SRX4712742 PvRoot SRP162055 bp0 Total RNA was extracted from roots of mature plants using a CTAB based method. mRNA was selected from 16 g total RNA using Dynabeads Oligo (dT)25 (ThermoFisher Scientific) yielding 119 ng mRNA. The mRNA was converted to cDNA using the Oxford Nanopore cDNA-PCR Sequencing Kit (SQK-PCS108), and sequenced on the GridIon SRS3798510 PvRoot PvRoot RNA-Seq TRANSCRIPTOMIC RT-PCR GridION