SRX4555975 MG9 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672974 PAN8906 MG9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4555976 MG10 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672975 PAN8907W MG10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4555977 MG7 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672977 PAN8420 MG7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4555978 MG8 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672976 PAN8625 MG8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4555979 MG5 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672979 NSSS11 MG5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX4555980 MG6 SRP158008 bp0 Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform. SRS3672980 PAN8816 MG6 AMPLICON METAGENOMIC PCR Illumina MiSeq