SRX4712738 GSM3394913 SRP162054 SRS3798506 GSM3394913 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394913 GSM3394913 GEO Accession GSM3394913 SRX4712736 GSM3394911 SRP162054 SRS3798504 GSM3394911 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394911 GSM3394911 GEO Accession GSM3394911 SRX4712740 GSM3394915 SRP162054 SRS3798508 GSM3394915 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394915 GSM3394915 GEO Accession GSM3394915 SRX4712741 GSM3394916 SRP162054 SRS3798509 GSM3394916 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394916 GSM3394916 GEO Accession GSM3394916 SRX4712739 GSM3394914 SRP162054 SRS3798507 GSM3394914 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394914 GSM3394914 GEO Accession GSM3394914 SRX4712737 GSM3394912 SRP162054 SRS3798505 GSM3394912 miRNA-Seq TRANSCRIPTOMIC size fractionation Prefrontal cortex tissue including medial orbital, prelimbic, infralimbic, and cingulate 1 cortex was collected using 1 and 2 mm round tissue punches while sectioning brains on a cryostat collecting tissue between 2.46 mm and 1.1 mm Bregma. Synaptosomes were prepared pooling PFC punches from n=15 mice as previously described (Filiou et al., 2010; Lopes et al., 2017) working RNase-free. Total RNA from was purified using the miRNeasy micro kit (QIAGEN) following the manufacturer's instructions. Small RNA-Seq libraries were prepared from 50 ng synaptosomal RNA using the Illumina TruSeq Small RNA Library Preparation Kit using Superscript III (Invitrogen), 12 cycles of PCR and size-selection on DNA-PAGE. Synthetic spike-in miRNAs (Williams et al., 2013) were used at a concentration of 0.005 fmol in library preps but not considered for analysis. Illumina MiSeq gds 303394912 GSM3394912 GEO Accession GSM3394912