SRX5100892 GSM3503226 SRP172745 SRS4111191 GSM3503226 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503226 GSM3503226 GEO Accession GSM3503226 SRX5100893 GSM3503227 SRP172745 SRS4111192 GSM3503227 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503227 GSM3503227 GEO Accession GSM3503227 SRX5100894 GSM3503228 SRP172745 SRS4111193 GSM3503228 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503228 GSM3503228 GEO Accession GSM3503228 SRX5100895 GSM3503229 SRP172745 SRS4111194 GSM3503229 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503229 GSM3503229 GEO Accession GSM3503229 SRX5100896 GSM3503230 SRP172745 SRS4111195 GSM3503230 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503230 GSM3503230 GEO Accession GSM3503230 SRX5100897 GSM3503231 SRP172745 SRS4111196 GSM3503231 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503231 GSM3503231 GEO Accession GSM3503231 SRX5100898 GSM3503232 SRP172745 SRS4111197 GSM3503232 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503232 GSM3503232 GEO Accession GSM3503232 SRX5100899 GSM3503233 SRP172745 SRS4111198 GSM3503233 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503233 GSM3503233 GEO Accession GSM3503233 SRX5100900 GSM3503234 SRP172745 SRS4111199 GSM3503234 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503234 GSM3503234 GEO Accession GSM3503234 SRX5100901 GSM3503235 SRP172745 SRS4111200 GSM3503235 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503235 GSM3503235 GEO Accession GSM3503235 SRX5100902 GSM3503236 SRP172745 SRS4111201 GSM3503236 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503236 GSM3503236 GEO Accession GSM3503236 SRX5100903 GSM3503237 SRP172745 SRS4111202 GSM3503237 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503237 GSM3503237 GEO Accession GSM3503237 SRX5100904 GSM3503238 SRP172745 SRS4111203 GSM3503238 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503238 GSM3503238 GEO Accession GSM3503238 SRX5100905 GSM3503239 SRP172745 SRS4111204 GSM3503239 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503239 GSM3503239 GEO Accession GSM3503239 SRX5100906 GSM3503240 SRP172745 SRS4111205 GSM3503240 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503240 GSM3503240 GEO Accession GSM3503240 SRX5100907 GSM3503241 SRP172745 SRS4111206 GSM3503241 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503241 GSM3503241 GEO Accession GSM3503241 SRX5100908 GSM3503242 SRP172745 SRS4111207 GSM3503242 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503242 GSM3503242 GEO Accession GSM3503242 SRX5100909 GSM3503243 SRP172745 SRS4111208 GSM3503243 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503243 GSM3503243 GEO Accession GSM3503243 SRX5100910 GSM3503244 SRP172745 SRS4111209 GSM3503244 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503244 GSM3503244 GEO Accession GSM3503244 SRX5100911 GSM3503245 SRP172745 SRS4111210 GSM3503245 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503245 GSM3503245 GEO Accession GSM3503245 SRX5100912 GSM3503246 SRP172745 SRS4111211 GSM3503246 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503246 GSM3503246 GEO Accession GSM3503246 SRX5100913 GSM3503247 SRP172745 SRS4111212 GSM3503247 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503247 GSM3503247 GEO Accession GSM3503247 SRX5100914 GSM3503248 SRP172745 SRS4111213 GSM3503248 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503248 GSM3503248 GEO Accession GSM3503248 SRX5100915 GSM3503249 SRP172745 SRS4111214 GSM3503249 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503249 GSM3503249 GEO Accession GSM3503249 SRX5100916 GSM3503250 SRP172745 SRS4111215 GSM3503250 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503250 GSM3503250 GEO Accession GSM3503250 SRX5100917 GSM3503251 SRP172745 SRS4111216 GSM3503251 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503251 GSM3503251 GEO Accession GSM3503251 SRX5100918 GSM3503252 SRP172745 SRS4111217 GSM3503252 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503252 GSM3503252 GEO Accession GSM3503252 SRX5100919 GSM3503253 SRP172745 SRS4111218 GSM3503253 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503253 GSM3503253 GEO Accession GSM3503253 SRX5100920 GSM3503254 SRP172745 SRS4111219 GSM3503254 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503254 GSM3503254 GEO Accession GSM3503254 SRX5100921 GSM3503255 SRP172745 SRS4111220 GSM3503255 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted from peripheral blood mononuclear cells of CLL patients and 19+ B cells of healthy controls using the mirVana miRNA isolation kit (Thermofisher Scientific, MA, USA) . RNA quality/quantity and miRNA quality/quantity was assessed using RNA 6000 Nano kit and small RNA kit respectively (Agilent technologogies, Santa Clara, USA) on Agilent 2100 bioanalyzer. For sequencing, libraries were generated using the IlluminaâTruSeqÔsmall RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, RNA adapters were ligated to 1 mg of total RNA. The ligated RNA was reverse transcribed to generate cDNA using RT primer, dNTP Mix, DTT, RNase Inhibitor and SuperScript II Reverse Transcriptase. The cDNA libraries were amplified using PCR to selectively enrich RNA fragments with adapter molecules. The resulting PCR products were purified using Ampure XP magnetic beads (Beckman Coulter) and run on a pre-cast agarose gel (Thermofisher Scientific, MA, USA). The gels were then extracted along the 160 bp RNA ladder band and the bottom of the 145 bp from the gel using the Mini Elute Qiagen Gel-Extraction Kit following the manufacturer's protocol (Qiagen, CA, USA). The quality and concentration of the libraries was assessed using the Qubit (Thermofisher Scientific, MA, USA) and DNA high sensitivity chip (Agilent Technologies, Santa Clara, USA). The libraries were then mixed at equimolar concentration. NextSeq 500 gds 303503255 GSM3503255 GEO Accession GSM3503255