SRP173938 PRJNA510811 High Density Transposon Mutagenesis in conjugative plasmid TP114 High Density Transposon Mutagenesis was performed on Escherichia coli Nissle1917 bearing TP114 to generate a mutants library. Gene essentiality for plasmid maintenance, conjugative transfer on agar plate, and conjugative transfer in the mouse gut was next investigated using the mutant library.