SRX5097743 Sen16 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108496 Sen16 Sen16 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097744 Sen15 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108497 Sen15 Sen15 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097745 Sen19 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108498 Sen19 Sen19 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097746 Sen18 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108499 Sen18 Sen18 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097747 Sen12 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108500 Sen12 Sen12 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097748 Sen11 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108501 Sen11 Sen11 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097749 Sen14 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108502 Sen14 Sen14 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097750 Sen13 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108503 Sen13 Sen13 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097751 Sen21 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108504 Sen21 Sen21 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097752 Sen20 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108505 Sen20 Sen20 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097753 Sen06 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108506 Sen06 Sen06 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097754 Sen05 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108507 Sen05 Sen05 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097755 Sen08 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108508 Sen08 Sen08 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097756 Sen07 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108509 Sen07 Sen07 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097757 Sen02 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108510 Sen02 Sen02 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097758 Sen01 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108511 Sen01 Sen01 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097759 Sen04 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108512 Sen04 Sen04 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097760 Sen03 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108513 Sen03 Sen03 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097761 Sen10 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108514 Sen10 Sen10 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097762 Sen09 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108515 Sen09 Sen09 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097763 Sen26 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108516 Sen26 Sen26 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097764 Sen22 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108517 Sen22 Sen22 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097765 Sen23 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108518 Sen23 Sen23 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097766 Sen24 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108519 Sen24 Sen24 AMPLICON METAGENOMIC PCR Ion Torrent PGM SRX5097767 Sen25 SRP172551 bp0 The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines. SRS4108520 Sen25 Sen25 AMPLICON METAGENOMIC PCR Ion Torrent PGM