SRX4727767 GSM3398380 SRP162360 SRS3811254 GSM3398380 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398380 GSM3398380 GEO Accession GSM3398380 SRX4727768 GSM3398381 SRP162360 SRS3811255 GSM3398381 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398381 GSM3398381 GEO Accession GSM3398381 SRX4727769 GSM3398382 SRP162360 SRS3811256 GSM3398382 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398382 GSM3398382 GEO Accession GSM3398382 SRX4727770 GSM3398383 SRP162360 SRS3811257 GSM3398383 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398383 GSM3398383 GEO Accession GSM3398383 SRX4727771 GSM3398384 SRP162360 SRS3811258 GSM3398384 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398384 GSM3398384 GEO Accession GSM3398384 SRX4727772 GSM3398385 SRP162360 SRS3811259 GSM3398385 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398385 GSM3398385 GEO Accession GSM3398385 SRX4727773 GSM3398386 SRP162360 SRS3811260 GSM3398386 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398386 GSM3398386 GEO Accession GSM3398386 SRX4727774 GSM3398387 SRP162360 SRS3811261 GSM3398387 RNA-Seq TRANSCRIPTOMIC cDNA Deletion/KO and wild-type embryos at embryonic day 14 were dissected, their neural tubes were dissociated by collagenase type 2 to yield single cells, and mNPCs isolated by FACS using NeuroFluor CDr3 (STEMCELL Technologies, #01800). Isolated mNPCs were analyzed or cultured in differentiation media (NeuroCult differentiation, STEMCELL Technologies, #05704) for further analyses total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.) Illumina NovaSeq 6000 gds 303398387 GSM3398387 GEO Accession GSM3398387