SRX4823819 GSM3425411 SRP164901 SRS3902826 GSM3425411 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425411 GSM3425411 GEO Accession GSM3425411 SRX4823820 GSM3425412 SRP164901 SRS3897647 GSM3425412 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425412 GSM3425412 GEO Accession GSM3425412 SRX4823821 GSM3425413 SRP164901 SRS3897648 GSM3425413 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425413 GSM3425413 GEO Accession GSM3425413 SRX4823822 GSM3425414 SRP164901 SRS3897649 GSM3425414 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425414 GSM3425414 GEO Accession GSM3425414 SRX4823823 GSM3425415 SRP164901 SRS3897650 GSM3425415 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425415 GSM3425415 GEO Accession GSM3425415 SRX4823824 GSM3425416 SRP164901 SRS3897651 GSM3425416 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425416 GSM3425416 GEO Accession GSM3425416 SRX4823825 GSM3425417 SRP164901 SRS3897652 GSM3425417 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425417 GSM3425417 GEO Accession GSM3425417 SRX4823826 GSM3425418 SRP164901 SRS3897653 GSM3425418 MNase-Seq GENOMIC MNase Extracts for MNase were resuspended in 1M sorbitol and digested 1 hour with 4.5 mg of Zymoliase 20T (AmsBio 120491-1). Samples were then washed with 1 M sorbitol and 1M sorbitol PMSF 0.1mM, suspended gently in solution II (20mM Tris HCl 2mM EDTA 0.15M NaCl 0.1mM PMSF 0.2 % Triton), and treated 30 minutes with different concentrations of MNase (SIGMA N3755). The reaction was then stopped adding SDS (0.4%) and EDTA (8.5mM). Samples were incubated for 90 minutes at 37ºC with proteinase K and overnight at 65ºC for reversal crosslinking. DNA was extracted from samples using a standard phenol-chloroform extraction, treated with RNase A and loaded in a 1% agarose gel to check MNase digestion. MNase digestions enriched in mononucleosomes were loaded in a 1% agarose gel, and the DNA corresponding to mononucleosomes was purified with a DNA purification kit (Qiagen). The DNA size and quality was confirmed by an electropherogram analysis. Libraries were generated using the Ion Plus Fragment Library Kit (4471252, Thermofisher) with 100ng input DNA. DNA fragments were size-selected around 230 bp after ligation of adapters. Emulsion PCR allow enrich monocloning libraries Ion Torrent PGM gds 303425418 GSM3425418 GEO Accession GSM3425418