SRX4633087 GSM3371777 SRP159428 SRS3733826 GSM3371777 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371777 GSM3371777 GEO Accession GSM3371777 SRX4633088 GSM3371778 SRP159428 SRS3733807 GSM3371778 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371778 GSM3371778 GEO Accession GSM3371778 SRX4633089 GSM3371779 SRP159428 SRS3733808 GSM3371779 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371779 GSM3371779 GEO Accession GSM3371779 SRX4633090 GSM3371780 SRP159428 SRS3733809 GSM3371780 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371780 GSM3371780 GEO Accession GSM3371780 SRX4633091 GSM3371781 SRP159428 SRS3733810 GSM3371781 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371781 GSM3371781 GEO Accession GSM3371781 SRX4633092 GSM3371782 SRP159428 SRS3733811 GSM3371782 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371782 GSM3371782 GEO Accession GSM3371782 SRX4633093 GSM3371783 SRP159428 SRS3733812 GSM3371783 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371783 GSM3371783 GEO Accession GSM3371783 SRX4633094 GSM3371784 SRP159428 SRS3733813 GSM3371784 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371784 GSM3371784 GEO Accession GSM3371784 SRX4633095 GSM3371785 SRP159428 SRS3733814 GSM3371785 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371785 GSM3371785 GEO Accession GSM3371785 SRX4633096 GSM3371786 SRP159428 SRS3733815 GSM3371786 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371786 GSM3371786 GEO Accession GSM3371786 SRX4633097 GSM3371787 SRP159428 SRS3733816 GSM3371787 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371787 GSM3371787 GEO Accession GSM3371787 SRX4633098 GSM3371788 SRP159428 SRS3733817 GSM3371788 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371788 GSM3371788 GEO Accession GSM3371788 SRX4633099 GSM3371789 SRP159428 SRS3733818 GSM3371789 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371789 GSM3371789 GEO Accession GSM3371789 SRX4633100 GSM3371790 SRP159428 SRS3733819 GSM3371790 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371790 GSM3371790 GEO Accession GSM3371790 SRX4633101 GSM3371791 SRP159428 SRS3733820 GSM3371791 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371791 GSM3371791 GEO Accession GSM3371791 SRX4633102 GSM3371792 SRP159428 SRS3733821 GSM3371792 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371792 GSM3371792 GEO Accession GSM3371792 SRX4633103 GSM3371793 SRP159428 SRS3733822 GSM3371793 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371793 GSM3371793 GEO Accession GSM3371793 SRX4633104 GSM3371794 SRP159428 SRS3733823 GSM3371794 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371794 GSM3371794 GEO Accession GSM3371794 SRX4633105 GSM3371795 SRP159428 SRS3733824 GSM3371795 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371795 GSM3371795 GEO Accession GSM3371795 SRX4633106 GSM3371796 SRP159428 SRS3733825 GSM3371796 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371796 GSM3371796 GEO Accession GSM3371796 SRX4633107 GSM3371797 SRP159428 SRS3733827 GSM3371797 RNA-Seq TRANSCRIPTOMIC cDNA For Ribo-seq, sorted erythroblasts were washed twice with cold phosphate-buffered saline and then treated with cycloheximde (100 ug/ml) at 37℃ for 5 min, followed by isolation of ribosome protected fragments (RPFs) using ARTseq-Ribosome Profiling Kit (Illumina). For mRNA-seq, total RNAs were extracted by RNeasy Plus kit (Qiagen) and polyA RNAs were isolated using an Oligotex mRNA kit (Qiagen). Ribo-seq cDNA libraries were prepared as described (Ingolia et al. 2009, Ingolia, Lareau, and Weissman 2011). Briefly, RPFs were dephosphorylated and linkers were ligated using T4 RNA ligase. RNA samples were then reverse transcribed, circularized and PCR amplified for 12 cycles. PCR products were subjected to gel purification before sequencing. mRNA-seq cDNA libraries were prepared by the MIT BioMicro Center. Illumina HiSeq 2000 gds 303371797 GSM3371797 GEO Accession GSM3371797