SRX4636261 OVCAR-3 DRUG_2 SRP159483 PRJNA477252 The whole transcriptome library for sequencing is made using Sense Total RNA-seq Library prep kit . This protocol maintains the strand specificity to allow the mapping of the genes to the corresponding strand. The workflow involves depletion of ribosomal RNA, including the mitochondrial fraction, followed by the random hybridization of Starter/Stopper heterodimers containing Illumina-compatible linkersequences to the RNA template. Reverse transcription starts from the Starter oligo and ends at Stopper oligo where the cDNA is ligated. Then, the second strand is generated followed by PCR amplification where P5 and P7 adapters are added which is required for cluster generation SRS3736031 SAMN09945382 OVCAR-3 DRUG_2 RNA-Seq TRANSCRIPTOMIC other Illumina HiSeq 2500 SRX4636262 OVCAR-3 DRUG_1 SRP159483 PRJNA477252 The whole transcriptome library for sequencing is made using Sense Total RNA-seq Library prep kit . This protocol maintains the strand specificity to allow the mapping of the genes to the corresponding strand. The workflow involves depletion of ribosomal RNA, including the mitochondrial fraction, followed by the random hybridization of Starter/Stopper heterodimers containing Illumina-compatible linkersequences to the RNA template. Reverse transcription starts from the Starter oligo and ends at Stopper oligo where the cDNA is ligated. Then, the second strand is generated followed by PCR amplification where P5 and P7 adapters are added which is required for cluster generation SRS3736032 SAMN09945381 OVCAR-3 DRUG_1 RNA-Seq TRANSCRIPTOMIC other Illumina HiSeq 2500 SRX4636263 OVCAR-3 DRUG_3 SRP159483 PRJNA477252 The whole transcriptome library for sequencing is made using Sense Total RNA-seq Library prep kit . This protocol maintains the strand specificity to allow the mapping of the genes to the corresponding strand. The workflow involves depletion of ribosomal RNA, including the mitochondrial fraction, followed by the random hybridization of Starter/Stopper heterodimers containing Illumina-compatible linkersequences to the RNA template. Reverse transcription starts from the Starter oligo and ends at Stopper oligo where the cDNA is ligated. Then, the second strand is generated followed by PCR amplification where P5 and P7 adapters are added which is required for cluster generation SRS3736033 SAMN09945383 OVCAR-3 DRUG_3 RNA-Seq TRANSCRIPTOMIC other Illumina HiSeq 2500