SRX4647071 GSM3377785 SRP159824 SRS3745101 GSM3377785 RNA-Seq TRANSCRIPTOMIC cDNA Ileal tissues taken from fed and fasted mice were lysed with lysis buffer and treated with nuclease. Ribosome-protected fragments (RPF) were separated with a sucrose-cushion. Footprint fragments were purified and used to generate deep sequencing libraries. After rRNA depletion DNA was PCR amplified with Illumina primers for 10 cycles and library fragments of ~175 bp were band isolated from a polyacrylamide TBE-urea gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols. Illumina HiSeq 4000 gds 303377785 GSM3377785 GEO Accession GSM3377785 SRX4647072 GSM3377786 SRP159824 SRS3745102 GSM3377786 RNA-Seq TRANSCRIPTOMIC cDNA Ileal tissues taken from fed and fasted mice were lysed with lysis buffer and treated with nuclease. Ribosome-protected fragments (RPF) were separated with a sucrose-cushion. Footprint fragments were purified and used to generate deep sequencing libraries. After rRNA depletion DNA was PCR amplified with Illumina primers for 10 cycles and library fragments of ~175 bp were band isolated from a polyacrylamide TBE-urea gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols. Illumina HiSeq 4000 gds 303377786 GSM3377786 GEO Accession GSM3377786