SRX5171463 GSM3525823 SRP174097 SRS4179086 GSM3525823 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525823 GSM3525823 GEO Accession GSM3525823 SRX5171464 GSM3525825 SRP174097 SRS4179085 GSM3525825 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525825 GSM3525825 GEO Accession GSM3525825 SRX5171465 GSM3525826 SRP174097 SRS4179087 GSM3525826 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525826 GSM3525826 GEO Accession GSM3525826 SRX5171466 GSM3525828 SRP174097 SRS4179088 GSM3525828 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525828 GSM3525828 GEO Accession GSM3525828 SRX5171467 GSM3525830 SRP174097 SRS4179089 GSM3525830 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525830 GSM3525830 GEO Accession GSM3525830 SRX5171468 GSM3525831 SRP174097 SRS4179090 GSM3525831 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525831 GSM3525831 GEO Accession GSM3525831 SRX5171469 GSM3525833 SRP174097 SRS4179091 GSM3525833 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525833 GSM3525833 GEO Accession GSM3525833 SRX5171470 GSM3525835 SRP174097 SRS4179092 GSM3525835 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525835 GSM3525835 GEO Accession GSM3525835 SRX5171471 GSM3525837 SRP174097 SRS4179093 GSM3525837 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525837 GSM3525837 GEO Accession GSM3525837 SRX5171472 GSM3525838 SRP174097 SRS4179094 GSM3525838 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525838 GSM3525838 GEO Accession GSM3525838 SRX5171473 GSM3525840 SRP174097 SRS4179095 GSM3525840 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525840 GSM3525840 GEO Accession GSM3525840 SRX5171474 GSM3525842 SRP174097 SRS4179096 GSM3525842 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525842 GSM3525842 GEO Accession GSM3525842 SRX5171475 GSM3525844 SRP174097 SRS4179097 GSM3525844 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525844 GSM3525844 GEO Accession GSM3525844 SRX5171476 GSM3525845 SRP174097 SRS4179098 GSM3525845 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525845 GSM3525845 GEO Accession GSM3525845 SRX5171477 GSM3525847 SRP174097 SRS4179099 GSM3525847 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525847 GSM3525847 GEO Accession GSM3525847 SRX5171478 GSM3525849 SRP174097 SRS4179100 GSM3525849 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525849 GSM3525849 GEO Accession GSM3525849 SRX5171479 GSM3525851 SRP174097 SRS4179101 GSM3525851 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525851 GSM3525851 GEO Accession GSM3525851 SRX5171480 GSM3525853 SRP174097 SRS4179102 GSM3525853 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525853 GSM3525853 GEO Accession GSM3525853 SRX5171481 GSM3525854 SRP174097 SRS4179103 GSM3525854 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525854 GSM3525854 GEO Accession GSM3525854 SRX5171482 GSM3525856 SRP174097 SRS4179104 GSM3525856 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525856 GSM3525856 GEO Accession GSM3525856 SRX5171483 GSM3525858 SRP174097 SRS4179105 GSM3525858 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525858 GSM3525858 GEO Accession GSM3525858 SRX5171484 GSM3525859 SRP174097 SRS4179106 GSM3525859 RNA-Seq TRANSCRIPTOMIC cDNA Freshly isolated livers were immediately frozen and stored in liquid nitrogen until use. mRNA was extracted using Trizol regent (Invitrogen) following the manufacturer's instructions. For mRNA-seq analysis, mRNA was isolated with Dynabeads® mRNA Purification Kits (Invitrogen), fragmented in 5x first strand buffer(Invitrogen) at 95°C for 5 min, and reverse-transcribed using SuperScript® II Reverse Transcriptase (Invitrogen). After the first strand was synthesized, Second Strand Buffer (Invitrogen), RNase H (New England Biolabs), dNTPs, and DNA polymerase I (TaKaRa) were added to the second-strand synthesis reaction for 2.5 h at 16°C, and cDNA was purified with Agencourt AMPure XP beads (Beckman). Libraries were constructed with a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), PCR products were purified using AMPure XP beads. RNA libraries were quantified by Qubit 1.0 (Invitrogen) and analyzed with an Agilent 2100 bioanalyzer(Agilent) for size distribution, and were sequenced with the Illumina Hiseq-2500 platform. Illumina HiSeq 2500 gds 303525859 GSM3525859 GEO Accession GSM3525859