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    <TITLE>GSM3370061: Col-0 Control AM Rep 1; Arabidopsis thaliana; RNA-Seq</TITLE>
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    <TITLE>GSM3370062: Col-0 Control AM Rep 2; Arabidopsis thaliana; RNA-Seq</TITLE>
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      <PRIMARY_ID>SRX4629451</PRIMARY_ID>
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    <TITLE>GSM3370063: Col-0 Control AM Rep 3; Arabidopsis thaliana; RNA-Seq</TITLE>
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        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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      <PRIMARY_ID>SRX4629452</PRIMARY_ID>
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    <TITLE>GSM3370064: Col-0 Control AM Rep 4; Arabidopsis thaliana; RNA-Seq</TITLE>
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          <SINGLE/>
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      <PRIMARY_ID>SRX4629453</PRIMARY_ID>
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    <TITLE>GSM3370065: Col-0 Control PM Rep 1; Arabidopsis thaliana; RNA-Seq</TITLE>
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          <SINGLE/>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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    <TITLE>GSM3370066: Col-0 Control PM Rep 2; Arabidopsis thaliana; RNA-Seq</TITLE>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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    <TITLE>GSM3370069: Col-0 Heat AM Rep 1; Arabidopsis thaliana; RNA-Seq</TITLE>
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          <EXTERNAL_ID namespace="GEO">GSM3370069</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370069</ID>
          <LABEL>GSM3370069</LABEL>
        </XREF_LINK>
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    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370069</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3370070" accession="SRX4629458">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX4629458</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370070</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370070: Col-0 Heat AM Rep 2; Arabidopsis thaliana; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP159259" refname="GSE119330">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS3730419">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS3730419</PRIMARY_ID>
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      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
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    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370070</ID>
          <LABEL>GSM3370070</LABEL>
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    </EXPERIMENT_LINKS>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370070</VALUE>
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    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3370071" accession="SRX4629459">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX4629459</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370071</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370071: Col-0 Heat AM Rep 3; Arabidopsis thaliana; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP159259" refname="GSE119330">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS3730420">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS3730420</PRIMARY_ID>
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      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370071</ID>
          <LABEL>GSM3370071</LABEL>
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    </EXPERIMENT_LINKS>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370071</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3370072" accession="SRX4629460">
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      <PRIMARY_ID>SRX4629460</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370072</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370072: Col-0 Heat AM Rep 4; Arabidopsis thaliana; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP159259" refname="GSE119330">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS3730421">
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          <PRIMARY_ID>SRS3730421</PRIMARY_ID>
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        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
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    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
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      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370072</ID>
          <LABEL>GSM3370072</LABEL>
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    </EXPERIMENT_LINKS>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
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  <EXPERIMENT alias="GSM3370073" accession="SRX4629461">
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      <PRIMARY_ID>SRX4629461</PRIMARY_ID>
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    </IDENTIFIERS>
    <TITLE>GSM3370073: Col-0 Heat PM Rep 1; Arabidopsis thaliana; RNA-Seq</TITLE>
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      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <SAMPLE_DESCRIPTOR accession="SRS3730422">
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          <PRIMARY_ID>SRS3730422</PRIMARY_ID>
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        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
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    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370073</ID>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
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  <EXPERIMENT alias="GSM3370074" accession="SRX4629462">
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      <PRIMARY_ID>SRX4629462</PRIMARY_ID>
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    <TITLE>GSM3370074: Col-0 Heat PM Rep 2; Arabidopsis thaliana; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <SAMPLE_DESCRIPTOR accession="SRS3730423">
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          <PRIMARY_ID>SRS3730423</PRIMARY_ID>
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        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
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      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
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      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370074</ID>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
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    </EXPERIMENT_ATTRIBUTES>
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  <EXPERIMENT alias="GSM3370075" accession="SRX4629463">
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      <PRIMARY_ID>SRX4629463</PRIMARY_ID>
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    <TITLE>GSM3370075: Col-0 Heat PM Rep 3; Arabidopsis thaliana; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <SAMPLE_DESCRIPTOR accession="SRS3730424">
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          <PRIMARY_ID>SRS3730424</PRIMARY_ID>
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          <SINGLE/>
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        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
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      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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    </PLATFORM>
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      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370075</ID>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
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    </EXPERIMENT_ATTRIBUTES>
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  <EXPERIMENT alias="GSM3370076" accession="SRX4629464">
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      <PRIMARY_ID>SRX4629464</PRIMARY_ID>
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    <TITLE>GSM3370076: Col-0 Heat PM Rep 4; Arabidopsis thaliana; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <SAMPLE_DESCRIPTOR accession="SRS3730425">
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          <PRIMARY_ID>SRS3730425</PRIMARY_ID>
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          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
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    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370076</ID>
          <LABEL>GSM3370076</LABEL>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
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    </EXPERIMENT_ATTRIBUTES>
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  <EXPERIMENT alias="GSM3370077" accession="SRX4629465">
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      <PRIMARY_ID>SRX4629465</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370077</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370077: Col-0 LL_LDHH Control AM; Arabidopsis thaliana; RNA-Seq</TITLE>
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      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
      </IDENTIFIERS>
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      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS3730426">
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          <PRIMARY_ID>SRS3730426</PRIMARY_ID>
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        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
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      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370077</ID>
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    </EXPERIMENT_LINKS>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370077</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3370078" accession="SRX4629466">
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      <PRIMARY_ID>SRX4629466</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370078</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370078: Col-0 LL_LDHH Control PM; Arabidopsis thaliana; RNA-Seq</TITLE>
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      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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          <PRIMARY_ID>SRS3730427</PRIMARY_ID>
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        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
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          <DB>gds</DB>
          <ID>303370078</ID>
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        <TAG>GEO Accession</TAG>
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    </EXPERIMENT_ATTRIBUTES>
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      <PRIMARY_ID>SRX4629467</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370079</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370079: Col-0 LL_LDHH Heat AM; Arabidopsis thaliana; RNA-Seq</TITLE>
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      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
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      <SAMPLE_DESCRIPTOR accession="SRS3730428">
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          <PRIMARY_ID>SRS3730428</PRIMARY_ID>
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      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
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        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
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    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
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          <DB>gds</DB>
          <ID>303370079</ID>
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      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370079</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3370080" accession="SRX4629468">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX4629468</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3370080</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3370080: Col-0 LL_LDHH Heat PM; Arabidopsis thaliana; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP159259" refname="GSE119330">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP159259</PRIMARY_ID>
      </IDENTIFIERS>
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      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS3730429">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS3730429</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM3370080</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Aerial tissue was harvested and frozen in liquid nitrogen prior to RNA extraction. Purified total RNA was extracted from 4 replicates of mock treated and heat shocked plants using Qiagen RNeasy Plant Minikits, following manufacturer protocols. Samples were diluted in order to acquire two micrograms of RNA in preparation for mRNA isolation. mRNA was separated using the NEBNext Poly(A) Magnetic mRNA isolation kit (New England Biolabs). Before library preparation, mRNA samples were heated to 95°C for 15 minutes to achieve 150-200 base pair fragment sizes. NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was then used to generate libraries for sequencing. cDNA was synthesized with random hexamers.  After performing end repair and adaptor ligation steps, Agencourt AMPure XP beads (Beckman Coulter) were used following recommended ratios in order to isolate 150-200 bp fragments and remove adaptors. After, end repair and adaptor ligation size selection was performed with AMPure beads to isolate 150-200bp fragments and removed adaptors. PCR library enrichment for fifteen cycles was performed. The libraries were run on Agilent Bioanalyzer high sensitivity DNA chip after a 1:4 (or 1:10 if the concentration is too high) dilution. NEBNext Library Quant Kit for Illumina was used to measure library quantity and quality. Finally, the libraries were diluted to 10 nmol/ul concentrations before sequencing.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303370080</ID>
          <LABEL>GSM3370080</LABEL>
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    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM3370080</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
