hg19_chrX:69249871-69249893_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536046 838_C8_hg19_chr11:78498152-78498174_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653913 838_C8_hg19_chr11:78498152-78498174_HEK293_edited_rep_1 838_C8_hg19_chr11:78498152-78498174_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536047 838_B8_hg19_chr2:69726053-69726075_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653918 838_B8_hg19_chr2:69726053-69726075_HEK293_edited_rep_1 838_B8_hg19_chr2:69726053-69726075_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536048 838_F3_hg19_chr1:6361008-6361030_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653917 838_F3_hg19_chr1:6361008-6361030_HEK293_edited_rep_1 838_F3_hg19_chr1:6361008-6361030_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536049 838_G3_hg19_chr11:46384521-46384543_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653914 838_G3_hg19_chr11:46384521-46384543_HEK293_edited_rep_1 838_G3_hg19_chr11:46384521-46384543_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536050 838_G7_hg19_chr6:117923725-117923747_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653915 838_G7_hg19_chr6:117923725-117923747_HEK293_edited_rep_1 838_G7_hg19_chr6:117923725-117923747_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536051 838_F7_hg19_chr5:149650770-149650792_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653916 838_F7_hg19_chr5:149650770-149650792_HEK293_edited_rep_1 838_F7_hg19_chr5:149650770-149650792_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536052 836_D2_hg19_chr6:33737867-33737889_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653919 836_D2_hg19_chr6:33737867-33737889_HEK293_control_rep_1 836_D2_hg19_chr6:33737867-33737889_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536053 836_E2_hg19_chrX:48370088-48370110_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653921 836_E2_hg19_chrX:48370088-48370110_HEK293_control_rep_1 836_E2_hg19_chrX:48370088-48370110_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536054 836_B2_hg19_chr11:61111237-61111259_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653922 836_B2_hg19_chr11:61111237-61111259_HEK293_control_rep_1 836_B2_hg19_chr11:61111237-61111259_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536055 836_C2_hg19_chr1:7448860-7448882_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653920 836_C2_hg19_chr1:7448860-7448882_HEK293_control_rep_1 836_C2_hg19_chr1:7448860-7448882_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536056 836_H1_hg19_chr6:37592409-37592431_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653924 836_H1_hg19_chr6:37592409-37592431_HEK293_control_rep_1 836_H1_hg19_chr6:37592409-37592431_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536057 836_A2_hg19_chr10:72079191-72079213_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653923 836_A2_hg19_chr10:72079191-72079213_HEK293_control_rep_1 836_A2_hg19_chr10:72079191-72079213_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536058 836_F1_hg19_chr20:45321341-45321363_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654078 836_F1_hg19_chr20:45321341-45321363_HEK293_control_rep_1 836_F1_hg19_chr20:45321341-45321363_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536059 836_G1_hg19_chr3:172018373-172018395_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653925 836_G1_hg19_chr3:172018373-172018395_HEK293_control_rep_1 836_G1_hg19_chr3:172018373-172018395_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536060 836_F2_hg19_chr6:41739322-41739344_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653926 836_F2_hg19_chr6:41739322-41739344_HEK293_control_rep_1 836_F2_hg19_chr6:41739322-41739344_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536061 836_G2_hg19_chr19:34111061-34111083_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653929 836_G2_hg19_chr19:34111061-34111083_HEK293_control_rep_1 836_G2_hg19_chr19:34111061-34111083_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536062 831_D12_hg38_chr19:15042603-15042625_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653931 831_D12_hg38_chr19:15042603-15042625_HEK293_edited_rep_1 831_D12_hg38_chr19:15042603-15042625_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536063 833_G2_hg38_chr22:41280741-41280763_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653927 833_G2_hg38_chr22:41280741-41280763_HEK293_edited_rep_1 833_G2_hg38_chr22:41280741-41280763_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536064 833_F2_hg38_chr22:41213974-41213996_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653928 833_F2_hg38_chr22:41213974-41213996_HEK293_edited_rep_1 833_F2_hg38_chr22:41213974-41213996_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536065 833_A3_hg38_chr22:42306467-42306489_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653930 833_A3_hg38_chr22:42306467-42306489_HEK293_edited_rep_1 833_A3_hg38_chr22:42306467-42306489_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536066 833_H2_hg38_chr22:41337455-41337477_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653934 833_H2_hg38_chr22:41337455-41337477_HEK293_edited_rep_1 833_H2_hg38_chr22:41337455-41337477_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536067 833_C3_hg38_chr22:45232665-45232687_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653933 833_C3_hg38_chr22:45232665-45232687_HEK293_edited_rep_1 833_C3_hg38_chr22:45232665-45232687_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536068 833_B3_hg38_chr22:44661384-44661406_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653932 833_B3_hg38_chr22:44661384-44661406_HEK293_edited_rep_1 833_B3_hg38_chr22:44661384-44661406_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536069 833_E3_hg38_chr22:46094086-46094108_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653935 833_E3_hg38_chr22:46094086-46094108_HEK293_edited_rep_1 833_E3_hg38_chr22:46094086-46094108_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536070 833_D3_hg38_chr22:45913523-45913545_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653936 833_D3_hg38_chr22:45913523-45913545_HEK293_edited_rep_1 833_D3_hg38_chr22:45913523-45913545_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536071 833_G3_hg38_chr22:48540162-48540184_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653938 833_G3_hg38_chr22:48540162-48540184_HEK293_edited_rep_1 833_G3_hg38_chr22:48540162-48540184_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536072 833_F3_hg38_chr22:48144700-48144722_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653943 833_F3_hg38_chr22:48144700-48144722_HEK293_edited_rep_1 833_F3_hg38_chr22:48144700-48144722_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536073 833_A10_hg38_chr4:4502860-4502882_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653937 833_A10_hg38_chr4:4502860-4502882_HEK293_edited_rep_1 833_A10_hg38_chr4:4502860-4502882_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536074 833_H9_hg38_chr4:2512230-2512252_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653941 833_H9_hg38_chr4:2512230-2512252_HEK293_edited_rep_1 833_H9_hg38_chr4:2512230-2512252_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536075 833_C9_hg38_chr3:143266845-143266867_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653944 833_C9_hg38_chr3:143266845-143266867_HEK293_edited_rep_1 833_C9_hg38_chr3:143266845-143266867_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536076 833_B9_hg38_chr3:140756536-140756558_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653939 833_B9_hg38_chr3:140756536-140756558_HEK293_edited_rep_1 833_B9_hg38_chr3:140756536-140756558_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536077 833_A9_hg38_chr3:129564812-129564834_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653942 833_A9_hg38_chr3:129564812-129564834_HEK293_edited_rep_1 833_A9_hg38_chr3:129564812-129564834_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536078 833_H8_hg38_chr3:128565472-128565494_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653940 833_H8_hg38_chr3:128565472-128565494_HEK293_edited_rep_1 833_H8_hg38_chr3:128565472-128565494_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536079 833_G9_hg38_chr3:196144387-196144409_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653945 833_G9_hg38_chr3:196144387-196144409_HEK293_edited_rep_1 833_G9_hg38_chr3:196144387-196144409_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536080 833_F9_hg38_chr3:196128744-196128766_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653947 833_F9_hg38_chr3:196128744-196128766_HEK293_edited_rep_1 833_F9_hg38_chr3:196128744-196128766_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536081 833_E9_hg38_chr3:166849798-166849820_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653949 833_E9_hg38_chr3:166849798-166849820_HEK293_edited_rep_1 833_E9_hg38_chr3:166849798-166849820_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536082 833_D9_hg38_chr3:155908221-155908243_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653950 833_D9_hg38_chr3:155908221-155908243_HEK293_edited_rep_1 833_D9_hg38_chr3:155908221-155908243_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536083 834_F9_hg38_chr7:35756056-35756078_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653952 834_F9_hg38_chr7:35756056-35756078_HEK293_edited_rep_1 834_F9_hg38_chr7:35756056-35756078_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536084 834_G9_hg38_chr7:41036723-41036745_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653951 834_G9_hg38_chr7:41036723-41036745_HEK293_edited_rep_1 834_G9_hg38_chr7:41036723-41036745_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536085 834_D9_hg38_chr7:29041407-29041429_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653954 834_D9_hg38_chr7:29041407-29041429_HEK293_edited_rep_1 834_D9_hg38_chr7:29041407-29041429_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536086 834_E9_hg38_chr7:32362454-32362476_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653953 834_E9_hg38_chr7:32362454-32362476_HEK293_edited_rep_1 834_E9_hg38_chr7:32362454-32362476_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536087 834_B10_hg38_chr7:55270994-55271016_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653955 834_B10_hg38_chr7:55270994-55271016_HEK293_edited_rep_1 834_B10_hg38_chr7:55270994-55271016_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536088 834_C10_hg38_chr7:73839789-73839811_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653956 834_C10_hg38_chr7:73839789-73839811_HEK293_edited_rep_1 834_C10_hg38_chr7:73839789-73839811_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536089 834_H9_hg38_chr7:45651409-45651431_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653957 834_H9_hg38_chr7:45651409-45651431_HEK293_edited_rep_1 834_H9_hg38_chr7:45651409-45651431_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536090 834_A10_hg38_chr7:51300790-51300812_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653958 834_A10_hg38_chr7:51300790-51300812_HEK293_edited_rep_1 834_A10_hg38_chr7:51300790-51300812_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536091 840_F1_hg19_chr2:121716009-121716031_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653959 840_F1_hg19_chr2:121716009-121716031_HEK293_edited_rep_1 840_F1_hg19_chr2:121716009-121716031_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536092 840_G1_hg19_chr17:38508360-38508382_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653961 840_G1_hg19_chr17:38508360-38508382_HEK293_edited_rep_1 840_G1_hg19_chr17:38508360-38508382_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536093 834_D10_hg38_chr7:74291350-74291372_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653960 834_D10_hg38_chr7:74291350-74291372_HEK293_edited_rep_1 834_D10_hg38_chr7:74291350-74291372_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536094 834_E10_hg38_chr7:80981634-80981656_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653962 834_E10_hg38_chr7:80981634-80981656_HEK293_edited_rep_1 834_E10_hg38_chr7:80981634-80981656_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536095 840_B1_hg19_chr3:71744235-71744257_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653963 840_B1_hg19_chr3:71744235-71744257_HEK293_edited_rep_1 840_B1_hg19_chr3:71744235-71744257_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536096 840_C1_hg19_chr6:137307894-137307916_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653964 840_C1_hg19_chr6:137307894-137307916_HEK293_edited_rep_1 840_C1_hg19_chr6:137307894-137307916_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536097 840_D1_hg19_chr3:25347412-25347434_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653965 840_D1_hg19_chr3:25347412-25347434_HEK293_edited_rep_1 840_D1_hg19_chr3:25347412-25347434_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536098 840_E1_hg19_chr15:66292443-66292465_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653967 840_E1_hg19_chr15:66292443-66292465_HEK293_edited_rep_1 840_E1_hg19_chr15:66292443-66292465_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536099 1734_A5_hg38_chr22:46558212-46558234_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653966 1734_A5_hg38_chr22:46558212-46558234_HEK293_control_rep_1 1734_A5_hg38_chr22:46558212-46558234_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536100 1734_H4_hg38_chr2:16011535-16011557_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653968 1734_H4_hg38_chr2:16011535-16011557_HEK293_control_rep_1 1734_H4_hg38_chr2:16011535-16011557_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536101 1734_G4_hg38_chr4:22171983-22172005_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653969 1734_G4_hg38_chr4:22171983-22172005_HEK293_control_rep_1 1734_G4_hg38_chr4:22171983-22172005_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536102 1734_F4_hg38_chr15:25222748-25222770_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653970 1734_F4_hg38_chr15:25222748-25222770_HEK293_control_rep_1 1734_F4_hg38_chr15:25222748-25222770_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536103 831_D11_hg38_chr19:4278965-4278987_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653972 831_D11_hg38_chr19:4278965-4278987_HEK293_edited_rep_1 831_D11_hg38_chr19:4278965-4278987_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536104 831_E11_hg38_chr19:4442691-4442713_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653971 831_E11_hg38_chr19:4442691-4442713_HEK293_edited_rep_1 831_E11_hg38_chr19:4442691-4442713_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536105 838_B6_hg19_chr6:36765464-36765486_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653974 838_B6_hg19_chr6:36765464-36765486_HEK293_edited_rep_1 838_B6_hg19_chr6:36765464-36765486_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536106 838_C6_hg19_chr4:170332211-170332233_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653973 838_C6_hg19_chr4:170332211-170332233_HEK293_edited_rep_1 838_C6_hg19_chr4:170332211-170332233_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536107 831_H10_hg38_chr18:73989311-73989333_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653975 831_H10_hg38_chr18:73989311-73989333_HEK293_edited_rep_1 831_H10_hg38_chr18:73989311-73989333_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536108 831_A11_hg38_chr18:77394923-77394945_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653976 831_A11_hg38_chr18:77394923-77394945_HEK293_edited_rep_1 831_A11_hg38_chr18:77394923-77394945_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536109 831_B11_hg38_chr19:1883677-1883699_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653979 831_B11_hg38_chr19:1883677-1883699_HEK293_edited_rep_1 831_B11_hg38_chr19:1883677-1883699_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536110 831_C11_hg38_chr19:2000302-2000324_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653977 831_C11_hg38_chr19:2000302-2000324_HEK293_edited_rep_1 831_C11_hg38_chr19:2000302-2000324_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536111 831_D10_hg38_chr18:31724444-31724466_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653978 831_D10_hg38_chr18:31724444-31724466_HEK293_edited_rep_1 831_D10_hg38_chr18:31724444-31724466_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536112 831_E10_hg38_chr18:37253000-37253022_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653980 831_E10_hg38_chr18:37253000-37253022_HEK293_edited_rep_1 831_E10_hg38_chr18:37253000-37253022_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536113 831_F10_hg38_chr18:38580676-38580698_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653981 831_F10_hg38_chr18:38580676-38580698_HEK293_edited_rep_1 831_F10_hg38_chr18:38580676-38580698_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536114 831_G10_hg38_chr18:39233088-39233110_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653982 831_G10_hg38_chr18:39233088-39233110_HEK293_edited_rep_1 831_G10_hg38_chr18:39233088-39233110_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536115 837_E12_hg19_chr6:43468947-43468969_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653983 837_E12_hg19_chr6:43468947-43468969_HEK293_control_rep_1 837_E12_hg19_chr6:43468947-43468969_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536116 837_F1_hg19_chr2:121716009-121716031_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653984 837_F1_hg19_chr2:121716009-121716031_HEK293_control_rep_1 837_F1_hg19_chr2:121716009-121716031_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536117 840_H4_hg19_chr16:89774898-89774920_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653985 840_H4_hg19_chr16:89774898-89774920_HEK293_edited_rep_1 840_H4_hg19_chr16:89774898-89774920_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536118 837_G1_hg19_chr17:38508360-38508382_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653989 837_G1_hg19_chr17:38508360-38508382_HEK293_control_rep_1 837_G1_hg19_chr17:38508360-38508382_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536119 836_A10_hg19_chr1:221108290-221108312_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653986 836_A10_hg19_chr1:221108290-221108312_HEK293_control_rep_1 836_A10_hg19_chr1:221108290-221108312_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536120 836_H9_hg19_chr19:8957091-8957113_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653987 836_H9_hg19_chr19:8957091-8957113_HEK293_control_rep_1 836_H9_hg19_chr19:8957091-8957113_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536121 836_G9_hg19_chr8:57454928-57454950_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653990 836_G9_hg19_chr8:57454928-57454950_HEK293_control_rep_1 836_G9_hg19_chr8:57454928-57454950_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536122 836_F9_hg19_chr19:51871850-51871872_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653988 836_F9_hg19_chr19:51871850-51871872_HEK293_control_rep_1 836_F9_hg19_chr19:51871850-51871872_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536123 836_E9_hg19_chr22:37948800-37948822_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653991 836_E9_hg19_chr22:37948800-37948822_HEK293_control_rep_1 836_E9_hg19_chr22:37948800-37948822_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536124 836_D9_hg19_chr12:133249100-133249122_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653992 836_D9_hg19_chr12:133249100-133249122_HEK293_control_rep_1 836_D9_hg19_chr12:133249100-133249122_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536125 833_B11_hg38_chr4:94953809-94953831_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653994 833_B11_hg38_chr4:94953809-94953831_HEK293_edited_rep_1 833_B11_hg38_chr4:94953809-94953831_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536126 833_C11_hg38_chr4:129565143-129565165_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653993 833_C11_hg38_chr4:129565143-129565165_HEK293_edited_rep_1 833_C11_hg38_chr4:129565143-129565165_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536127 833_H10_hg38_chr4:63359424-63359446_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653996 833_H10_hg38_chr4:63359424-63359446_HEK293_edited_rep_1 833_H10_hg38_chr4:63359424-63359446_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536128 833_A11_hg38_chr4:72568805-72568827_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653995 833_A11_hg38_chr4:72568805-72568827_HEK293_edited_rep_1 833_A11_hg38_chr4:72568805-72568827_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536129 833_F10_hg38_chr4:51867079-51867101_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653999 833_F10_hg38_chr4:51867079-51867101_HEK293_edited_rep_1 833_F10_hg38_chr4:51867079-51867101_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536130 833_G10_hg38_chr4:56857375-56857397_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654002 833_G10_hg38_chr4:56857375-56857397_HEK293_edited_rep_1 833_G10_hg38_chr4:56857375-56857397_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536131 833_D10_hg38_chr4:28795449-28795471_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654003 833_D10_hg38_chr4:28795449-28795471_HEK293_edited_rep_1 833_D10_hg38_chr4:28795449-28795471_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536132 833_E10_hg38_chr4:40650162-40650184_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654000 833_E10_hg38_chr4:40650162-40650184_HEK293_edited_rep_1 833_E10_hg38_chr4:40650162-40650184_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536133 833_B10_hg38_chr4:11612286-11612308_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653997 833_B10_hg38_chr4:11612286-11612308_HEK293_edited_rep_1 833_B10_hg38_chr4:11612286-11612308_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536134 833_C10_hg38_chr4:25978124-25978146_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3653998 833_C10_hg38_chr4:25978124-25978146_HEK293_edited_rep_1 833_C10_hg38_chr4:25978124-25978146_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536135 837_H1_hg19_chr8:17484307-17484329_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654001 837_H1_hg19_chr8:17484307-17484329_HEK293_control_rep_1 837_H1_hg19_chr8:17484307-17484329_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536136 837_A2_hg19_chr5:169511413-169511435_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654004 837_A2_hg19_chr5:169511413-169511435_HEK293_control_rep_1 837_A2_hg19_chr5:169511413-169511435_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536137 1734_H2_hg38_chr16:32969369-32969391_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654005 1734_H2_hg38_chr16:32969369-32969391_HEK293_control_rep_1 1734_H2_hg38_chr16:32969369-32969391_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536138 839_A1_hg19_chr21:45401548-45401570_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654008 839_A1_hg19_chr21:45401548-45401570_HEK293_edited_rep_1 839_A1_hg19_chr21:45401548-45401570_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536139 838_H12_hg19_chr10:121266568-121266590_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654007 838_H12_hg19_chr10:121266568-121266590_HEK293_edited_rep_1 838_H12_hg19_chr10:121266568-121266590_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536140 835_F9_hg19_chr16:33687030-33687052_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654006 835_F9_hg19_chr16:33687030-33687052_HEK293_control_rep_1 835_F9_hg19_chr16:33687030-33687052_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536141 835_G9_hg19_chr2:28510827-28510849_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654010 835_G9_hg19_chr2:28510827-28510849_HEK293_control_rep_1 835_G9_hg19_chr2:28510827-28510849_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536142 839_E1_hg19_chr9:131258152-131258174_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654009 839_E1_hg19_chr9:131258152-131258174_HEK293_edited_rep_1 839_E1_hg19_chr9:131258152-131258174_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536143 839_D1_hg19_chr1:231074350-231074372_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654011 839_D1_hg19_chr1:231074350-231074372_HEK293_edited_rep_1 839_D1_hg19_chr1:231074350-231074372_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536144 839_C1_hg19_chr3:38639828-38639850_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654012 839_C1_hg19_chr3:38639828-38639850_HEK293_edited_rep_1 839_C1_hg19_chr3:38639828-38639850_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536145 839_B1_hg19_chr2:26762118-26762140_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654014 839_B1_hg19_chr2:26762118-26762140_HEK293_edited_rep_1 839_B1_hg19_chr2:26762118-26762140_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536146 835_H8_hg19_chr13:111287204-111287226_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654013 835_H8_hg19_chr13:111287204-111287226_HEK293_control_rep_1 835_H8_hg19_chr13:111287204-111287226_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536147 835_A9_hg19_chr9:39193157-39193179_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654015 835_A9_hg19_chr9:39193157-39193179_HEK293_control_rep_1 835_A9_hg19_chr9:39193157-39193179_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536148 835_F8_hg19_chr15:65693902-65693924_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654016 835_F8_hg19_chr15:65693902-65693924_HEK293_control_rep_1 835_F8_hg19_chr15:65693902-65693924_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536149 835_G8_hg19_chr1:5708155-5708177_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654019 835_G8_hg19_chr1:5708155-5708177_HEK293_control_rep_1 835_G8_hg19_chr1:5708155-5708177_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536150 835_D9_hg19_chr4:1718259-1718281_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654017 835_D9_hg19_chr4:1718259-1718281_HEK293_control_rep_1 835_D9_hg19_chr4:1718259-1718281_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536151 835_E9_hg19_chr12:132311857-132311879_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654018 835_E9_hg19_chr12:132311857-132311879_HEK293_control_rep_1 835_E9_hg19_chr12:132311857-132311879_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536152 835_B9_hg19_chr9:39722459-39722481_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654021 835_B9_hg19_chr9:39722459-39722481_HEK293_control_rep_1 835_B9_hg19_chr9:39722459-39722481_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536153 835_C9_hg19_chr15:25467895-25467917_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654022 835_C9_hg19_chr15:25467895-25467917_HEK293_control_rep_1 835_C9_hg19_chr15:25467895-25467917_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536154 837_D12_hg19_chr11:70330837-70330859_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654023 837_D12_hg19_chr11:70330837-70330859_HEK293_control_rep_1 837_D12_hg19_chr11:70330837-70330859_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536155 840_H10_hg19_chr1:18451047-18451069_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654024 840_H10_hg19_chr1:18451047-18451069_HEK293_edited_rep_1 840_H10_hg19_chr1:18451047-18451069_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536156 835_A4_hg19_chr19:35022998-35023020_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654027 835_A4_hg19_chr19:35022998-35023020_HEK293_control_rep_1 835_A4_hg19_chr19:35022998-35023020_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536157 835_H3_hg19_chr19:55627117-55627139_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654025 835_H3_hg19_chr19:55627117-55627139_HEK293_control_rep_1 835_H3_hg19_chr19:55627117-55627139_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536158 835_G3_hg19_chr11:46384521-46384543_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654026 835_G3_hg19_chr11:46384521-46384543_HEK293_control_rep_1 835_G3_hg19_chr11:46384521-46384543_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536159 835_F3_hg19_chr1:6361008-6361030_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654028 835_F3_hg19_chr1:6361008-6361030_HEK293_control_rep_1 835_F3_hg19_chr1:6361008-6361030_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536160 835_E4_hg19_chr16:31484903-31484925_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654030 835_E4_hg19_chr16:31484903-31484925_HEK293_control_rep_1 835_E4_hg19_chr16:31484903-31484925_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536161 835_D4_hg19_chr17:43334265-43334287_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654033 835_D4_hg19_chr17:43334265-43334287_HEK293_control_rep_1 835_D4_hg19_chr17:43334265-43334287_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536162 835_C4_hg19_chr9:130268812-130268834_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654029 835_C4_hg19_chr9:130268812-130268834_HEK293_control_rep_1 835_C4_hg19_chr9:130268812-130268834_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536163 835_B4_hg19_chr5:858465-858487_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654031 835_B4_hg19_chr5:858465-858487_HEK293_control_rep_1 835_B4_hg19_chr5:858465-858487_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536164 835_G4_hg19_chr7:30946099-30946121_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654035 835_G4_hg19_chr7:30946099-30946121_HEK293_control_rep_1 835_G4_hg19_chr7:30946099-30946121_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536165 835_F4_hg19_chrX:11701311-11701333_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654032 835_F4_hg19_chrX:11701311-11701333_HEK293_control_rep_1 835_F4_hg19_chrX:11701311-11701333_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536166 838_B9_hg19_chr9:39722459-39722481_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654034 838_B9_hg19_chr9:39722459-39722481_HEK293_edited_rep_1 838_B9_hg19_chr9:39722459-39722481_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536167 1734_E5_hg38_chr5:858258-858280_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654036 1734_E5_hg38_chr5:858258-858280_HEK293_control_rep_1 1734_E5_hg38_chr5:858258-858280_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536168 832_C10_hg38_chr20:47774443-47774465_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654038 832_C10_hg38_chr20:47774443-47774465_HEK293_edited_rep_1 832_C10_hg38_chr20:47774443-47774465_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536169 832_B10_hg38_chr20:44465203-44465225_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654039 832_B10_hg38_chr20:44465203-44465225_HEK293_edited_rep_1 832_B10_hg38_chr20:44465203-44465225_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536170 832_E10_hg38_chr20:53794814-53794836_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654037 832_E10_hg38_chr20:53794814-53794836_HEK293_edited_rep_1 832_E10_hg38_chr20:53794814-53794836_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536171 832_D10_hg38_chr20:50874411-50874433_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654040 832_D10_hg38_chr20:50874411-50874433_HEK293_edited_rep_1 832_D10_hg38_chr20:50874411-50874433_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536172 832_G9_hg38_chr20:35274834-35274856_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654041 832_G9_hg38_chr20:35274834-35274856_HEK293_edited_rep_1 832_G9_hg38_chr20:35274834-35274856_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536173 832_F9_hg38_chr20:17610213-17610235_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654042 832_F9_hg38_chr20:17610213-17610235_HEK293_edited_rep_1 832_F9_hg38_chr20:17610213-17610235_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536174 832_A10_hg38_chr20:41832610-41832632_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654043 832_A10_hg38_chr20:41832610-41832632_HEK293_edited_rep_1 832_A10_hg38_chr20:41832610-41832632_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536175 832_H9_hg38_chr20:35294584-35294606_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654044 832_H9_hg38_chr20:35294584-35294606_HEK293_edited_rep_1 832_H9_hg38_chr20:35294584-35294606_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536176 837_G11_hg19_chr1:26373692-26373714_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654045 837_G11_hg19_chr1:26373692-26373714_HEK293_control_rep_1 837_G11_hg19_chr1:26373692-26373714_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536177 833_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654046 833_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 833_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536178 837_E11_hg19_chr1:18132409-18132431_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654049 837_E11_hg19_chr1:18132409-18132431_HEK293_control_rep_1 837_E11_hg19_chr1:18132409-18132431_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536179 837_D11_hg19_chr5:61100911-61100933_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654047 837_D11_hg19_chr5:61100911-61100933_HEK293_control_rep_1 837_D11_hg19_chr5:61100911-61100933_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536180 832_G10_hg38_chr20:57600294-57600316_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654048 832_G10_hg38_chr20:57600294-57600316_HEK293_edited_rep_1 832_G10_hg38_chr20:57600294-57600316_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536181 832_F10_hg38_chr20:57019211-57019233_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654050 832_F10_hg38_chr20:57019211-57019233_HEK293_edited_rep_1 832_F10_hg38_chr20:57019211-57019233_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536182 837_A11_hg19_chr19:17279586-17279608_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654051 837_A11_hg19_chr19:17279586-17279608_HEK293_control_rep_1 837_A11_hg19_chr19:17279586-17279608_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536183 833_A6_hg38_chr3:46499167-46499189_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654052 833_A6_hg38_chr3:46499167-46499189_HEK293_edited_rep_1 833_A6_hg38_chr3:46499167-46499189_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536184 838_B12_hg19_chr22:17529539-17529561_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654054 838_B12_hg19_chr22:17529539-17529561_HEK293_edited_rep_1 838_B12_hg19_chr22:17529539-17529561_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536185 838_C12_hg19_chr19:4817649-4817671_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654056 838_C12_hg19_chr19:4817649-4817671_HEK293_edited_rep_1 838_C12_hg19_chr19:4817649-4817671_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536186 838_H11_hg19_chr8:144885120-144885142_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654053 838_H11_hg19_chr8:144885120-144885142_HEK293_edited_rep_1 838_H11_hg19_chr8:144885120-144885142_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536187 838_A12_hg19_chr10:105463950-105463972_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654057 838_A12_hg19_chr10:105463950-105463972_HEK293_edited_rep_1 838_A12_hg19_chr10:105463950-105463972_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536188 838_F11_hg19_chr1:48417872-48417894_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654058 838_F11_hg19_chr1:48417872-48417894_HEK293_edited_rep_1 838_F11_hg19_chr1:48417872-48417894_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536189 838_G11_hg19_chr17:26958788-26958810_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654060 838_G11_hg19_chr17:26958788-26958810_HEK293_edited_rep_1 838_G11_hg19_chr17:26958788-26958810_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536190 838_D11_hg19_chr16:17399046-17399068_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654061 838_D11_hg19_chr16:17399046-17399068_HEK293_edited_rep_1 838_D11_hg19_chr16:17399046-17399068_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536191 838_E11_hg19_chr10:132580816-132580838_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654063 838_E11_hg19_chr10:132580816-132580838_HEK293_edited_rep_1 838_E11_hg19_chr10:132580816-132580838_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536192 838_D12_hg19_chr14:90989236-90989258_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654059 838_D12_hg19_chr14:90989236-90989258_HEK293_edited_rep_1 838_D12_hg19_chr14:90989236-90989258_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536193 838_E12_hg19_chr8:144749069-144749091_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654062 838_E12_hg19_chr8:144749069-144749091_HEK293_edited_rep_1 838_E12_hg19_chr8:144749069-144749091_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536194 837_G5_hg19_chr11:117099065-117099087_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654064 837_G5_hg19_chr11:117099065-117099087_HEK293_control_rep_1 837_G5_hg19_chr11:117099065-117099087_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536195 838_H9_hg19_chr4:109424089-109424111_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654065 838_H9_hg19_chr4:109424089-109424111_HEK293_edited_rep_1 838_H9_hg19_chr4:109424089-109424111_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536196 840_D7_hg19_chr1:16318642-16318664_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654066 840_D7_hg19_chr1:16318642-16318664_HEK293_edited_rep_1 840_D7_hg19_chr1:16318642-16318664_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536197 838_A10_hg19_chr10:131639488-131639510_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654068 838_A10_hg19_chr10:131639488-131639510_HEK293_edited_rep_1 838_A10_hg19_chr10:131639488-131639510_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536198 833_B6_hg38_chr3:48603270-48603292_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654067 833_B6_hg38_chr3:48603270-48603292_HEK293_edited_rep_1 833_B6_hg38_chr3:48603270-48603292_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536199 831_C12_hg38_chr19:15012917-15012939_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654069 831_C12_hg38_chr19:15012917-15012939_HEK293_edited_rep_1 831_C12_hg38_chr19:15012917-15012939_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536200 831_B12_hg38_chr19:12549704-12549726_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654070 831_B12_hg38_chr19:12549704-12549726_HEK293_edited_rep_1 831_B12_hg38_chr19:12549704-12549726_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536201 831_E12_hg38_chr19:16218642-16218664_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654071 831_E12_hg38_chr19:16218642-16218664_HEK293_edited_rep_1 831_E12_hg38_chr19:16218642-16218664_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536202 833_C6_hg38_chr3:49461250-49461272_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654073 833_C6_hg38_chr3:49461250-49461272_HEK293_edited_rep_1 833_C6_hg38_chr3:49461250-49461272_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536203 831_G11_hg38_chr19:7773624-7773646_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654075 831_G11_hg38_chr19:7773624-7773646_HEK293_edited_rep_1 831_G11_hg38_chr19:7773624-7773646_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536204 831_F11_hg38_chr19:4944500-4944522_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654072 831_F11_hg38_chr19:4944500-4944522_HEK293_edited_rep_1 831_F11_hg38_chr19:4944500-4944522_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536205 831_A12_hg38_chr19:10119188-10119210_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654074 831_A12_hg38_chr19:10119188-10119210_HEK293_edited_rep_1 831_A12_hg38_chr19:10119188-10119210_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536206 831_H11_hg38_chr19:8085303-8085325_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654076 831_H11_hg38_chr19:8085303-8085325_HEK293_edited_rep_1 831_H11_hg38_chr19:8085303-8085325_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536207 838_H5_hg19_chr10:49999950-49999972_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654080 838_H5_hg19_chr10:49999950-49999972_HEK293_edited_rep_1 838_H5_hg19_chr10:49999950-49999972_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536208 831_G12_hg38_chr19:18415950-18415972_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654082 831_G12_hg38_chr19:18415950-18415972_HEK293_edited_rep_1 831_G12_hg38_chr19:18415950-18415972_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536209 831_F12_hg38_chr19:17642689-17642711_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654077 831_F12_hg38_chr19:17642689-17642711_HEK293_edited_rep_1 831_F12_hg38_chr19:17642689-17642711_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536210 837_F5_hg19_chr3:48562425-48562447_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654079 837_F5_hg19_chr3:48562425-48562447_HEK293_control_rep_1 837_F5_hg19_chr3:48562425-48562447_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536211 840_B6_hg19_chr1:181453844-181453866_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654083 840_B6_hg19_chr1:181453844-181453866_HEK293_edited_rep_1 840_B6_hg19_chr1:181453844-181453866_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536212 840_C6_hg19_chr15:44482442-44482464_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654084 840_C6_hg19_chr15:44482442-44482464_HEK293_edited_rep_1 840_C6_hg19_chr15:44482442-44482464_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536213 840_D6_hg19_chr5:2410480-2410502_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654081 840_D6_hg19_chr5:2410480-2410502_HEK293_edited_rep_1 840_D6_hg19_chr5:2410480-2410502_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536214 840_E6_hg19_chr11:119675039-119675061_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654086 840_E6_hg19_chr11:119675039-119675061_HEK293_edited_rep_1 840_E6_hg19_chr11:119675039-119675061_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536215 840_F6_hg19_chr14:23520110-23520132_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654085 840_F6_hg19_chr14:23520110-23520132_HEK293_edited_rep_1 840_F6_hg19_chr14:23520110-23520132_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536216 840_G6_hg19_chr12:9730868-9730890_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654087 840_G6_hg19_chr12:9730868-9730890_HEK293_edited_rep_1 840_G6_hg19_chr12:9730868-9730890_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536217 840_H6_hg19_chr19:50161823-50161845_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654088 840_H6_hg19_chr19:50161823-50161845_HEK293_edited_rep_1 840_H6_hg19_chr19:50161823-50161845_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536218 840_A7_hg19_chr3:13525177-13525199_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654089 840_A7_hg19_chr3:13525177-13525199_HEK293_edited_rep_1 840_A7_hg19_chr3:13525177-13525199_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536219 840_B7_hg19_chr11:125589348-125589370_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654091 840_B7_hg19_chr11:125589348-125589370_HEK293_edited_rep_1 840_B7_hg19_chr11:125589348-125589370_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536220 838_A6_hg19_chr11:77033596-77033618_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654092 838_A6_hg19_chr11:77033596-77033618_HEK293_edited_rep_1 838_A6_hg19_chr11:77033596-77033618_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536221 840_C11_hg19_chr8:143220958-143220980_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654090 840_C11_hg19_chr8:143220958-143220980_HEK293_edited_rep_1 840_C11_hg19_chr8:143220958-143220980_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536222 840_B11_hg19_chr16:2900150-2900172_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654095 840_B11_hg19_chr16:2900150-2900172_HEK293_edited_rep_1 840_B11_hg19_chr16:2900150-2900172_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536223 840_A12_hg19_chr22:20167209-20167231_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654093 840_A12_hg19_chr22:20167209-20167231_HEK293_edited_rep_1 840_A12_hg19_chr22:20167209-20167231_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536224 840_H11_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654094 840_H11_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 840_H11_hg19_chr19:55627117-55627139_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536225 840_G11_hg19_chr1:26373692-26373714_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654096 840_G11_hg19_chr1:26373692-26373714_HEK293_edited_rep_1 840_G11_hg19_chr1:26373692-26373714_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536226 839_F3_hg19_chr5:176638327-176638349_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654097 839_F3_hg19_chr5:176638327-176638349_HEK293_edited_rep_1 839_F3_hg19_chr5:176638327-176638349_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536227 831_D5_hg38_chr16:68771133-68771155_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654098 831_D5_hg38_chr16:68771133-68771155_HEK293_edited_rep_1 831_D5_hg38_chr16:68771133-68771155_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536228 831_E5_hg38_chr16:77514936-77514958_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654099 831_E5_hg38_chr16:77514936-77514958_HEK293_edited_rep_1 831_E5_hg38_chr16:77514936-77514958_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536229 831_F5_hg38_chr16:82514938-82514960_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654102 831_F5_hg38_chr16:82514938-82514960_HEK293_edited_rep_1 831_F5_hg38_chr16:82514938-82514960_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536230 831_G5_hg38_chr16:88379544-88379566_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654101 831_G5_hg38_chr16:88379544-88379566_HEK293_edited_rep_1 831_G5_hg38_chr16:88379544-88379566_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536231 831_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654100 831_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 831_H5_hg38_chr6:43769554-43769576_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536232 831_A6_hg38_chr16:89613425-89613447_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654103 831_A6_hg38_chr16:89613425-89613447_HEK293_edited_rep_1 831_A6_hg38_chr16:89613425-89613447_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536233 831_B6_hg38_chr17:3927116-3927138_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654104 831_B6_hg38_chr17:3927116-3927138_HEK293_edited_rep_1 831_B6_hg38_chr17:3927116-3927138_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536234 831_C6_hg38_chr17:4941255-4941277_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654106 831_C6_hg38_chr17:4941255-4941277_HEK293_edited_rep_1 831_C6_hg38_chr17:4941255-4941277_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536235 831_D6_hg38_chr17:6766097-6766119_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654105 831_D6_hg38_chr17:6766097-6766119_HEK293_edited_rep_1 831_D6_hg38_chr17:6766097-6766119_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536236 831_E6_hg38_chr17:21217090-21217112_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654108 831_E6_hg38_chr17:21217090-21217112_HEK293_edited_rep_1 831_E6_hg38_chr17:21217090-21217112_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536237 1734_C5_hg38_chr18:50022998-50023020_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654107 1734_C5_hg38_chr18:50022998-50023020_HEK293_control_rep_1 1734_C5_hg38_chr18:50022998-50023020_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536238 840_C2_hg19_chr2:241136787-241136809_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654110 840_C2_hg19_chr2:241136787-241136809_HEK293_edited_rep_1 840_C2_hg19_chr2:241136787-241136809_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536239 1734_H3_hg38_chr20:43709923-43709945_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654109 1734_H3_hg38_chr20:43709923-43709945_HEK293_control_rep_1 1734_H3_hg38_chr20:43709923-43709945_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536240 832_D1_hg38_chr19:33264493-33264515_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654113 832_D1_hg38_chr19:33264493-33264515_HEK293_edited_rep_1 832_D1_hg38_chr19:33264493-33264515_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536241 1734_A4_hg38_chr15:88631849-88631871_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654111 1734_A4_hg38_chr15:88631849-88631871_HEK293_control_rep_1 1734_A4_hg38_chr15:88631849-88631871_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536242 832_A4_hg38_chr2:66333949-66333971_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654114 832_A4_hg38_chr2:66333949-66333971_HEK293_edited_rep_1 832_A4_hg38_chr2:66333949-66333971_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536243 840_A11_hg19_chr19:17279586-17279608_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654112 840_A11_hg19_chr19:17279586-17279608_HEK293_edited_rep_1 840_A11_hg19_chr19:17279586-17279608_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536244 837_C3_hg19_chr19:40898615-40898637_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654115 837_C3_hg19_chr19:40898615-40898637_HEK293_control_rep_1 837_C3_hg19_chr19:40898615-40898637_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536245 837_B3_hg19_chr20:31098410-31098432_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654117 837_B3_hg19_chr20:31098410-31098432_HEK293_control_rep_1 837_B3_hg19_chr20:31098410-31098432_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536246 837_E2_hg19_chr2:45523611-45523633_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654116 837_E2_hg19_chr2:45523611-45523633_HEK293_control_rep_1 837_E2_hg19_chr2:45523611-45523633_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536247 837_D2_hg19_chr11:132300785-132300807_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654118 837_D2_hg19_chr11:132300785-132300807_HEK293_control_rep_1 837_D2_hg19_chr11:132300785-132300807_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536248 837_C2_hg19_chr2:241136787-241136809_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654119 837_C2_hg19_chr2:241136787-241136809_HEK293_control_rep_1 837_C2_hg19_chr2:241136787-241136809_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536249 837_B2_hg19_chr12:112064595-112064617_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654120 837_B2_hg19_chr12:112064595-112064617_HEK293_control_rep_1 837_B2_hg19_chr12:112064595-112064617_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536250 837_A3_hg19_chr10:128585309-128585331_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654122 837_A3_hg19_chr10:128585309-128585331_HEK293_control_rep_1 837_A3_hg19_chr10:128585309-128585331_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536251 837_H2_hg19_chr5:54187613-54187635_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654123 837_H2_hg19_chr5:54187613-54187635_HEK293_control_rep_1 837_H2_hg19_chr5:54187613-54187635_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536252 837_G2_hg19_chrX:17811010-17811032_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654121 837_G2_hg19_chrX:17811010-17811032_HEK293_control_rep_1 837_G2_hg19_chrX:17811010-17811032_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536253 837_F2_hg19_chr11:4621708-4621730_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654126 837_F2_hg19_chr11:4621708-4621730_HEK293_control_rep_1 837_F2_hg19_chr11:4621708-4621730_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536254 833_D5_hg38_chr3:36317425-36317447_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654124 833_D5_hg38_chr3:36317425-36317447_HEK293_edited_rep_1 833_D5_hg38_chr3:36317425-36317447_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536255 833_E5_hg38_chr3:38275174-38275196_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654128 833_E5_hg38_chr3:38275174-38275196_HEK293_edited_rep_1 833_E5_hg38_chr3:38275174-38275196_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536256 833_B5_hg38_chr3:21526829-21526851_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654129 833_B5_hg38_chr3:21526829-21526851_HEK293_edited_rep_1 833_B5_hg38_chr3:21526829-21526851_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536257 833_C5_hg38_chr3:36251548-36251570_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654125 833_C5_hg38_chr3:36251548-36251570_HEK293_edited_rep_1 833_C5_hg38_chr3:36251548-36251570_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536258 835_C2_hg19_chr9:34350221-34350243_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654127 835_C2_hg19_chr9:34350221-34350243_HEK293_control_rep_1 835_C2_hg19_chr9:34350221-34350243_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536259 835_B2_hg19_chr8:141962476-141962498_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654131 835_B2_hg19_chr8:141962476-141962498_HEK293_control_rep_1 835_B2_hg19_chr8:141962476-141962498_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536260 833_F5_hg38_chr3:39363414-39363436_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654130 833_F5_hg38_chr3:39363414-39363436_HEK293_edited_rep_1 833_F5_hg38_chr3:39363414-39363436_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536261 833_G5_hg38_chr3:45888248-45888270_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the VEGFA locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654132 833_G5_hg38_chr3:45888248-45888270_HEK293_edited_rep_1 833_G5_hg38_chr3:45888248-45888270_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536262 835_G1_hg19_chr13:106612912-106612934_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654134 835_G1_hg19_chr13:106612912-106612934_HEK293_control_rep_1 835_G1_hg19_chr13:106612912-106612934_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536263 835_F1_hg19_chr2:111977953-111977975_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654133 835_F1_hg19_chr2:111977953-111977975_HEK293_control_rep_1 835_F1_hg19_chr2:111977953-111977975_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536264 835_A2_hg19_chrX:23229670-23229692_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654135 835_A2_hg19_chrX:23229670-23229692_HEK293_control_rep_1 835_A2_hg19_chrX:23229670-23229692_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536265 835_H1_hg19_chr20:60730921-60730943_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654136 835_H1_hg19_chr20:60730921-60730943_HEK293_control_rep_1 835_H1_hg19_chr20:60730921-60730943_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536266 835_C1_hg19_chr11:62101680-62101702_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654137 835_C1_hg19_chr11:62101680-62101702_HEK293_control_rep_1 835_C1_hg19_chr11:62101680-62101702_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536267 835_B1_hg19_chr16:10133368-10133390_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654139 835_B1_hg19_chr16:10133368-10133390_HEK293_control_rep_1 835_B1_hg19_chr16:10133368-10133390_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536268 835_E1_hg19_chr3:10127644-10127666_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654138 835_E1_hg19_chr3:10127644-10127666_HEK293_control_rep_1 835_E1_hg19_chr3:10127644-10127666_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536269 835_D1_hg19_chr11:120979641-120979663_HEK293_control_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654141 835_D1_hg19_chr11:120979641-120979663_HEK293_control_rep_1 835_D1_hg19_chr11:120979641-120979663_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536270 839_C10_hg19_chr5:176741530-176741552_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654140 839_C10_hg19_chr5:176741530-176741552_HEK293_edited_rep_1 839_C10_hg19_chr5:176741530-176741552_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536271 839_B10_hg19_chr11:11302409-11302431_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654144 839_B10_hg19_chr11:11302409-11302431_HEK293_edited_rep_1 839_B10_hg19_chr11:11302409-11302431_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536272 839_A10_hg19_chr1:221108290-221108312_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654146 839_A10_hg19_chr1:221108290-221108312_HEK293_edited_rep_1 839_A10_hg19_chr1:221108290-221108312_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536273 839_H9_hg19_chr19:8957091-8957113_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654142 839_H9_hg19_chr19:8957091-8957113_HEK293_edited_rep_1 839_H9_hg19_chr19:8957091-8957113_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536274 839_G9_hg19_chr8:57454928-57454950_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654152 839_G9_hg19_chr8:57454928-57454950_HEK293_edited_rep_1 839_G9_hg19_chr8:57454928-57454950_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536275 839_F9_hg19_chr19:51871850-51871872_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654149 839_F9_hg19_chr19:51871850-51871872_HEK293_edited_rep_1 839_F9_hg19_chr19:51871850-51871872_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536276 839_E9_hg19_chr22:37948800-37948822_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654150 839_E9_hg19_chr22:37948800-37948822_HEK293_edited_rep_1 839_E9_hg19_chr22:37948800-37948822_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536277 839_D9_hg19_chr12:133249100-133249122_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654143 839_D9_hg19_chr12:133249100-133249122_HEK293_edited_rep_1 839_D9_hg19_chr12:133249100-133249122_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536278 1734_F3_hg38_chr17:20447161-20447183_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654147 1734_F3_hg38_chr17:20447161-20447183_HEK293_control_rep_1 1734_F3_hg38_chr17:20447161-20447183_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536279 1734_G3_hg38_chr8:142139597-142139619_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654154 1734_G3_hg38_chr8:142139597-142139619_HEK293_control_rep_1 1734_G3_hg38_chr8:142139597-142139619_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536280 1734_D3_hg38_chr16:33884563-33884585_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654145 1734_D3_hg38_chr16:33884563-33884585_HEK293_control_rep_1 1734_D3_hg38_chr16:33884563-33884585_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536281 1734_A3_hg38_chr11:78787107-78787129_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654156 1734_A3_hg38_chr11:78787107-78787129_HEK293_control_rep_1 1734_A3_hg38_chr11:78787107-78787129_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536282 1734_B3_hg38_chr7:2104508-2104530_HEK293_control_rep_1 SRP105100 PRJNA329375 SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654151 1734_B3_hg38_chr7:2104508-2104530_HEK293_control_rep_1 1734_B3_hg38_chr7:2104508-2104530_HEK293_control_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536283 840_H1_hg19_chr8:17484307-17484329_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654153 840_H1_hg19_chr8:17484307-17484329_HEK293_edited_rep_1 840_H1_hg19_chr8:17484307-17484329_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536284 839_E10_hg19_chr20:44972632-44972654_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654148 839_E10_hg19_chr20:44972632-44972654_HEK293_edited_rep_1 839_E10_hg19_chr20:44972632-44972654_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4536285 839_D10_hg19_chr10:120117150-120117172_HEK293_edited_rep_1 SRP105100 PRJNA329375 sgRNPs were targeted to the AAVS1 locus. On- and off-targets were measured after ~2-3 days. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage SRS3654155 839_D10_hg19_chr10:120117150-120117172_HEK293_edited_rep_1 839_D10_hg19_chr10:120117150-120117172_HEK293_edited_rep_1 AMPLICON GENOMIC PCR Illumina MiSeq