SRS4072094 SAMN10458100 1969832 ciliate metagenome cultivation medium for ciliate bioproject 505416 PRJNA505416 host Paramecium caudatum isolation_source freshwater pond collection_date 2015-11 geo_loc_name Russia: Primorsky Krai,Vladivostok, freshwater pond lat_lon 43.1162 N 131.9380 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process The cultivation medium used for maintenance of the ciliate culture was taken as a control; 1 ml of every medium was concentrated by centrifugation at 10,000 rpm for 10 minutes, the pellets with 10 μl of liquid were transferred into PCR tubes, 50 μl of 96% ethanol were added, and the samples were stored at −20° C till DNA extraction. samp_size 1 ml source_material_id ME4c primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072095 SAMN10458105 1969832 ciliate metagenome single ciliate cell bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 1 cell source_material_id BGpl2014_1_86 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072096 SAMN10458104 1969832 ciliate metagenome single ciliate cell bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 1 cell source_material_id BGpl2014_1_83 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072097 SAMN10458101 1969832 ciliate metagenome planktonic sample bioproject 505416 PRJNA505416 host not applicable isolation_source brackish lake collection_date 2015-10-07 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile flasks samp_mat_process Each sample was filtered through cellulose nitrate membrane with diameter of pores 0,22 µm immediately after sampling. Total DNA was extracted by mechanic homogenization with Lysing matrix E added by chemical extraction. samp_size 100 ml source_material_id BGpl2015_2_62 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072098 SAMN10458099 1969832 ciliate metagenome single ciliate cells bioproject 505416 PRJNA505416 host Paramecium caudatum isolation_source freshwater pond collection_date 2015-11 geo_loc_name Russia: Primorsky Krai,Vladivostok, freshwater pond lat_lon 43.1162 N 131.9380 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 100 cells source_material_id 100 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072099 SAMN10458098 1969832 ciliate metagenome cultivation medium for ciliate bioproject 505416 PRJNA505416 host Paramecium caudatum isolation_source freshwater creek collection_date 2015-10 geo_loc_name Russia: Saint-Petersburg, Peterhof, freshwater creek lat_lon 59.8796 N 29.8641 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process The cultivation medium used for maintenance of the ciliate culture was taken as a control; 1 ml of every medium was concentrated by centrifugation at 10,000 rpm for 10 minutes, the pellets with 10 μl of liquid were transferred into PCR tubes, 50 μl of 96% ethanol were added, and the samples were stored at −20° C till DNA extraction. samp_size 1 ml source_material_id ME3c primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072100 SAMN10458097 1969832 ciliate metagenome single ciliate cells bioproject 505416 PRJNA505416 host Paramecium caudatum isolation_source freshwater creek collection_date 2015-10 geo_loc_name Russia: Saint-Petersburg, Peterhof, freshwater creek lat_lon 59.8796 N 29.8641 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 5 cells source_material_id 3c primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072101 SAMN10458096 1969832 ciliate metagenome planktonic sample bioproject 505416 PRJNA505416 host not applicable isolation_source freshwater creek collection_date 2015-10 geo_loc_name Russia: Saint-Petersburg, Peterhof, freshwater creek lat_lon 59.8796 N 29.8641 E rel_to_oxygen aerobe samp_collect_device sterile flasks samp_mat_process Each sample was filtered through cellulose nitrate membrane with diameter of pores 0,22 µm immediately after sampling. Total DNA was extracted by mechanic homogenization with Lysing matrix E added by chemical extraction. samp_size 100 ml source_material_id PW5 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072102 SAMN10458103 1969832 ciliate metagenome cultivation medium for ciliate bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process The cultivation medium used for maintenance of the ciliate culture was taken as a control; 1 ml of every medium was concentrated by centrifugation at 10,000 rpm for 10 minutes, the pellets with 10 μl of liquid were transferred into PCR tubes, 50 μl of 96% ethanol were added, and the samples were stored at −20° C till DNA extraction. samp_size 1 ml source_material_id BGpl2014_1_80 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072103 SAMN10458102 1969832 ciliate metagenome single ciliate cells bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 10 cells source_material_id BGpl2014_1_78 primers F-S-D-Bact-0341-b-S-17 and R-S-D-Bact-0785-a-A-21 (Klindworth et al., 2013) BioSampleModel Metagenome or environmental SRS4072104 SAMN10458107 1969832 ciliate metagenome cultivation medium for ciliate bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process The cultivation medium used for maintenance of the ciliate culture was taken as a control; 1 ml of every medium was concentrated by centrifugation at 10,000 rpm for 10 minutes, the pellets with 10 μl of liquid were transferred into PCR tubes, 50 μl of 96% ethanol were added, and the samples were stored at −20° C till DNA extraction. samp_size 1 ml source_material_id BGpl2014_1_89 primers F515 and R907 (Zhou et al., 2011) BioSampleModel Metagenome or environmental SRS4072105 SAMN10458106 1969832 ciliate metagenome single ciliate cells bioproject 505416 PRJNA505416 host Paramecium aurelia isolation_source brackish lake collection_date 2014-08-10 geo_loc_name Russia: Orenburg Region, Sol-Iletsk, Bolshoe Gorodskoe Lake lat_lon 51.1531 N 54.9988 E rel_to_oxygen aerobe samp_collect_device sterile tube samp_mat_process Single cells of ciliates were isolated from the samples, passed three to five times through aliquots of sterile water and then kept there for 6 hours in order to digest and expulse all contents of their food vacuoles. Thereafter, ciliates were passed through sterile distilled water, transferred into PCR tubes with 10 μl of this water, 50 μl of 96% ethanol were added, and samples were stored at −20° C till DNA extraction. samp_size 10 cells source_material_id BGpl2014_1_88 primers F515 and R907 (Zhou et al., 2011) BioSampleModel Metagenome or environmental