SRX4638190 Y5 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-833R. SRS3736857 Y5 Y5 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638191 Y4 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-832R. SRS3736858 Y4 Y4 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638192 Y3 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-831R. SRS3736859 Y3 Y3 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638193 Y2 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-830R. SRS3736860 Y2 Y2 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638194 Y1 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-829R. SRS3736861 Y1 Y1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638195 H6 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-828R. SRS3736862 H6 H6 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638196 H5 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-827R. SRS3736863 H5 H5 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638197 H4 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-826R. SRS3736864 H4 H4 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638198 Y6 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-834R. SRS3736868 Y6 Y6 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638199 DH1 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-806R. SRS3736865 DH1 DH1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638200 DH2 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-807R. SRS3736866 DH2 DH2 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638201 DH3 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-808R. SRS3736867 DH3 DH3 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638202 DH4 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-809R. SRS3736875 DH4 DH4 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638203 DH5 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-810R. SRS3736869 DH5 DH5 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638204 DH6 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-811R. SRS3736870 DH6 DH6 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638205 DL1 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-812R. SRS3736871 DL1 DL1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638206 DL2 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-813R. SRS3736872 DL2 DL2 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638207 DL3 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-814R. SRS3736873 DL3 DL3 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638208 DL4 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-815R. SRS3736874 DL4 DL4 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638209 H2 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-824R. SRS3736885 H2 H2 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638210 H3 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-825R. SRS3736876 H3 H3 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638211 G1 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-818R. SRS3736877 G1 G1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638212 G3 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-819R. SRS3736878 G3 G3 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638213 DL5 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-816R. SRS3736879 DL5 DL5 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638214 DL6 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-817R. SRS3736880 DL6 DL6 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638215 G6 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-822R. SRS3736881 G6 G6 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638216 H1 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-823R. SRS3736882 H1 H1 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638217 G4 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-820R. SRS3736883 G4 G4 AMPLICON GENOMIC PCR Illumina MiSeq SRX4638218 G5 SRP159535 bp0 The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-821R. SRS3736884 G5 G5 AMPLICON GENOMIC PCR Illumina MiSeq