GSM3431013: XO_ESC-1_S5_L001; Mus musculus; RNA-Seq

SRX4891680 GSM3431013 GSM3431013: XO_ESC-1_S5_L001; Mus musculus; RNA-Seq SRP165841 SRS3938775 GSM3431013 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431013 GSM3431013 GEO Accession GSM3431013 SRX4891681 GSM3431014 GSM3431014: XO_ESC-2_S6_L001; Mus musculus; RNA-Seq SRP165841 SRS3938776 GSM3431014 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431014 GSM3431014 GEO Accession GSM3431014 SRX4891682 GSM3431015 GSM3431015: XO_IVD_ag3-1_S28_L003; Mus musculus; RNA-Seq SRP165841 SRS3938777 GSM3431015 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431015 GSM3431015 GEO Accession GSM3431015 SRX4891683 GSM3431016 GSM3431016: XO_IVD_ag3-2_S29_L003; Mus musculus; RNA-Seq SRP165841 SRS3938778 GSM3431016 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431016 GSM3431016 GEO Accession GSM3431016 SRX4891684 GSM3431017 GSM3431017: XO_IVD_ag3-3_S30_L003; Mus musculus; RNA-Seq SRP165841 SRS3938779 GSM3431017 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431017 GSM3431017 GEO Accession GSM3431017 SRX4891685 GSM3431018 GSM3431018: XO_IVD_ag5-1_S37_L004; Mus musculus; RNA-Seq SRP165841 SRS3938780 GSM3431018 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431018 GSM3431018 GEO Accession GSM3431018 SRX4891686 GSM3431019 GSM3431019: XO_IVD_ag5-2_S38_L004; Mus musculus; RNA-Seq SRP165841 SRS3938781 GSM3431019 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431019 GSM3431019 GEO Accession GSM3431019 SRX4891687 GSM3431020 GSM3431020: XO_IVD_ag5-3_S39_L004; Mus musculus; RNA-Seq SRP165841 SRS3938782 GSM3431020 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431020 GSM3431020 GEO Accession GSM3431020 SRX4891688 GSM3431021 GSM3431021: XO_IVD_ag7-1_S46_L004; Mus musculus; RNA-Seq SRP165841 SRS3938783 GSM3431021 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431021 GSM3431021 GEO Accession GSM3431021 SRX4891689 GSM3431022 GSM3431022: XO_IVD_ag7-2_S47_L004; Mus musculus; RNA-Seq SRP165841 SRS3938784 GSM3431022 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431022 GSM3431022 GEO Accession GSM3431022 SRX4891690 GSM3431023 GSM3431023: XO_IVD_ag7-3_S48_L004; Mus musculus; RNA-Seq SRP165841 SRS3938785 GSM3431023 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431023 GSM3431023 GEO Accession GSM3431023 SRX4891692 GSM3431024 GSM3431024: XO_d2EpiLC-1_S11_L001; Mus musculus; RNA-Seq SRP165841 SRS3938787 GSM3431024 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431024 GSM3431024 GEO Accession GSM3431024 SRX4891693 GSM3431025 GSM3431025: XO_d2EpiLC-2_S12_L001; Mus musculus; RNA-Seq SRP165841 SRS3938788 GSM3431025 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431025 GSM3431025 GEO Accession GSM3431025 SRX4891694 GSM3431026 GSM3431026: XO_d6PGCLC-1_S19_L002; Mus musculus; RNA-Seq SRP165841 SRS3938789 GSM3431026 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431026 GSM3431026 GEO Accession GSM3431026 SRX4891695 GSM3431027 GSM3431027: XO_d6PGCLC-2_S20_L002; Mus musculus; RNA-Seq SRP165841 SRS3938790 GSM3431027 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431027 GSM3431027 GEO Accession GSM3431027 SRX4891696 GSM3431028 GSM3431028: XO_d6PGCLC-3_S21_L002; Mus musculus; RNA-Seq SRP165841 SRS3938791 GSM3431028 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431028 GSM3431028 GEO Accession GSM3431028 SRX4891697 GSM3431029 GSM3431029: XX_ESC-1_S1_L001; Mus musculus; RNA-Seq SRP165841 SRS3938792 GSM3431029 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431029 GSM3431029 GEO Accession GSM3431029 SRX4891698 GSM3431030 GSM3431030: XX_ESC-2_S2_L001; Mus musculus; RNA-Seq SRP165841 SRS3938793 GSM3431030 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431030 GSM3431030 GEO Accession GSM3431030 SRX4891699 GSM3431031 GSM3431031: XX_IVD_ag3-1_S22_L002; Mus musculus; RNA-Seq SRP165841 SRS3938794 GSM3431031 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431031 GSM3431031 GEO Accession GSM3431031 SRX4891700 GSM3431032 GSM3431032: XX_IVD_ag3-2_S23_L002; Mus musculus; RNA-Seq SRP165841 SRS3938796 GSM3431032 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431032 GSM3431032 GEO Accession GSM3431032 SRX4891701 GSM3431033 GSM3431033: XX_IVD_ag3-3_S24_L002; Mus musculus; RNA-Seq SRP165841 SRS3938797 GSM3431033 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431033 GSM3431033 GEO Accession GSM3431033 SRX4891702 GSM3431034 GSM3431034: XX_IVD_ag5-1_S31_L003; Mus musculus; RNA-Seq SRP165841 SRS3938795 GSM3431034 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431034 GSM3431034 GEO Accession GSM3431034 SRX4891703 GSM3431035 GSM3431035: XX_IVD_ag5-2_S32_L003; Mus musculus; RNA-Seq SRP165841 SRS3938798 GSM3431035 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431035 GSM3431035 GEO Accession GSM3431035 SRX4891704 GSM3431036 GSM3431036: XX_IVD_ag5-3_S33_L003; Mus musculus; RNA-Seq SRP165841 SRS3938799 GSM3431036 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431036 GSM3431036 GEO Accession GSM3431036 SRX4891705 GSM3431037 GSM3431037: XX_IVD_ag7-1_S40_L004; Mus musculus; RNA-Seq SRP165841 SRS3938800 GSM3431037 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431037 GSM3431037 GEO Accession GSM3431037 SRX4891706 GSM3431038 GSM3431038: XX_IVD_ag7-2_S41_L004; Mus musculus; RNA-Seq SRP165841 SRS3938801 GSM3431038 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431038 GSM3431038 GEO Accession GSM3431038 SRX4891707 GSM3431039 GSM3431039: XX_IVD_ag7-3_S42_L004; Mus musculus; RNA-Seq SRP165841 SRS3938802 GSM3431039 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431039 GSM3431039 GEO Accession GSM3431039 SRX4891708 GSM3431040 GSM3431040: XX_d2EpliLC-1_S7_L001; Mus musculus; RNA-Seq SRP165841 SRS3938803 GSM3431040 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431040 GSM3431040 GEO Accession GSM3431040 SRX4891709 GSM3431041 GSM3431041: XX_d2EpliLC-2_S8_L001; Mus musculus; RNA-Seq SRP165841 SRS3938804 GSM3431041 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431041 GSM3431041 GEO Accession GSM3431041 SRX4891710 GSM3431042 GSM3431042: XX_d6PGCLC-1_S13_L002; Mus musculus; RNA-Seq SRP165841 SRS3938805 GSM3431042 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431042 GSM3431042 GEO Accession GSM3431042 SRX4891711 GSM3431043 GSM3431043: XX_d6PGCLC-2_S14_L002; Mus musculus; RNA-Seq SRP165841 SRS3938806 GSM3431043 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431043 GSM3431043 GEO Accession GSM3431043 SRX4891712 GSM3431044 GSM3431044: XX_d6PGCLC-3_S15_L002; Mus musculus; RNA-Seq SRP165841 SRS3938807 GSM3431044 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431044 GSM3431044 GEO Accession GSM3431044 SRX4891713 GSM3431045 GSM3431045: XY_ESC-1_S3_L001; Mus musculus; RNA-Seq SRP165841 SRS3938808 GSM3431045 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431045 GSM3431045 GEO Accession GSM3431045 SRX4891714 GSM3431046 GSM3431046: XY_ESC-2_S4_L001; Mus musculus; RNA-Seq SRP165841 SRS3938809 GSM3431046 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431046 GSM3431046 GEO Accession GSM3431046 SRX4891715 GSM3431047 GSM3431047: XY_IVD_ag3-1_S25_L003; Mus musculus; RNA-Seq SRP165841 SRS3938810 GSM3431047 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431047 GSM3431047 GEO Accession GSM3431047 SRX4891716 GSM3431048 GSM3431048: XY_IVD_ag3-2_S26_L003; Mus musculus; RNA-Seq SRP165841 SRS3938811 GSM3431048 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431048 GSM3431048 GEO Accession GSM3431048 SRX4891717 GSM3431049 GSM3431049: XY_IVD_ag3-3_S27_L003; Mus musculus; RNA-Seq SRP165841 SRS3938812 GSM3431049 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431049 GSM3431049 GEO Accession GSM3431049 SRX4891718 GSM3431050 GSM3431050: XY_IVD_ag5-1_S34_L003; Mus musculus; RNA-Seq SRP165841 SRS3938813 GSM3431050 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431050 GSM3431050 GEO Accession GSM3431050 SRX4891719 GSM3431051 GSM3431051: XY_IVD_ag5-2_S35_L003; Mus musculus; RNA-Seq SRP165841 SRS3938853 GSM3431051 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431051 GSM3431051 GEO Accession GSM3431051 SRX4891721 GSM3431052 GSM3431052: XY_IVD_ag5-3_S36_L003; Mus musculus; RNA-Seq SRP165841 SRS3938815 GSM3431052 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431052 GSM3431052 GEO Accession GSM3431052 SRX4891722 GSM3431053 GSM3431053: XY_IVD_ag7-1_S43_L004; Mus musculus; RNA-Seq SRP165841 SRS3938816 GSM3431053 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431053 GSM3431053 GEO Accession GSM3431053 SRX4891723 GSM3431054 GSM3431054: XY_IVD_ag7-2_S44_L004; Mus musculus; RNA-Seq SRP165841 SRS3938817 GSM3431054 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431054 GSM3431054 GEO Accession GSM3431054 SRX4891724 GSM3431055 GSM3431055: XY_IVD_ag7-3_S45_L004; Mus musculus; RNA-Seq SRP165841 SRS3938818 GSM3431055 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431055 GSM3431055 GEO Accession GSM3431055 SRX4891725 GSM3431056 GSM3431056: XY_d2EpiLC-1_S9_L001; Mus musculus; RNA-Seq SRP165841 SRS3938819 GSM3431056 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431056 GSM3431056 GEO Accession GSM3431056 SRX4891726 GSM3431057 GSM3431057: XY_d2EpiLC-2_S10_L001; Mus musculus; RNA-Seq SRP165841 SRS3938821 GSM3431057 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431057 GSM3431057 GEO Accession GSM3431057 SRX4891727 GSM3431058 GSM3431058: XY_d6PGCLC-1_S16_L002; Mus musculus; RNA-Seq SRP165841 SRS3938820 GSM3431058 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431058 GSM3431058 GEO Accession GSM3431058 SRX4891728 GSM3431059 GSM3431059: XY_d6PGCLC-2_S17_L002; Mus musculus; RNA-Seq SRP165841 SRS3938822 GSM3431059 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431059 GSM3431059 GEO Accession GSM3431059 SRX4891729 GSM3431060 GSM3431060: XY_d6PGCLC-3_S18_L002; Mus musculus; RNA-Seq SRP165841 SRS3938823 GSM3431060 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431060 GSM3431060 GEO Accession GSM3431060 SRX4891730 GSM3431061 GSM3431061: XO_IVD_ag11-1_S9_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938824 GSM3431061 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431061 GSM3431061 GEO Accession GSM3431061 SRX4891731 GSM3431062 GSM3431062: XO_IVD_ag11-2_S10_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938825 GSM3431062 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431062 GSM3431062 GEO Accession GSM3431062 SRX4891732 GSM3431063 GSM3431063: XO_IVD_ag13-1_S15_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938826 GSM3431063 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431063 GSM3431063 GEO Accession GSM3431063 SRX4891733 GSM3431064 GSM3431064: XO_IVD_ag13-2_S16_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938827 GSM3431064 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431064 GSM3431064 GEO Accession GSM3431064 SRX4891734 GSM3431065 GSM3431065: XO_IVD_ag9-1_S3_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938828 GSM3431065 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431065 GSM3431065 GEO Accession GSM3431065 SRX4891735 GSM3431066 GSM3431066: XO_IVD_ag9-2_S4_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938829 GSM3431066 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431066 GSM3431066 GEO Accession GSM3431066 SRX4891736 GSM3431067 GSM3431067: XX_IVD_ag11-1_S7_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938830 GSM3431067 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431067 GSM3431067 GEO Accession GSM3431067 SRX4891737 GSM3431068 GSM3431068: XX_IVD_ag11-2_S8_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938831 GSM3431068 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431068 GSM3431068 GEO Accession GSM3431068 SRX4891738 GSM3431069 GSM3431069: XX_IVD_ag13-1_S13_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938832 GSM3431069 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431069 GSM3431069 GEO Accession GSM3431069 SRX4891739 GSM3431070 GSM3431070: XX_IVD_ag13-2_S14_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938833 GSM3431070 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431070 GSM3431070 GEO Accession GSM3431070 SRX4891740 GSM3431071 GSM3431071: XX_IVD_ag9-1_S1_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938834 GSM3431071 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431071 GSM3431071 GEO Accession GSM3431071 SRX4891741 GSM3431072 GSM3431072: XX_IVD_ag9-2_S26_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938835 GSM3431072 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431072 GSM3431072 GEO Accession GSM3431072 SRX4891742 GSM3431073 GSM3431073: XY_IVD_ag11-1_S11_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938836 GSM3431073 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431073 GSM3431073 GEO Accession GSM3431073 SRX4891743 GSM3431074 GSM3431074: XY_IVD_ag11-2_S12_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938838 GSM3431074 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431074 GSM3431074 GEO Accession GSM3431074 SRX4891744 GSM3431075 GSM3431075: XY_IVD_ag13-1_S17_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938837 GSM3431075 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431075 GSM3431075 GEO Accession GSM3431075 SRX4891745 GSM3431076 GSM3431076: XY_IVD_ag13-2_S18_L002; Mus musculus; ssRNA-seq SRP165841 SRS3938839 GSM3431076 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431076 GSM3431076 GEO Accession GSM3431076 SRX4891746 GSM3431077 GSM3431077: XY_IVD_ag9-1_S5_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938840 GSM3431077 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431077 GSM3431077 GEO Accession GSM3431077 SRX4891747 GSM3431078 GSM3431078: XY_IVD_ag9-2_S6_L001; Mus musculus; ssRNA-seq SRP165841 SRS3938841 GSM3431078 RNA-Seq TRANSCRIPTOMIC cDNA Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared. Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina). Illumina HiSeq 2500 gds 303431078 GSM3431078 GEO Accession GSM3431078