Targeted RNA-seq of Patient A

SRX4717690 MRD13-1 Targeted RNA-seq of Patient A SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted NPM1, ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803260 Patient A MRD13-1 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717691 MRD13-2 Targeted RNA-seq of Patient B SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted NPM1, ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803261 Patient B MRD13-2 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717692 MRD10-1 Targeted RNA-seq of Patient D SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803262 Patient D MRD10-1 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717693 MRD13-5 Targeted RNA-seq of Patient E SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803263 Patient E MRD13-5 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717694 MRD13-7 Targeted RNA-seq of Patient F SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803268 Patient F MRD13-7 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717695 MRD13-6 Targeted RNA-seq of Patient G SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803264 Patient G MRD13-6 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717696 MRD19-1 Targeted RNA-seq of Patient H SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803265 Patient H MRD19-1 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717697 MRD19-2 Targeted RNA-seq of Patient I SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803266 Patient I MRD19-2 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX4717698 MRD20-1 Targeted RNA-seq of Patient J SRP126362 PRJNA421563 Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction. The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1. SRS3803267 Patient J MRD20-1 AMPLICON TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500